INFERRING EVOLUTIONARY RELATIONSHIPS AMONG DIFFERENT HUMAN PAPILLOMAVIRUS GENOTYPES FROM MULTIPLE ALIGNMENTS OF THE MAJOR CAPSID PROTEIN L1

The major capsid protein L1 constitutes the entire exterior surface of the stabilized mature human papillomavirus (HPV), mediating initial attachment to host tissues or cells, and become pliable enough to ultimately allow release of the viral genome into a new target cell. The purpose of this study was to infer evolutionary relationships among different variable-risk HPV genotypes from comparative alignments of multiple sequences of the protein L1 deposited previously in biological information database. First, sequences of the protein L1 of 20 HPV genotypes were searched and selected from a non-redundant protein sequence database UniProtKB/Swiss-Prot. Next, a phylogenetic dendogram was constructed by comparing multiple sequences of the protein L1 using molecular evolutionary genetics analyses by Mega software. The dendogram generated from comparative alignments of the L1 protein sequences of different HPV types revealed the presence of two main clusters: a first cluster containing 12 HPV types linked intimately in several sub-branches and a second cluster grouping 8 HPV types linked in another sub-branches. Evolutionary groupings generated from L1 capsid protein sequences of variable-risk HPV genotypes demonstrated weak association between pathogenicity and phylogenetic proximity in the types analyzed, accompanied by low identity among their amino acid residues. The findings described herein reveal important insights into evolutionary patterns and phylogenetic relationships among variable-risk HPV genotypes for malignant conversion of virally infected cells from multiple alignments of the major viral capsid protein L1

The compound paraquat dichloride hydrate significantly affects the in vitro growth rate of a Chromobacterium violaceum wild type strain

Introduction: the toxicity of pesticides on bacterial cell growth is still limited. Objectives: The current study aimed to assess the in vitro growth rate of the C. violaceum wild type strain ATCC12472 exposed to the herbicide paraquat dichloride hydrate at different incubation times and final concentrations. Methodology: bacterial inocula were incubated in a nutrient broth medium containing the compound paraquat at final concentrations 100 and 1.000 µg mL-1 under aeration conditions. Spectrophotometric readings at different incubation times were carried out to estimate the in vitro bacterial growth rate. Moreover, the number of viable bacteria cells in the samples was also estimated in the presence of the paraquat at two concentrations tested based on colony-forming units grown on the nutrient broth agar. Results: significant decreases in the C. violaceum growth rate were detected, after one hour of paraquat exposure at a final concentration of 1,000 µg mL-1 (p<0.05) compared to all treatments tested. After two hours of paraquat exposure, significant decreases were progressively found at all final concentrations of 100 (p<0.01) and of 1,000 µg mL-1 (p<0.001). These data were also corroborated by counting the total number of colony-forming units at final concentrations tested. Conclusion: the findings described in current study suggest that the compound paraquat dichloride hydrate exerts significant effects on the in vitro growth rate of a C. violaceum wild type strain

Implementación de un sistema de gestión ambiental basado en la Norma ISO 14001:2015 para la reducción de impactos ambientales en la empresa Ditser S.A.C. - 2022

La presente investigación tuvo como objetivo principal implementar el Sistema de Gestión Ambiental basado en la norma ISO 14001:2015 para la reducción de los impactos ambientales en la empresa DITSER S.A.C. en oficinas administrativas y proyecto, la metodología de investigación es de tipo aplicada, de diseño experimental, apoyado de las técnicas de revisión documental y observación, usando como instrumentos listas de verificación, ficha de identificación de aspectos ambientales y matriz de medidas de control. Los resultados demostraron que DITSER S.A.C. mediante el diagnóstico cumplía con el 23.33% de los requisitos normativos de la ISO 14001:2015 y en requisitos legales cumplían con el 50%. Además, el análisis de aspectos ambientales antes y después de la implementación demostraron que el consumo de agua se redujo en 21.88%, consumo de energía eléctrica en 27.85%, generación de residuos sólidos no peligrosos en 10.75%, generación de residuos sólidos peligrosos en 34.27% y generación de ruidos molestos en 8.60%. Además, en la evaluación de la efectividad de las medidas de control, un 83% obtuvieron una valoración alta y 17% media. Concluyendo que la implementación del Sistema de Gestión Ambiental basado en la norma ISO 14001:2015 permite reducir los impactos ambientales en la empresa DITSER S.A.C

A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model

Proteoglycans encompass a heterogeneous group of glycoconjugates where proteins are substituted with linear, highly negatively charged glycosaminoglycan chains. Sulphated glycosaminoglycans are ubiquitous to the animal kingdom of the Eukarya domain. Information on the distribution and characterisation of proteoglycans in invertebrate tissues is limited and restricted to a few species. By the use of multidimensional protein identification technology and immunohistochemistry, this study shows for the first time the presence and tissue localisation of different proteoglycans, such as perlecan, aggrecan, and heparan sulphate proteoglycan, amongst others, in organs of the gastropoda Achatina fulica. Through a proteomic analysis of Golgi proteins and immunohistochemistry of tissue sections, we detected the machinery involved in glycosaminoglycan biosynthesis, related to polymer formation (polymerases), as well as secondary modifications (sulphation and uronic acid epimerization). Therefore, this work not only identifies both the proteoglycan core proteins and glycosaminoglycan biosynthetic enzymes in invertebrates but also provides a novel method for the study of glycosaminoglycan and proteoglycan evolution. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)NIHUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniv Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79912 USAUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilNIH: 2G12RR008124-16A1NIH: 2G12RR008124-16A1S1Web of Scienc

Levantamento preliminar das espécies de Lepidoptera encontradas no Campus da Fiocruz, Manguinhos (RJ), Brasil

Levantamento preliminar das espécies de Lepidoptera encontradas no Campus da Fiocruz, Manguinhos (RJ), Brasil

Analysis of predictive biomarkers in patients with lung adenocarcinoma from Southern Brazil reveals a distinct profile from other regions of the country

PURPOSE Adenocarcinoma is the most common histologic subtype of non–small-cell lung cancer, representing 40% of all diagnoses. Several biomarkers are currently used to determine patient eligibility for targeted treatments, including analysis of molecular alterations in EGFR and ALK, as well as programmed death-ligand 1 (PD-L1) protein expression. Epidemiologic data reporting the frequency of these biomarkers in Brazilian patients with lung adenocarcinoma (LUAD) are limited, and existing studies predominantly included patients from the southeast region of the country. MATERIALS AND METHODS The goal of this study was to investigate the frequency of somatic mutations in the EGFR, KRAS, NRAS, and BRAF genes, ALK, and PD-L1 expression in a series of Brazilian patients diagnosed with LUAD predominantly recruited from centers in southern Brazil. Molecular analysis of the EGFR, KRAS, NRAS, and BRAF genes was performed by next-generation sequencing using DNA extracted from tumor tissue. Immunohistochemistry was used to detect ALK and PD-L1 expression. RESULTS Analysis of 619 tumors identified KRAS mutations in 189 (30.2%), EGFR mutations in 120 (19.16%), and BRAF mutations in 19 (3%). Immunohistochemistry demonstrated ALK and PD-L1 expression in 4% and 35.1% of patients, respectively. CONCLUSION To our knowledge, this is the first study investigating the molecular epidemiology of patients with LUAD from southern Brazil and the largest assessing the frequency of multiple predictive biomarkers for this tumor in the country. The study also reveals a distinct mutation profile compared with data originating from other regions of Brazil

Saúde, corpo e gênero : perspectivas teóricas e etnográficas

-A coletânea Saúde, corpo e gênero: perspectivas teóricas e etnográficas tem como objetivo apresentar pesquisas recentes neste campo, articulando a problemática clássica em torno dos conceitos de corpo e saúde na Antropologia a questões contemporâneas, sobretudo na interface com os estudos sobre ciência, política e gênero. A partir de contextos etnográficos diversos, apresentamos três debates: o campo da enfermagem como objeto de reflexão antropológica, as amplas conexões entre gênero e saúde e a saúde indígena durante a pandemia de Covid-19. Em comum, aparece o corpo – seja um corpo submetido a dores, privações, seja um corpo que elabora ensinamentos e/ou práticas de poder específicas. O corpo torna-se um problema teórico central, aparecendo, por vezes, como questão ética para a escrita etnográfica. Destaca-se, ainda, a intensidade das emoções como elemento constitutivo dos diálogos e das interações cotidianas pesquisadas, além da permanente disputa de sentidos e saberes no campo da saúde

The International Fight Against Terrorism: A Colloquium on the Prospects for Further Cooperation Between the European Union and the United States

Members of the European Commission, European Parliament and Council of the EU joined colleagues and counterparts from the U.S. Congress, Department of Justice and Department of Homeland Security for a colloquium focusing on a range of transcendent issues in the international fight against terrorism, including common challenges and achievements, data mining, and the conflict between freedom of the individual and governmental measures taken to protect civil society

Otimização da extração assistida por ultrassom dos compostos antioxidantes da folha de moringa (moringa oleifera lam) usando metodologia de superfície de resposta / Optimization of ultrasover assisted extraction of the antioxidant compounds of the moringa leaf (moringa oleifera lam) using response surface methodology

A Moringa oleifera Lam é uma planta nativa da Índia. Suas folhas contêm nutrientes importantes como proteínas, vitamina C, além destes nutrientes as folhas contêm uma série de substâncias com capacidade antioxidante. Para se extrair os compostos antioxidante existe técnicas convencionais como Soxhlet e a maceração e as técnicas de extração não convencionais como extração assistido por Ultrassom. Assim, este trabalho teve como objetivo otimizar o processo de extração dos compostos antioxidantes das folhas da moringa. A otimização da extração, foi realizado por meio do planejamento fatorial 3², utilizando como variáveis independentes a concentração de solvente e o tempo de extração e como variáveis dependentes a atividade antioxidante frente ao radical ABTS e ao potencial redutor de ferro (FRAP). O extrato que apresentou maior atividade antioxidante pelos métodos ABTS e FRAP foi o obtido com etanol 70% (%v/v), na extração por ultrassom e agitação mecânica durante 30 min de extração