EXTH-08. REPLACEMENT OF MICROGLIA BY BRAIN-ENGRAFTED MACROPHAGES PREVENTS MEMORY DEFICITS AFTER THERAPEUTIC WHOLE-BRAIN IRRADIATION

Abstract Microglia have a distinct origin compared to blood circulating myeloid cells. Under normal physiological conditions, microglia are maintained by self-renewal, independent of hematopoietic progenitors. Following genetic or pharmacologic depletion, newborn microglia derive from the local residual pool and quickly repopulate the entire brain. The depletion of brain resident microglia during therapeutic whole-brain irradiation fully prevents irradiation-induced synaptic loss and recognition memory deficits but the mechanisms driving these protective effects are unknown. Here, we demonstrate that after CSF-1R inhibitor-mediated microglia depletion and therapeutic whole-brain irradiation, circulating monocytes engraft into the brain and replace the microglia pool. These monocyte-derived brain-engrafted macrophages have reduced phagocytic activity compared to microglia from irradiated brains, but similar to locally repopulated microglia without brain irradiation. Transcriptome comparisons reveal that brain-engrafted macrophages have both monocyte and embryonic microglia signatures. These results suggest that monocyte-derived brain-engrafted macrophages represent a novel therapeutic avenue for the treatment of brain radiotherapy-induced cognitive deficits

Rekayasa dan Pengujian Performansi Isolator Polimer Resin Epoksi Dengan Filler Fly Ash

Pada penelitian ini isolator terbuat dari bahan resin epoksi dengan filler fly ash dan aluminium oksida dan divariasikan dengan 2 tipe sirip isolator yaitu BBB dan BKB dengan pembuatan dimensi yang memperhatikan standar IEC. Pengujian yang dilakukan menggunakan 2 kondisi untuk masing-masing isolator yaitu kondisi kering dan kondisi basah. Pada penelitian ini akan diuraikan pengaruh dari tipe variasi sirip permukaan isolator terhadap distribusi medan listrik, sudut kontak, tingkat arus bocor, dan tegangan flashover dalam kondisi kering dan basah. Metode penelitian yang digunakan adalah dengan mengambil data arus bocor dan tegangan flashover secara langsung di Laboratorium Teknik Tegangan Tinggi Universitas Brawijaya, faktor bentuk, debit pembasahan, sudut kontak, serta dilakukan simulasi distribusi medan listrik pada masing-masing variasi isolator menggunakan software FEMM 4.2. Pengujian dilakukan dengan menggunakan tegangan tinggi AC. Hasil yang didapat dari penelitian ini isolator tipe sirip seragam memiliki nilai arus bocor yang lebih kecil dari tipe sirip tidak seragam baik pada kondisi kering dan basah sangat berpengaruh terhadap arus bocor. Debit pembasahan juga berpengaruh terhadap arus bocor, dimana peningkatan debit pembasahan berbanding lurus dengan tingkat arus bocor dan berbanding terbalik dengan ketahanan suatu isolator menahan tegangan flashover serta kuat medan rata-rata yang paling maksimum terdapat pada isolator tipe sirip tidak seragam dan kuat medan paling rendah terdapat pada isolator tipe sirip seragam, dan sesuai dengan perhitungan faktor bentuk bahwa nilai faktor bentuk yang paling tinggi terdapat pada isolator tipe sirip seragam, serta yang paling terendah terdapat pada isolator tipe sirip tidak seragam, dan hasil perhitungan sudut kontak yang paling tertinggi terdapat pada isolator tipe sirip seragam dan yang paling terendah terdapat pada isolator tipe sirip tidak seragam sehingga sifat hidrofobik yang paling baik berada di isolator sirip seragam

Behavior of polyethylene-based nanocomposites containing multi-layer graphene filler

open4siIn this research, the results regarding the characterization of electrical and mechanical properties of a low-density polyethylene (LDPE) - graphene-like (G) nanocomposite are presented. The processing technique used to fabricate the material was high-energy ball milling. This method was utilized to fabricate samples with a filler loading ranging from 1 wt% up to 7wt%. Low voltage broadband dielectric spectroscopy (BDS), erosion tests and breakdown tests (BD) were the measurements carried out in order to characterize the electrical behavior of the fabricated composites. Then, some of the mechanical properties of the LDPE-G nanocomposites were determined with dynamic mechanical analysis (DMA) and, finally, the crystalline structure of the material was investigated with differential scanning calorimetry (DSC).embargoed_20200114Rosi, G.F.; Frechette, M.; David, E.; Fabiani, D.Rosi, G.F.; Frechette, M.; David, E.; Fabiani, D

Influence of feedstock and operational conditions on bio-chars derived from the pyrolysis of selected biomasses

The proprieties of bio-char, the solid product from biomass pyrolysis, depends on both the feedstock and process conditions during thermochemical conversion[1]. As regards the interaction of the char with soil (i.e. as soil amendment), surface areas, size and shape of pores are among the most important factors to be considered. [1] P. R. Bonelli , G. Nunell , M. E. Fernández , E. L. Buonomo & A. L. Cukierman (2012) The Potential Applications of the Bio-char Derived from the Pyrolysis of an Agro-industrial Waste. Effects of Temperature and Acid-pretreatment, Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 34:8, 746-755, DOI: 10.1080/15567031003681937 Please click Additional Files below to see the full abstract

Experiencia Plan de Dinamización de Pomar.

Sin resume

Biochar from lab-scale pyrolysis: influence of feedstock and operational temperature

AbstractBiochar properties are highly dependent on the feedstock type and operational conditions during thermochemical processing, in particular slow pyrolysis. To clarify this aspect, nine biochars were produced by pyrolyzing in a macro TGA at 400, 550, and 650 °C three different decorticated and chopped biomasses. The studied biomasses are representative of conifer (black pine) and deciduous (poplar and willow) woods. Biochar surface area, size, and shape of pores were investigated by means of nitrogen adsorption isotherm, Hg porosimetry, and electron microscopy. The results indicate that biochars with high surface area can be obtained at high temperature, especially starting from pine feedstock. Regarding porosity, micro-pores (1–10 nm) are not remarkably affected by the starting feedstocks, while macro-pores (> 10 nm) are strictly connected with the morphology of the starting wood. More than the surface area, we found a strong correlation between the chemical composition (elemental composition and FTIR) of the biochars and their retention and release capacity of ions (cation exchange capacity, CEC). The trend in the CEC, determined via coupled approach by spectrophotometric and ion chromatography, reveals that the increase in the processing temperature has the effect of reducing the number of functional groups able of exchanging the cations with the equilibrium solution. This work represents a step forward in the characterization of the char produced by pyrolysis of biomass thanks to the development of a multi-technique approach allowing to obtain a structure-property correlation of the biochars. Our results and experimental approach can help in the optimization of the parameters used in the preparation of these materials.Graphical abstrac

Long‐term research reveals multiple relationships between the abundance and impacts of a non‐native species

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147876/1/lno11029.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147876/2/lno11029_am.pd

TNF-α protein synthesis inhibitor restores neuronal function and reverses cognitive deficits induced by chronic neuroinflammation

Abstract Background Chronic neuroinflammation is a hallmark of several neurological disorders associated with cognitive loss. Activated microglia and secreted factors such as tumor necrosis factor (TNF)-α are key mediators of neuroinflammation and may contribute to neuronal dysfunction. Our study was aimed to evaluate the therapeutic potential of a novel analog of thalidomide, 3,6'-dithiothalidomide (DT), an agent with anti-TNF-α activity, in a model of chronic neuroinflammation. Methods Lipopolysaccharide or artificial cerebrospinal fluid was infused into the fourth ventricle of three-month-old rats for 28 days. Starting on day 29, animals received daily intraperitoneal injections of DT (56 mg/kg/day) or vehicle for 14 days. Thereafter, cognitive function was assessed by novel object recognition, novel place recognition and Morris water maze, and animals were euthanized 25 min following water maze probe test evaluation. Results Chronic LPS-infusion was characterized by increased gene expression of the proinflammatory cytokines TNF-α and IL-1β in the hippocampus. Treatment with DT normalized TNF-α levels back to control levels but not IL-1β. Treatment with DT attenuated the expression of TLR2, TLR4, IRAK1 and Hmgb1, all genes involved in the TLR-mediated signaling pathway associated with classical microglia activation. However DT did not impact the numbers of MHC Class II immunoreactive cells. Chronic neuroinflammation impaired novel place recognition, spatial learning and memory function; but it did not impact novel object recognition. Importantly, treatment with DT restored cognitive function in LPS-infused animals and normalized the fraction of hippocampal neurons expressing the plasticity-related immediate-early gene Arc. Conclusion Our data demonstrate that the TNF-α synthesis inhibitor DT can significantly reverse hippocampus-dependent cognitive deficits induced by chronic neuroinflammation. These results suggest that TNF-α is a critical mediator of chronic neuroinflammation-induced neuronal dysfunction and cognitive impairment and targeting its synthesis could provide an effective therapeutic approach to several human neurodegenerative diseases

Ring-Opening Polymerisation of rac-Lactide Using a Calix[4]arene-Based Titanium (IV) Complex

cone-25,27-Dipropyloxy-26,28-dioxo-calix[4]arene titanium (IV) dichloride(1)has been assessed in the ring-opening polymerisation ofrac-lactide (L,D-LA). The polymers formed (PLDA) turned out to display an isotactic stereoblock microstructure (determined by NMR) despite the fact that the catalyst hasC2vsymmetry. Two techniques were applied for initiating the polymerisation reaction, microwave irradiation, and conventional thermal treatment. The polymers obtained were all characterised by NMR, IR, HPLC-SEC, DSC, and MALDI-TOF analysis. The use of microwave irradiation, applied for the first time to calixarene-based catalysts in the presence of therac-lactide monomer, increased the polymerisation rate compared with that obtained by the other method. On the other hand, standard thermal treatment enabled a slightly better control than microwave irradiation over the molecular weight and molecular weight distribution of the polylactides formed