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research
Genistein-induced mir-23b expression inhibits the growth of breast cancer cells
Authors
C.B. Avci
D. Aygunes
+5 more
T. Balci
H.O. Caglar
Yavuz Dodurga
C. Gunduz
S.Y. Susluer
Publication date
1 January 2015
Publisher
'Termedia Sp. z.o.o.'
Doi
Cite
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on
PubMed
Abstract
Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells. Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR. Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was up-regulated for MCF-7 breast cancer cells after genistein treatment. Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein. © 2015, Termedia Publishing House Ltd. All rights reserved
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Pamukkale Üniversitesi Açık Erişim Arşivi
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oai:acikerisim.pau.edu.tr:1149...
Last time updated on 05/09/2019
Ege University Institutional Repository
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oai:gcris.ege.edu.tr:11454/493...
Last time updated on 23/11/2023
Pamukkale Üniversitesi Açık Erişim Arşivi
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:acikerisim.pau.edu.tr:1149...
Last time updated on 05/09/2019