Evaluación de la capacidad bioindicadora de plantas y artópodos en la toxicidad del metil-mercurio

Tesis de la Universidad Complutense de Madrid, Facultad de Veterinaria, Departamento de Toxicología y Farmacología, leída el 09-01-2002Sección Deptal. de Farmacología y Toxicología (Veterinaria)Fac. de VeterinariaTRUEpu

Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins

Spanish Ministry of Economy and Competitiveness (Grant BFU2015-70052-R). Spanish Ministry of Science, Innovation, and Universities, the Spanish State Research Agency, the European Regional Development Fund (RTI2018-099985-B-I00, MCIU/AEI/FEDER, UE), the CIBER of Respiratory Diseases (CIBERES), an initiative from the Spanish Institute of Health Carlos III (ISCIII). PTDC/BIA-MIB/31730/2017. IF/00023/2012. Applied Molecular Biosciences Unit (UCIBIO) (Financed by FCT-MCTES, UID/Multi/04378/2013/2019).Bacterial surfaces are decorated with distinct carbohydrate structures that may substantially differ among species and strains. These structures can be recognized by a variety of glycan-binding proteins, playing an important role in the bacteria cross-talk with the host and invading bacteriophages, and also in the formation of bacterial microcolonies and biofilms. In recent years, different microarray approaches for exploring bacterial surface glycans and their recognition by proteins have been developed. A main advantage of the microarray format is the inherent miniaturization of the method, which allows sensitive and high-throughput analyses with very small amounts of sample. Antibody and lectin microarrays have been used for examining bacterial glycosignatures, enabling bacteria identification and differentiation among strains. In addition, microarrays incorporating bacterial carbohydrate structures have served to evaluate their recognition by diverse host/phage/bacterial glycan-binding proteins, such as lectins, effectors of the immune system, or bacterial and phagic cell wall lysins, and to identify antigenic determinants for vaccine development. The list of samples printed in the arrays includes polysaccharides, lipopoly/lipooligosaccharides, (lipo)teichoic acids, and peptidoglycans, as well as sequence-defined oligosaccharide fragments. Moreover, microarrays of cell wall fragments and entire bacterial cells have been developed, which also allow to study bacterial glycosylation patterns. In this review, examples of the different microarray platforms and applications are presented with a view to give the current state-of-the-art and future prospects in this field.publishersversionpublishe

Análisis de los activos intangibles desde una perspectiva contable y fiscal

Los estados contables no recogen adecuadamente la valoración de los activos intangibles, por lo que se produce una divergencia entre los estados financieros y el valor de mercado de las empresas. En el presente artículo, se parte de la revisión del concepto de activo intangible para analizar posteriormente el tratamiento contable y fiscal dado a los mismos en el caso español, haciendo asimismo referencia al entorno internacional

Application of a random regression model for genetic parameters estimation of race performance in young Spanish Trotter horses: Preliminary analysis

Un total de 36212 registros deportivos recopilados entre 1990-2006 en 2325 caballos trotadores en España fueron estudiados mediante Modelos de Regresión Aleatoria (RRM) para la estimación de los componentes de los parámetros genéticos del rendimiento funcional en caballos jóvenes (2-4 años) de esta raza en carreras de distancias entre los 1600 y 2750 metros. El pedigrí de cada animal ha sido completado hasta la cuarta generación, generando una figura de 9201 individuos. La variable dependiente analizada ha sido el tiempo medio en recorrer un kilómetro en las diferentes distancias de carrera en las que han participado. El hipódromo-fecha de carrera (405) y el sexo (3: macho, hembra y castrado) se han incluido como efectos fijos en el modelo, también un polinomio de Legendre de orden 2 fue incorporado como efecto fijo. Los animales (9201), el conductor (1007) y los efectos ambientales permanentes causados por los registros de las participaciones repetidas de un mismo animal han sido incorporados como variables aleatorias, aplicando en ambos casos un polinomio Legendre de orden 1. Los resultados muestran que los componentes de la varianza aditiva y el nivel de heredabilidad (h2) disminuyen a medida que aumenta la distancia de la carrera (oscilando entre una h2 de 0,32 a los 1600 m y de 0,12 a los 2700 m). Las correlaciones genéticas entre las distancias más próximas han sido superiores. La metodología de estimación utilizada permite una función de los valores genéticos con la que se pueden estimar los valores de cría (BV) de cada animal en todo el recorrido de distancias analizadas. Se ha estimado una variabilidad muy elevada para los BV y se han detectado importantes diferencias en la forma de respuesta de los caballos en la trayectoria de la curva de distancias. El uso de un RRM en los programas de cría para la valoración genética del rendimiento en caballos Trotadores Españoles está muy recomendado.A total of 36212 racing performance data recorded between 1990 and 2006 from 2325 Trotter horses in Spain were studied by Random Regression Model (RRM), in order to estimate the genetic parameters for race performance in young horses (2-4 years old) of this breed by distances ranging between 1600-2750m. The pedigree of each horse was extended until the fourth generation, with a total of 9201 individuals. Racing time per kilometre over the trajectory of distances was the dependent variable. The hippodrome-date of race (405), sex (3: male, female and gelding) and a second order Legendre polynomial were the fixed effects. The animals (9201), the driver (1007) and the permanent environmental effects due to repetitions of records from the same animals were incorporated as random with a Legendre polynomial of order 1 in the model. According to our results, the additive variance components and the heritability (h2) decrease as race distance increase, (ranging between h2=0.32 at 1600 m and h2=0.12 at 2700 m). The genetic correlations between close distances were higher. With these results, it was possible to estimate the Breeding Value (BV) for all animals and all distances represented in the data set. A very high variability was estimated for the BV and important differences were detected in the form of responses of the horses along the trajectory of distances. The use of RRM is highly recommended in the breeding programs of racing performance of the Trotter horse in Spai

Comparison of coal chars prepared in different devices at similar temperature

The characteristics of chars prepared in both a drop tube furnace (DTF) and a flat flame burner (FFB) at 1300 ºC from seven coals of different rank and maceral composition are the subject of this study. Coal samples sized and sieved to 36-75 μm were fed. The flame temperature of the FFB was achieved with a mixture of methane, air and oxygen whereas two different gas compositions were used in the DTF to account for sub-stoichiometric (2.5% O2) and close to stoichiometric (10% O2) oxygen levels. Determination of micropore surface area was carried out by CO2 adsorption isotherms at 0°C and char reactivity to air was measured at 550 °C in a thermogravimetric analyser (TGA). Overall similar combustion trends were obtained in both devices with burnout decreasing as coal rank increases and CO2 surface areas decreasing up to the medium volatile bituminous coal rank and increasing again for the anthracites. The results reveal a reasonable agreement between burnouts of FFB chars and low oxygen DTF chars whereas higher burnouts were obtained for higher oxygen DTF chars. The CO2 surface areas of the chars reasonably scattered without any systematic trend. The intrinsic reactivities of both series of chars from the DTF were similar to the corresponding FFB chars. Increasing coal rank, intrinsic reactivity of chars from the different combustion conditions decreased.The Principality of Asturias (Principado de Asturias), Project PC04-03 and the Ministry for Education (Ministerio de Educación), Project PSE2-2005Peer reviewe

Eu3+ luminescence in high charge mica: an in situ probe for the encapsulation of radioactive waste in geological repositories

Isolation of high-level radioactive waste (HLW) in deep geological repositories (DGR) through a multibarrier concept is the most accepted approach to ensure long-term safety. Clay minerals are one of the most promising materials to be used as engineered barriers. In particular, high charge micas, as components of the engineered barrier, show superselectivity for some radioactive isotopes and a large adsorption capacity, which is almost twice that of the other low charge aluminosilicates. In addition, high charge micas are optimum candidates for decontamination of nuclear waste through two different mechanisms; namely an ion exchange reaction and a nonreversible mechanism involving the formation of new stable crystalline phases under hydrothermal conditions. In this work, we report a new in situ optical sensor based on the incorporation of Eu3+ in these high charge micas for tracking the long-term physical-chemical behavior of HLW contaminants in DRG under mild hydrothermal conditions. The incorporation of Eu3+ into the interlayer space of the mica originates a well resolved green and red luminescence, from both the 5D1 and 5D0 excited states, respectively. The formation of new crystalline phases under hydrothermal conditions involves important changes in the Eu3+ emission spectra and lifetime. The most interesting features of Eu3+ luminescence to be used as an optical sensor are (1) the presence or absence of the Eu3+ green emission from the 5D1 excited state, (2) the energy shift of the 5D0 → 7F0 transition, (3) the crystal-field splitting of the 7F1 Eu3+ level, and (4) the observed luminescence lifetimes, which are directly related to the interaction mechanisms between the lanthanide ions and the silicate network.Funding from projects MAT2015-63929-R, MAT2015-69508-P, PI16/00496, and NVAL16/17-IDIVAL is gratefully acknowledged

Exploring the link between free and open source software and the collaborative economy: A Delphi-based scenario for the year 2025

Despite the growth experienced by the Collaborative Economy in recent years, there are still unexplored gaps within this phenomenon. One of the areas of study with scarce literature is linked with the impact of the Information and Communication Technologies based on collaborative environments, such as Free and Open Source Software, on the spread of the Collaborative Economy. Some questions are raised, such as: (1) To what extent do organizations linked with Collaborative Economy make use of Free and Open Source Software?, (2) What are the incentives that motivate the implementation of Free and Open Source Software in Collaborative Economy companies?, (3) What use do Collaborative Economy companies give to Free and Open Source Software?, and (4) Is there a greater use of Free and Open Source Software expected for the coming years among these organizations? To answer these questions, a study based on the Delphi method has been designed. To this end, a panel of 15 high-level experts in the field was formed. From the consensus of the experts, a significant role for Free and Open Source Software in the different collaborative components and industries is evident, with the current levels practically being maintained by the year 2025.Universidad Pablo de Olavid

Papel de NFkB como posible biomarcador en el desarrollo de cáncer de pulmón en pacientes previamente diagnosticados de EPOC

Comunicaciones a congreso