145 research outputs found
Communication
The geometry of reaction compartments can affect the local outcome of interface-restricted reactions. Giant unilamellar vesicles (GUVs) are commonly used to generate cell-sized, membrane-bound reaction compartments, which are, however, always spherical. Herein, we report the development of a microfluidic chip to trap and reversibly deform GUVs into cigar-like shapes. When trapping and elongating GUVs that contain the primary protein of the bacterial Z ring, FtsZ, we find that membrane-bound FtsZ filaments align preferentially with the short GUV axis. When GUVs are released from this confinement and membrane tension is relaxed, FtsZ reorganizes reversibly from filaments into dynamic rings that stabilize membrane protrusions; a process that allows reversible GUV deformation. We conclude that microfluidic traps are useful for manipulating both geometry and tension of GUVs, and for investigating how both affect the outcome of spatially-sensitive reactions inside them, such as that of protein self-organization.We acknowledge the MPIB Biochemistry Core Facility for assistance in protein purification
Acceptability with general orderings
We present a new approach to termination analysis of logic programs. The
essence of the approach is that we make use of general orderings (instead of
level mappings), like it is done in transformational approaches to logic
program termination analysis, but we apply these orderings directly to the
logic program and not to the term-rewrite system obtained through some
transformation. We define some variants of acceptability, based on general
orderings, and show how they are equivalent to LD-termination. We develop a
demand driven, constraint-based approach to verify these
acceptability-variants.
The advantage of the approach over standard acceptability is that in some
cases, where complex level mappings are needed, fairly simple orderings may be
easily generated. The advantage over transformational approaches is that it
avoids the transformation step all together.
{\bf Keywords:} termination analysis, acceptability, orderings.Comment: To appear in "Computational Logic: From Logic Programming into the
Future
New results on rewrite-based satisfiability procedures
Program analysis and verification require decision procedures to reason on
theories of data structures. Many problems can be reduced to the satisfiability
of sets of ground literals in theory T. If a sound and complete inference
system for first-order logic is guaranteed to terminate on T-satisfiability
problems, any theorem-proving strategy with that system and a fair search plan
is a T-satisfiability procedure. We prove termination of a rewrite-based
first-order engine on the theories of records, integer offsets, integer offsets
modulo and lists. We give a modularity theorem stating sufficient conditions
for termination on a combinations of theories, given termination on each. The
above theories, as well as others, satisfy these conditions. We introduce
several sets of benchmarks on these theories and their combinations, including
both parametric synthetic benchmarks to test scalability, and real-world
problems to test performances on huge sets of literals. We compare the
rewrite-based theorem prover E with the validity checkers CVC and CVC Lite.
Contrary to the folklore that a general-purpose prover cannot compete with
reasoners with built-in theories, the experiments are overall favorable to the
theorem prover, showing that not only the rewriting approach is elegant and
conceptually simple, but has important practical implications.Comment: To appear in the ACM Transactions on Computational Logic, 49 page
Quantifier-Free Interpolation of a Theory of Arrays
The use of interpolants in model checking is becoming an enabling technology
to allow fast and robust verification of hardware and software. The application
of encodings based on the theory of arrays, however, is limited by the
impossibility of deriving quantifier- free interpolants in general. In this
paper, we show that it is possible to obtain quantifier-free interpolants for a
Skolemized version of the extensional theory of arrays. We prove this in two
ways: (1) non-constructively, by using the model theoretic notion of
amalgamation, which is known to be equivalent to admit quantifier-free
interpolation for universal theories; and (2) constructively, by designing an
interpolating procedure, based on solving equations between array updates.
(Interestingly, rewriting techniques are used in the key steps of the solver
and its proof of correctness.) To the best of our knowledge, this is the first
successful attempt of computing quantifier- free interpolants for a variant of
the theory of arrays with extensionality
Управління виробничими запасами на підприємстві (на матеріалах ПрАТ «Детвілер Ущільнюючі Технології України»)
. The second-order matching problem is the problem of determining, for a finite set {#t i , s i # | i # I} of pairs of a second-order term t i and a first-order closed term s i , called a matching expression, whether or not there exists a substitution # such that t i # = s i for each i # I . It is well-known that the second-order matching problem is NP-complete. In this paper, we introduce the following restrictions of a matching expression: k-ary, k-fv , predicate, ground , and function-free. Then, we show that the second-order matching problem is NP-complete for a unary predicate, a unary ground, a ternary function-free predicate, a binary function-free ground, and an 1-fv predicate matching expressions, while it is solvable in polynomial time for a binary function-free predicate, a unary function-free, a k-fv function-free (k # 0), and a ground predicate matching expressions. 1 Introduction The unification problem is the problem of determining whether or not any two ter..
Lynx: A Programmatic SAT Solver for the RNA-folding Problem
15th International Conference, Trento, Italy, June 17-20, 2012. ProceedingsThis paper introduces Lynx, an incremental programmatic SAT solver that allows non-expert users to introduce domain-specific code into modern conflict-driven clause-learning (CDCL) SAT solvers, thus enabling users to guide the behavior of the solver.
The key idea of Lynx is a callback interface that enables non-expert users to specialize the SAT solver to a class of Boolean instances. The user writes specialized code for a class of Boolean formulas, which is periodically called by Lynx’s search routine in its inner loop through the callback interface. The user-provided code is allowed to examine partial solutions generated by the solver during its search, and to respond by adding CNF clauses back to the solver dynamically and incrementally. Thus, the user-provided code can specialize and influence the solver’s search in a highly targeted fashion. While the power of incremental SAT solvers has been amply demonstrated in the SAT literature and in the context of DPLL(T), it has not been previously made available as a programmatic API that is easy to use for non-expert users. Lynx’s callback interface is a simple yet very effective strategy that addresses this need.
We demonstrate the benefits of Lynx through a case-study from computational biology, namely, the RNA secondary structure prediction problem. The constraints that make up this problem fall into two categories: structural constraints, which describe properties of the biological structure of the solution, and energetic constraints, which encode quantitative requirements that the solution must satisfy. We show that by introducing structural constraints on-demand through user provided code we can achieve, in comparison with standard SAT approaches, upto 30x reduction in memory usage and upto 100x reduction in time
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