Genistein-induced mir-23b expression inhibits the growth of breast cancer cells

Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells. Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR. Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was up-regulated for MCF-7 breast cancer cells after genistein treatment. Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein. © 2015, Termedia Publishing House Ltd. All rights reserved

Evaluation of candidate gene effects and environmental factors on reproductive performance of Holstein cows

This study investigated the impact of nine polymorphisms located in the CSN2, CSN3, CSN1S1, CSN1S2, OLR1, LALBA, STAT1, DGAT1 and LGB genes, and environmental factors including calving year, season and parity on reproductive traits. The analysis was conducted on 165 Holstein-Friesian cows. Genotypes were identified using PCR-RFLP. The data of reproductive traits for four lactations were evaluated. Statistical analysis was carried out using least squares of the GLM procedures. Results indicated that CSN2 had significant effects on days before first insemination and first insemination to pregnancy interval. The SNP at the CSN3 was significantly associated with gestation length. A novel effect of OLR1-C223A on age at first calving was observed in the present study. Moreover, DGAT1 and LGB markers were significantly associated with calving interval and days before first insemination, respectively. In addition, significant environmental effects were as follows: calving year with days before first oestrus, days open, and first insemination to pregnancy interval; season with days open, first insemination to pregnancy interval, and calving interval; parity with days before first oestrus and days open. The present results and novel associations may therefore be useful and indicative for future studies on a genetic basis of cattle reproduction traits.Keywords: cattle, environmental effects, genetic marker, Holstein-Friesian, reproduction parameter

The effect of anatomic differences on the relationship between renal artery and diaphragmatic crus

Background: The aim of this study is to investigate the effect of anatomic differences on the relationship between renal artery and diaphragmatic crus via the touch of two structures. Materials and methods: The study included dynamic computed tomography (CT) scans of 308 patients performed mainly for characterisation of liver and renal masses. Anatomic differences including the thickness of the diaphragmatic crus, the localisation of renal artery ostium at the wall of aorta, the level of renal artery origin with respect to superior mesenteric artery were evaluated. Statistical relationships between renal artery-diaphragmatic crus contact and the anatomic differences were assessed. Results: Thickness of the diaphragmatic crus at the level of renal artery origin exhibited a statistically significant relationship to renal artery-diaphragmatic crus contact at the left (p < 0.001) and right side (p < 0.001). There was a statistically significant relationship between high renal artery origin and renal artery- -diaphragmatic crus contact at the left (p < 0.001) and right side (p = 0.01). The localisation of renal artery ostium at the wall of aorta (right side, p = 0.436, left side, p = 0.681) did not demonstrate a relationship to renal artery-diaphragmatic crus contact. Conclusions: Thickness of the diaphragmatic crus and high renal artery origin with respect to superior mesenteric artery are crucial anatomic differences determining the relationship of renal artery and diaphragmatic crus. (Folia Morphol 2018; 77, 1: 22–28)

Polypyrrole grafts with poly[(methyl methacrylate)-CO-(2-(N-pyrrolyl)ethyl methacrylate)]

Cataloged from PDF version of article.Conducting polymer grafts of pyrrole and poly[(methyl methacrylate)-co-(2- (N-pyrrolyl) ethyl methacrylate)] containing 0.7% PEMA units were prepared by potentiostatic anodic polymerization of pyrrole in different electrolytic media. Grafting between copolymer and pyrrole was achieved in media where tetrabutylammonium fluoroborate and sodium perchlorate were used as the supporting electrolytes. Characterizations were made by using IT-IR, DSC, TGA, SEM, CV, and elemental analysis. The conductivities of the resultant polymers seemed to be in the order of pure polypyrrole prepared under the same conditions. Copyright o 1997 Elsevier Scienc

Providing Reusability and Learning Support in the Simulation Model Development Environment

The Premodels Manager, one of the tools of the Simulation Model Development Environment (SMDE), is required to enable a user to locate and reuse components of successfully completed simulation studies and learn from past experience. This paper presents the SMDE Premodels Manager and describes how it provides reusability and learning support. Objectives are set forth and a design is established and implemented on a Sun workstation. The SMDE Premodels Manager consists of four working windows, three access windows, and three support windows. It uses the SMDE Premodels Database which is a highly modifiable repository of documentation on successfully completed simulation studies. It is evaluated with respect to the design objectives and is shown to provide effective reusability and learning support within the SMDE

Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗBovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle

Bscl2 Deficiency Does Not Directly Impair the Innate Immune Response in a Murine Model of Generalized Lipodystrophy

Funding: Work was supported by Diabetes UK (JJR;18/0005884, MD;17/0005621) the Medical Research Council (JJR; MR/L002620/1, MC/PC/15077), the British Heart Foundation (MD; PG/14/43/30889), The Agency for Science, Technology and Research, Singapore (A*STAR) (WH), The Wellcome Trust (ISSF Funding to GDM) and the European Union’s Horizon 2020 ERC consolidator award (MB:2016-726152-TYPHI).Peer reviewedPublisher PD

Establishing Software Development Process Control: Technical Objectives, Operational Requirements, and the Foundational Framework

This paper proposes a foundational framework for establishing control over the software development process. Critical objectives stressed include (a) the complementary integration of maintenance and development activities, (b) the identification and definition of a (semi-) automated data collection and analysis process which employs quality indicators that are definitively linked to the existence of process and product attributes, and (c) the formulation and use of control methods that are designed to work within the defined automated process and to provide decision support capabilities. The significance and necessity of these objectives are established through an examination of the Abstraction Refinement Model, the Objectives/Principles/Attributes Framework and the Software Quality Indicator concept