Acute Ingestion of Dark Chocolate Fails to Affect Running Economy in Recreational Female Runners

Topics in Exercise Science and Kinesiology Volume 5: Issue 1, Article 1, 2024. Ingestion of dark chocolate (DC), a dietary source high in flavanols, may increase nitric oxide bioavailability. Elevating blood nitric oxide concentrations may augment metabolic efficiency by reducing the amount of oxygen or energy needed to perform a given task. Utilizing a crossover design, the purpose of this study was to investigate the effect of acute ingestion of DC on running economy (RE). Nineteen recreationally trained females (age: 20±1 years) volunteered for this investigation, with 16 completing all procedures (n = 16). Two-hours before RE assessment, participants consumed either 42.5 g of DC or an isocaloric amount of white chocolate (WC) (37.2 g) with a 34 mg caffeine pill. Participants ran on the treadmill at 2.68 m/s for 10-minutes to assess RE. However, only the last 5-minutes of the test were used for oxygen utilization (VO2), energy expenditure (EE), and respiratory exchange ratio (RER) determination via indirect calorimetry. Identical testing procedures were utilized for DC and WC treatments with a seven-day washout period separating trials. A repeated measure paired t-test was used to determine differences between dependent variables with statistical significance set at p \u3c 0.05. There were no significant mean differences (ps \u3e 0.05) between trials for VO2, EE, or RER. In conclusion, supplementation of DC 2-hours prior to steady state running had no effect on RE or fuel utilization compared to an isocaloric serving of WC in recreational female runners

Prostitution or partnership? Wifestyles in Tanzanian artisanal gold-mining settlements

Tanzania, along with several other African countries, is experiencing a national mining boom, which has prompted hundreds of thousands of men and women to migrate to mineral-rich locations. At these sites, relationships between the sexes defy the sexual norms of the surrounding countryside to embrace new relational amalgams of polygamy, monogamy and promiscuity. This article challenges the assumption that female prostitution is widespread. Using interview data with women migrants, we delineate six ‘wifestyles’, namely sexual-cum-conjugal relationships between men and women that vary in their degree of sexual and material commitment. In contrast to bridewealth payments, which involved elders formalising marriages through negotiations over reproductive access to women, sexual negotiations and relations in mining settlements involve men and women making liaisons and co-habitation arrangements directly between each other without third-party intervention. Economic interdependence may evolve thereafter with the possibility of women, as well as men, offering material support to their sex partners

Modelling leaf photosynthetic and transpiration temperature-dependent responses in Vitis vinifera cv. Semillon grapevines growing in hot, irrigated vineyard conditions

Grapevines growing in Australia suffer from high temperatures which have major effects on photosynthesis and transpiration. To learn more, gas exchange was measured over several seasons and then modelled across temperatures from 20 to 45°C and validated with independent data

Toy nanoindentation model and incipient plasticity

A toy model of two dimensional nanoindentation in finite crystals is proposed. The crystal is described by periodized discrete elasticity whereas the indenter is a rigid strain field of triangular shape representing a hard knife-like indenter. Analysis of the model shows that there are a number of discontinuities in the load vs penetration depth plot which correspond to the creation of dislocation loops. The stress vs depth bifurcation diagram of the model reveals multistable stationary solutions that appear as the dislocation-free branch of solutions develops turning points for increasing stress. Dynamical simulations show that an increment of the applied load leads to nucleation of dislocation loops below the nanoindenter tip. Such dislocations travel inside the bulk of the crystal and accommodate at a certain depth in the sample. In agreement with experiments, hysteresis is observed if the stress is decreased after the first dislocation loop is created. Critical stress values for loop creation and their final location at equilibrium are calculated.Comment: 22 pages, 5 figures, to appear in Chaos, Solitons and Fractal

Inquiries into the Nature of Free Energy and Entropy in Respect to Biochemical Thermodynamics

Free energy and entropy are examined in detail from the standpoint of classical thermodynamics. The approach is logically based on the fact that thermodynamic work is mediated by thermal energy through the tendency for nonthermal energy to convert spontaneously into thermal energy and for thermal energy to distribute spontaneously and uniformly within the accessible space. The fact that free energy is a Second-Law, expendable energy that makes it possible for thermodynamic work to be done at finite rates is emphasized. Entropy, as originally defined, is pointed out to be the capacity factor for thermal energy that is hidden with respect to temperature; it serves to evaluate the practical quality of thermal energy and to account for changes in the amounts of latent thermal energies in systems maintained at constant temperature. A major objective was to clarify the means by which free energy is transferred and conserved in sequences of biological reactions coupled by freely diffusible intermediates. In achieving this objective it was found necessary to distinguish between a 'characteristic free energy' possessed by all First-Law energies in amounts equivalent to the amounts of the energies themselves and a 'free energy of concentration' that is intrinsically mechanical and relatively elusive in that it can appear to be free of First-Law energy. The findings in this regard serve to clarify the fact that the transfer of chemical potential energy from one repository to another along sequences of biological reactions of the above sort occurs through transfer of the First-Law energy as thermal energy and transfer of the Second-Law energy as free energy of concentration.Comment: 18-page PDF; major correction in APPENDIX; minor corrections elsewher

The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm

Sperm capacitation is required for fertilization. At the molecular level, this process is associated with fast activation of protein kinase A. Downstream of this event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity of the tyrosine kinase(s) mediating this process has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase. However, crucially, loss-of-function experiments have not been reported. Here, we used both pharmacological inhibitors and genetically modified mice models to investigate the identity of the tyrosine kinase(s) mediating the increase in tyrosine phosphorylation in mouse sperm. Similar to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphorylation. Yet, sperm from Pyk2(-/-) mice displayed a normal increase in tyrosine phosphorylation, implying that PYK2 is not responsible for this phosphorylation process. Here, we show that PF431396 can also inhibit FER, a tyrosine kinase known to be present in sperm. Sperm from mice targeted with a kinase-inactivating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphorylation. Although these mice are fertile, their sperm displayed a reduced ability to fertilize metaphase II-arrested eggs in vitro.Fil: Alvau, Antonio. University of Massachussets; Estados UnidosFil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gervasi, Maria Gracia. University of Massachussets; Estados UnidosFil: Navarrete, Felipe A.. University of Massachussets; Estados UnidosFil: Xu, Xinran. State University of Colorado - Fort Collins; Estados UnidosFil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: De la Vega Beltran, José Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Greer, Peter. Queens University; CanadáFil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Krapf, Diego. State University of Colorado - Fort Collins; Estados UnidosFil: Salicioni, Ana María. University of Massachussets; Estados UnidosFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Visconti, Pablo E.. University of Massachussets; Estados Unido

Quantifying Exceptionally Large Populations of \u3ci\u3eAcropora\u3c/i\u3e spp. Corals Off Belize Using Sub-Meter Satellite Imagery Classification

Caribbean coral reefs have experienced dramatic declines in live coral cover in recent decades. Primary branching framework Caribbean corals, Acropora cervicornis (Lamarck, 1816) and Acropora palmata (Lamarck, 1816), have suffered the greatest collapse. Coral Gardens, Belize, is one of few remaining, and perhaps the largest, refugia for abundant, healthy, but undocumented populations of both Acropora species in the Caribbean Sea. In the present study, GeoEye-1 multispectral satellite imagery of a 25 km2 reefal area near Ambergris Caye, Belize, was analyzed to identify live Acropora spp. cover. We used a supervised classification to predict occurrence of areas with live Acropora spp. and to separate them from other benthic cover types, such as sandy bottom, seagrass, and mixed massive coral species. We tested classification accuracy in the field, and new Acropora spp. patches were mapped using differential GPS. Of 11 predicted new areas of Acropora spp., eight were composed of healthy Acropora spp. An unsupervised classification of a red (Band 3):blue (Band 1) ratio calculation of the image successfully separated Acropora corals from other benthic cover, with an overall accuracy of 90%. Our study identified 7.58 ha of reef dominated by Acropora spp. at Coral Gardens, which is one of the largest populations in the Caribbean Sea. We suggest that Coral Gardens may be an important site for the study of modern Acropora spp. resilience. Our technique can be used as an efficient tool for genera-specific identification, monitoring, and conservation of populations of endangered Acropora spp