A statistical comparison of the blood sedimentation rate and white blood cell count in acute respiratory conditions: with special reference to lobar pneumonia

The Sedimentation Rate in Lobar Pneumonia:1. The sedimentation rate is greatly increased throughout the febrile stage.2. The sedimentation rate tends to vary with the white blood cell count, inversely till about the sixth day, and directly thereafter.3. Body temperature exercises no influence on the sedimentation rate or white blood cell count.4. The sedimentation rate is of help in diagnosis.5. The sedimentation rate is of great value in the assessment of complications, especially of delayed resolution.6. Ante mortem retardation of the sedimentation rate invariably occurs

StrathE2E2 : an R package for modelling the dynamics of marine food webs and fisheries

1. We present StrathE2E2, an R package for modelling the whole ecosystem, or ‘big-picture’ effects of hydrodynamics, temperature, nutrient additions, and fishing on continental shelf marine food webs. 2. StrathE2E2 has two linked parts – a fishing fleet model and an ecology model. The fishing model combines harvesting, discarding and seabed disturbance rates across a range of gears and passes the results into the ecology model. 3. The ecology model is a network of coupled ordinary differential equations representing the rates of change in nitrogen mass of organic detritus, dissolved inorganic nutrient, and coarse guilds of living biomass spanning microbes to megafauna. The equations include representations of feeding, metabolism, reproduction, active migrations, advection and mixing. Environmental driving data include temperature, irradiance, hydrodynamics, and nutrient inputs from rivers, atmosphere and ocean boundaries. 4. The package includes functions for parameter optimization, global sensitivity analysis, and Monte Carlo estimation of credible intervals for model outputs 5. A fully developed and documented implementation for the North Sea is included with the package

Upper Mantle Structure of Central and West Antarctica from Array Analysis of Rayleigh Wave Phase Velocities

The seismic velocity structure of Antarctica is important, both as a constraint on the tectonic history of the continent and for understanding solid Earth interactions with the ice sheet. We use Rayleigh wave array analysis methods applied to teleseismic data from recent temporary broadband seismograph deployments to image the upper mantle structure of central and West Antarctica. Phase velocity maps are determined using a two–plane wave tomography method and are inverted for shear velocity using a Monte Carlo approach to estimate three-dimensional velocity structure. Results illuminate the structural dichotomy between the East Antarctic Craton and West Antarctica, with West Antarctica showing thinner crust and slower upper mantle velocity. West Antarctica is characterized by a 70–100 km thick lithosphere, underlain by a low-velocity zone to depths of at least 200 km. The slowest anomalies are beneath Ross Island and the Marie Byrd Land dome and are interpreted as upper mantle thermal anomalies possibly due to mantle plumes. The central Transantarctic Mountains are marked by an uppermost mantle slow-velocity anomaly, suggesting that the topography is thermally supported. The presence of thin, higher-velocity lithosphere to depths of about 70 km beneath the West Antarctic Rift System limits estimates of the regionally averaged heat flow to less than 90 mW/m2. The Ellsworth-Whitmore block is underlain by mantle with velocities that are intermediate between those of the West Antarctic Rift System and the East Antarctic Craton. We interpret this province as Precambrian continental lithosphere that has been altered by Phanerozoic tectonic and magmatic activity

Upper Mantle Seismic Anisotropy Beneath the West Antarctic Rift System and Surrounding Region from Shear Wave Splitting Analysis

We constrain azimuthal anisotropy in the West Antarctic upper mantle using shear wave splitting parameters obtained from teleseismic SKS, SKKS and PKS phases recorded at 37 broad-band seismometres deployed by the POLENET/ANET project. We use an eigenvalue technique to linearize the rotated and shifted shear wave horizontal particle motions and determine the fast direction and delay time for each arrival. High-quality measurements are stacked to determine the best fitting splitting parameters for each station. Overall, fast anisotropic directions are oriented at large angles to the direction of Antarctic absolute plate motion in both hotspot and no-net-rotation frameworks, showing that the anisotropy does not result from shear due to plate motion over the mantle. Further, the West Antarctic directions are substantially different from those of East Antarctica, indicating that anisotropy across the continent reflects multiple mantle regimes. We suggest that the observed anisotropy along the central Transantarctic Mountains (TAM) and adjacent West Antarctic Rift System (WARS), one of the largest zones of extended continental crust on Earth, results from asthenospheric mantle strain associated with the final pulse of western WARS extension in the late Miocene. Strong and consistent anisotropy throughout the WARS indicate fast axes subparallel to the inferred extension direction, a result unlike reports from the East African rift system and rifts within the Basin and Range, which show much greater variation. We contend that ductile shearing rather than magmatic intrusion may have been the controlling mechanism for accumulation and retention of such coherent, widespread anisotropic fabric. Splitting beneath the Marie Byrd Land Dome (MBL) is weaker than that observed elsewhere within the WARS, but shows a consistent fast direction, possibly representative of anisotropy that has been ‘frozen-in’ to remnant thicker lithosphere. Fast directions observed inland from the Amundsen Sea appear to be radial to the dome and may indicate radial horizontal mantle flow associated with an MBL plume head and low upper mantle velocities in this region, or alternatively to lithospheric features associated with the complex Cenozoic tectonics at the far-eastern end of the WARS

Conquest and Form: Narrativity in Joshua 5-11 and Historical Discourse in Ancient Judah

One goal of this essay is to offer an exploratory, historiographical analysis of the conquest account in the book of Joshua, an analysis that focuses upon the sociocultural milieu of ancient Judah. I propose to show how this narrative of conquest might have contributed to discourse(s) among the literate Judean community that perpetuated the text, and I will offer a few thoughts on the potential relationship between the narrative and the supposed cultic reforms of the late seventh century b.c.e. A number of biblical scholars have argued that the late monarchic period gave rise to the conquest story as recounted in Joshua. In this essay, I would like to pay special attention to precisely how this narrative might have functioned within the milieu of the late monarchic period, thus refining our understanding of the narrative’s contribution to the discourses of this era and our knowledge of its relationship to other narratives that were probably extant at the same time. In other words, what particular features of the narrative might have had special import in this period? Specifically, I will argue that the narrative reveals certain discursive statements about Yahweh’s cultic supremacy and about important cultic sites in late monarchic Judah, and that this is evident in particular narratival features that are present in the text

Tomographic imaging of carbon dioxide in the exhaust plume of large commercial aero-engines

We report here the first implementation of chemically specific imaging in the exhaust plume of a gas turbine typical of those used for propulsion in commercial aircraft. The method used is chemical species tomography (CST) and the target species is CO2, absorbing in the near-infrared at 1999.4 nm. A total of 126 beams propagate transverse to the plume axis, along 7 m paths in a coplanar geometry, to probe a central region of diameter ≈1.5m. The CO2 absorption spectrum is measured using tunable diode laser spectroscopy with wavelength modulation, using the second harmonic to first harmonic (2f/1f) ratio method. The engine is operated over the full range of thrust, while data are recorded in a quasi-simultaneous mode at frame rates of 1.25 and 0.3125 Hz. Various data inversion methodologies are considered and presented for image reconstruction. At all thrust levels a persistent ring structure of high CO2 concentration is observed in the central region of the measurement plane, with a raised region in the middle of the plume assumed to be due to the engine’s boat tail. With its potential to target various exhaust species, the CST method outlined here offers a new approach to turbine combustion research, turbine engine development, and aviation fuel research and development

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells

Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous gene's polyadenylation sequence and provide transcript stability. However, the spectrum of mutations generated by these vectors appears largely restricted to the last intron of target loci due to nonsense-mediated mRNA decay (NMD) making them unsuitable for gene targeting applications. Here, we present novel poly A trap vectors that overcome the effect of NMD and also employ RNA instability sequences to improve splicing efficiency. The set of random insertions generated with these vectors show a significantly reduced insertional bias and the vectors can be targeted directly to a 5′ intron. We also show that this relative positional independence is linked to the human β-actin promoter and is most likely a result of its transcriptional activity in ES cells. Taken together our data indicate that these vectors are an effective tool for insertional mutagenesis that can be used for either gene trapping or gene targeting

Observing the Evolution of the Universe

How did the universe evolve? The fine angular scale (l>1000) temperature and polarization anisotropies in the CMB are a Rosetta stone for understanding the evolution of the universe. Through detailed measurements one may address everything from the physics of the birth of the universe to the history of star formation and the process by which galaxies formed. One may in addition track the evolution of the dark energy and discover the net neutrino mass. We are at the dawn of a new era in which hundreds of square degrees of sky can be mapped with arcminute resolution and sensitivities measured in microKelvin. Acquiring these data requires the use of special purpose telescopes such as the Atacama Cosmology Telescope (ACT), located in Chile, and the South Pole Telescope (SPT). These new telescopes are outfitted with a new generation of custom mm-wave kilo-pixel arrays. Additional instruments are in the planning stages.Comment: Science White Paper submitted to the US Astro2010 Decadal Survey. Full list of 177 author available at http://cmbpol.uchicago.ed

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN