9,523 research outputs found
Commensal-derived OMVs elicit a mild proinflammatory response in intestinal epithelial cells
Under normal physiological conditions, the intestinal immunity remains largely hyporesponsive to the commensal microbiota, yet also retains the inherent ability to rapidly respond to pathogenic antigens. However, immunomodulatory activities of extracellular products from commensal bacteria have been little studied, with previous investigations generally utilising the live bacterium to study microbiota-epithelial interactions. In this study, we demonstrate that extracellular products of a commensal bacterium, Escherichia coli C25, elicit a moderate release of proinflammatory IL-8 and stimulate transcriptional up-regulation of Toll-like receptors (TLRs) in intestinal epithelial cell lines, HT29-19A and Caco-2. Additionally, we show that removal of outer membrane vesicles (OMVs) diminishes the proinflammatory effect of secreted products from E. coli C25. Furthermore, we show that isolated OMVs have a dose-dependent proinflammatory effect on IECs. Interestingly, a relatively high concentration (10x culture concentration) of OMVs had no significant regulatory effects on TLR mRNA expression in both cell lines. Finally, we also demonstrate a that pre-incubation with E. coli C25-derived OMVs subsequently inhibited the internalisation of the bacterium itself in both cell lines. Taken together, our results suggest that commensal-derived extracellular products, in particular OMVs, could significantly contribute to intestinal homeostasis. We also demonstrate a unique interaction between commensal-derived OMVs and host cells
Measuring fast electron spectra and laser absorption in relativistic laser-solid interactions using differential bremsstrahlung photon detectors
A photon detector suitable for the measurement of bremsstrahlung spectra
generated in relativistically-intense laser-solid interactions is described.
The Monte Carlo techniques used to back-out the fast electron spectrum and
laser energy absorbed into fast electrons are detailed. A
relativistically-intense laser-solid experiment using frequency doubled laser
light is used to demonstrate the effective operation of the detector. The
experimental data was interpreted using the 3-spatial-dimension Monte Carlo
code MCNPX (Pelowitz 2008), and the fast electron temperature found to be 125
keV
A Randomized Controlled Trial of Interleukin-1 Receptor Antagonist in a Rabbit Model of Ascending Infection in Pregnancy
Objective: To determinewhether treatment with interleukin-1 receptor antagonist (IL1-ra) would affect amniotic fluid concentrations of tumor necrosis factor alpha (TNF-α) and prostaglandins or clinical or microbiological outcomes in a model of ascending bacterial infection in pregnancy. Methods: Timed pregnant New Zealand white rabbits at 70% of gestation underwent endoscopic inoculation of the cervices with 10(6)–10(6) cfu Escherichia coli . Animals were randomly assigned in a blinded manner to a 5-h intravenous infusion of human IL1-ra (10 mg/kg) or placebo beginning 1 – 2 h after inoculation. Blood was drawn fromthe does for assay of serum IL1-ra concentration before inoculation, at mid-infusion, after the infusion ended and at necropsy. At necropsy, temperature and cultures were taken, and aspirated amniotic fluid was pooled for assays of TNF-α, prostaglandin E(2) (PGE(2)) and IL1-ra. Results: Serum IL1-ra concentrations rose to a mean of 2 mg/ml at mid-infusion and fell markedly after the infusion to concentrations barely detectable at necropsy. Between the two groups, there were no significant differences in the rates of fever or positive cultures or in amniotic fluid concentrations of PGE(2) or TNF-α.One unique finding was the demonstration that administration of human IL1-ra to the does resulted inmeasurable concentrationsof human IL1-ra in the amniotic fluid. Conclusions: Treatment with an intravenous infusion of human IL1-ra after cervical inoculation with E. coli did not affect clinical or microbiological outcomes or amniotic fluid concentrations of TNF-α or PGE(2). This experiment provides the first demonstration of passageof human IL1-ra from the maternal bloodstreamto the amniotic fluid
Radio source calibration for the VSA and other CMB instruments at around 30 GHz
Accurate calibration of data is essential for the current generation of CMB
experiments. Using data from the Very Small Array (VSA), we describe procedures
which will lead to an accuracy of 1 percent or better for experiments such as
the VSA and CBI. Particular attention is paid to the stability of the receiver
systems, the quality of the site and frequent observations of reference
sources. At 30 GHz the careful correction for atmospheric emission and
absorption is shown to be essential for achieving 1 percent precision. The
sources for which a 1 percent relative flux density calibration was achieved
included Cas A, Cyg A, Tau A and NGC7027 and the planets Venus, Jupiter and
Saturn. A flux density, or brightness temperature in the case of the planets,
was derived at 33 GHz relative to Jupiter which was adopted as the fundamental
calibrator. A spectral index at ~30 GHz is given for each. Cas A,Tau A, NGC7027
and Venus were examined for variability. Cas A was found to be decreasing at
percent per year over the period March 2001 to August 2004.
In the same period Tau A was decreasing at percent per year. A
survey of the published data showed that the planetary nebula NGC7027 decreased
at percent per year over the period 1967 to 2003. Venus showed
an insignificant ( percent) variation with Venusian illumination.
The integrated polarization of Tau A at 33 GHz was found to be
percent at pa .}Comment: 13 pages, 15 figures, submitted to MNRA
Further Sunyaev-Zel'dovich observations of two Planck ERCSC clusters with the Arcminute Microkelvin Imager
We present follow-up observations of two galaxy clusters detected blindly via
the Sunyaev-Zel'dovich (SZ) effect and released in the Planck Early Release
Compact Source Catalogue. We use the Arcminute Microkelvin Imager, a dual-array
14-18 GHz radio interferometer. After radio source subtraction, we find a SZ
decrement of integrated flux density -1.08+/-0.10 mJy toward PLCKESZ
G121.11+57.01, and improve the position measurement of the cluster, finding the
centre to be RA 12 59 36.4, Dec +60 04 46.8, to an accuracy of 20 arcseconds.
The region of PLCKESZ G115.71+17.52 contains strong extended emission, so we
are unable to confirm the presence of this cluster via the SZ effect.Comment: 4 tables, 3 figures, revised after referee's comments and resubmitted
to MNRA
A VSA search for the extended Sunyaev-Zel'dovich Effect in the Corona Borealis Supercluster
We present interferometric imaging at 33 GHz of the Corona Borealis
supercluster, using the extended configuration of the Very Small Array. A total
area of 24 deg^2 has been imaged, with an angular resolution of 11 arcmin and a
sensitivity of 12 mJy/beam. The aim of these observations is to search for
Sunyaev-Zel'dovich (SZ) detections from known clusters of galaxies in this
supercluster and for a possible extended SZ decrement due to diffuse warm/hot
gas in the intercluster medium. We measure negative flux values in the
positions of the ten richest clusters in the region. Collectively, this implies
a 3.0-sigma detection of the SZ effect. In the clusters A2061 and A2065 we find
decrements of approximately 2-sigma. Our main result is the detection of two
strong and resolved negative features at -70+-12 mJy/beam (-157+-27 microK) and
-103+-10 mJy/beam (-230+-23 microK), respectively, located in a region with no
known clusters, near the centre of the supercluster. We discuss their possible
origins in terms of primordial CMB anisotropies and/or SZ signals related to
either unknown clusters or to a diffuse extended warm/hot gas distribution. Our
analyses have revealed that a primordial CMB fluctuation is a plausible
explanation for the weaker feature (probability of 37.82%). For the stronger
one, neither primordial CMB (probability of 0.33%) nor SZ can account alone for
its size and total intensity. The most reasonable explanation, then, is a
combination of both primordial CMB and SZ signal. Finally, we explore what
characteristics would be required for a filamentary structure consisting of
warm/hot diffuse gas in order to produce a significant contribution to such a
spot taking into account the constraints set by X-ray data.Comment: 16 pages, 10 figures. Accepted in MNRA
The ATLAS3D project - XXV: Two-dimensional kinematic analysis of simulated galaxies and the cosmological origin of fast and slow rotators
We present a detailed two-dimensional stellar dynamical analysis of as ample of 44 cosmological hydrodynamical simulations of individual central galaxies with stellar masses of 2 x 1010Msun ∼≤ Mstar ∼≤ 6x 1011Msun. Kinematic maps of the stellar line-of-sight velocity, velocity dispersion, and higher-order Gauss-Hermite moments h3 and h4 are constructed for each central galaxy and for the most massive satellites. The amount of rotation is quantified using the λR-parameter. The velocity, velocity dispersion, h3, and h4 fields of the simulated galaxies show a diversity similar to observed kinematic maps of early-type galaxies in the ATLAS3D survey. This includes fast (regular), slow, and misaligned rotation, hot spheroids with embedded cold disk components as well as galaxies with counter-rotating cores or central depressions in the velocity dispersion. We link the present-day kinematic properties to the individual cosmological formation histories of the galaxies. In general, major galaxy mergers have a significant influence on the rotation properties resulting in both a spin-down as well as a spin-up of the merger remnant. Lower mass galaxies with significant in-situ formation of stars, or with additional gas-rich major mergers - resulting in a spin-up - in their formation history, form elongated fast rotators with a clear anti-correlation of h3 and v/σ. An additional formation path for fast rotators includes gas-poor major mergers leading to a spin-up of the remnants. This formation path does not result in anti-correlated h3 and v/σ. The galaxies most consistent with the rare class of non-rotating round early-type galaxies grow by gas-poor minor mergers alone. In general, more massive galaxies have less in-situ star formation since z ∼ 2, rotate slower and have older stellar populations. (shortened)PostprintPeer reviewe
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