Correction: New knowledge on the antiglycoxidative mechanism of chlorogenic acid

Correction for 'New knowledge on the antiglycoxidative mechanism of chlorogenic acid' by Beatriz Fernandez-Gomez et al., Food Funct., 2015, DOI: 10.1039/c5fo00194c

PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.)

[EN] Pectin methylesterases (PMEs) are a family of enzymes involved in plant reproductive processes such as pollen development and pollen tube growth. We have isolated and characterized PsPMEP, a pea (Pisum sativum L.) pollen-specific gene that encodes a protein with homology to PMEs. Sequence analysis showed that PsPMEP belongs to group 2 PMEs, which are characterized by the presence of a processable amino-terminal PME inhibitor domain followed by the catalytic PME domain. Moreover, PsPMEP contains several motifs highly conserved among PMEs with the essential amino acid residues involved in enzyme substrate binding and catalysis. Northern blot and in situ hybridization analyses showed that PsPMEP is expressed in pollen grains from 4 days before anthesis till anther dehiscence and in pollinated carpels. In the PsPMEP promoter region, we have identified several conserved cis-regulatory elements that have been associated with gene pollen-specific expression. Expression analysis of PsPMEP promoter fused to the uidA reporter gene in Arabidopsis thaliana plants showed a similar expression pattern when compared with pea, indicating that this promoter is also functional in a non-leguminous plant. GUS expression was detected in mature pollen grains, during pollen germination, during pollen tube elongation along the transmitting tract, and when the pollen tube reaches the embryo sac in the ovule.This work was funded by grants BIO2006-09374 and BIO2009-08134 from the Spanish Ministry of Science and Innovation (MICINN). The collaboration and assistance of Julia Marin-Navarro in the catalytic activity assays of PsPMEP in yeast and Rafael Martinez-Pardo in the greenhouse is gratefully acknowledged. We would like to thank the HAPRECI consortium (COST Action FA0903) to bring us the opportunity to collaborate with other European research groups working in the field of Plant Reproduction and to select our manuscript to be published in this special issue.Gómez Jiménez, MD.; Renau Morata, B.; Roque Mesa, EM.; Polaina, J.; Beltran Porter, JP.; Cañas Clemente, LA. (2013). PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.). Plant Reproduction. 26(3):245-254. https://doi.org/10.1007/s00497-013-0220-0S245254263Bate N, Twell D (1998) Functional architecture of a late pollen promoter: pollen specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol 37:859–869Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci 316:1194–1199Bosch M, Hepler PK (2005) Pectin methylesterases and pectin dynamics in pollen tubes. Plant Cell 17:3219–3226Bosch M, Hepler PK (2006) Silencing of the tobacco pollen pectin methylesterase NtPPME1 results in retarded in vivo pollen tube growth. Planta 233:736–745Camardella L (2000) Kiwi protein inhibitor of pectin methylesterase–amino-acid sequence and structural importance of two disulfide bridges. Eur J Biochem 267:4561–4565Carpenter JL, Ploense SE, Snustad DP, Silflow CD (1992) Preferential expression of an alpha-tubulin gene of Arabidopsis in pollen. Plant Cell 4:557–571Combet C, Blanchet C, Geourjon C, Deléage G (2000) NPS@: network protein sequence analysis. Trend Biochem Sci 25:147–150De Almeida Engler J, Van Montagu M, Engler G (1998) Whole-mount in situ hybridization in plants. Methods Mol Biol (Clifton, NJ) 82:373–384De Caroli M, Lenucci MS, Di Sansebastiano GP, Dalessandro G, De Lorenzo G, Piro G (2011) Protein trafficking to the cell wall occurs through mechanisms distinguishable from default sorting in tobacco. Plant J 65(2):295–308Francis KE, Lam SY, Copenhaver GP (2006) Separation of Arabidopsis pollen tetrads is regulated by QUARTET1, a pectin methylesterase gene. Plant Physiol 142:1004–1013Futamura N, Mori H, Kouchi H, Shinohara K (2000) Male flower-specific expression of genes for polygalacturonase, pectin methylesterase and β-1,3-glucanase in a dioecious willow (Salix gilgliana Seemen). Plant Cell Physiol 41:16–26García-Sogo B, Pineda B, Castelblanque L, Antón T, Medina M, Roque E, Torresi C, Beltrán JP, Moreno V, Cañas LA (2010) Efficient transformation of Kalanchoe blossfeldiana and production of male sterile plants by engineered anther ablation. Plant Cell Rep 29:61–77García-Sogo B, Pineda B, Roque E, Antón T, Atarés A, Borja M, Beltrán JP, Moreno V, Cañas LA (2012) Production of engineered long-life and male sterile Pelargonium plants. BMC Plant Biol 12:156–162Gómez MD, Beltrán JP, Cañas LA (2004) The pea END1 promoter drives anther-specific gene expression in different plant species. Planta 219:967–981Gummadi SN, Manoj N, Kumar DS (2007) Structure and biochemical properties of pectinases. In: Polaina J, MacCabe AP (eds) Industrial enzymes: structure, function and applications. Springer, Berlin, pp 99–115. ISBN 9781402053764Gurgu L, Lafraya A, Polaina J, Marín-Navarro J (2011) Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the β-glucosidase gene (BGL1) from Saccharomycopsis fibuligera. Bioresour Technol 102:5229–5236Hamilton DA, Schwarz YH, Mascarenhas JP (1998) A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements. Plant Mol Biol 38:663–669Hewitt EJ (1966) Hoagland No. 1 solution with oligoelements. In: Sand and water culture methods used in the study of plant nutrition, 2nd edn, Technical Communication No. 22. Commonwealth Agricultural Bureaux, Farnham Royal, EnglandHothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K (2004) Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins. Plant Cell 16:3437–3447Jiang LX, Yang SL, Xie LF, Puah CS, Zhang XQ, Yang WC, Sundaresan V, Ye D (2005) VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract. Plant Cell 17:584–596Johansson K, El-Ahmad M, Friemann R, Jörnvall H, Markovic O, Eklund H (2002) Crystal structure of plant pectin methylesterase. FEBS Lett 514:243–249Jolie RP, Duvetter T, Van Loey AM, Hendrickx ME (2010) Pectin methylesterase and its proteinaceous inhibitor: a review. Carbohydr Res 345:2583–2595Kononowicz AK, Nelson DE, Singh NK, Hasegawa PM, Bressan RA (1992) Regulation of the osmotin gene promoter. Plant Cell 4:513–524Li YQ, Mareck A, Faleric C, Moscatelli A, Liu Q, Cresti M (2002) Detection and localization of pectin methylesterase isoforms in pollen tubes of Nicotiana tabacum L. Planta 214:734–740Marín-Navarro J, Gurgu L, Alamar S, Polaina J (2011) Structural and functional analysis of hybrid enzymes generated by domain shuffling between Saccharomyces cerevisiae (var. diastaticus) Sta1 glucoamylase and Saccharomycopsis fibuligera Bgl1 β-glucosidase. Appl Microbiol Biotechnol 89:121–130Markovic O, Janecek S (2004) Pectin methylesterases: sequence-structural features and phylogenetic relationships. Carbohydr Res 339:2281–2295Micheli F (2001) Pectin methylesterases: cell wall enzymes with important roles in plant physiology. Trends Plant Sci 6:414–419Mitsuda N, Takeyasu K, Sato MH (2001) Pollen-specific regulation of vacuolar H+-PPase expression by multiple cis-acting elements. Plant Mol Biol 46:185–192Okada T, Zhang Z, Russell RD, Toriyama K (1999) Localization of Bra r 1, a Ca2+-binding protein of Brassica rapa, in anthers and pollen tubes. Plant Cell Physiol 40:1243–1252Palin R, Geitmann A (2012) The role of pectin in plant morphogenesis. Bio Syst 109:397–402Pelloux J, Rustérucci C, Mellerowicz EJ (2007) New insights into pectin methylesterase structure and function. Trends Plant Sci 12:267–277Pistón F, García C, de la Viña G, Beltrán JP, Cañas LA, Barro F (2008) The pea PsEND1 promoter drives the expression of GUS in transgenic wheat at the binucleate microspores stage and during pollen tube development. Mol Breed 21:401–405Qiu X, Erickson L (1995) A pollen-specific cDNA (P65, accession no. U28148) encoding a putative pectin esterase in alfalfa (PGR95-094). Plant Physiol 109:1127Rodríguez-Llorente ID, Pérez-Hormaeche J, El Mounadi K, Dary M, Caviedes MA, Cosson V, Kondorosi A, Ratet P, Palomares AJ (2004) From pollen tubes to infection threads: recruitment of Medicago floral pectic genes for symbiosis. Plant J 39:587–598Roque E, Gómez MD, Ellull P, Wallbraun M, Madueño F, Beltrán JP, Cañas LA (2007) The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation. Plant Cell Rep 26:313–325Sambrook J, Fritsch EF, Maniatis T (1989) Hybridization of radiolabeled probes to immobilized nucleic acids. In: Ford N, Nolan C, Ferguson M (eds) Molecular cloning: a laboratory manual, 2nd edn, chap 9. Cold Spring Harbor Laboratory Press, New York, USA, pp 47–48Scognamiglio MA, Ciardiello MA, Tamburrini M, Carratore V, Rausch T, Camardella L (2003) The plant invertase inhibitor shares structural properties and disulfide bridges arrangement with the pectin methylesterase inhibitor. J Protein Chem 22:363–369Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599Tang W, Perry SE (2003) Binding site selection for the plant MADS domain protein AGL15: an in vitro and in vivo study. J Biol Chem 278:28154–28159Tian G-W, Chen M-H, Zaltsman A, Citovsky V (2006) Pollen-specific pectin methylesterase involved in pollen tube growth. Dev Biol 294:83–91Twell D, Klein TM, Fromm ME, McCormick S (1989) Transient expression of chimeric genes delivered into pollen by microprojectile bombardment. Plant Physiol 91:1270–1274Twell D, Yamaguchi J, McCormick S (1990) Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development 109:705–713Twell D, Yamaguchi J, Wing RA, Ushiba J, McCormick S (1991) Promoter analysis of genes that are co-ordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. Genes Dev 5:496–507Wakeley PR, Rogers HJ, Rozychk M, Greenland AJ, Hussey PJ (1998) A maize pectin methylesterase-gene, ZmC5, specifically expressed in pollen. Plant Mol Biol 37:187–192Wen F, Zhu Y, Hawes MC (1999) Effect of pectin methylesterase gene expression on pea root development. Plant Cell 11:1129–1140Weterings K, Schrauwen J, Wullems G, Twell D (1995) Functional dissection of the promoter of the pollen-specific gene NTP303 reveals a novel pollen-specific, and conserved cis-regulatory element. Plant J 8:55–63Wolf S, Rausch T, Greiner S (2009) The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatus. Plant J 58:361–375Woriedh M, Wolf S, Márton ML, Hinze A, Gahrtz M, Becker D, Dresselhaus T (2013) External application of gametophyte-specific ZmPMEI1 induces pollen tube burst in maize. Plant Reprod. doi: 10.1007/s00497-013-0221-zZhu C, Perry SE (2005) Control of expression and autoregulation of AGL15, a member of the MADS-box family. Plant J 41:583–59

Design and baseline characteristics of the finerenone in reducing cardiovascular mortality and morbidity in diabetic kidney disease trial

Background: Among people with diabetes, those with kidney disease have exceptionally high rates of cardiovascular (CV) morbidity and mortality and progression of their underlying kidney disease. Finerenone is a novel, nonsteroidal, selective mineralocorticoid receptor antagonist that has shown to reduce albuminuria in type 2 diabetes (T2D) patients with chronic kidney disease (CKD) while revealing only a low risk of hyperkalemia. However, the effect of finerenone on CV and renal outcomes has not yet been investigated in long-term trials. Patients and Methods: The Finerenone in Reducing CV Mortality and Morbidity in Diabetic Kidney Disease (FIGARO-DKD) trial aims to assess the efficacy and safety of finerenone compared to placebo at reducing clinically important CV and renal outcomes in T2D patients with CKD. FIGARO-DKD is a randomized, double-blind, placebo-controlled, parallel-group, event-driven trial running in 47 countries with an expected duration of approximately 6 years. FIGARO-DKD randomized 7,437 patients with an estimated glomerular filtration rate >= 25 mL/min/1.73 m(2) and albuminuria (urinary albumin-to-creatinine ratio >= 30 to <= 5,000 mg/g). The study has at least 90% power to detect a 20% reduction in the risk of the primary outcome (overall two-sided significance level alpha = 0.05), the composite of time to first occurrence of CV death, nonfatal myocardial infarction, nonfatal stroke, or hospitalization for heart failure. Conclusions: FIGARO-DKD will determine whether an optimally treated cohort of T2D patients with CKD at high risk of CV and renal events will experience cardiorenal benefits with the addition of finerenone to their treatment regimen. Trial Registration: EudraCT number: 2015-000950-39; ClinicalTrials.gov identifier: NCT02545049

Nutritional Importance of Selected Fresh Fishes, Shrimps and Mollusks to Meet Compliance with Nutritional Guidelines of n-3 LC-PUFA Intake in Spain.

Fishery products are the main source of dietary n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). Following the European Commission's request to address the risks and benefits of seafood consumption, and taking into account the great variability of nutrient and contaminant levels in fishery products, the present work aims to estimate the n-3 LC-PUFA provided per serving of selected fishes, shrimps and mollusks that are commonly consumed in Spain. This would enable the establishment of a risk-benefit analysis of fish consumption and provide recommendations for fish intake to comply with nutritional guidelines of n-3 LC-PUFA intake. We confirmed high variation in the pattern and contents of fatty acids for different species. n-6 PUFA were minor fatty acids, whereas palmitic (C16:0), oleic (C18:1 n-9), and mainly eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were the major fatty acids in the sample. Therefore, consumption of 2-3 servings per week of a variety of fishery products may contribute to compliance with the recommended daily n-3 LC-PUFA intake while maintaining an adequate balance to avoid contaminant-derived potential risks (metals and others). Taking the fatty acid content of fishery products described in this study into consideration, it is advisable to include one serving of fatty fish per week in order to meet recommended n-3 LC-PUFA levels

Effects of oils rich in eicosapentaenoic and docosahexaenoic acids on the oxidizability and thrombogenicity of low-density lipoprotein

Consumption of oily fish and fish oils is associated with protection against cardiovascular disease. Paradoxically, long-chain polyunsaturated fatty acids present in low-density lipoprotein (LDL) are suggested to be susceptible to oxidation. It is not clear whether eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have similar effects on the susceptibility of LDL to oxidation or whether they affect the thrombogenicity of oxidized LDL. This study examined the influence of highly purified preparations of EPA and DHA on LDL oxidizability and LDL-supported thrombin generation in healthy human volunteers. Forty-two healthy volunteers were randomly assigned to receive olive oil (placebo), an EPA-rich oil or a DHA-rich oil for 4 weeks at a dose of 9 g oil/day. EPA and DHA were incorporated into LDL phospholipids and cholesteryl esters during the supplementation period, but were progressively lost during ex vivo copper-mediated oxidation. Following supplementation, the EPA treatment significantly increased the formation of conjugated dienes during LDL oxidation compared with baseline, whereas the DHA treatment had no effect. Neither treatment significantly affected the lag time for oxidation, oxidation rate during the propagation phase or maximum diene production. Neither EPA nor DHA significantly affected the thrombotic tendency of oxidized LDL compared with the placebo, although DHA tended to decrease it. In conclusion, there are subtle differences in the effects of EPA and DHA on the oxidizability and thrombogenicity of LDL. DHA does not appear to increase the susceptibility of LDL to oxidation to the same degree as EPA and has a tendency to decrease LDL-supported thrombin generation. (C) 2004 Elsevier Ireland Ltd. All rights reserved

Understanding the Complexity of Water Supply System Governance: A Proposal for a Methodological Framework

The question of how the complexity of water governance may be understood beyond a heuristic concept remains unanswered. In this paper, we propose a Water Governance Complexity Framework to address the complexity of water governance. Through a literature review, rapid surveys, and 79 semi-structured interviews, we propose how this framework may be operationalized using different proxies and by applying it to the case of the water supply system for domestic use in Oaxaca, Mexico. In places such as the rural communities of Oaxaca, where the state plays a partially absent role in the water supply, we found legal pluralism and diverse formal and informal stakeholders in a multi-level structure. At the local level, four modes of governance were identified, resulting from seven institutional change trajectories. These trajectories result from linear (alignment) and non-linear (resistance and adaptation) interactions between local, state, and national institutions over different periods. We provide a pragmatic framework to understand complexity through the organization and historical configurations of water governance that may be applied globally, providing a necessary starting point and solid foundation for the creation of new water policies and law reforms or transitions to the polycentric governance model to ensure the human right to water and sanitation

From green technology to functional olive oils: assessing the best combination of olive tree-related extracts with complementary bioactivities

Our aim was to assess the combination of olive tree-related extracts with the most favorable profile of in vitro bioactive properties. We tested the antioxidant (increment of low-density lipoprotein resistance against oxidation), vasoactive (promotion of nitric oxide release and decrease of endothelin-1 production in human umbilical vein endothelial cells), anti-inflammatory (decrease of the endothelial production of vascular cell adhesion molecule-1), and antithrombotic (reduction of the endothelial release of plasminogen activator inhibitor-1) capacities of six phenolic extracts and three triterpenic acid solutions (Ps and Ts, respectively). We tested extracts alone and in combination, at nutritional (Ps: 0.05-0.5 μmol/L; Ts: 0.001-0.1 μmol/L) and nutraceutical doses (Ps: 1-10 μmol/L; Ts: 0.25-10 μmol/L). The combination of Ps rich in 3,4-dihydroxyphenylglycol (76%, P2), hydroxytyrosol (95%, P3), and oleuropein (70%, P4) (final nutritional concentration: 0.15 μmol/L; final nutraceutical concentration: 3 μmol/L) was the best in order to prepare functional products and nutraceuticals with cardioprotective properties, despite the fact that the isolated extract with the greatest in vitro properties was P5 (75% oleocanthal), suggesting a potential synergistic effect among different olive components

Does increased intake of salmon increase markers of of oxidative stress in pregnant women?

The Salmon in Pregnancy Study provided two meals of salmon per week to pregnant women from week 20 of gestation; the control group maintained their habitual diet low in oily fish. Salmon is a rich source of marine n-3 fatty acids. Since marine n-3 fatty acids may increase oxidative stress, we investigated whether increased salmon consumption could affect markers of oxidative stress in mid and late pregnancy. Urinary 8-iso-prostaglandin F2?, urinary 8-hydroxy-2?-deoxyguanosine, and plasma lipid peroxide concentrations did not change from week 20 to 38 of pregnancy and were not altered by increased consumption of salmon. Thus, increased intake of salmon during pregnancy does not increase oxidative stress, as judged by the markers of oxidative damage to lipids and DNA measured herein

Docosahexaenoic and Eicosapentaenoic Intervention Modifies Plasma and Erythrocyte Omega-3 Fatty Acid Profiles But Not the Clinical Course of Children With Autism Spectrum Disorder: A Randomized Control Trial.

The pathogenesis of autism spectrum disorder (ASD) is under investigation and one of the main alterations relates to the metabolic and inflammatory system dysfunctions. Indeed, based on a possible deficit of omega-3 fatty acids (FAs) of patients with ASD and looking for an anti-inflammatory effect, dietary supplements with omega-3 fatty acids have been proposed. We aimed to evaluate differences in plasma and erythrocyte FA profiles and plasma cytokines in patients with infantile ASD after supplementation with docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids or placebo and both compared at baseline with a reference healthy group. A double-blind, randomized placebo-controlled intervention with DHA/EPA for 6 months was carried out in 54 children between 2 and 6 years diagnosed with ASD. They were selected and randomly assigned into two groups: 19 children received 800 mg/day of DHA and 25 mg/day of EPA, or placebo. In addition, another reference group of 59 healthy children of the same age was included. Plasma lipids and cytokines, and FA profiles in plasma and erythrocytes were measured at baseline and after 6 months of treatment in ASD children, and at baseline in the reference group. There were no differences in demographic, anthropometric characteristics, and omega-3 intake between the healthy reference group and the ASD children at baseline. Children with ASD showed the higher plasma percentages of palmitic acid and total saturated FA and lower total omega-6 polyunsaturated FA (PUFA) compared with healthy children. An increased level of DHA and reduced EPA level in erythrocytes were detected in the ASD group vs. the reference group. After 6 months of treatment, the ASD group that received DHA enriched product significantly increased the plasma and erythrocyte percentages of DHA, but no differences were observed in the clinical test scores and other parameters as plasma cytokines between the two groups of ASD related to the intervention. Spanish children with ASD exhibit an appropriate omega-3 FA status in plasma and erythrocytes. Neither a clinical improvement of ASD children nor a better anti-inflammatory or fatty acid state has been found after an intervention with DHA/EPA for 6 months. So, the prescription of n-3 LC-PUFA and other dietary supplements in ASD should be only indicated after a confirmed alteration of FA metabolism or omega-3 LC-PUFA deficiency evaluated by specific erythrocyte FA. [www.ClinicalTrials.gov], identifier [NCT03620097]