77 research outputs found
Anti-ebola virus antibody levels in convalescent plasma and viral load after plasma infusion in patients with ebola virus disease
Background Ebola virus (EBOV) neutralizing antibody in plasma may reduce viral load following administration of plasma to patients with Ebola virus disease (EVD), but measurement of these antibodies is complex. Methods Anti-EBOV antibody was measured by 2 neutralization and 2 enzyme-linked immunosorbent assays (ELISAs) in convalescent plasma (ECP) from 100 EVD survivor donors in Liberia. Viral load was assessed repetitively in patients with EVD participating in a clinical trial of enhanced standard of care plus ECP. Results All 4 anti-EBOV assays were highly concordant for detection of EBOV antibody. Antibodies were not detected in plasma specimens obtained from 15 of 100 donors, including 7 with documented EBOV-positive reverse-transcription polymerase chain reaction during EVD. Viral load was reduced following each dose in the 2 clinical trial participants who received ECP with higher antibody levels but not in the 2 who received ECP with lower antibody levels. Conclusions Recovery from EVD can occur with absence of detectable anti-EBOV antibody several months after disease onset. ELISAs may be useful to select ECP donors or identify ECP units that contain neutralizing antibody. ECP with higher anti-EBOV antibody levels may have greater effect on EBOV load - an observation that requires further investigation. Clinical Trials Registration NCT02333578
Apheresis for collection of Ebola convalescent plasma in Liberia
Purpose: This report describes initiation of apheresis capability in Liberia, Africa to support a clinical trial of convalescent plasma therapy for Ebola Virus Disease. Methods: A bloodmobile was outfitted in the United States as a four-bed apheresis unit with capabilities including pathogen reduction, electronic blood establishment computer system, designated areas for donor counseling and laboratory testing, and onboard electrical power generation. After air transport to Liberia, the bloodmobile was positioned at ELWA Hospital, Monrovia, and connected to the hospital's power grid. Liberian staff were trained to conduct donor screening, which included questionnaire and onsite blood typing and transfusion transmitted infection (TTI) testing, and plasma collection and processing. Results: The bloodmobile was operational within 3 weeks after arrival of the advance team. Of 101 donors who passed the pre-screening questionnaire, 32 were deferred. Twenty-eight of ninty-nine tested survivors were deferred for positive transfusion transmitted infection (TTI) tests; twenty-one were positive for hepatitis B, hepatitis C, or human immunodeficiency virus. The majority of donors had type O blood; all but one were Rh positive. Forty-three survivors donated at least once; eighty-nine apheresis attempts resulted in eighty-one successful collections. Conclusions: Apheresis capability was emergently established in Liberia to support an efficacy trial of Ebola Convalescent Plasma. Extensive cooperation among multinational team members, engineers, logisticians, and blood safety technical personnel at the operational site was required to surmount challenges to execution posed by logistical factors. The high proportion of positive TTI tests supported the use of a pathogen reduction system to enhance product safety
Cosmological distance indicators
We review three distance measurement techniques beyond the local universe:
(1) gravitational lens time delays, (2) baryon acoustic oscillation (BAO), and
(3) HI intensity mapping. We describe the principles and theory behind each
method, the ingredients needed for measuring such distances, the current
observational results, and future prospects. Time delays from strongly lensed
quasars currently provide constraints on with < 4% uncertainty, and with
1% within reach from ongoing surveys and efforts. Recent exciting discoveries
of strongly lensed supernovae hold great promise for time-delay cosmography.
BAO features have been detected in redshift surveys up to z <~ 0.8 with
galaxies and z ~ 2 with Ly- forest, providing precise distance
measurements and with < 2% uncertainty in flat CDM. Future BAO
surveys will probe the distance scale with percent-level precision. HI
intensity mapping has great potential to map BAO distances at z ~ 0.8 and
beyond with precisions of a few percent. The next years ahead will be exciting
as various cosmological probes reach 1% uncertainty in determining , to
assess the current tension in measurements that could indicate new
physics.Comment: Review article accepted for publication in Space Science Reviews
(Springer), 45 pages, 10 figures. Chapter of a special collection resulting
from the May 2016 ISSI-BJ workshop on Astronomical Distance Determination in
the Space Ag
Fitting the integrated Spectral Energy Distributions of Galaxies
Fitting the spectral energy distributions (SEDs) of galaxies is an almost
universally used technique that has matured significantly in the last decade.
Model predictions and fitting procedures have improved significantly over this
time, attempting to keep up with the vastly increased volume and quality of
available data. We review here the field of SED fitting, describing the
modelling of ultraviolet to infrared galaxy SEDs, the creation of
multiwavelength data sets, and the methods used to fit model SEDs to observed
galaxy data sets. We touch upon the achievements and challenges in the major
ingredients of SED fitting, with a special emphasis on describing the interplay
between the quality of the available data, the quality of the available models,
and the best fitting technique to use in order to obtain a realistic
measurement as well as realistic uncertainties. We conclude that SED fitting
can be used effectively to derive a range of physical properties of galaxies,
such as redshift, stellar masses, star formation rates, dust masses, and
metallicities, with care taken not to over-interpret the available data. Yet
there still exist many issues such as estimating the age of the oldest stars in
a galaxy, finer details ofdust properties and dust-star geometry, and the
influences of poorly understood, luminous stellar types and phases. The
challenge for the coming years will be to improve both the models and the
observational data sets to resolve these uncertainties. The present review will
be made available on an interactive, moderated web page (sedfitting.org), where
the community can access and change the text. The intention is to expand the
text and keep it up to date over the coming years.Comment: 54 pages, 26 figures, Accepted for publication in Astrophysics &
Space Scienc
Reproduction of Leishmania (Leishmania ) infantum chagasi in conditioned cell culture growth medium
Characterisation of <i>Herschel</i>-selected strong lens candidates through <i>HST</i> and sub-mm/mm observations
We have carried out HST snapshot observations at 1.1 ÎŒm of 281 candidate strongly lensed galaxies identified in the wide-area extragalactic surveys conducted with the Herschel space observatory. Our candidates comprise systems with flux densities at 500 ÎŒm S500 â„ 80 mJy. We model and subtract the surface brightness distribution for 130 systems, where we identify a candidate for the foreground lens candidate. After combining visual inspection, archival high-resolution observations, and lens subtraction, we divide the systems into different classes according to their lensing likelihood. We confirm 65 systems to be lensed. Of these, 30 are new discoveries. We successfully perform lens modelling and source reconstruction on 23 systems, where the foreground lenses are isolated galaxies and the background sources are detected in the HST images. All the systems are successfully modelled as a singular isothermal ellipsoid. The Einstein radii of the lenses and the magnifications of the background sources are consistent with previous studies. However, the background source circularised radii (between 0.34 kpc and 1.30 kpc) are âŒ3 times smaller than the ones measured in the sub-mm/mm for a similarly selected and partially overlapping sample. We compare our lenses with those in the SLACS survey, confirming that our lens-independent selection is more effective at picking up fainter and diffuse galaxies and group lenses. This sample represents the first step towards characterising the near-IR properties and stellar masses of the gravitationally lensed dusty star-forming galaxies
A dusty proto-cluster surrounding the binary galaxy HerBS-70 at z = 2.3
We report on deep SCUBA-2 observations at 850 ÎŒm and NOEMA spectroscopic measurements at 2 mm of the environment surrounding the luminous, massive (M* â 2 Ă 1011 Mâ) Herschel-selected source HerBS-70. This source was revealed by previous NOEMA observations to be a binary system of dusty star-forming galaxies at z = 2.3, with the East component (HerBS-70E) hosting an Active Galactic Nucleus (AGN). The SCUBA-2 observations detected, in addition to the binary system, twenty-one sources at >3.5Ï over an area of âŒ25 square comoving Mpc with a sensitivity of 1Ï850 = 0.75 mJy. The surface density of continuum sources around HerBS-70 is three times higher than for field galaxies. The NOEMA spectroscopic measurements confirm the protocluster membership of three of the nine brightest sources through their CO(4â3) line emission, yielding a volume density 36 times higher than for field galaxies. All five confirmed sub-mm galaxies in the HerBS-70 system have relatively short gas depletion times (80 â 500 Myr), indicating the onset of quenching for this protocluster core due to the depletion of gas. The dark matter halo mass of the HerBS-70 system is estimated around 5 Ă 1013 Mâ, with a projected current-day mass of 1015 Mâ, similar to the local Virgo and Coma clusters. These observations support the claim that DSFGs, in particular the ones with observed multiplicity, can trace cosmic overdensities
Long-Baseline Neutrino Facility (LBNF) and Deep Underground Neutrino Experiment (DUNE) Conceptual Design Report Volume 2: The Physics Program for DUNE at LBNF
The Physics Program for the Deep Underground Neutrino Experiment (DUNE) at the Fermilab Long-Baseline Neutrino Facility (LBNF) is described
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