Volatile compounds of vegetarian soybean kapi, a fermented Thai food condiment

Kapi is a traditional shrimp paste used as a food condiment in Thailand. Several vegetarian soybean kapi, S1-S5, were fermented from various bacterial starter cultures isolated from commercial shrimp paste. The volatile compounds of S1-S5 were analyzed using SPME coupled with gaschromatography/mass spectrometry and compared to three samples of commercial vegetarian kapi (J1-J3) and commercial shrimp pastes (K1-K3). 124 volatile compounds consisting of aldehydes, alcohols,ketones, acids and esters, N-containing compounds, aromatic compounds, S-containing compounds, miscellaneous, indoles and hydrocarbons were identified. Principle component analysis and cluster analysis separated the volatile profile of the fermented samples into four groups. Vegetarian soybean kapi, S1, S4 and S5 produced from Bacillus subtilis IS4, TISTR10 and TISTR1, respectively, were classified into the following groups containing commercial kapi (J1, J2, K2 and K3), that had a predominance of indole, S-containing and N-containing compounds. Sensory evaluation of S1 showed a strong kapi odor with higher scores among the vegetarian soybean kapi and there were no significant differences in evaluation scores between S1 and commercial vegetarian kapi J1-J3. These data demonstrate that B. subtilis IS4 can be employed as a starter culture to produce an acceptable vegetarian soybean kapi substitute for shrimp paste kapi.Key words: Vegetarian kapi, volatile compounds, fermentation, principal component analysis, cluster analysis

Volatile compounds of vegetarian soybean kapi, a fermented Thai food condiment

Kapi is a traditional shrimp paste used as a food condiment in Thailand. Several vegetarian soybean kapi, S1-S5, were fermented from various bacterial starter cultures isolated from commercial shrimp paste. The volatile compounds of S1-S5 were analyzed using SPME coupled with gaschromatography/mass spectrometry and compared to three samples of commercial vegetarian kapi (J1-J3) and commercial shrimp pastes  (K1-K3). 124 volatile compounds consisting of aldehydes, alcohols, ketones, acids and esters, N-containing compounds, aromatic compounds, S-containing compounds, miscellaneous, indoles and hydrocarbons were identified. Principle component analysis and cluster analysis separated the volatile profile of the fermented samples into four groups. Vegetarian soybean kapi, S1, S4 and S5 produced from Bacillus subtilis IS4, TISTR10 and TISTR1, respectively, were classified into the following groups containing commercial kapi (J1, J2, K2 and K3), that had a predominance of indole, S-containing and N-containing compounds. Sensory evaluation of S1 showed a strong kapi odor with higher scores among the vegetarian soybean kapi and there were no significant differences in evaluation scores between S1 and commercial vegetarian kapi J1-J3. These data demonstrate that B. subtilis IS4 can be employed as a starter culture to produce an acceptable vegetarian soybean kapi substitute for shrimp paste kapi.Key words: Vegetarian kapi, volatile compounds, fermentation, principal component analysis, cluster analysis

Identifying Planetary Biosignature Impostors: Spectral Features of CO and O4 Resulting from Abiotic O2/O3 Production

O2 and O3 have been long considered the most robust individual biosignature gases in a planetary atmosphere, yet multiple mechanisms that may produce them in the absence of life have been described. However, these abiotic planetary mechanisms modify the environment in potentially identifiable ways. Here we briefly discuss two of the most detectable spectral discriminants for abiotic O2/O3: CO and O4. We produce the first explicit self-consistent simulations of these spectral discriminants as they may be seen by JWST. If JWST-NIRISS and/or NIRSpec observe CO (2.35, 4.6 um) in conjunction with CO2 (1.6, 2.0, 4.3 um) in the transmission spectrum of a terrestrial planet it could indicate robust CO2 photolysis and suggest that a future detection of O2 or O3 might not be biogenic. Strong O4 bands seen in transmission at 1.06 and 1.27 um could be diagnostic of a post-runaway O2-dominated atmosphere from massive H-escape. We find that for these false positive scenarios, CO at 2.35 um, CO2 at 2.0 and 4.3 um, and O4 at 1.27 um are all stronger features in transmission than O2/O3 and could be detected with SNRs $\gtrsim$ 3 for an Earth-size planet orbiting a nearby M dwarf star with as few as 10 transits, assuming photon-limited noise. O4 bands could also be sought in UV/VIS/NIR reflected light (at 0.345, 0.36, 0.38, 0.445, 0.475, 0.53, 0.57, 0.63, 1.06, and 1.27 um) by a next generation direct-imaging telescope such as LUVOIR/HDST or HabEx and would indicate an oxygen atmosphere too massive to be biologically produced.Comment: 7 pages, 4 figures, accepted to the Astrophysical Journal Letter

Evidence for a Dayside Thermal Inversion and High Metallicity for the Hot Jupiter WASP-18b

We find evidence for a strong thermal inversion in the dayside atmosphere of the highly irradiated hot Jupiter WASP-18b (T$_{eq}=2411K$, $M=10.3M_{J}$) based on emission spectroscopy from Hubble Space Telescope secondary eclipse observations and Spitzer eclipse photometry. We demonstrate a lack of water vapor in either absorption or emission at 1.4$\mu$m. However, we infer emission at 4.5$\mu$m and absorption at 1.6$\mu$m that we attribute to CO, as well as a non-detection of all other relevant species (e.g., TiO, VO). The most probable atmospheric retrieval solution indicates a C/O ratio of 1 and a high metallicity (C/H=$283^{+395}_{-138}\times$ solar). The derived composition and T/P profile suggest that WASP-18b is the first example of both a planet with a non-oxide driven thermal inversion and a planet with an atmospheric metallicity inconsistent with that predicted for Jupiter-mass planets at $>2\sigma$. Future observations are necessary to confirm the unusual planetary properties implied by these results

z'-band Ground-Based Detection of the Secondary Eclipse of WASP-19b

We present the ground-based detection of the secondary eclipse of the transiting exoplanet WASP-19b. The observations were made in the Sloan z'-band using the ULTRACAM triple-beam CCD camera mounted on the NTT. The measurement shows a 0.088\pm0.019% eclipse depth, matching previous predictions based on H- and K-band measurements. We discuss in detail our approach to the removal of errors arising due to systematics in the data set, in addition to fitting a model transit to our data. This fit returns an eclipse centre, T0, of 2455578.7676 HJD, consistent with a circular orbit. Our measurement of the secondary eclipse depth is also compared to model atmospheres of WASP-19b, and is found to be consistent with previous measurements at longer wavelengths for the model atmospheres we investigated.Comment: 20 pages, 10 figures. Published in the ApJ Supplement serie

Transit confirmation and improved stellar and planet parameters for the super-Earth HD 97658 b and its host star

Super-Earths transiting nearby bright stars are key objects that simultaneously allow for accurate measurements of both their mass and radius, providing essential constraints on their internal composition. We present here the confirmation, based on Spitzer transit observations, that the super-Earth HD 97658 b transits its host star. HD 97658 is a low-mass ($M_*=0.77\pm0.05\,M_{\odot}$) K1 dwarf, as determined from the Hipparcos parallax and stellar evolution modeling. To constrain the planet parameters, we carry out Bayesian global analyses of Keck-HIRES radial velocities, and MOST and Spitzer photometry. HD 97658 b is a massive ($M_P=7.55^{+0.83}_{-0.79} M_{\oplus}$) and large ($R_{P} = 2.247^{+0.098}_{-0.095} R_{\oplus}$ at 4.5 $\mu$m) super-Earth. We investigate the possible internal compositions for HD 97658 b. Our results indicate a large rocky component, by at least 60% by mass, and very little H-He components, at most 2% by mass. We also discuss how future asteroseismic observations can improve the knowledge of the HD 97658 system, in particular by constraining its age. Orbiting a bright host star, HD 97658 b will be a key target for coming space missions TESS, CHEOPS, PLATO, and also JWST, to characterize thoroughly its structure and atmosphere.Comment: 8 figures, accepted to Ap

The Galactic Exoplanet Survey Telescope (GEST)

The Galactic Exoplanet Survey Telescope (GEST) will observe a 2 square degree field in the Galactic bulge to search for extra-solar planets using a gravitational lensing technique. This gravitational lensing technique is the only method employing currently available technology that can detect Earth-mass planets at high signal-to-noise, and can measure the frequency of terrestrial planets as a function of Galactic position. GEST's sensitivity extends down to the mass of Mars, and it can detect hundreds of terrestrial planets with semi-major axes ranging from 0.7 AU to infinity. GEST will be the first truly comprehensive survey of the Galaxy for planets like those in our own Solar System.Comment: 17 pages with 13 figures, to be published in Proc. SPIE vol 4854, "Future EUV-UV and Visible Space Astrophysics Missions and Instrumentation

HST hot Jupiter transmission spectral survey: evidence for aerosols and lack of TiO in the atmosphere of WASP-12b

We present HST optical transmission spectra of the transiting hot Jupiter WASP-12b, taken with the STIS instrument. From the transmission spectra, we are able to decisively rule out prominent absorption by TiO in the exoplanet's atmosphere. Strong pressure-broadened Na and K absorption signatures are also excluded, as are significant metal-hydride features. We compare our combined broadband spectrum to a wide variety of existing aerosol-free atmospheric models, though none are satisfactory fits. However, we do find that the full transmission spectrum can be described by models which include significant opacity from aerosols: including Rayleigh scattering, Mie scattering, tholin haze, and settling dust profiles. The transmission spectrum follows an effective extinction cross section with a power-law of index alpha, with the slope of the transmission spectrum constraining the quantity alphaT = -3528+/-660 K, where T is the atmospheric temperature. Rayleigh scattering (alpha=-4) is among the best fitting models, though requires low terminator temperatures near 900 K. Sub-micron size aerosol particles can provide equally good fits to the entire transmission spectrum for a wide range of temperatures, and we explore corundum as a plausible dust aerosol. The presence of atmospheric aerosols also helps to explain the modestly bright albedo implied by Spitzer observations, as well as the near black body nature of the emission spectrum. Ti-bearing condensates on the cooler night-side is the most natural explanation for the overall lack of TiO signatures in WASP-12b, indicating the day/night cold-trap is an important effect for very hot Jupiters. These finding indicate that aerosols can play a significant atmospheric role for the entire wide range of hot-Jupiter atmospheres, potentially affecting their overall spectrum and energy balance.(abridged)Comment: 19 pages, 14 figures, 5 tables. Accepted for publication in MNRA

Gas Absorption in the KH 15D System: Further Evidence for Dust Settling in the Circumbinary Disk

Na I D lines in the spectrum of the young binary KH 15D have been analyzed in detail. We find an excess absorption component that may be attributed to foreground interstellar absorption, and to gas possibly associated with the solids in the circumbinary disk. The derived column density is log N_NaI = 12.5 cm^-2, centered on a radial velocity that is consistent with the systemic velocity. Subtracting the likely contribution of the ISM leaves log N_NaI ~ 12.3 cm^-2. There is no detectable change in the gas column density across the "knife edge" formed by the opaque grain disk, indicating that the gas and solids have very different scale heights, with the solids being highly settled. Our data support a picture of this circumbinary disk as being composed of a very thin particulate grain layer composed of millimeter-sized or larger objects that are settled within whatever remaining gas may be present. This phase of disk evolution has been hypothesized to exist as a prelude to the formation of planetesimals through gravitational fragmentation, and is expected to be short-lived if much gas were still present in such a disk. Our analysis also reveals the presence of excess Na I emission relative to the comparison spectrum at the radial velocity of the currently visible star that plausibly arises within the magnetosphere of this still-accreting young star.Comment: Accepted for publication in ApJ, 23 pages, 6 figure