34 research outputs found

    Requirements Management and its Application to the Corporate Website of the University

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    Requirements management understood as recording and analysis of the claims made by the stakeholders as well as the interaction with them is essential to maintain the quality of the project. The effective requirements management involves their collection, classification, and prioritization, analysis, setting the objectives to meet such requirements, and storing the information on them. The paper reviews the research on the requirements management. The process can apply to different fields. For its effective functioning it is necessary to develop the requirements management system which should verify and analyze the requirements entering it. The work examines the construction features of this system and focuses on the specific management of the requirements for the corporate websites of universities

    Optimization of Enzymatic Logic Gates and Networks for Noise Reduction and Stability

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    Biochemical computing attempts to process information with biomolecules and biological objects. In this work we review our results on analysis and optimization of single biochemical logic gates based on enzymatic reactions, and a network of three gates, for reduction of the "analog" noise buildup. For a single gate, optimization is achieved by analyzing the enzymatic reactions within a framework of kinetic equations. We demonstrate that using co-substrates with much smaller affinities than the primary substrate, a negligible increase in the noise output from the logic gate is obtained as compared to the input noise. A network of enzymatic gates is analyzed by varying selective inputs and fitting standardized few-parameters response functions assumed for each gate. This allows probing of the individual gate quality but primarily yields information on the relative contribution of the gates to noise amplification. The derived information is then used to modify experimental single gate and network systems to operate them in a regime of reduced analog noise amplification.Comment: 7 pages in PD

    Enzymatic Logic Gates with Noise-Reducing Sigmoid Response

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    Biochemical computing is an emerging field of unconventional computing that attempts to process information with biomolecules and biological objects using digital logic. In this work we survey filtering in general, in biochemical computing, and summarize the experimental realization of an AND logic gate with sigmoid response in one of the inputs. The logic gate is realized with electrode-immobilized glucose-6-phosphate dehydrogenase enzyme that catalyzes a reaction corresponding to the Boolean AND functions. A kinetic model is also developed and used to evaluate the extent to which the performance of the experimentally realized logic gate is close to optimal.Comment: 14 pages, 2 figures, PD

    Spin configurations in circular and rectangular vertical quantum dots in a magnetic field: Three-dimensional self-consistent simulation

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    The magnetic field dependence of the electronic properties of \textit{real} single vertical quantum dots in circular and rectangular mesas is investigated within a full three-dimensional multiscale self-consistent approach without any {\it \'a priori} assumptions about the shape and strength of the confinement potential. The calculated zero field electron addition energies are in good agreement with available experimental data for both mesa geometries. Charging diagrams in a magnetic field for number of electrons up to five are also computed. Consistent with the experimental data, we found that the charging curves for the rectangular mesa dot in a magnetic field are flatter and exhibit less features than for a circular mesa dot. Evolution of the singlet-triplet energy separation in the two electron system for both dot geometries in magnetic field was also investigated. In the limit of large field, beyond the singlet-triplet transition, the singlet-triplet energy difference continues to become more negative in a circular mesa dot without any saturation within the range of considered magnetic fields whilst it is predicted to asymptotically approach zero for the rectangular mesa dot. This different behavior is attributed to the symmetry "breaking" that occurs in the singlet wave-functions in the rectangular mesa dot but not in the circular one.Comment: 12 pages, 8 gifure

    Enzymatic AND Logic Gates Operated Under Conditions Characteristic of Biomedical Applications

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    Experimental and theoretical analyses of the lactate dehydrogenase and glutathione reductase based enzymatic AND logic gates in which the enzymes and their substrates serve as logic inputs are performed. These two systems are examples of the novel, previously unexplored, class of biochemical logic gates that illustrate potential biomedical applications of biochemical logic. They are characterized by input concentrations at logic 0 and 1 states corresponding to normal and abnormal physiological conditions. Our analysis shows that the logic gates under investigation have similar noise characteristics. Both significantly amplify random noise present in inputs, however we establish that for realistic widths of the input noise distributions, it is still possible to differentiate between the logic 0 and 1 states of the output. This indicates that reliable detection of abnormal biomedical conditions is indeed possible with such enzyme-logic systems.Comment: PDF, 29 page

    Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with Low Noise

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    Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal

    Towards Biochemical Filter with Sigmoidal Response to pH Changes: Buffered Biocatalytic Signal Transduction

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    We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid-the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element" for increasing the complexity of biochemical information processing systems. Evaluation of an optimal regime for quality filtering is accomplished in the framework of a kinetic rate-equation model.Comment: PDF, 23 page

    Green roofs in Saint Petersburg

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    The aim of this work was to investigate Russian and foreign projects of green roofs, evaluate the possibility of building a green roof on the building Gidrokorpus and write recommendations for its installation and maintenance. Construction of green roofs is becoming now one of the fastest growing areas of landscape architecture and is becoming increasingly popular in St. Petersburg. Considering the insufficient number of domestic works of green roofs in local climatic conditions, a number of environmental advantages of extensive green roofs with different types of vegetation in conditions of St. Petersburg was investigated. Also the possibility of building a green roof on the Gidrokorpus was evaluated and recommendations for its installation were developed. Ekaterina Zalata was responsible for Chapter 2 (Green roofs now and in the past) and Chapter 4 (Green roof buildings in Saint Petersburg). Dmitriy Melnikov was responsible for Chapter 3 (Green roofs in construction). Chapter 5 (Construction solutions for the roof system of Gidrocorpus) was written by both Ekaterina and Dmitriy. As the result a construction decision for the roof of Gidrocorpus was provided to the company Delta-t

    К РУССКОЯЗЫЧНОЙ НАД-ЛИТЕРАТУРЕ: СОЗДАВАЯ СУБЪЕКТИВНОСТИ, ПРОСТРАНСТВА И ТЕМПОРАЛЬНОСТИ В ПОСТСОВЕТСКОЙ КАЗАХСТАНСКОЙ РУССКОЯЗЫЧНОЙ ЛИТЕРАТУРЕ

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    This thesis is devoted to the analysis of literary works by a number of the leading post-Soviet Russophone Kazakhstani writers: Anuar Duisenbinov, Bakhyt Kairbekov, Diusenbek Nakipov, Nikolai Verёvochkin, Il’ia Odegov and Iurii Serebrianskii. Kazakhstan is a country where Russian literature has been developing quite successfully since the collapse of the USSR. There has been a transformation of writing in Russian in Kazakhstan since the country’s independence – with the rise of the new generation of the writers in the 2000s, Russian literature in Kazakhstan transformed into Russophone Kazakhstani literature. In this thesis, I argue for the difference between the younger and older generations of the contemporary Russophone Kazakhstani writers – the latter is focused on post-traumatic sense of loss and absence, while the former is characterized by a more positive identification concentrated on the new national post-independent realities of Kazakhstan..

    Physical and Electrical Properties of Yttria-Stabilized Zirconia Thin Films Prepared by Radio Frequency Magnetron Sputtering

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    This paper presents the electrophysical characteristics of a 7 mol.% yttria-stabilized zirconia (YSZ) thin film deposited by radio-frequency magnetron sputtering. In order to form the crystallinestructure, the deposited films were annealed in air over a temperature range of 700 ÷ 900 °C. By XRD analysis it was established that as the deposited films were amorphous, they crystallized into a pure cubic structure as a result of annealing in air at a temperature above 820 °C.The electrophysical properties of YSZ films were investigated on structures such¨as Ni/YSZ/Pt/Ti/Si and Ni/YSZ/Si. Film features ? > 20 and tg ? < 0.05 were obtained. An estimate of the capacity-voltage characteristic proved that the Ni/YSZ/Si structures possessed a hysteresis. This hysteresis resulted from the drift of the mobile ions in the YSZ film. High-temperature ionic conductivity of the stabilized zirconia was determined by the measurements of the electric resistivity of the YSZ films at 1 kHz over the temperature range from ambient to 800 °C. The YSZ film conductivity obtained was 1.96 × 10-2 S/cm under 800 °C
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