A quantitative approach of the morphological changes in the middle Garonne river (South-west France) during the last 150 years

International audienceThe aim of this study is to periodize the geomorphological evolution of the Middle Garonne river from Toulouse to the tarn junction from middle 19th century to Present, in order to highlight the respective role of climate vs anthropization as diving force

Dynamique fluviale de la Garonne à l'anthropocène : trajectoire d'évolution du tronçon fluvial compris entre les confluences de l'Ariège et du Tarn (Garonne toulousaine, 90 km)

Since the end of the nineteenth century, significant morphological channel changes have been observed along European rivers. The major processes involved, channel incision and narrowing, appear as the result of increasing human activities and the transition from the Little Ice Age (LIA) to Global Warming (GW). On the Garonne River (southwestern France) this phenomenon has been observed and described since the 1960th between the Ariège and Tarn tributaries (The Toulouse Garonne, 90 km). However, very few studies have focused on a longer evolutionary period. In this study, we propose to analyze the evolutionary trajectory of the Toulouse Garonne on a plurisecular timescale. The aim of this study is to assess: 1) the evolutionary tendency before the 1950th; 2) the impact of the LIA to GW transition and former human disturbances on fluvial dynamics; 3) the hydro-sedimentary pattern of the Garonne River before the twentieth century; and 4) the presence of spatial discontinuities within the reach. The analysis is based on two types of data, which are complementary in terms of chronology and spatial cover: historical maps and field data collected on 11 palaeochannels. The results show: 1) a meandering channel in the late LIA (second half of the nineteenth century), with spatial discontinuities along the Toulouse Garonne; 2) a morphological adjustment since the late nineteenth / early twentieth centuries in the upstream and middle reaches, in response to several controls and; 3) a later adjustment in the downstream reach, in response to bank protection. Furthermore, this study provides new data on morphological channel features over the Medieval Climatic Optimum (MCO, 900-1300) and reveals that only a very few data allows to assess the channel behavior over the LIA.Depuis la fin du XIXe siècle / le début du XXe siècle, les cours d'eau européens sont affectés par une incision et une contraction généralisées des chenaux, en réponse à l'intensification des activités humaines et à la transition climatique entre le Petit Âge Glaciaire (PAG) et le Réchauffement Global actuel (RG). Sur la Garonne, ce phénomène a été observé et décrit depuis les années 1960 dans la portion dite toulousaine (linéaire de 90 km entre les confluences de l'Ariège et du Tarn). Toutefois, peu d'études se sont intéressées à l'évolution de la dynamique fluviale à plus long terme. Cette thèse propose une reconstitution de la trajectoire d'évolution de la Garonne toulousaine sur une échelle de temps pluriséculaire. L'objectif est d'évaluer : 1) l'inscription de la tendance actuelle sur la période antérieure aux années 1950 ; 2) l'effet de la transition PAG/RG et des aménagements ponctuels de l'Epoque Moderne sur la dynamique fluviale ; 3) le mode de fonctionnement hydro-sédimentaire de la Garonne avant le XXe siècle et 4) l'existence de tendances/disparités spatiales au sein du linéaire. La démarche méthodologique mise en oeuvre repose sur la complémentarité chronologique et spatiale des données traitées, issues de l'analyse des documents cartographiques historiques et de travaux de terrain menés sur 11 paléochenaux de la Garonne. Les résultats révèlent : 1) une tendance au méandrage au cours de la phase terminale du PAG (seconde moitié du XIXe siècle), marquée par des discontinuités le long du linéaire ; 2) une réduction de la dynamique fluviale amorcée dès la fin du XIXe siècle / le début du XXe siècle dans la partie amont/médiane du linéaire, en réponse à des facteurs de contrôle multiples et 3) une réduction de la dynamique fluviale plus tardive dans la partie aval, sous l'effet d'une campagne de protection des berges. Par ailleurs, cette étude fournit des données sur la période correspondant à l'Optimum Climatique Médiéval (OM, 900-1300) et révèle une faiblesse documentaire concernant le PAG avant le XIXe siècle

Statistiques de l'enseignement supérieur /

Édition 2013

Le gisement de méga-fossiles ligneux de Bourret (moyenne vallée de la Garonne) : processus de formation et signification climatique

International audienceLe gisement de mégafossiles ligneux de Bourret est le plus important identifié à ce jour en vallée de Garonne. La datation14C de 8 des 17 troncs subfossiles de Quercus extraits s’échelonne entre 8 300 et 4 200 ans cal. BP. Elles attestent du caractèrepartiellement remanié du gisement. Certains de ces bois ont subi une ou des phases de stockage temporaire d’une durée compriseentre 4 100 et 1 000 ans avant d’être incorporés définitivement dans la basse plaine. Les bois les plus anciens ont donc connu unehistoire complexe comportant une phase d’injection dans le chenal, de stockage dans des unités intermédiaires, puis une érosion etune nouvelle phase de dépôt avec la charge de fond. Les bois les plus jeunes résultent quant à eux d’une injection directe depuis laripisylve dans le système fluvial. L’agrégation des dates obtenues sur ce site et sur les sites découverts antérieurement s’organisent en3 agrégats (env. 8 200, 5 200 et 4 200 ans cal. BP). Ceci suggère que l’injection des bois dans le système fluvial est liée à des phasesde forte activité hydrosédimentaire. Le synchronisme avec les Rapid Climate Changes (RCC) suggère un contrôle climatique sur ceprocessus, ce qui nous amène à proposer un schéma conceptuel de formation des gisements de bois fossiles

The subfossil tree deposits from the Garonne Valley and their implications on Holocene alluvial plain dynamics

International audienceSubfossil tree trunks deposits are common in large rivers, but their status as a source for dating alluvial sequences and palaeoenvironmental studies is still discussed. Particularly their origin and the process(es) of deposition as well as a possible remobilization were pointed as a limit to their use to document river alluvial changes. In this work we report the discovery of the largest subfossil trunks deposits in the Garonne valley. These new data are compared to the previous ones. A set of 17 tree trunks and more than 300 smaller wood fragments were collected. The xylologic study shows the prevalence of Quercus and a single occurrence of Ulmus. These two hardwood species are commonly associated with riparian forest. The 14C dating carried out on seven trunks and a single branch of Quercus on the outermost identified growth rings, indicates age ranging from 8400-8000 cal. BP for the oldest fragment (bough) to 4300-4000 cal. BP for the most recent tree trunk. Radiocarbon ages of the trunks are aggregated into two main periods: 5300-5600 cal. BP (four trunks) and 4300-4000 cal. BP (three trunks). The radiocarbon (charcoal) dating of the top of the alluvial sequence overlaying the trunks gives an age between 1965-1820 and 1570-1810 cal. BP, i.e. between the 2nd and the 5th c. AD. In addition, the discovery of two unpublished subfossil tree trunks deposits in Finhan are reported (six trunks). At the light of these results, we discuss previously proposed models for the Garonne floodplain building

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production

From Springer Nature via Jisc Publications RouterHistory: received 2021-03-02, accepted 2021-07-29, registration 2021-08-20, pub-electronic 2021-09-06, online 2021-09-06, collection 2021-12Publication status: PublishedFunder: EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council); doi: https://doi.org/10.13039/100010663; Grant(s): pre-FAB 695013Abstract: Isobutene is a high value gaseous alkene used as fuel additive and a chemical building block. As an alternative to fossil fuel derived isobutene, we here develop a modified mevalonate pathway for the production of isobutene from glucose in vivo. The final step in the pathway consists of the decarboxylation of 3-methylcrotonic acid, catalysed by an evolved ferulic acid decarboxylase (Fdc) enzyme. Fdc belongs to the prFMN-dependent UbiD enzyme family that catalyses reversible decarboxylation of (hetero)aromatic acids or acrylic acids with extended conjugation. Following a screen of an Fdc library for inherent 3-methylcrotonic acid decarboxylase activity, directed evolution yields variants with up to an 80-fold increase in activity. Crystal structures of the evolved variants reveal that changes in the substrate binding pocket are responsible for increased selectivity. Solution and computational studies suggest that isobutene cycloelimination is rate limiting and strictly dependent on presence of the 3-methyl group

Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens

Fungi of the Pucciniales order cause rust diseases which, altogether, affect thousands of plant species worldwide and pose a major threat to several crops. How rust effectors—virulence proteins delivered into infected tissues to modulate host functions— contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplar, and its genome encodes 1184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria, we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana. Infection assays using bacterial (Pseudomonas syringae) and oomycete (Hyaloperonospora arabidopsidis) pathogens revealed subsets of candidate effectors that enhanced or decreased pathogen leaf colonization. Confocal imaging of green fluorescent protein-tagged candidate effectors constitutively expressed in stable transgenic plants revealed that some protein fusions specifically accumulate in nuclei, chloroplasts, plasmodesmata and punctate cytosolic structures. Altogether, our analysis suggests that rust fungal candidate effectors target distinct cellular components in host cells to promote parasitic growth. © 2016 BSPP AND JOHN WILEY & SONS LTD

Low Efficiency of Homology-Facilitated Illegitimate Recombination during Conjugation in Escherichia coli

Homology-facilitated illegitimate recombination has been described in three naturally competent bacterial species. It permits integration of small linear DNA molecules into the chromosome by homologous recombination at one end of the linear DNA substrate, and illegitimate recombination at the other end. We report that homology-facilitated illegitimate recombination also occurs in Escherichia coli during conjugation with small non-replicative plasmids, but at a low frequency of 3×10−10 per recipient cell. The fate of linear DNA in E. coli is either RecBCD-dependent degradation, or circularisation by ligation, and integration into the chromosome by single crossing-over. We also report that the observed single crossing-overs are recA-dependent, but essentially recBCD, and recFOR independent. This suggests that other, still unknown, proteins may act as mediator for the loading of RecA on DNA during single crossing-over recombination in E. coli

Variability of ambient air ammonia in urban Europe (Finland, France, Italy, Spain, and the UK)

This study addressed the scarcity of NH3 measurements in urban Europe and the diverse monitoring protocols, hindering direct data comparison. Sixty-nine datasets from Finland, France, Italy, Spain, and the UK across various site types, including industrial (IND, 8), traffic (TR, 12), urban (UB, 22), suburban (SUB, 12), and regional background (RB, 15), are analyzed to this study. Among these, 26 sites provided 5, or more, years of data for time series analysis. Despite varied protocols, necessitating future harmonization, the average NH3 concentration across sites reached 8.0 ± 8.9 μg/m3. Excluding farming/agricultural hotspots (FAHs), IND and TR sites had the highest concentrations (4.7 ± 3.2 and 4.5 ± 1.0 μg/m3), followed by UB, SUB, and RB sites (3.3 ± 1.5, 2.7 ± 1.3, and 1.0 ± 0.3 μg/m3, respectively) indicating that industrial, traffic, and other urban sources were primary contributors to NH3 outside FAH regions. When referring exclusively to the FAHs, concentrations ranged from 10.0 ± 2.3 to 15.6 ± 17.2 μg/m3, with the highest concentrations being reached in RB sites close to the farming and agricultural sources, and that, on average for FAHs there is a decreasing NH3 concentration gradient towards the city. Time trends showed that over half of the sites (18/26) observed statistically significant trends. Approximately 50 % of UB and TR sites showed a decreasing trend, while 30 % an increasing one. Meta-analysis revealed a small insignificant decreasing trend for non-FAH RB sites. In FAHs, there was a significant upward trend at a rate of 3.51[0.45,6.57]%/yr. Seasonal patterns of NH3 concentrations varied, with urban areas experiencing fluctuations influenced by surrounding emissions, particularly in FAHs. Diel variation showed differing patterns at urban monitoring sites, all with higher daytime concentrations, but with variations in peak times depending on major emission sources and meteorological patterns. These results offer valuable insights into the spatio-temporal patterns of gas-phase NH3 concentrations in urban Europe, contributing to future efforts in benchmarking NH3 pollution control in urban areas.</p

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030