Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers.

ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, is frequently mutated in cancer. Deficiency in its homolog ARID1B is synthetically lethal with ARID1A mutation. However, the functional relationship between these homologs has not been explored. Here, we use ATAC-seq, genome-wide histone modification mapping, and expression analysis to examine colorectal cancer cells lacking one or both ARID proteins. We find that ARID1A has a dominant role in maintaining chromatin accessibility at enhancers, while the contribution of ARID1B is evident only in the context of ARID1A mutation. Changes in accessibility are predictive of changes in expression and correlate with loss of H3K4me and H3K27ac marks, nucleosome spacing, and transcription factor binding, particularly at growth pathway genes including MET. We find that ARID1B knockdown in ARID1A mutant ovarian cancer cells causes similar loss of enhancer architecture, suggesting that this is a conserved function underlying the synthetic lethality between ARID1A and ARID1B

Development of microsatellites markers for the deep coral Madracis myriaster (Pocilloporidae: Anthozoa)

In 2013 Colombia made an important step towards the construction and management of Marine Protected Areas (MPAs) by establishing the first Deep Corals National Park (PNNCP). Inside this MPA, the coral Madracis myriaster (Cnidaria: Pocilloporidae) was found as the main reef builder, offering habitat for many species of fish and invertebrates. In order to improve the study of deep-sea coral habitats, their connectivity and prospective management, nine new genetic markers (microsatellites) were developed for M. myriaster and tested in samples from PNNCP. We present the assessment of these markers, with a specificity for the deep coral, and its prospective use in future analysis for the PNNCP and other areas in the Caribbean and the Atlantic, where M. myriaster is reported. We also include an additional taxonomic analysis performed on samples of M. myriaster using scanning electron microscopy

Osteomalacia tumoral

La osteomalacia tumoral (OT) es un síndrome paraneoplásico en el cual el paciente presenta dolor óseo, fracturas y debilidad muscular. Es producido principalmente por una elevación de los niveles en sangre del factor de crecimiento fibroblástico 23 (FGF-23). En la OT, el FGF-23 es secretado por tumores mesenquimales, los cuales se caracterizan por ser de tamaño pequeño y de lento crecimiento. El FGF-23 actúa a nivel de los túbulos renales afectando la reabsorción de fósforo, lo cual conduce a hipofosfatemia y a bajos niveles de 1,25-dihidroxivitamina D [1,25(OH)2 D]. El tratamiento de la OT es la resección del tumor. Debido a las características de estos tumores muchas veces no pueden ser localizados o son quirúrgicamente irresecables y requieren tratamiento médico a largo plazo, consistente en sales de fósforo y calcitriol. La adherencia al tratamiento muchas veces resulta difícil debido a las reacciones secundarias, así como también a las complicaciones asociadas.Tumor-induced osteomalacia (TIO) is a paraneoplastic syndrome in which patients present with bone pain, fractures and muscle weakness. The cause is excess production of fibroblast growth factor 23 (FGF-23). In TIO, FGF- 23 is secreted by mesenchymal tumors, which are small and grow slowly. FGF-23 acts at the renal tubule and impairs phosphate reabsorption, leading to hypophosphatemia and low levels of 1,25-dihidroxivitamin D [1,25(OH)2 D]. The treatment of TIO is surgical resection of the causative tumor. For tumors that cannot be located or surgically resected, medical treatment with oral phosphate supplements and calcitriol is usually successful. However, adherence in some cases is difficult due to secondary reactions or treatment complications.Fil: Mastaglia, Silvina Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Somoza, Julia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: González, Diana C.. Centro de Osteopatías Médicas; ArgentinaFil: Oliveri, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentin

Biofilms in diabetic foot ulcers: impact, risk factors and control strategies

Diabetic foot ulcers (DFUs) are a serious complication from diabetes mellitus, with a huge economic, social and psychological impact on the patients life. One of the main reasons why DFUs are so difficult to heal is related to the presence of biofilms. Biofilms promote wound inflammation and a remarkable lack of response to host defences/treatment options, which can lead to disease progression and chronicity. In fact, appropriate treatment for the elimination of these microbial communities can prevent the disease evolution and, in some cases, even avoid more serious outcomes, such as amputation or death. However, the detection of biofilm-associated DFUs is difficult due to the lack of methods for diagnostics in clinical settings. In this review, the current knowledge on the involvement of biofilms in DFUs is discussed, as well as how the surrounding environment influences biofilm formation and regulation, along with its clinical implications. A special focus is also given to biofilm-associated DFU diagnosis and therapeutic strategies. An overview on promising alternative therapeutics is provided and an algorithm considering biofilm detection and treatment is proposed.This work was supported by: Base Funding—UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy—LEPABE—funded by national funds through the FCT/MCTES (PIDDAC); Project Biocide_for_Biofilm-PTDC/BII-BTI/30219/2017- POCI-01-0145-FEDER-030219, ABFISH–PTDC/ASP-PES/28397/2017-POCI-01-0145-FEDER- 028397 and Germirrad-POCI-01-0247-FEDER-072237, funded by FEDER funds through COMPETE2020— Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PID DAC) through FCT/MCTES. The authors also thank the CITAB (Centre for the Research and Technol ogy of Agro-Environmental and Biological Sciences) under the scope of the FCT funds with reference UIDB/AGR/04033/2020. Ana Afonso (2020.04773.BD) and Diana Oliveira (SFRH/BD/138217/2018) acknowledge the FCT grants. Anabela Borges thanks the FCT for the financial support of their work contract through the Scientific Employment Stimulus—Individual Call—[CEECIND/01261/2017].info:eu-repo/semantics/publishedVersio

GAMBARAN PERAN PENGAWAS MENELAN OBAT (PMO) PADA KELUARGA DENGAN TBC PARU DI WILAYAH KERJA PUSKESMAS KOTA ATAMBUA DAN PUSKESMAS HALIWEN

Pendahuluan: Tuberculosis disebabkan oleh kuman mycobakterium tuberculosis. Pengobatan TB paru memerlukan waktu yang cukup panjang yaitu selama 6 bulan, sehingga salah satu faktor yang dapat mempengaruhi keberhasilan pengobatan tuberculosis adalah peran pengawas menelan obat (PMO). Tujuan: penelitian ini bertujuan untuk mengetahui gambaran peran pengawas menelan obat (PMO) pada anggota keluarga dengan TBC paru di Wilayah kerja Puskesmas Kota Atambua dan Puskesma Haliwen. Metode penelitian: Penelitian ini adalah penelitian deskriptif yang dilakukan di Puskesmas Kota Atambua dan Puskesmas Haliwen pada tanggal 11 November – 25 November 2022. Sampel diambil menggunakan total sampling dengan total responden sebanyak 30 PMO yang tersebar di Wilayah kerja Puskesmas Kota Atambua dan Puskesmas Haliwen. Hasil: Hasil penelitian menunjukan bahwa 83.3% PMO berhasil menjalankan peran sebagai PMO, 16% tidak berhasil menjalankan peran sebagai PMO. Kesimpulan: Hasil penelitian menunjukan bahwa sebagian besar subjek penelitian berhasil melakukan peran sebagai PMO

A experiência de aprendizagem baseada em projetos interdisciplinares em um novo campus de engenharia sob a perspectiva dos discentes

This work aims to describe the experience of Project Based Learning (PBL) in the course of Mechanical Engineering (Engineering Campus - UACSA) of the Federal Rural University of Pernambuco (UFRPE), Brazil. To this end, it were considered both the perceptions of the students and also the data analysis, collected from 20 students, about the experience. The questionnaire answered by the students was organized around six topics: theme of the project, developed competences and learning skills, teamwork, role of teachers, the project assessment and project as a teaching-learning methodology. Also they wrote on the positive and less positive aspects and pointed suggestions for the implementation of PBL. Concisely, the survey results revealed that teamwork, the need for interpersonal relationships and the development of soft skills (autonomy, initiative and creativity in creating the prototype, research capacity, decision, organization, problem solving, time management) are prominent positive aspects, added to the fact that PBL brings the chance for the students to put into practice the theoretical knowledge acquired during the course. Problems for the project's implementation were identified as: lack of instruments and conditions given to students for the development of the prototype, the dense load of disciplines of the semester, the non-suitability of some disciplines involved in the project, plus the bad interaction between them. Besides the fact that there was the need for greater dialogue between the disciplines of the semester and/or a more substantial adjustment of the Pedagogical Course Project (PPC), the PBL as a teaching-learning methodology in the course of Mechanical Engineering met the main expectations, and also collaborated for integration, socialization and learning of students. Therefore it is defended its permanence and expansion (over all semesters of the course) at UACSA/ UFRPE.O presente artigo aborda a primeira experiência da Unidade Acadêmica do Cabo de Santo Agostinho campus das engenharias da UFRPE com o trabalho interdisciplinar através da metodologia de Aprendizagem Baseada em Projetos Interdisciplinares -PBL. Para tal, foi utilizado ao final do semestre letivo um questionário voltado aos discentes, com o objetivo de avaliar a experiência em suas várias dimensões. Os resultados da pesquisa presente neste artigo baseiam-se, sobretudo, nas percepções dos estudantes sobre o processo de implementação e avaliação desta experiência. O questionário respondido pelos estudantes foi organizado em torno de seis tópicos: tema do projeto, aprendizagens e competências desenvolvidas, trabalho em equipe, papel dos docentes, avaliação no projeto e projeto como metodologia de ensino-aprendizagem. Também responderam de forma dissertativa sobre os aspetos positivos e negativos e apontaram sugestões para a implementação do PBL. A investigação realizada através do questionário revela que o trabalho em equipe, o exercício do relacionamento interpessoal e o desenvolvimento de competências transversais são aspectos positivos de destaque, somados ao fato do PBL trazer possibilidade para que os discentes coloquem em prática os conhecimentos teóricos adquiridos durante o curso, promovendo o interesse dos discentes pelo próprio aprendizado ao terem contato com a aplicabilidade dos conceitos aprendidos no curso, além de desenvolver nos mesmos um senso crítico. Somando-se a isto, também foram apontadas algumas dificuldades, principalmente no que diz respeito à articulação entre as disciplinas e a carência de instrumentos para o desenvolvimento dos protótipos. No entanto, embora esses aspectos tenham sido verificados, percebe-se que o uso do PBL como metodologia de ensino cumpre o seu papel de atender aos objetivos propostos para uma aprendizagem ativa e o desenvolvimento de sujeitos críticos e reflexivos(undefined)info:eu-repo/semantics/publishedVersio

Eficácia dos antibióticos: a perceção do usuário das regiões do Vale do Ave e de Aveiro

Os antibióticos são um grupo de drogas de extrema importância na luta contra inúmeras infeções. No entanto, a antibiótico-terapia em grande escala proporciona uma forte pressão seletiva sobre as bactérias, o que aumenta a incidência da resistência aos antibióticos e pode, a longo prazo, comprometer sua eficácia e utilidade. Uma das principais causas de resistência aos antibióticos é o tratamento inadequado.Este trabalho é financiado por: Fundos Europeus Estruturais e de Investimento, na sua componente FEDER, através do Programa Operacional Competitividade e Internacionalização (COMPETE 2020) [Projeto nº 006971 (UID/SOC/04011)]; e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia, no âmbito do projeto UID/SOC/04011/2013.info:eu-repo/semantics/publishedVersio