Structural Changes of the Paraflagellar Rod during Flagellar Beating in Trypanosoma cruzi

, the agent of Chagas disease, is a protozoan member of the Kinetoplastidae family characterized for the presence of specific and unique structures that are involved in different cell activities. One of them is the paraflagellar rod (PFR), a complex array of filaments connected to the flagellar axoneme. Although the function played by the PFR is not well established, it has been shown that silencing of the synthesis of its major proteins by either knockout of RNAi impairs and/or modifies the flagellar motility.Here, we present results obtained by atomic force microscopy (AFM) and transmission electron microscopy (TEM) of replicas of quick-frozen, freeze-fractured, deep-etched and rotary-replicated cells to obtain detailed information of the PFR structures in regions of the flagellum in straight and in bent state. The images obtained show that the PFR is not a fixed and static structure. The pattern of organization of the PFR filament network differs between regions of the flagellum in a straight state and those in a bent state. Measurements of the distances between the PFR filaments and the filaments that connect the PFR to the axoneme as well as of the angles between the intercrossed filaments supported this idea.Graphic computation based on the information obtained allowed the proposal of an animated model for the PFR structure during flagellar beating and provided a new way of observing PFR filaments during flagellar beating

Description of the oocysts of three new species of 'Eimeria' (Apicomplexa: Eimeriidae) from Iguanid lizards (Sauria: Iguanidae) of Central and South America

Three new species of Eimeria are described from iguanid lizards of Central and South America. The oocysts of each species have no micropyles or residua and the sporocysts lack Stieda bodies, but all have a sporocyst residuum. Eimeria sanctaluciae n.sp. was found in the St. Lucia tree lizard, Anolis luciae, collected from the Maria Islands, Lesser Antilles. The oocysts are spherical to subspherical, averaging 17.3 x 16.5 microns, with a single layered colourless wall; about 60% contain polar granules. The sporocysts are ellipsoidal and average 7.7 x 5.5 microns. Eimeria liolaemi n.sp. was recovered from the blue-gold swift, Liolaemus taenius, from Chile. The oocysts are spherical to subspherical, measuring 21 x 20.1 microns with a single-layered colourless wall. The sporocysts are subspherical and average 7.4 x 6.8 microns. Eimeria caesicia n.sp. is described from the Brazilian collared iguanid, Tropidurus torquatus. The oocysts measure 27.4 x 23.7 microns, are spherical to subspherical, with a bilayered wall, the outer surface of which appears pale blue in colour, the thin, inner wall appearing brown, when viewed by direct light under the optical microscope. The sporocysts are subspherical and average 9.4 x 7.2 microns. Unnamed polysporocystid oocysts with dizoic sporocysts are reported from the faeces of the lesser St. Vincent tree lizard, Anolis trinitatis and the possibility of spurious parasitism briefly discussed. In addition, oocysts of an unnamed Isospora sp. with a smooth oocyst wall which closely resembles I. reui were recovered from A. trinitatis

Implementing Environmental Flows in Complex Water Resources Systems Case Study: The Duero River Basin, Spain

European river basin authorities are responsible for the implementation of the new river basin management plans in accordance with the European Water Framework Directive. This paper presents a new methodology framework and approach to define and evaluate environmental flow regimes in the realistic complexities that exist with multiple water resource needs at a basin scale. This approach links river basin simulation models and habitat time series analysis to generate ranges of environmental flows (e-flows), which are evaluated by using habitat, hydropower production and reliability of water supply criteria to produce best possible alternatives. With the use of these tools, the effects of the proposed e-flows have been assessed to help in the consultation process. The possible effects analysed are impacts on water supply reliability, hydropower production and aquatic habitat. After public agreements, a heuristic optimization process was applied to maximize e-flows and habitat indicators, while maintaining a legal level of reliability for water resource demands. The final optimal e-flows were considered for the river basin management plans of the Duero river basin. This paper demonstrates the importance of considering quantitative hydrologic and ecological aspects of e-flows at the basin scale in addressing complex water resource systems. This approach merges standard methods such as physical habitat simulations and time series analyses for evaluating alternatives, with recent methods to simulate and optimize water management alternatives in river networks. It can be integrated with or used to complement other frameworks for e-flow assessments such as the In-stream Flow Incremental Methodology and Ecological Limits of Hydrologic Alteration.We are grateful to Professor Jay Lund and the reviewers for their very helpful comments and suggestions. We thank the Confederacion Hidrografica del Duero (Spanish Ministry of Environment) for the data provided to develop this study and the Spanish Ministry of Science and Innovation (Comision Interministerial de Ciencia y Tecnologia, CICYT) for the funding projects, INTEGRAME (contract CGL2009-11798) and SCARCE (programme Consolider-Ingenio 2010, project CSD2009-00065).Paredes Arquiola, J.; Martinez-Capel, F.; Solera Solera, A.; Aguilella Vidal, V. (2011). Implementing Environmental Flows in Complex Water Resources Systems Case Study: The Duero River Basin, Spain. River Research and Applications. 29(4):451-468. doi:10.1002/rra.1617S45146829