Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4+CD25+FoxP3+ regulatory T cells activation

Rationale: Loss of histone macroH2A1 induces appearance of cancer stem cells (CSCs)-like cells in hepatocellular carcinoma (HCC). How CSCs interact with the tumor microenvironment and the adaptive immune system is unclear. Methods: We screened aggressive human HCC for macroH2A1 and CD44 CSC marker expression. We also knocked down (KD) macroH2A1 in HCC cells, and performed integrated transcriptomic and secretomic analyses. Results: Human HCC showed low macroH2A1 and high CD44 expression compared to control tissues. MacroH2A1 KD CSC-like cells transferred paracrinally their chemoresistant properties to parental HCC cells. MacroH2A1 KD conditioned media transcriptionally reprogrammed parental HCC cells activated regulatory CD4+/CD25+/FoxP3+ T cells (Tregs). Conclusions: Loss of macroH2A1 in HCC cells drives cancer stem-cell propagation and evasion from immune surveillance

The Gattini cameras for optical sky brightness measurements at Dome C, Antarctica

The Gattini cameras are two site testing instruments for the measurement of optical sky brightness, large area cloud cover and auroral detection of the night sky above the high altitude Dome C site in Antarctica. The cameras have been operating since installation in January 2006 and are currently at the end of the first Antarctic winter season. The cameras are transit in nature and are virtually identical both adopting Apogee Alta CCD detectors. By taking frequent images of the night sky we obtain long term cloud cover statistics, measure the sky background intensity as a function of solar and lunar altitude and phase and directly measure the spatial extent of bright aurora if present and when they occur. The full data set will return in December 2006 however a limited amount of data has been transferred via the Iridium network enabling preliminary data reduction and system evaluation. An update of the project is presented together with preliminary results from data taken since commencement of the winter season

The massive protostar W43-MM1 as seen by Herschel-HIFI water spectra: high turbulence and accretion luminosity

We present Herschel/HIFI observations of fourteen water lines in W43-MM1, a massive protostellar object in the luminous star cluster-forming region W43. We analyze the gas dynamics from the line profiles using Herschel-HIFI observations (WISH-KP) of fourteen far-IR water lines (H2O, H217O, H218O), CS(11-10), and C18O(9-8) lines, and using our modeling of the continuum spectral energy distribution. As for lower mass protostellar objects, the molecular line profiles are a mix of emission and absorption, and can be decomposed into 'medium', and 'broad' velocity components. The broad component is the outflow associated with protostars of all masses. Our modeling shows that the remainder of the water profiles can be well fitted by an infalling and passively heated envelope, with highly supersonic turbulence varying from 2.2 km/s in the inner region to 3.5 km/s in the outer envelope. Also, W43-MM1 has a high accretion rate, between 4.0 x 10^{-4} and 4.0 x 10^{-2} \msun /yr, derived from the fast (0.4-2.9 km/s) infall observed. We estimate a lower mass limit of gaseous water of 0.11 \msun and total water luminosity of 1.5 \lsun (in the 14 lines presented here). The central hot core is detected with a water abundance of 1.4 x 10^{-4} while the water abundance for the outer envelope is 8 x10^{-8}. The latter value is higher than in other sources, most likely related to the high turbulence and the micro-shocks created by its dissipation. Examining water lines of various energies, we find that the turbulent velocity increases with the distance to the center. While not in clear disagreement with the competitive accretion scenario, this behavior is predicted by the turbulent core model. Moreover, the estimated accretion rate is high enough to overcome the expected radiation pressure.Comment: Accepted in A&A on April 2, 2012. 12 pages 7 figure

A Measurement of the Cosmic Microwave Background Damping Tail from the 2500-square-degree SPT-SZ survey

We present a measurement of the cosmic microwave background (CMB) temperature power spectrum using data from the recently completed South Pole Telescope Sunyaev-Zel'dovich (SPT-SZ) survey. This measurement is made from observations of 2540 deg$^2$ of sky with arcminute resolution at $150\,$GHz, and improves upon previous measurements using the SPT by tripling the sky area. We report CMB temperature anisotropy power over the multipole range $650<\ell<3000$. We fit the SPT bandpowers, combined with the seven-year Wilkinson Microwave Anisotropy Probe (WMAP7) data, with a six-parameter LCDM cosmological model and find that the two datasets are consistent and well fit by the model. Adding SPT measurements significantly improves LCDM parameter constraints; in particular, the constraint on $\theta_s$ tightens by a factor of 2.7. The impact of gravitational lensing is detected at $8.1\, \sigma$, the most significant detection to date. This sensitivity of the SPT+WMAP7 data to lensing by large-scale structure at low redshifts allows us to constrain the mean curvature of the observable universe with CMB data alone to be $\Omega_k=-0.003^{+0.014}_{-0.018}$. Using the SPT+WMAP7 data, we measure the spectral index of scalar fluctuations to be $n_s=0.9623 \pm 0.0097$ in the LCDM model, a $3.9\,\sigma$ preference for a scale-dependent spectrum with $n_s<1$. The SPT measurement of the CMB damping tail helps break the degeneracy that exists between the tensor-to-scalar ratio $r$ and $n_s$ in large-scale CMB measurements, leading to an upper limit of $r<0.18$ (95%,C.L.) in the LCDM+$r$ model. Adding low-redshift measurements of the Hubble constant ($H_0$) and the baryon acoustic oscillation (BAO) feature to the SPT+WMAP7 data leads to further improvements. The combination of SPT+WMAP7+$H_0$+BAO constrains $n_s=0.9538 \pm 0.0081$ in the LCDM model, a $5.7\,\sigma$ detection of $n_s < 1$, ... [abridged]Comment: 21 pages, 10 figures. Replaced with version accepted by ApJ. Data products are available at http://pole.uchicago.edu/public/data/story12

A Direct Measurement of the Linear Bias of Mid-infrared-selected Quasars at z ap 1 Using Cosmic Microwave Background Lensing

We measure the cross-power spectrum of the projected mass density as traced by the convergence of the cosmic microwave background lensing field from the South Pole Telescope (SPT) and a sample of Type 1 and 2 (unobscured and obscured) quasars at langzrang ~ 1 selected with the Wide-field Infrared Survey Explorer, over 2500 deg2. The cross-power spectrum is detected at ≈7σ, and we measure a linear bias b = 1.61 ± 0.22, consistent with clustering analyses. Using an independent lensing map, derived from Planck observations, to measure the cross-spectrum, we find excellent agreement with the SPT analysis. The bias of the combined sample of Type 1 and 2 quasars determined in this work is similar to that previously determined for Type 1 quasars alone; we conclude that obscured and unobscured quasars trace the matter field in a similar way. This result has implications for our understanding of quasar unification and evolution schemes.Peer reviewe

The 10 Meter South Pole Telescope

The South Pole Telescope (SPT) is a 10 m diameter, wide-field, offset Gregorian telescope with a 966-pixel, multi-color, millimeter-wave, bolometer camera. It is located at the Amundsen-Scott South Pole station in Antarctica. The design of the SPT emphasizes careful control of spillover and scattering, to minimize noise and false signals due to ground pickup. The key initial project is a large-area survey at wavelengths of 3, 2 and 1.3 mm, to detect clusters of galaxies via the Sunyaev-Zeldovich effect and to measure the small-scale angular power spectrum of the cosmic microwave background (CMB). The data will be used to characterize the primordial matter power spectrum and to place constraints on the equation of state of dark energy. A second-generation camera will measure the polarization of the CMB, potentially leading to constraints on the neutrino mass and the energy scale of inflation.Comment: 47 pages, 14 figures, updated to match version to be published in PASP 123 903 (May, 2011

A CMB lensing mass map and its correlation with the cosmic infrared background

We use a temperature map of the cosmic microwave background (CMB) obtained using the South Pole Telescope at 150 GHz to construct a map of the gravitational convergence to z ~ 1100, revealing the fluctuations in the projected mass density. This map shows individual features that are significant at the ~ 4 sigma level, providing the first image of CMB lensing convergence. We cross-correlate this map with Herschel/SPIRE maps covering 90 square degrees at wavelengths of 500, 350, and 250 microns. We show that these submillimeter-wavelength (submm) maps are strongly correlated with the lensing convergence map, with detection significances in each of the three submm bands ranging from 6.7 to 8.8 sigma. We fit the measurement of the cross power spectrum assuming a simple constant bias model and infer bias factors of b=1.3-1.8, with a statistical uncertainty of 15%, depending on the assumed model for the redshift distribution of the dusty galaxies that are contributing to the Herschel/SPIRE maps.Comment: 5 pages, 3 figures, to be submitted to ApJ

Extragalactic millimeter-wave point source catalog, number counts and statistics from 771 square degrees of the SPT-SZ Survey

We present a point source catalog from 771 square degrees of the South Pole Telescope Sunyaev Zel'dovich (SPT-SZ) survey at 95, 150, and 220 GHz. We detect 1545 sources above 4.5 sigma significance in at least one band. Based on their relative brightness between survey bands, we classify the sources into two populations, one dominated by synchrotron emission from active galactic nuclei, and one dominated by thermal emission from dust-enshrouded star-forming galaxies. We find 1238 synchrotron and 307 dusty sources. We cross-match all sources against external catalogs and find 189 unidentified synchrotron sources and 189 unidentified dusty sources. The dusty sources without counterparts are good candidates for high-redshift, strongly lensed submillimeter galaxies. We derive number counts for each population from 1 Jy down to roughly 9, 5, and 11 mJy at 95, 150, and 220 GHz. We compare these counts with galaxy population models and find that none of the models we consider for either population provide a good fit to the measured counts in all three bands. The disparities imply that these measurements will be an important input to the next generation of millimeter-wave extragalactic source population models.Comment: 23 pages, 8 figures, submitted to Ap

A Comparison of Maps and Power Spectra Determined from South Pole Telescope and Planck Data

We study the consistency of 150 GHz data from the South Pole Telescope (SPT) and 143 GHz data from the Planck satellite over the patch of sky covered by the SPT-SZ survey. We first visually compare the maps and find that the residuals appear consistent with noise after accounting for differences in angular resolution and filtering. We then calculate (1) the cross-spectrum between two independent halves of SPT data, (2) the cross-spectrum between two independent halves of Planck data, and (3) the cross-spectrum between SPT and Planck data. We find the three cross-spectra are well-fit (PTE = 0.30) by the null hypothesis in which both experiments have measured the same sky map up to a single free calibration parameter---i.e., we find no evidence for systematic errors in either data set. As a by-product, we improve the precision of the SPT calibration by nearly an order of magnitude, from 2.6% to 0.3% in power. Finally, we compare all three cross-spectra to the full-sky Planck power spectrum and find marginal evidence for differences between the power spectra from the SPT-SZ footprint and the full sky. We model these differences as a power law in spherical harmonic multipole number. The best-fit value of this tilt is consistent among the three cross-spectra in the SPT-SZ footprint, implying that the source of this tilt is a sample variance fluctuation in the SPT-SZ region relative to the full sky. The consistency of cosmological parameters derived from these datasets is discussed in a companion paper.Comment: 15 pages, 9 figures. Published in The Astrophysical Journal. Current arxiv version matches published versio