A formação em agronomia na educação do campo e os impactos para o desenvolvimento das áreas de reforma agrária

O presente artigo busca investigar qual o impacto da formação em Agronomia pelo Programa Nacional de Educação na Reforma Agrária (Pronera) no desenvolvimento das áreas de reforma agrária. Foi escolhido o curso de Agronomia da Universidade Federal do Triângulo Mineiro (UFTM), campus Iturama, para este estudo. O objetivo geral do trabalho é analisar tal impacto. Os objetivos específicos são avaliar o desenho da política pública do Pronera e elenco de impactos, assim como comparar os projetos pedagógicos das turmas regulares da universidade e do Pronera. Para tanto, foi realizada pesquisa bibliográfica e análise documental referente ao curso e às atividades dos educandos. Após a análise, percebeu-se que os educandos são o elemento principal de impacto nas áreas de reforma agrária ao se considerar o seu caráter transformador, sua defesa da agricultura familiar e seu diálogo de saberes.35 PáginasGestão PúblicaMeio Ambient

Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5

Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response—usually investigated by antibody assays in serum—may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals

PROJETO DE ENSINO DE LÍNGUA ESPANHOLA: INFOGRÁFICO

Neste trabalho, discutimos o papel do letramento científico como forma de construção da cidadania na escola. Fazemos um relato do trabalho desenvolvido no âmbito do Subprojeto do Programa Institucional de Bolsa de Iniciação à Docência - PIBID Letras-Espanhol, na Universidade do Vale do Rio dos Sinos – UNISINOS, buscando socializar os resultados alcançados no desenvolvimento de um projeto de ensino de Língua Espanhola que explora o gênero infográfico. O projeto foi implementado em uma escola de ensino médio em São Leopoldo, Rio Grande do Sul. Para abordar o letramento científico, baseamo-nos em autores como Santos (2007), Demo (2010) e Souza (2009, 2011), que tratam de conhecimentos relacionados à educação científica, à divulgação científica e ao gênero infográfico. O resultado do projeto evidenciou que os alunos foram capazes de captar informações em língua espanhola, transformando-as em infográficos, respeitando as características do gênero e produzindo um novo conhecimento

“Anjos do COVID-19”, monitoramento de militares e dependentes: relato de experiência: “Angels of COVID-19”, monitoring of military and dependents: experience report

O objetivo deste trabalho foi relatar a vivência de um projeto de apoio assistencial por telemonitoramento de militares e seus dependentes. Método: Relato de experiência da implementação de um sistema de acompanhamento, através de ligações telefônicas e de mensagens por aplicativo, aos usuários do Esquadrão de Saúde de Curitiba da Força Aérea Brasileira, como estratégia de atenção humanizada aos pacientes durante a Pandemia de Covid-19, no período de março de 2020 a outubro de 2021. Resultados: Foram registrados 2.376 teleatendimentos, abrangendo militares e seus dependentes. O contato através do aplicativo  foi uma ótima solução para proporcionar a escuta desses pacientes durante o período de isolamento. Conclusão: A atenção qualificada dada durante os dias de isolamento foi relevante não só devido ao esclarecimento de dúvidas sobre os sintomas e a evolução da doença, como também aos cuidados emocionais voltados às pessoas em quarentena, destacando-se como um mecanismo de conforto frente à tensão da doença em si. O telemonitoramento foi fundamental para evitar o congestionamento do pronto-atendimento médico e no conforto das pessoas, as quais viviam apreensivas com a situação e o risco diário causado pelo coronavírus

Does aortic valve repair in valve-sparing aortic root reconstruction compromise the longevity of the procedure?

OBJECTIVES: The effect of performing aortic valve repair in combination with valve-sparing operation on the length of time for which patients are free from reoperation is unclear. The objective of this study was to determine if the performance of aortic valve repair during valve-sparing operation modified the freedom from reoperation time. METHODS: From January 2003 to July 2014, 78 patients with a mean age of 49±15 years underwent valve-sparing operation. Sixty-eight percent of these patients were male. Twenty-two (28%) aortic valve repair procedures were performed in this patient population. In the aortic valve repair + valve-sparing operation group, 77.3% of patients had moderate/severe aortic insufficiency, while in the valve-sparing operation group, 58.6% of patients had moderate/severe aortic insufficiency (ns = not significant). Additionally, 13.6% of patients in the aortic valve repair + valve-sparing operation group had functional class III/IV, while 14.2% of patients in the valve-sparing operation group had functional class III/IV (ns). RESULTS: The in-hospital and late mortality rates, for the aortic valve repair + valve-sparing operation and valve-sparing operation groups were similar, as they were 4.5% and 3.6%; and 0% and 1.8%, respectively. In the aortic valve repair + valve-sparing operation group, 0% of patients presented moderate/severe aortic insufficiency during late follow-up, while in the valve-sparing operation group, 14.2% of patients presented with moderate/severe aortic insufficiency during this period (ns). In the aortic valve repair + valve-sparing operation group, 5.3% of patients presented with functional class III/IV, while in the valve-sparing operation group, 4.2% of patients presented with functional class III/IV (ns). In the aortic valve repair + valve-sparing operation group, 0% of patients required reoperation, while in the valve-sparing operation group, 3.6% of patients required reoperation over a mean follow-up period of 1621±1156 days (75 patients). CONCLUSION: Valve-sparing operation is a safe and long-lasting procedure and performance of aortic valve repair when necessary does not increase risk of reoperation on the aortic valve

Circulation of Dengue virus serotype 1 genotype V and Dengue virus serotype 2 genotype III in Tocantins state, Northern Brazil, 2021–2022

In Brazil, the state of Tocantins, located in north-central Brazil, has experienced a significant number of cases of arboviral disease, particularly Dengue virus (DENV). This study aimed to deepen the knowledge on DENV circulation within that state by conducting full genome sequencing of viral genomes recovered from 61 patients between June 2021 and July 2022. There were a total of 8807 and 20,692 cases in 2021 and 2022, respectively, as reported by the state’s Secretary of Health. Nucleotide sequencing confirmed the circulation of DENV serotype 1, genotype V and DENV serotype 2, genotype III in the State. Younger age groups (4 to 43 years old) were mostly affected; however, no significant differences were detected regarding the gender distribution of cases in humans. Phylogenetic analysis revealed that the circulating viruses belong to DENV-1 genotype V American and DENV-2 genotype III Southeast Asian/American. The Bayesian analysis of DENV-1 genotype V genomes sequenced here are closely related to genomes previously sequenced in the state of São Paulo. Regarding the DENV-2 genotype III genomes, these clustered in a distinct, well-supported subclade, along with previously reported isolates from the states of Goiás and São Paulo. The findings reported here suggest that multiple introductions of these genotypes occurred in the Tocantins state. This observation highlights the importance of major population centers in Brazil on virus dispersion, such as those observed in other Latin American and North American countries. In the SNP analysis, DENV-1 displayed 122 distinct missense mutations, while DENV-2 had 44, with significant mutations predominantly occurring in the envelope and NS5 proteins. The analyses performed here highlight the concomitant circulation of distinct DENV-1 and -2 genotypes in some Brazilian states, underscoring the dynamic evolution of DENV and the relevance of surveillance efforts in supporting public health policies

Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection

Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4+CD25+Foxp3+ T regulatory (TREG) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral TREG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3−/−) TREG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both TREG cells and T effector (TEFF) cells from Lgals3−/− mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both TREG and TEFF cells from uninfected Lgals3−/− mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4+CD25+Foxp3+ TREG cells and alters the course of L. major infection.Fil: Fermino, Marise L.. Universidade de Sao Paulo; BrasilFil: Dias, Fabrício C.. Universidade de Sao Paulo; BrasilFil: Lopes, Carla D.. Universidade de Sao Paulo; BrasilFil: Souza, Maria A.. Universidade de Sao Paulo; BrasilFil: Cruz, Ângela K.. Universidade de Sao Paulo; BrasilFil: Liu, Fu Tong. University of California at Davis; Estados UnidosFil: Chammas, Roger. Universidade de Sao Paulo; BrasilFil: Roque Barreira, Maria C.. Universidade de Sao Paulo; BrasilFil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Bernardes, Emerson S.. Universidade de Sao Paulo; Brasi

Clinical-pathological and sociodemographic factors associated with the distant metastasis and overall survival of oral cavity and oropharynx squamous cell carcinoma

The objective of this study was to evaluate the influence of clinical-pathological and sociodemographic factors on the prevalence of distant metastasis (DM) and overall survival in patients with oral cavity and oropharynx squamous cell carcinoma (OOSCC). Cross-sectional study based on the records of 404 OOSCC patients evaluated for DM, covering the period 2000-2014. We analysed the influence of age, sex, level of schooling, primary tumor subsite, treatment, marital status, family history of cancer, history of smoking and alcohol consumption, type of health care coverage (private vs. public) and overall survival. Findings were submitted to Fisher?s exact test, Pearson?s chi-squared test, Mantel-Cox log-rank testing and multinomial and Cox regression analysis (SPSS v. 20.0; p65 years (p=0.046), indigenous/brown racial type (p=0.045), palliative treatment (p=0.035) and DM (p=0.048). Lymph node metastasis independently increased the prevalence of DM and, along with male sex, older age, brown racial type and palliative treatment, was independently associated with poor prognosis in patients with OOSCC

Genomic epidemiology reveals the circulation of the Chikungunya virus east/central/south african lineage in Tocantins State, north Brazil

The chikungunya virus (CHIKV) is a mosquito-borne virus of the family Togaviridae transmitted to humans by Aedes spp. mosquitoes. In Brazil, imported cases have been reported since June 2014 through two independent introductions, one caused by Asian Lineage in Oiapoque, Amapá state, North Region, and another caused by East/Central/South African (ECSA) in Feira de Santana, Bahia state, Northeast Region. Moreover, there is still limited information about the genomic epidemiology of the CHIKV from surveillance studies. The Tocantins state, located in Northern Brazil, reported an increase in the number of CHIKV cases at the end of 2021 and the beginning of 2022. Thus, to better understand the dispersion dynamics of this viral pathogen in the state, we generated 27 near-complete CHIKV genome sequences from four cities, obtained from clinical samples. Our results showed that the newly CHIKV genomes from Tocantins belonged to the ECSA lineage. Phylogenetic reconstruction revealed that Tocantins’ strains formed a single well-supported clade, which appear to be closely related to isolates from the Rio Grande do Norte state (Northeast Brazil) and the Rio de Janeiro state (Southeast Brazil), that experienced an explosive ECSA epidemic between 2016–2019. Mutation analyses showed eleven frequent non-synonymous mutations in the structural and non-structural proteins, indicating the autochthonous transmission of the CHIKV in the state. None of the genomes recovered within the Tocantins samples carry the A226V mutation in the E1 protein associated with increased transmission in A. albopictus. The study presented here highlights the importance of continued genomic surveillance to provide information not only on recording mutations along the viral genome but as a molecular surveillance tool to trace virus spread within the country, to predict events of likely occurrence of new infections, and, as such, contribute to an improved public health service