Social and Economic Impact of Solar Electricity at Schuchuli Village

Schuchuli, a small remote village on the Papago Indian Reservation in southwest Arizona, is 27 kilometers (17 miles) from the nearest available utility power. Its lack of conventional power is due to the prohibitive cost of supplying a small electrical load with a long-distance distribution line. Furthermore, alternate energy sources are expensive and place a burden on the resources of the villagers. On December 16, 1978, as part of a federally funded project, a solar cell power system was put into operation at Schuchuli. The system powers the village water pump, lighting for homes and other village buildings, family refrigerators and a communal washing machine and sewing machine

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue -- III. Two new low-mass systems with rapidly evolving spots

We present the results of our spectroscopic and photometric analysis of two newly discovered low-mass detached eclipsing binaries found in the All-Sky Automated Survey (ASAS) catalogue: ASAS J093814-0104.4 and ASAS J212954-5620.1. Using the GIRAFFE instrument on the 1.9-m Radcliffe telescope at SAAO and the UCLES spectrograph on the 3.9-m Anglo-Australian Telescope, we obtained high-resolution spectra of both objects and derived their radial velocities (RVs) at various orbital phases. The RVs of both objects were measured with the TODCOR technique using synthetic template spectra as references. We also obtained V and I band photometry using the 1.0-m Elizabeth telescope at SAAO and the 0.4-m PROMPT instruments located at the CTIO. The orbital and physical parameters of the systems were derived with PHOEBE and JKTEBOP codes. We compared our results with several sets of widely-used isochrones. Our multi-epoch photometric observations demonstrate that both objects show significant out-of-eclipse modulations, which vary in time. We believe that this effect is caused by stellar spots, which evolve on time scales of tens of days. For this reason, we constructed our models on the basis of photometric observations spanning short time scales (less than a month). Our modeling indicates that (1) ASAS-09 is a main sequence active system with nearly-twin components with masses of M1 = 0.771(33) Msun, M2 = 0.768(21) Msun and radii of R1 = 0.772(12) Rsun and R2 = 0.769(13) Rsun. (2) ASAS-21 is a main sequence active binary with component masses of M1 = 0.833(17) Msun, M2 = 0.703(13) Msun and radii of R1 = 0.845(12) Rsun and R2 = 0.718(17) Rsun. Both systems confirm the characteristic of active low-mass stars, for which the observed radii are larger and the temperatures lower than predicted by evolutionary models. Other parameters agree within errors with the models of main sequence stars.Comment: 15 pages, 7 figures, 7 tables, to appear in A&

Collision Dynamics and Solvation of Water Molecules in a Liquid Methanol Film

Environmental molecular beam experiments are used to examine water interactions with liquid methanol films at temperatures from 170 K to 190 K. We find that water molecules with 0.32 eV incident kinetic energy are efficiently trapped by the liquid methanol. The scattering process is characterized by an efficient loss of energy to surface modes with a minor component of the incident beam that is inelastically scattered. Thermal desorption of water molecules has a well characterized Arrhenius form with an activation energy of 0.47{\pm}0.11 eV and pre-exponential factor of 4.6 {\times} 10^(15{\pm}3) s^(-1). We also observe a temperature dependent incorporation of incident water into the methanol layer. The implication for fundamental studies and environmental applications is that even an alcohol as simple as methanol can exhibit complex and temperature dependent surfactant behavior.Comment: 8 pages, 5 figure

Circadian and Feeding Rhythms Orchestrate the Diurnal Liver Acetylome.

Lysine acetylation is involved in various biological processes and is considered a key reversible post-translational modification in the regulation of gene expression, enzyme activity, and subcellular localization. This post-translational modification is therefore highly relevant in the context of circadian biology, but its characterization on the proteome-wide scale and its circadian clock dependence are still poorly described. Here, we provide a comprehensive and rhythmic acetylome map of the mouse liver. Rhythmic acetylated proteins showed subcellular localization-specific phases that correlated with the related metabolites in the regulated pathways. Mitochondrial proteins were over-represented among the rhythmically acetylated proteins and were highly correlated with SIRT3-dependent deacetylation. SIRT3 activity being nicotinamide adenine dinucleotide (NAD) <sup>+</sup> level-dependent, we show that NAD <sup>+</sup> is orchestrated by both feeding rhythms and the circadian clock through the NAD <sup>+</sup> salvage pathway but also via the nicotinamide riboside pathway. Hence, the diurnal acetylome relies on a functional circadian clock and affects important diurnal metabolic pathways in the mouse liver

The novel CXCR4 antagonist POL5551 mobilizes hematopoietic stem and progenitor cells with greater efficiency than Plerixafor

Mobilized blood has supplanted bone marrow (BM) as the primary source of hematopoietic stem cells for autologous and allogeneic stem cell transplantation. Pharmacologically enforced egress of hematopoietic stem cells from BM, or mobilization, has been achieved by directly or indirectly targeting the CXCL12/CXCR4 axis. Shortcomings of the standard mobilizing agent, granulocyte colony-stimulating factor (G-CSF), administered alone or in combination with the only approved CXCR4 antagonist, Plerixafor, continue to fuel the quest for new mobilizing agents. Using Protein Epitope Mimetics technology, a novel peptidic CXCR4 antagonist, POL5551, was developed. In vitro data presented herein indicate high affinity to and specificity for CXCR4. POL5551 exhibited rapid mobilization kinetics and unprecedented efficiency in C57BL/6 mice, exceeding that of Plerixafor and at higher doses also of G-CSF. POL5551-mobilized stem cells demonstrated adequate transplantation properties. In contrast to G-CSF, POL5551 did not induce major morphological changes in the BM of mice. Moreover, we provide evidence of direct POL5551 binding to hematopoietic stem and progenitor cells (HSPCs) in vivo, strengthening the hypothesis that CXCR4 antagonists mediate mobilization by direct targeting of HSPCs. In summary, POL5551 is a potent mobilizing agent for HSPCs in mice with promising therapeutic potential if these data can be orroborated in humans

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue - IV. A 0.61 + 0.45 M_sun binary in a multiple system

We present the orbital and physical parameters of a newly discovered low-mass detached eclipsing binary from the All-Sky Automated Survey (ASAS) database: ASAS J011328-3821.1 A - a member of a visual binary system with the secondary component separated by about 1.4 seconds of arc. The radial velocities were calculated from the high-resolution spectra obtained with the 1.9-m Radcliffe/GIRAFFE, 3.9-m AAT/UCLES and 3.0-m Shane/HamSpec telescopes/spectrographs on the basis of the TODCOR technique and positions of H_alpha emission lines. For the analysis we used V and I band photometry obtained with the 1.0-m Elizabeth and robotic 0.41-m PROMPT telescopes, supplemented with the publicly available ASAS light curve of the system. We found that ASAS J011328-3821.1 A is composed of two late-type dwarfs having masses of M_1 = 0.612 +/- 0.030 M_sun, M_2 = 0.445 +/- 0.019 M_sun and radii of R_1 = 0.596 +/- 0.020 R_sun, R_2 = 0.445 +/- 0.024 R_sun, both show a substantial level of activity, which manifests in strong H_alpha and H_beta emission and the presence of cool spots. The influence of the third light on the eclipsing pair properties was also evaluated and the photometric properties of the component B were derived. Comparison with several popular stellar evolution models shows that the system is on its main sequence evolution stage and probably is more metal rich than the Sun. We also found several clues which suggest that the component B itself is a binary composed of two nearly identical ~0.5 M_sun stars.Comment: 12 pages, 7 figures, 7 tables, to appear in MNRA

Mobilization of human hematopoietic stem/progenitor-enriched CD34+ cells into peripheral blood during stress related to ischemic stroke.

The bone marrow-derived stem/progenitor cells were demonstrated to play an important role in a regeneration of damaged tissue. Based on these observations we asked whether the stroke-related stress triggers mobilization of stem/progenitor cells from the bone marrow into the peripheral blood, which subsequently could contribute to regeneration of damaged organs. To address this issue, the peripheral blood samples were harvested from patients with ischemic stroke during the first 24 hrs as well as after the 48 (2nd day) and 144 hrs (6th day) since the manifestation of symptoms. In these patients we evaluated the percentage of hematopoietic stem/progenitor-enriched CD34+ cells by employing flow cytometry and the number of hematopoietic progenitor cells for the granulocyto-monocytic (CFU-GM) and erythroid (BFU-E)-lineages circulating in peripheral blood. We concluded that stress related to ischemic stroke triggers the mobilization of hematopoietic stem/progenitor cells from the bone marrow into peripheral blood. These circulating stem/progenitor cells may play an important role in the process of regeneration of the ischemic tissue

Type 1 Diabetes Risk Variants Reduce Beta Cell Function

Introduction: The variants rs10517086 and rs1534422 are predictive of type 1 diabetes mellitus (T1DM) development and poor residual β cell function within the first year of diagnosis. However, the mechanism by which risk is conferred is unknown. We explored the impact of both variants on β cell function in vitro and assessed their relationship with C-peptide in people with T1DM and type 2 diabetes mellitus (T2DM). Methods: Using CRISPR/Cas9, the variants were introduced into a β cell line (BRIN-BD11) and a T cell line (Jurkat cells) from which the conditioned media was applied to otherwise healthy β cells to model the inflammatory environment associated with these variants. Results: Both variants significantly reduced glucose-stimulated insulin secretion, increased production of pro-inflammatory cytokines and reduced expression of several β cell markers and transcription factors (KCNJ11, KCNQ1, SCL2A2, GCK, NKX6.1, Pdx1 NGN3). However, HNF1A was significantly upregulated in the presence of both variants. We subsequently silenced HNF1A in variant expressing BRIN-BD11 cells using siRNA and found that gene expression profiles were normalised. Induction of each variant significantly increased expression of the lncRNAs they encode, which was normalised upon HNF1A silencing. Analysis of the DARE (Diabetes Alliance for Research in England) study revealed an association of rs10517086_A genotype with C-peptide in 153 individuals with T1DM, but not in 417 people with T2DM. Conclusions: These data suggest that rs1534422 and rs10517086 exert multiple insults on the β cell through excessive upregulation of HNF1A and induction of pro-inflammatory cytokines, and highlight their utility as prognostic markers of β cell function

Plasticity of the Muscle Stem Cell Microenvironment

Satellite cells (SCs) are adult muscle stem cells capable of repairing damaged and creating new muscle tissue throughout life. Their functionality is tightly controlled by a microenvironment composed of a wide variety of factors, such as numerous secreted molecules and different cell types, including blood vessels, oxygen, hormones, motor neurons, immune cells, cytokines, fibroblasts, growth factors, myofibers, myofiber metabolism, the extracellular matrix and tissue stiffness. This complex niche controls SC biology-quiescence, activation, proliferation, differentiation or renewal and return to quiescence. In this review, we attempt to give a brief overview of the most important players in the niche and their mutual interaction with SCs. We address the importance of the niche to SC behavior under physiological and pathological conditions, and finally survey the significance of an artificial niche both for basic and translational research purposes