249 research outputs found
Axion searches with the EDELWEISS-II experiment
We present new constraints on the couplings of axions and more generic
axion-like particles using data from the EDELWEISS-II experiment. The EDELWEISS
experiment, located at the Underground Laboratory of Modane, primarily aims at
the direct detection of WIMPs using germanium bolometers. It is also sensitive
to the low-energy electron recoils that would be induced by solar or dark
matter axions. Using a total exposure of up to 448 kg.d, we searched for
axion-induced electron recoils down to 2.5 keV within four scenarios involving
different hypotheses on the origin and couplings of axions. We set a 95% CL
limit on the coupling to photons GeV in
a mass range not fully covered by axion helioscopes. We also constrain the
coupling to electrons, , similar to the more
indirect solar neutrino bound. Finally we place a limit on , where is the
effective axion-nucleon coupling for Fe. Combining these results we
fully exclude the mass range keV for DFSZ axions and
keV for KSVZ axions
A search for low-mass WIMPs with EDELWEISS-II heat-and-ionization detectors
We report on a search for low-energy (E < 20 keV) WIMP-induced nuclear
recoils using data collected in 2009 - 2010 by EDELWEISS from four germanium
detectors equipped with thermal sensors and an electrode design (ID) which
allows to efficiently reject several sources of background. The data indicate
no evidence for an exponential distribution of low-energy nuclear recoils that
could be attributed to WIMP elastic scattering after an exposure of 113 kg.d.
For WIMPs of mass 10 GeV, the observation of one event in the WIMP search
region results in a 90% CL limit of 1.0x10^-5 pb on the spin-independent
WIMP-nucleon scattering cross-section, which constrains the parameter space
associated with the findings reported by the CoGeNT, DAMA and CRESST
experiments.Comment: PRD rapid communication accepte
Proceedings of the third French-Ukrainian workshop on the instrumentation developments for HEP
The reports collected in these proceedings have been presented in the third
French-Ukrainian workshop on the instrumentation developments for high-energy
physics held at LAL, Orsay on October 15-16. The workshop was conducted in the
scope of the IDEATE International Associated Laboratory (LIA). Joint
developments between French and Ukrainian laboratories and universities as well
as new proposals have been discussed. The main topics of the papers presented
in the Proceedings are developments for accelerator and beam monitoring,
detector developments, joint developments for large-scale high-energy and
astroparticle physics projects, medical applications.Comment: 3rd French-Ukrainian workshop on the instrumentation developments for
High Energy Physics, October 15-16, 2015, LAL, Orsay, France, 94 page
Purification of molybdenum oxide, growth and characterization of medium size zinc molybdate crystals for the LUMINEU program
The LUMINEU program aims at performing a pilot experiment on neutrinoless
double beta decay of 100Mo using radiopure ZnMoO4 crystals operated as
scintillating bolometers. Growth of high quality radiopure crystals is a
complex task, since there are no commercially available molybdenum compounds
with the required levels of purity and radioactive contamination. This paper
discusses approaches to purify molybdenum and synthesize compound for high
quality radiopure ZnMoO4 crystal growth. A combination of a double sublimation
(with addition of zinc molybdate) with subsequent recrystallization in aqueous
solutions (using zinc molybdate as a collector) was used. Zinc molybdate
crystals up to 1.5 kg were grown by the low-thermal-gradient Czochralski
technique, their optical, luminescent, diamagnetic, thermal and bolometric
properties were tested.Comment: Contribution to Proc. of Int. Workshop on Radiopure Scintillators
RPSCINT 2013, 17-20 September 2013, Kyiv, Ukraine; to be published in EPJ Web
of Conferences; expected to be online in January 2014; 6 pages, 6 figures,
and 3 table
Scintillating bolometers based on ZnMoO4 and Zn100MoO4 crystals to search for 0ν2β decay of 100Mo (LUMINEU project): first tests at the Modane Underground Laboratory
The technology of scintillating bolometers based on zinc molybdate (ZnMoO4) crystals is under development within the LUMINEU project to search for decay of 100Mo with the goal to set the basis for large scale experiments capable to explore the inverted hierarchy region of the neutrino mass pattern. Advanced ZnMoO4 crystal scintillators with mass of ∼0.3 kg were developed and Zn100MoO4 crystal from enriched 100Mo was produced for the first time by using the low-thermal-gradient Czochralski technique. One ZnMoO4 scintillator and two samples (59 g and 63 g) cut from the enriched boule were tested aboveground at milli-Kelvin temperature as scintillating bolometers showing a high detection performance. The first results of the low background measurements with three ZnMoO4 and two enriched detectors installed in the EDELWEISS set-up at the Modane Underground Laboratory (France) are presented
Modified Vaccinia Virus Ankara-Based Vaccine Vectors Induce Apoptosis in Dendritic Cells Draining from the Skin via both the Extrinsic and Intrinsic Caspase Pathways, Preventing Efficient Antigen Presentation
Dendritic cells (DC) are potent antigen-presenting cells and central to the induction of immune responses following infection or vaccination. The collection of DC migrating from peripheral tissues by cannulation of the afferent lymphatic vessels provides DC which can be used directly ex vivo without extensive in vitro manipulations. We have previously used bovine migrating DC to show that recombinant human adenovirus 5 vectors efficiently transduce afferent lymph migrating DEC-205(+) CD11c(+) CD8(-) DC (ALDC). We have also shown that recombinant modified vaccinia virus Ankara (MVA) infects ALDC in vitro, causing downregulation of costimulatory molecules, apoptosis, and cell death. We now show that in the bovine system, modified vaccinia virus Ankara-induced apoptosis in DC draining from the skin occurs soon after virus binding via the caspase 8 pathway and is not associated with viral gene expression. We also show that after virus entry, the caspase 9 pathway cascade is initiated. The magnitude of T cell responses to mycobacterial antigen 85A (Ag85A) expressed by recombinant MVA-infected ALDC is increased by blocking caspase-induced apoptosis. Apoptotic bodies generated by recombinant MVA (rMVA)-Ag85A-infected ALDC and containing Ag85A were phagocytosed by noninfected migrating ALDC expressing SIRPα via actin-dependent phagocytosis, and these ALDC in turn presented antigen. However, the addition of fresh ALDC to MVA-infected cultures did not improve on the magnitude of the T cell responses; in contrast, these noninfected DC showed downregulation of major histocompatibility complex class II (MHC-II), CD40, CD80, and CD86. We also observed that MVA-infected ALDC promoted migration of DEC-205(+) SIRPα(+) CD21(+) DC as well as CD4(+) and CD8(+) T cells independently of caspase activation. These in vitro studies show that induction of apoptosis in DC by MVA vectors is detrimental to the subsequent induction of T cell responses
Innate Immune Response of Human Plasmacytoid Dendritic Cells to Poxvirus Infection Is Subverted by Vaccinia E3 via Its Z-DNA/RNA Binding Domain
Plasmacytoid dendritic cells (pDCs) play important roles in antiviral innate immunity by producing type I interferon (IFN). In this study, we assess the immune responses of primary human pDCs to two poxviruses, vaccinia and myxoma virus. Vaccinia, an orthopoxvirus, was used for immunization against smallpox, a contagious human disease with high mortality. Myxoma virus, a Leporipoxvirus, causes lethal disease in rabbits, but is non-pathogenic in humans. We report that myxoma virus infection of human pDCs induces IFN-α and TNF production, whereas vaccinia infection does not. Co-infection of pDCs with myxoma virus plus vaccinia blocks myxoma induction effects. We find that heat-inactivated vaccinia (Heat-VAC; by incubating the virus at 55°C for 1 h) gains the ability to induce IFN-α and TNF in primary human pDCs. Induction of IFN-α in pDCs by myxoma virus or Heat-VAC is blocked by chloroquine, which inhibits endosomal acidification required for TLR7/9 signaling, and by inhibitors of cellular kinases PI3K and Akt. Using purified pDCs from genetic knockout mice, we demonstrate that Heat-VAC-induced type I IFN production in pDCs requires the endosomal RNA sensor TLR7 and its adaptor MyD88, transcription factor IRF7 and the type I IFN feedback loop mediated by IFNAR1. These results indicate that (i) vaccinia virus, but not myxoma virus, expresses inhibitor(s) of the poxvirus sensing pathway(s) in pDCs; and (ii) Heat-VAC infection fails to produce inhibitor(s) but rather produces novel activator(s), likely viral RNA transcripts that are sensed by the TLR7/MyD88 pathway. Using vaccinia gene deletion mutants, we show that the Z-DNA/RNA binding domain at the N-terminus of the vaccinia immunomodulatory E3 protein is an antagonist of the innate immune response of human pDCs to poxvirus infection and TLR agonists. The myxoma virus ortholog of vaccinia E3 (M029) lacks the N-terminal Z-DNA/RNA binding domain, which might contribute to the immunostimulating properties of myxoma virus
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