Interleukin-10 Production by T and B Cells Is a Key Factor to Promote Systemic Salmonella enterica Serovar Typhimurium Infection in Mice

Indexción: Scopus.Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative bacterium that produces disease in numerous hosts. In mice, oral inoculation is followed by intestinal colonization and subsequent systemic dissemination, which leads to severe pathogenesis without the activation of an efficient anti-Salmonella immune response. This feature suggests that the infection caused by S. Typhimurium may promote the production of anti-inflammatory molecules by the host that prevent efficient T cell activation and bacterial clearance. In this study, we describe the contribution of immune cells producing the anti-inflammatory cytokine interleukin-10 (IL-10) to the systemic infection caused by S. Typhimurium in mice. We observed that the production of IL-10 was required by S. Typhimurium to cause a systemic disease, since mice lacking IL-10 (IL-10-/-) were significantly more resistant to die after an infection as compared to wild-type (WT) mice. IL-10-/- mice had reduced bacterial loads in internal organs and increased levels of pro-inflammatory cytokines in serum at 5 days of infection. Importantly, WT mice showed high bacterial loads in tissues and no increase of cytokines in serum after 5 days of S. Typhimurium infection, except for IL-10. In WT mice, we observed a peak of il-10 messenger RNA production in ileum, spleen, and liver after 5 days of infection. Importantly, the adoptive transfer of T or B cells from WT mice restored the susceptibility of IL-10-/- mice to systemic S. Typhimurium infection, suggesting that the generation of regulatory cells in vivo is required to sustain a systemic infection by S. Typhimurium. These findings support the notion that IL-10 production from lymphoid cells is a key process in the infective cycle of S. Typhimurium in mice due to generation of a tolerogenic immune response that prevents bacterial clearance and supports systemic dissemination

Relación entre la sedación profunda y la aparición de delirium en el postoperatorio de pacientes ancianos sometidos a una intervención quirúrgica por fractura de cadera

Objetivo Se llevó a cabo una revisión sistemática para evaluar y caracterizar la relación entre la presentación de delirium postoperatorio y el tipo de anestesia en pacientes ancianos con fractura de cadera.Adulto mayo

Régimen alimentario de siete especies ícticas en el embalse de la hidroeléctrica Urrá (Córdoba, Colombia)

El objeto del estudio fue evaluar el régimen alimentario de siete especies de peces que se han establecido en el embalse de la Hidroeléctrica Urrá (HU). Se analizaron 453 estómagos, distribuidos así: 115 de perico (Tracheolypterus badeli cf), 111 de cacucho (Panaque gibbosus), 108 de yalúa (Cyphocharax magdalenae), 89 de mojarra amarilla (Caquetaia kraussii) , 11 de moncholo (Hoplias malabaricus), 10 de barbul ( Pimelodus clarias) y 9 de doncella ( Ageneoisus pardales) . Los contenidos estomacales se evaluaron mediante frecuencia de ocurrencia (FO), frecuencia numérica (FN) y el método gravimétrico (G). Se determinó la importancia de cada presa mediante el índice de categorización de presa (ICP) y la superposición de dietas se analizó mediante el Índice de Morosita (IM). La distribución porcentual de los contenidos estomacales de la yalúa mostró que el 95.5% correspondió al ítem detritus y el restante 4.5% a microlagas (Clorofitas y Bacillariofitas) y protozoarios/rotíferos. La dieta del cacucho la conformaron en un 70.9% microalgas (Cianofitas, Clorofitas y Bacilariofitas) y protozoarios/rotíferos y el restante 29.1% correspondieron al ítem detritus. Barbul (FO = 50%, FN = 46.2%) y perico se alimentaron principalmente de insectos (FO = 73.9%, FN = 86.4%). La mojarra amarilla (FO = 87.1%, FN = 83.3%), doncella (FO = 92.3%, FN = 92.0%) y moncholo (FO = 90.9%, FN = 90.9%) prefieren peces como cachanita ( Roeboides dayi ) y sardinas ( Astianax sp). Los resultados sugieren que yalúa es detritívoro iliófago, cacucho es detritívoro, perico y barbul son carnívoros insectívoros y doncella, moncholo y mojarra amarilla son carnívoros piscívoros

Deleción terminal del brazo largo del cromosoma 9 en leucemia promielocítica aguda

La Leucemia Promielocítica Aguda (LPA), definida como M3 en la clasificación francesa-americana- británica (FAB)(1), se caracteriza por presentar una translocación cromosómica al azar t(15;17)(q22;q21). En esta translocación se fusionan los receptores del ácido retinoíco ?(? RAR) gen localizado en el cromosoma 17 y el gen PML sobre el cromosoma 15(2). Por otra lado, la deleción del brazo largo del cromosoma 9(9q-) es una alteración rara específica encontrada en (LMA) como una anormalidad única del cariotipo o como un cambio secundario, particularmente junto con t(8;21)(q22;q22)(3,4). La mayoría de ellas son deleciones intersticiales, 9q12 y 9q22; constituyen el sitio más común de ruptura proximal y distal (5)

Commonality and variation in mental representations of music revealed by a cross-cultural comparison of rhythm priors in 15 countries

Music is present in every known society but varies from place to place. What, if anything, is universal to music cognition? We measured a signature of mental representations of rhythm in 39 participant groups in 15 countries, spanning urban societies and Indigenous populations. Listeners reproduced random 'seed' rhythms; their reproductions were fed back as the stimulus (as in the game of 'telephone'), such that their biases (the prior) could be estimated from the distribution of reproductions. Every tested group showed a sparse prior with peaks at integer-ratio rhythms. However, the importance of different integer ratios varied across groups, often reflecting local musical practices. Our results suggest a common feature of music cognition: discrete rhythm 'categories' at small-integer ratios. These discrete representations plausibly stabilize musical systems in the face of cultural transmission but interact with culture-specific traditions to yield the diversity that is evident when mental representations are probed across many cultures. [Abstract copyright: © 2024. The Author(s).

VOLUMEN 15, NÚMERO 29 (1981-1982)

USO DEL COMPUTADOR EN EL ANÁLISIS SEDIMENTOLÓGICO Y DE MIGRACIÓN DE FLUIDOS Y SU APLICACIÓN EN EL NOROCCIDENTE COLOMBIANO. Castro, A. E.; Molina, G. J.UN MODELO ESTRUCTURAL PARA EL VALLE DEL CAUCA Y SUS IMPLICACIONES HIDROGEOLÓGICAS Y MINERAS. Padilla, L. E.TECTÓNICA DE SOBRECORRIMIENTO Y SUS IMPLICACIONES ESTRUCTURALES EN EL ÁREA PAMPLONA - CORDILLERA ORIENTAL DE COLOMBIA. Boinet, T.; Bourgois, J.; Mendoza F. HGEOLOGÍA DE LAS LATERITAS NIQUELÍFERAS DE CERRO MATOSO S. A. Mejía, V. M.; Durango, J. R.COMPLEJO OFIOLÍTICO EN LA CUENCA DEL RÍO GUAPI. Ortega Montero, C.DETERMINACIÓN DE RESERVAS DE DEPÓSITOS MINERALES. Hernández Garay, H.CARACTERIZACIÓN, RANGO Y CLASIFICACIÓN DE CARBONES. Ramírez Castro, P

VOLUMEN 15, NÚMERO 29 (1981-1982)

USO DEL COMPUTADOR EN EL ANÁLISIS SEDIMENTOLÓGICO Y DE MIGRACIÓN DE FLUIDOS Y SU APLICACIÓN EN EL NOROCCIDENTE COLOMBIANO. Castro, A. E.; Molina, G. J.UN MODELO ESTRUCTURAL PARA EL VALLE DEL CAUCA Y SUS IMPLICACIONES HIDROGEOLÓGICAS Y MINERAS. Padilla, L. E.TECTÓNICA DE SOBRECORRIMIENTO Y SUS IMPLICACIONES ESTRUCTURALES EN EL ÁREA PAMPLONA - CORDILLERA ORIENTAL DE COLOMBIA. Boinet, T.; Bourgois, J.; Mendoza F. HGEOLOGÍA DE LAS LATERITAS NIQUELÍFERAS DE CERRO MATOSO S. A. Mejía, V. M.; Durango, J. R.COMPLEJO OFIOLÍTICO EN LA CUENCA DEL RÍO GUAPI. Ortega Montero, C.DETERMINACIÓN DE RESERVAS DE DEPÓSITOS MINERALES. Hernández Garay, H.CARACTERIZACIÓN, RANGO Y CLASIFICACIÓN DE CARBONES. Ramírez Castro, P

The Impact of CpG Island on Defining Transcriptional Activation of the Mouse L1 Retrotransposable Elements

BACKGROUND: L1 retrotransposable elements are potent insertional mutagens responsible for the generation of genomic variation and diversification of mammalian genomes, but reliable estimates of the numbers of actively transposing L1 elements are mostly nonexistent. While the human and mouse genomes contain comparable numbers of L1 elements, several phylogenetic and L1Xplore analyses in the mouse genome suggest that 1,500-3,000 active L1 elements currently exist and that they are still expanding in the genome. Conversely, the human genome contains only 150 active L1 elements. In addition, there is a discrepancy among the nature and number of mouse L1 elements in L1Xplore and the mouse genome browser at the UCSC and in the literature. To date, the reason why a high copy number of active L1 elements exist in the mouse genome but not in the human genome is unknown, as are the potential mechanisms that are responsible for transcriptional activation of mouse L1 elements. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the promoter sequences of the 1,501 potentially active mouse L1 elements retrieved from the GenBank and L1Xplore databases and evaluated their transcription factors binding sites and CpG content. To this end, we found that a substantial number of mouse L1 elements contain altered transcription factor YY1 binding sites on their promoter sequences that are required for transcriptional initiation, suggesting that only a half of L1 elements are capable of being transcriptionally active. Furthermore, we present experimental evidence that previously unreported CpG islands exist in the promoters of the most active T(F) family of mouse L1 elements. The presence of sequence variations and polymorphisms in CpG islands of L1 promoters that arise from transition mutations indicates that CpG methylation could play a significant role in determining the activity of L1 elements in the mouse genome. CONCLUSIONS: A comprehensive analysis of mouse L1 promoters suggests that the number of transcriptionally active elements is significantly lower than the total number of full-length copies from the three active mouse L1 families. Like human L1 elements, the CpG islands and potentially the transcription factor YY1 binding sites are likely to be required for transcriptional initiation of mouse L1 elements