42 research outputs found
Heat flux-based strategies for the thermal monitoring of sub-fumarolic areas: Examples from Vulcano and La Soufrière de Guadeloupe
Although it is relatively easy to set-up, the monitoring of soil temperature in sub-fumarolic areas is quite rarely
used to monitor the evolution of hydrothermal systems. Indeed, measurements are highly sensitive to environmental
conditions, in particular daily and seasonal variations of atmospheric temperatures and rainfalls, which
can be only partially filtered by the established statistical analysis. In this paper, we develop two innovative processingmethods,
both based on the computation of the heat flux in the soil. The upward heat flux method (UHF),
designed for dry environments, consists in computing both the conductive and convective components of the
heat flux between two thermocouples placed vertically. In the cases of wet environments, the excess of total
heat method (ETH) allows the integration of rain gauges data in order to correct the heat balance fromthe superficial
cooling effect of the precipitations. The performances of both processing techniques are faced to established
methods (temperature gradient and coefficient of determination) on soil temperature time series from two test
volcanoes. At La Fossa di Vulcano (Italy), the UHF method undoubtedly detects three thermal crises between
2009 and 2012, enabling to quantify not only the intensity but also the precise timing of the heat flux increase
with respect to corresponding geochemical and seismic crises. At La Soufrière de Guadeloupe (French Lesser Antilles),
despite large rainfalls dramatically influencing the thermal behavior of the soil, a constant geothermal heat
flux is retrieved by the ETH method, confirming the absence of fumarolic crisis during the observation period
(February–August 2010). Being quantitative, robust, and usable in almost any context of sub-fumarolic zones,
our two heat flux-based methods increase the potential of soil temperature for the monitoring, but also the general
interpretation of fumarolic crises together with geochemical and seismological observations. A spreadsheet
allowing direct computation of UHF and ETH is provided as supplemental material.Published122-1342V. Struttura e sistema di alimentazione dei vulcaniJCR Journa
Development and application of Single Primer Enrichment Technology (SPET) SNP assay for population genomics analysis and candidate gene discovery in lettuce
Single primer enrichment technology (SPET) is a novel high-throughput genotyping method based on short-read sequencing of specific genomic regions harboring polymorphisms. SPET provides an efficient and reproducible method for genotyping target loci, overcoming the limits associated with other reduced representation library sequencing methods that are based on a random sampling of genomic loci. The possibility to sequence regions surrounding a target SNP allows the discovery of thousands of closely linked, novel SNPs. In this work, we report the design and application of the first SPET panel in lettuce, consisting of 41,547 probes spanning the whole genome and designed to target both coding (~96%) and intergenic (~4%) regions. A total of 81,531 SNPs were surveyed in 160 lettuce accessions originating from a total of 10 countries in Europe, America, and Asia and representing 10 horticultural types. Model ancestry population structure clearly separated the cultivated accessions (Lactuca sativa) from accessions of its presumed wild progenitor (L. serriola), revealing a total of six genetic subgroups that reflected a differentiation based on cultivar typology. Phylogenetic relationships and principal component analysis revealed a clustering of butterhead types and a general differentiation between germplasm originating from Western and Eastern Europe. To determine the potentiality of SPET for gene discovery, we performed genome-wide association analysis for main agricultural traits in L. sativa using six models (GLM naive, MLM, MLMM, CMLM, FarmCPU, and BLINK) to compare their strength and power for association detection. Robust associations were detected for seed color on chromosome 7 at 50 Mbp. Colocalization of association signals was found for outer leaf color and leaf anthocyanin content on chromosome 9 at 152 Mbp and on chromosome 5 at 86 Mbp. The association for bolting time was detected with the GLM, BLINK, and FarmCPU models on chromosome 7 at 164 Mbp. Associations were detected in chromosomal regions previously reported to harbor candidate genes for these traits, thus confirming the effectiveness of SPET for GWAS. Our findings illustrated the strength of SPET for discovering thousands of variable sites toward the dissection of the genomic diversity of germplasm collections, thus allowing a better characterization of lettuce collections
Comparative studies of the water relations and the hydraulic characteristics in Fraxinus excelsior, Acer pseudoplatanus and A. opalus trees under soil water contrasted conditions
Plant water relationships and hydraulic characteristics were measured for two species of the genus
Acer that co-occur with Fraxinus excelsior, but differ in their habitat preference with respect to
soil moisture: Acer pseudoplatanus is restricted to wet habitats, whereas Acer opalus occurs on
drier sites. The data obtained showed significantly lower hydraulic conductance and lower vulnerability to
embolism in the drought-tolerant species, Acer opalus, than in the water prefering species Acer
pseudoplatanus. Similar differences in hydraulic conductance and xylem vulnerability to embolism were also
found under dry acclimated conditions for Fraxinus excelsior trees, indicating that the hydraulic
differences observed might be attributable to the contrasting soil water conditions of the sites. The
possible physiological and ecological significance of such differences are discussed, in relation to
habitat preference and the distribution of each species.Étude comparée des relations hydriques et des caractéristiques hydrauliques chez Fraxinus excelsior,
Acer pseudoplatanus et Acer opalus dans différents milieux secs et humides. Ce travail concerne l'étude
des relations hydriques et la détermination des caractéristiques hydrauliques chez deux espèces du genre
Acer, présentes fréquemment dans les espaces naturels en compagnie de Fraxinus excelsior, mais
différant dans leur mode de distribution en fonction de la disponilité de l'eau du sol : Acer
pseudoplatanus se rencontre sur des sols bien alimentés en eau, Acer opalus a une préférence marquée
pour les milieux secs. Les résultats obtenus montrent, chez Acer opalus, l'espèce tolérante à la
sécheresse, que la conductance hydraulique et la vulnérabilité à la cavitation sont moins fortes que chez
Acer pseudoplatanus, l'espèce des zones humides. Des modifications identiques de la conductance
hydraulique et de la vulnérabilité à la cavitation s'observent également chez Fraxinus excelsior pour
l'espèce acclimatée aux milieux secs, ce qui semble indiquer que ces changements des caractéristiques
hydrauliques pourraient être associés aux conditions hydriques des milieux. Ces résultats sont analysés au
plan physiologique et écologique en relation avec le mode de distribution de ces espèces dans leur
environnement respectif
Comparative studies of the water relations and the hydraulic characteristics in Fraxinus excelsior, Acer pseudoplatanus
Influence of Subseasonal Variability on the Diurnal Cycle of Precipitation on a Mountainous Island: The Case of New Caledonia
International audienceThe relationship between the large-scale intraseasonal variability, synoptic wind regimes, and the local daily variability of precipitation over the main island of New Caledonia (southwest tropical Pacific) is investigated with a focus on the austral summer wet season (November-April). The average diurnal cycle of precipitation over the island is characterized by a sharp afternoon maximum around 1600 local time, with significant differences between the windward east coast, the leeward west coast, and the mountain range. The afternoon peak is related to the afternoon sea-breeze circulation and to the diurnal cycle of convection over land. In general, its magnitude follows the same evolution as the daily mean. In agreement with past studies, a clear modulation of the Madden-Julian oscillation (MJO) on both the diurnal cycle of precipitation and the probability of occurrence of four robust wind regimes can be identified in the New Caledonia region during the wet season. From the evidence that there is a qualitative correspondence between the effects of both the MJO phases and the wind regimes on features in the diurnal cycle of precipitation, a simple model is proposed to inspect the MJO forcing mediated by wind regimes on the diurnal variability of rain. The complete decomposition of the MJO impact shows that the modulation of diurnal cycle by the MJO relies on complex interactions between the MJO and synoptic winds that involve both large-scale MJO convective anomalies and MJO-induced modification of wind patterns
Continuous photoproduction of hydrocarbon drop-in fuel by microbial cell factories
International audienceUse of microbes to produce liquid transportation fuels is not yet economically viable. A key point to reduce production costs is the design a cell factory that combines the continuous production of drop-in fuel molecules with the ability to recover products from the cell culture at low cost. Medium-chain hydrocarbons seem ideal targets because they can be produced from abundant fatty acids and, due to their volatility, can be easily collected in gas phase. However, pathways used to produce hydrocarbons from fatty acids require two steps, low efficient enzymes and/or complex electron donors. Recently, a new hydrocarbon-forming route involving a single enzyme called fatty acid photodecarboxylase (FAP) was discovered in microalgae. Here, we show that in illuminated E. coli cultures coexpression of FAP and a medium-chain fatty acid thioesterase results in continuous release of volatile hydrocarbons. Maximum hydrocarbon productivity was reached under low/medium light while higher irradiance resulted in decreased amounts of fAp. it was also found that the production rate of hydrocarbons was constant for at least 5 days and that 30% of total hydrocarbons could be collected in the gas phase of the culture. this work thus demonstrates that the photochemistry of the fAp can be harnessed to design a simple cell factory that continuously produces hydrocarbons easy to recover and in pure form
Influence of rainfalls on heat and steam fluxes of fumarolic zones: Six months records along the Ty fault (Soufrière of Guadeloupe, Lesser Antilles)
International audienceFumarolic zones are permeable areas where both steam and heat are expelled to the atmosphere. Surface fluxes and flows, which are representative of the intensity of the hydrothermal circulation in depth, can be monitored by thermometers, thermal infrared cameras, spectrometers, or condensers. However, the superficial activity of fumarolic zones can be modified by the meteorological conditions, in particular the rainfalls, which might result in erroneous estimations. From this perspective, we developed a set of physical equations to quantify the effects of rainfalls on the thermal behavior of fumarolic zones. Results were faced to continuous measurements achieved at the Ty fault fumarolic zone (La Soufrière volcano, Guadeloupe, Lesser Antilles) during six months in 2010, using six vertical series of thermometers measuring the heat transfer in the ground and one condenser measuring the rising steam flux. Results demonstrate that in the absence of rainfalls, heat and steam flux reach an equilibrium that is representa tive of the geothermal flux in depth. Conversely, after the rainfalls, the cooling of the ground provokes a deepening of the condensation level. The related soil temperature drop can be estimated by computing the heat required to warm the infiltrated water up to boiling temperature while the recovery rate is directly linked to the geothermal flux. Our observations allow defining in which conditions flux are at steady state, but also to build a first order numer ical model allowing estimating both the physical parameters of the ground (thermal conductivity, precipitation efficiency coefficient and surface flux constant) and the long term thermal behavior of the hydrothermal system. In particular, our results predict that the hydrothermal activity must vanish on the zones where the geothermal flux drops under a certain threshold (60 W/m 2 at La Soufrière). The existence of this limit may have strong implications for the precipitation rate of minerals and the possible reactivation of the fumarolic zones during volcanic crises
Autocatalytic effect boosts the production of medium-chain hydrocarbons by fatty acid photodecarboxylase
International audienceOngoing climate change is driving the search for renewable and carbon-neutral alternatives to fossil fuels. Photocatalytic conversion of fatty acids to hydrocarbons by fatty acid photodecarboxylase (FAP) represents a promising route to green fuels. However, the alleged low activity of FAP on C2 to C12 fatty acids seemed to preclude the use for synthesis of gasoline-range hydrocarbons. Here, we reveal that Chlorella variabilis FAP ( Cv FAP) can convert n -octanoic acid in vitro four times faster than n -hexadecanoic acid, its best substrate reported to date. In vivo, this translates into a Cv FAP-based production rate over 10-fold higher for n -heptane than for n -pentadecane. Time-resolved spectroscopy and molecular modeling demonstrate that Cv FAP’s high catalytic activity on n -octanoic acid is, in part, due to an autocatalytic effect of its n -heptane product, which fills the rest of the binding pocket. These results represent an important step toward a bio-based and light-driven production of gasoline-like hydrocarbons
Microalgae synthesize hydrocarbons from long-chain fatty acids via a light-dependent pathway
International audienc