Variability of the aminoacid racemization relation in a group of Quaternary continental gastropods in Murcia (Spain)

La aplicación de los resultados de un análisis de racemización de aminoácidos en geocronología ofrece importantes ventajas respecto a otras técnicas, al ser esta más rápida y económica. Sin embargo, como con cualquier otra técnica, la racemización no está exenta de fuentes de incertidumbres que deben ser analizadas previamente a la interpretación. En este trabajo se exponen los resultados obtenidos del análisis de racemización de un grupo de 25 gasterópodos continentales fósiles hallados en una excavación de investigación paleosismológica en el municipio de Alhama de Murcia. En particular se compara la ratio entre aminoácidos con estructura dextrógira y levógira (relación D/L) de los ácidos aspártico y glutámico. En primer lugar, se revisan las principales fuentes de incertidumbre que afectan a la racemización. Seguidamente se muestra la metodología empleada para descartar muestras y, finalmente, se aborda una interpretación preliminar de los resultados en relación con la estratigrafía local y la paleosismologíaThe use of the amino acid racemization technique in geochronology offers a great advantage compared to other techniques, as racemization analyses are usually much faster and cheaper. However, as in any other technique, amino acid racemization is subject to a number of uncertainties, which have to be thoroughly analysed previously to any interpretation of the data. In this work, we present the results obtained in a group of 25 continental gastropods found in a paleoseismological trench in Alhama de Murcia (Betic Range, Spain). We carry out the comparison of dextrorotatory and levorotatory configuration (relation D/L) of the aspartic and glutamic acids. First, we review the different sources of uncertainty in the racemization process. Subsequently we show the methodology followed for discarding samples and, finally, we interpret in preliminary terms the results in relation to the local stratigraphy and paleoseismolog

Microbial volatile emissions promote accumulation of exceptionally high levels of starch in leaves in mono- and di-cotyledonous plants

Trabajo presentado en la X Reunión de Biología Molecular de plantas, celebrada en Valencia del 8 al 10 de julio de 2010.Peer Reviewe

CAR density influences antitumoral efficacy of BCMA CAR T cells and correlates with clinical outcome

Identification of new markers associated with long-term efficacy in patients treated with CAR T cells is a current medical need, particularly in diseases such as multiple myeloma. In this study, we address the impact of CAR density on the functionality of BCMA CAR T cells. Functional and transcriptional studies demonstrate that CAR T cells with high expression of the CAR construct show an increased tonic signaling with up-regulation of exhaustion markers and increased in vitro cytotoxicity but a decrease in in vivo BM infiltration. Characterization of gene regulatory networks using scRNA-seq identified regulons associated to activation and exhaustion up-regulated in CARHigh T cells, providing mechanistic insights behind differential functionality of these cells. Last, we demonstrate that patients treated with CAR T cell products enriched in CARHigh T cells show a significantly worse clinical response in several hematological malignancies. In summary, our work demonstrates that CAR density plays an important role in CAR T activity with notable impact on clinical response

Clinical Study Clinical Safety and Immunogenicity of Tumor-Targeted, Plant-Made Id-KLH Conjugate Vaccines for Follicular Lymphoma

We report the first evaluation of plant-made conjugate vaccines for targeted treatment of B-cell follicular lymphoma (FL) in a Phase I safety and immunogenicity clinical study. Each recombinant personalized immunogen consisted of a tumor-derived, plantproduced idiotypic antibody (Ab) hybrid comprising the hypervariable regions of the tumor-associated light and heavy Ab chains, genetically grafted onto a common human IgG1 scaffold. Each immunogen was produced in Nicotiana benthamiana plants using twin magnICON vectors expressing the light and heavy chains of the idiotypic Ab. Each purified Ab was chemically linked to the carrier protein keyhole limpet hemocyanin (KLH) to form a conjugate vaccine. The vaccines were administered to FL patients over a series of ≥6 subcutaneous injections in conjunction with the adjuvant Leukine (GM-CSF). The 27 patients enrolled in the study had previously received non-anti-CD20 cytoreductive therapy followed by ≥4 months of immune recovery prior to first vaccination. Of 11 patients who became evaluable at study conclusion, 82% (9/11) displayed a vaccine-induced, idiotype-specific cellular and/or humoral immune response. No patients showed serious adverse events (SAE) related to vaccination. The fully scalable plant-based manufacturing process yields safe and immunogenic personalized FL vaccines that can be produced within weeks of obtaining patient biopsies

Consensus document of the Spanish Society of Intensive and Critical Care Medicine and Coronary Units (SEMICYUC), the Spanish Society of Otorhinolaryngology and Head and Neck Surgery (SEORL-CCC) and the Spanish Society of Anesthesiology and Resuscitation (SEDAR) on tracheotomy in patients with COVID-19 infection.

La alta incidencia de insuficiencia respiratoria aguda en el contexto de la pandemia por COVID-19ha conllevado el uso de ventilación mecánica hasta en un 15%. Dado que la traqueotomía es un procedimiento quirúrgico frecuente, este documento de consenso, elaborado por tres Sociedades Científicas, la SEMICYUC, la SEDAR y la SEORL-CCC, tiene como objetivo ofrecer una revisión de las indicaciones y contraindicaciones de traqueotomía, ya sea por punción o abierta, esclarecer las posibles ventajas y exponer las condiciones ideales en que deben realizarse y los pasos que considerar en su ejecución. Se abordan situaciones regladas y urgentes, así como los cuidados postoperatorios.post-print393 K

Global wealth disparities drive adherence to COVID-safe pathways in head and neck cancer surgery

Peer reviewe

Variability of the aminoacid racemization relation in a group of Quaternary continental gastropods in Murcia (Spain)

La aplicación de los resultados de un análisis de racemización de aminoácidos en geocronología ofrece importantes ventajas respecto a otras técnicas, al ser esta más rápida y económica. Sin embargo, como con cualquier otra técnica, la racemización no está exenta de fuentes de incertidumbres que deben ser analizadas previamente a la interpretación. En este trabajo se exponen los resultados obtenidos del análisis de racemización de un grupo de 25 gasterópodos continentales fósiles hallados en una excavación de investigación paleosismológica en el municipio de Alhama de Murcia. En particular se compara la ratio entre aminoácidos con estructura dextrógira y levógira (relación D/L) de los ácidos aspártico y glutámico. En primer lugar, se revisan las principales fuentes de incertidumbre que afectan a la racemización. Seguidamente se muestra la metodología empleada para descartar muestras y, finalmente, se aborda una interpretación preliminar de los resultados en relación con la estratigrafía local y la paleosismología.The use of the amino acid racemization technique in geochronology offers a great advantage compared to other techniques, as racemization analyses are usually much faster and cheaper. However, as in any other technique, amino acid racemization is subject to a number of uncertainties, which have to be thoroughly analysed previously to any interpretation of the data. In this work, we present. the results obtained in a group of 25 continental gastropods found in a paleoseismological trench in Alhama de Murcia (Betic Range, Spain). We carry out the comparison of dextrorotatory and levorotatory configuration (relation D/L) of the aspartic and glutamic acids. First, we review the different sources of uncertainty in the racemization process. Subsequently we show the methodology followed for discarding samples and, finally, we interpret in preliminary terms the results in relation to the local stratigraphy and paleoseismologyMinisterio de Ciencia e Innovación (MICINN)Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEpu

Consistent cortical responses from subcorticaly delivered electrical stimuli:A study oriented to visual prostheses

International Conference on Health Informatics, HEALTHINF 2009 (2. 2009. Porto