ベトナムダイオキシン高濃度汚染地域における授乳中の母親の唾液中ホルモン値の検討

取得学位 : 博士（保健学）, 学位授与番号 : 医博甲第2103号 , 学位授与年月日 : 平成22年3月23日, 学位授与大学 : 金沢大学, 審査結果の報告日 : 平成22年2月16

A RESEARCH ON MULTI-OBJECTIVE OPTIMIZATION OF THE GRINDING PROCESS USING SEGMENTED GRINDING WHEEL BY TAGUCHI-DEAR METHOD

In this study, the mutil-objective optimization was applied for the surface grinding process of SAE420 steel. The aluminum oxide grinding wheels that were grooved by 15 grooves, 18 grooves, and 20 grooves were used in the experimental process. The Taguchi method was applied to design the experimental matrix. Four input parameters that were chosen for each experiment were the number of grooves in cylinder surface of grinding wheel, workpiece velocity, feed rate, and cutting depth. Four output parameters that were measured for each experimental were the machining surface roughness, the system vibrations in the three directions (X, Y, Z). The DEAR technique was applied to determine the values of the input parameters to obtaine the minimum values of machining surface roughness and vibrations in three directions. By using this technique, the optimum values of grinding wheel groove number, workpiece velocity, feed-rate, cutting depth were 18 grooves, 15 m/min, 2 mm/stroke, and 0.005 mm, respectively. The verified experimental was performed by using the optimum values of input parameters. The validation results of surface roughness and vibrations in X, Y, Z directions were 0.826 (µm), 0.531 (µm), 0.549 (µm), and 0. 646 (µm), respectively. These results were great improved in comparing to the normal experimental results. Taguchi method and DEAR technique can be applied to improve the quality of grinding surface and reduce the vibrations of the technology system to restrain the increasing of the cutting forces in the grinding process. Finally, the research direction was also proposed in this stud

1H- AND 13C-NMR SPECTRA OF SOME AZOMETHINES OF 5-AMINO-2-PHENYLINDOLE SERIES

The 1H- and 13C-spectra of some azomethines of 5-amino-2-phenylindole series have been recorded and researched. It shown that, the substituents in benzene ring influenced on the chemical shift of proton on azomethine bond

Class Day Programme, May (1892)

https://red.mnstate.edu/commencement/1002/thumbnail.jp

Liquid pumping and mixing by PZT synthetic jet

In this paper, a PZT synthetic jet that can function as both an efficient pumping and mixing device is developed. Compare with the conventional design where the practice of controlling the internal flow is undertaken by microvalves structure, this approach promotes the durability and allows the device to work with different liquids at high Reynold number without losing of backflow from the diffuser, therefore provides efficient mixing. The pumping performance is applicable for commercialized counterparts while the homogeneous medium was obtained at downstream in the experiments, which was further confirmed by simulation. Notably, the chaotic mixing feature of the device is also applicable for immiscible liquids with the micro-droplet formation result at the outlet

Unexpected cases in field diagnosis of African swine fever virus in Vietnam: The needs consideration when performing molecular diagnostic tests

Background: The first confirmed case of African swine fever (ASF) in Vietnam was reported officially in February 2019. To date, ASF virus (ASFV) have been detected in 63/63 provinces in Vietnam. Currently, real-time polymerase chain reaction (PCR) is considered to be a powerful tool for viral detection in field samples, including ASFV. However, some recent reports have suggested that mismatches in primer and probe binding regions may directly affect real-time PCR qualification, leading a false-negative result.Aim: This study aims to further examine a conflicting result obtained from two OIE recommended methods, conventional PCR and real-time PCR, for ASFV detection.Methods: Two ASF suspected pigs from different provinces in the north of Vietnam were selected for this study based on clinical signs and postmortem lesions. The different results obtained by OIE-recommended conventional PCR and real-time PCR were further analyzed by the Sanger sequencing method and virus isolation in combination with hemadsorption (HAD) test using porcine alveolar macrophages cells.Results: The results showed that when the primer sequence matched perfectly with the sequences of field isolates, a mutation in probe binding region was found, indicating that a single mismatch in the probe binding site may cause a false-negative result by real-time PCR in detecting ASFV in clinical samples in Vietnam. An agreement between conventional PCR, using PPA1/PPA2 primers and two golden standard methods, virus isolation in combination with HAD assay, and sequencing method was observed in this study.Conclusion: A single mismatch in the probe binding site caused a failse-negative result by realtime PCR method in field diagnosis of ASFV. The needs consideration when selecting the appropriate molecular diagnostic methods is based on the current databases of ASFV sequences,  particularly for epidemiological surveillance of ASF. Keywords: African swine fever, PCR, Pigs, Real-time PCR, Vietna

Brza serotipizacija virusa afričke kuge svinja zasnovana na analizi kratkih fragmenata EP402R gena koji kodira CD2-like protein

The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.Prvi potvrđen slučaj afričke kuge svinja (ASF) u Vijetnamu bio je zvanično prijavljen u februaru 2019. godine. Do danas, ASF virus (ASFV) je detektovan u 63 provincije Vijetnama. Radi boljeg razumevanja potencijalne uloge EP402R gena u grupisanju ASFV serotipova, analizirano je trideset ASFV sekvenci EP402R gena (pristupni brojevi: MN711757-86) poreklom iz Centralne severne obale Vijetnama i 68 referentnih uzoraka serotip grupa koji su dobro poznati iz prethodnih studija. Interesantan je bio nalaz da je kratak fragment od 90 nukleotida bio tipičan za 8 seroloških grupa ASF virusa. Dizajniran je set prajmera sa ciljem amplifi kacije kratkih fragmenata (90 nukleotida), korišćenjem Primer3 programa, a za uspostavljanje jednostavne metode serotipiziranja ASFV. Rezultati ukazuju da je fi logenetska analiza kratkih fragmenata (90 nukleotida) EP402R gena je veoma specifična i predstavlja korisnu metodu serotipizacije ASFV u poređenju sa prethodno opisanim metodama koje su koristile duge fragmente (816 nukleotida) ovog gena kao i dobro poznate referentne serotipove dobijene metodom inhibicije hemadsorbcije

Enabling D2D Transmission Mode with Energy Harvesting and Information Transfer in Heterogeneous Networks

The concept of energy harvesting-assisted relay has been introduced to support the relaying transmission using Device-to-Device (D2D) communications for enhancing communication reliability. Motivated by the recent advance in Heterogeneous Network (HetNet) using relaying techniques, we consider the D2D communication provided by Energy Harvesting (EH) assisted relay where signal is forwarded from a Base Station (BS) to the conventional cellular user (non-D2D user) and D2D user. We first derive the outage probability by taking into account the SNR and power allocation parameters, and propose the transmission mode for D2D link as well as non-D2D link. After deriving the outage probability of the D2D-HetNet, we explore the effects of the network parameters on the outage probability and throughput

Extract and fraction of cashew nut testa ameliorate the hyperglycemic mice induced by Streptozotocin and high-fat diet

Drug strategy is a standard method for treating type 2 diabetes mellitus (T2D), a non-communicable disease with increasing prevalence, which may cause side effects. Therefore, natural compounds with limited adverse effects have come back into vogue for treating T2D. This study aims to evaluate the effects on rehabilitating hyperglycemic mice of cashew nut testa (husk) extract and fraction known as potential bio-substances for improvement in T2D. First, the hyperglycemic mice were induced with a high-fat diet (HFD) for 4 weeks and then were injected with streptozotocin (STZ, dozen for injection was 40 mg/kg/week) for 2 weeks. Next, the confirmed hyperglycemic mice were treated with pioglitazone (HG+PG group), total extract (HG+TE group), and saponin-rich fraction (HG+SRF group) for 3 weeks. Then, the evaluation was based on body mass; blood glucose (BG) level; BG tolerance, lipid profile, pancreatic histology and the expression IRS-1 in the pancreas. The results showed that body mass and BG level significantly increased in hyperglycemic mice. After substance treatment, there was no change in body mass in TE and SRF groups. However, BG level of HG+TE group mice significantly decreased compared to hyperglycemic mice and only BG tolerance of HG+SRF group was improved. Besides, HG+TE and HG+SRF groups modulated the triglyceride, HDL and LDL close to those expressed in normal mice. In addition, histological images of the pancreas revealed the restoration in both HG+TE and HG+SRF groups. Simultaneously, the IRS-1 expression in HG+TE group pancreas was restored to its expression in normal mice. These results demonstrate that the TE and SRF of cashew nut testa could ameliorate BG, lipid profile and pancreatic IRS-1 expression and restore the damaged pancreas and islets in hyperglycemic mice