Cartografía temática para el ordenamiento territorial : partido de Marcos Paz

Fil: Cabral, Mirta Graciela. Cátedra de Estadística. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Marlats, Ana Laura. CICBAFil: Andrade, Gisel. CICBAFil: Muntz, Daniel E.. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Sierra, Leonardo. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Martínez, Nicolás. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Gado, Patrick. Ministerio de Asuntos Agrarios de la Provincia de Buenos Aires; Argentin

Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces

Open Access ArticleCassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich in carbohydrate, are deficient in essential micronutrients, including provitamin A carotenoids. Increasing carotenoid content in cassava storage roots is important to reduce the incidence of vitamin A deficiency, a public health problem in sub-Saharan Africa. However, cassava improvement advances slowly, mainly due to limited information on the molecular factors influencing β-carotene accumulation in cassava. To address this problem, we performed comparative transcriptomic and untargeted metabolic analyses of roots and leaves of eleven African cassava landraces ranging from white to deep yellow colour, to uncover regulators of carotenoid biosynthesis and accumulation with conserved function in yellow cassava roots. Sequence analysis confirmed the presence of a mutation, known to influence β-carotene content, in PSY transcripts of deep yellow but not of pale yellow genotypes. We identified genes and metabolites with expression and accumulation levels significantly associated with β-carotene content. Particularly an increased activity of the abscisic acid catabolism pathway together with a reduced amount of L-carnitine, may be related to the carotenoid pathway flux, higher in yellow than in white storage roots. In fact, NCED_3.1 was specifically expressed at a lower level in all yellow genotypes suggesting that it could be a potential target for increasing carotenoid accumulation in cassava. These results expand the knowledge on metabolite compositions and molecular mechanisms influencing carotenoid biosynthesis and accumulation in cassava and provide novel information for biotechnological applications and genetic improvement of cassava with high nutritional values

Adaptive downregulation of Cl- /HCO3 - exchange activity in rat hepatocytes under experimental obstructive cholestasis

In obstructive cholestasis, there is an integral adaptive response aimed to diminish the bile flow and minimize the injury of bile ducts caused by increased intraluminal pressure and harmful levels of bile salts and bilirrubin. Canalicular bicarbonate secretion, driven by the anion exchanger 2 (AE2), is an influential determinant of the canalicular bile salt-independent bile flow. In this work, we ascertained whether AE2 expression and/or activity is reduced in hepatocytes from rats with common bile duct ligation (BDL), as part of the adaptive response to cholestasis. After 4 days of BDL, we found that neither AE2 mRNA expression (measured by quantitative real-time PCR) nor total levels of AE2 protein (assessed by western blot) were modified in freshly isolated hepatocytes. However, BDL led to a decrease in the expression of AE2 protein in plasma membrane fraction as compared with SHAM control. Additionally, AE2 activity (J(OH)-, mmol/L/min), measured in primary cultured hepatocytes from BDL and SHAM rats, was decreased in the BDL group versus the control group (1.9 +/- 0.3 vs. 3.1 +/- 0.2, p<0.005). cAMP-stimulated AE2 activity, however, was not different between SHAM and BDL groups (3.7 +/- 0.3 vs. 3.5 +/- 0.3), suggesting that cAMP stimulated insertion into the canalicular membrane of AE2-containing intracellular vesicles, that had remained abnormally internalized after BDL. In conclusion, our results point to the existence of a novel adaptive mechanism in cholestasis aimed to reduce biliary pressure, in which AE2 internalization in hepatocytes might result in decreased canalicular HCO3- output and decreased bile flow.This work was supported by grants from Spanish Carlos III Health Institute (ISCIII) [J. M. Banales (FIS PI15/01132, PI18/01075 and Miguel Servet Program CON14/00129) cofinanced by "Fondo Europeo de Desarrollo Regional" (FEDER); "Instituto de Salud Carlos III" [CIBERehd: J. M. Banales], Spain; BIOEF (Basque Foundation for Innovation and Health Research: EiTB Maratoia BIO15/CA/016/BD to J. M. Banales), Department of Health of the Basque Country (J. M. Banales: 2017111010) and Euskadi RIS3 (J. M. Banales: 2016222001, 2017222014, 2018222029). "Fundacion Cientifica de la Asociacion Espanola Contra el Cancer" (AECC Scientific Foundation, to J. M. Banales). F. A. Crocenzi was recipient of a Young Investigator Scholarship from Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Breaking symmetry: effects of habitat disturbance on flight-related traits of two Triatominae species

IntroductiomHabitat fragmentation alters environmental structure and imposes selective pressures on dispersal-related traits in insect vectors, potentially driving morphological adaptations that enhance flight performance. In this study, weinvestigate how landscape metrics influence the size and shape of the head and wings in two Triatominae species, Triatoma garciabesi and T. guasayana, which present differing ecological strategies. We hypothesize that individuals from more fragmented landscapes exhibit phenotypic shifts associated with enhanced dispersal capacity and increased morphological symmetry.MethodsTo test this, we combined community-based sampling of triatomines with geometric morphometrics and multiscale landscape metrics. We applied geometric morphometrics and generalized linear models (GLM)-based analyses to assess the effects of habitat fragmentation on flight-related morphology.ResultsOur results reveal that T. garciabesi shows increased head asymmetry and narrower wings in highly fragmented landscapes, while T. guasayana exhibits subtle shifts in head shape asymmetry and greater sexual dimorphism. In both species, head and wing sizes tended to be larger in fragmented habitats, especially in females, suggesting differential morphological responses that may reflect species-specific dispersal strategies.DiscussionHabitat fragmentation differentially affects T. garciabesi and T. guasayana, leading to distinct dispersal syndromes. Triatoma garciabesi shows greater plasticity, highlighting the role of landscape structure in shaping adaptive dispersal traits

Data integration in the era of omics: current and future challenges

To integrate heterogeneous and large omics data constitutes not only a conceptual challenge but a practical hurdle in the daily analysis of omics data. With the rise of novel omics technologies and through large-scale consortia projects, biological systems are being further investigated at an unprecedented scale generating heterogeneous and often large data sets. These data-sets encourage researchers to develop novel data integration methodologies. In this introduction we review the definition and characterize current efforts on data integration in the life sciences. We have used a web-survey to assess current research projects on data-integration to tap into the views, needs and challenges as currently perceived by parts of the research community

Autores de la región misionera: las producciones literarias de los años noventa a la actualidad. 16H211

Este proyecto estuvo encaminado analizar los textos literarios y de la literatura misionera, editados desde 1990 hasta la actualidad. La investigación ha sido posible por la existencia de una numerosa producción literaria durante el período mencionado. En una primera etapa se incursionó en conceptos teóricos que hicieron inteligibles ciertas particularidades de los textos considerados. En primer término, hemos postulado un concepto de región construido desde la diferencia, con el que se intentó considerar los matices de heterogeneidad e hibridez, característicos de la población en estudio. Su aplicación permitió superar o mantener en suspenso el tratamiento de los estereotipos de la tradición así como los temas y los personajes propios del marco geográfico, para considerar, en cambio, las reglas de la práctica discursiva, así como los procedimientos empleados en la modelización de los textos. Uno de los criterios seguidos fue considerar el tiempo como factor a tener en cuenta en la constitución del corpus. Si bien, toda producción literaria no irrumpe repentinamente sino que se necesitan décadas para que ella adquiera vigencia en la mente de los lectores, lo cierto es que hemos inventariado más de cien obras de diferentes momentos de los 90 hasta la actualidad. La lectura arrojó una gran variación respecto de las manifestaciones literarias y las posiciones de los escritores de los años 80 y 70, descriptos en investigaciones anteriores como más apegados a la escritura individual y al rescate de los valores tradicionales, en el terreno literario. Otro criterio fue considerar en los lugares de producción periférica la presencia de una literatura menor con visibles marcas de desterritorialización. Fue preciso estudiar las relaciones discontinuas así como las relaciones de fuerza en las articulaciones o agenciamientos singulares de discursos de diversos espacios de modelización. En sentido general, durante el período analizado en esta investigación, la literatura de Misiones muestra una desordenada dispersión de sus recorridos, en ciertos aspectos despojada de los lugares comunes propios del canon tradicional. Esto hizo posible indagar en variadas producciones estéticas del imaginario local

EpiCass and CassavaNet4Dev advanced bioinformatics workshop

Open Access ArticleEpiCass and CassavaNet4Dev are collaborative projects funded by the Swedish Research Council between the Swedish University of Agriculture (SLU) and the International Institute of Tropical Agriculture (IITA). The projects aim to investigate the influence of epigenetic changes on agricultural traits such as yield and virus resistance while also providing African students and researchers with advanced bioinformatics training and opportunities to participate in big data analysis events. The first advanced bioinformatics training workshop took place from May 16th to May 18th, 2022, followed by an online mini-symposium titled "Epigenetics and crop improvement" on May 19th. The symposium featured international speakers covering a wide range of topics related to plant epigenetics, cassava viral diseases, and cassava breeding strategies. A new online and on-site teaching concept was developed for the three-day workshop to ensure maximum student participation across Western, Eastern, and Southern Africa. Initially planned in Nigeria, Kenya, Ethiopia, Tanzania, and Zambia, the workshop ultimately focused on Nigeria, Kenya, and Ethiopia due to a lack of qualified candidates in the other countries. Each classroom hosted 20 to 25 students, with at least one bioinformatician present for support. The classrooms were connected via video conferencing, whereas teachers located in different places in Africa and Europe joined the video stream to conduct teaching sessions. The workshop was divided into theoretical classes and hands-on sessions, where participants could run data analysis with support from online teachers and local bioinformaticians. To enable participants to run guided, CPU and RAM-intensive data analysis workflows and overcome local computing and internet access restrictions, a system of virtual machines (VMs) hosted in the cloud was developed. The teaching platform provided teaching and exercise materials to support the use of the VMs. Although some students could not run heavy data analysis workflows due to unforeseen restrictions in the cloud, these issues were solved. All participants had the opportunity to run the analysis steps independently in the cloud using the protocols hosted on the teaching platform

Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification

Numerous protocols have been published for extracting DNA from phlebotomines. Nevertheless, their small size is generally an issue in terms of yield, efficiency, and purity, for large-scale individual sand fly DNA extractions when using traditional methods. Even though this can be circumvented with commercial kits, these are generally cost-prohibitive for developing countries. We encountered these limitations when analyzing field-collected Lutzomyia spp. by polymerase chain reaction (PCR) and, for this reason, we evaluated various modifications on a previously published protocol, the most significant of which was a different lysis buffer that contained Ca2+ (buffer TESCa). This ion protects proteinase K against autolysis, increases its thermal stability, and could have a regulatory function for its substrate-binding site. Individual sand fly DNA extraction success was confirmed by amplification reactions using internal control primers that amplify a fragment of the cacophony gene. To the best of our knowledge, this is the first time a lysis buffer containing Ca2+ has been reported for the extraction of DNA from sand flies.Centro Regional de Estudios Genómico