RANK/RANKL/OPG pathway: genetic associations with stress fracture period prevalence in elite athletes

Context: The RANK/RANKL/OPG signalling pathway is important in the regulation of bone turnover, with single nucleotide polymorphisms (SNPs) in genes within this pathway associated with bone phenotypic adaptations. Objective: To determine whether four SNPs associated with genes in the RANK/RANKL/OPG signalling pathway were associated with stress fracture injury in elite athletes. Design, Participants, and Methods: Radiologically confirmed stress fracture history was reported in 518 elite athletes, forming the Stress Fracture Elite Athlete (SFEA) cohort. Data were analysed for the whole group, and were sub-stratified into male and cases of multiple stress fracture group. Genotypes were determined using proprietary fluorescence-based competitive allele-specific PCR assays. Results: SNPs rs3018362 (RANK) and rs1021188 (RANKL) were associated with stress fracture injury (p<0.05). 8.1% of stress fracture group and 2.8% of the non-stress fracture group were homozygote for the rare allele of rs1021188. Allele frequency, heterozygotes and homozygotes for the rare allele of rs3018362 were associated with stress fracture period prevalence (p<0.05). Analysis of the male only group showed 8.2% of rs1021188 rare allele homozygotes to have suffered a stress fracture while 2.5% of the non-stress fracture group were homozygous. In cases of multiple stress fractures, homozygotes for the rare allele of rs1021188, and individuals possessing at least one copy of the rare allele of rs4355801 (OPG) were shown to be associated with stress fracture injury (p<0.05). Conclusions: The data support an association between SNPs in the RANK/RANKL/OPG signalling pathway and the development of stress fracture injury. The association of rs3018362 (RANK) and rs1021188 (RANKL) with stress fracture injury susceptibility supports their role in the maintenance of bone health, and offers potential targets for therapeutic interventions

Hydrogeochemistry and Groundwater Quality Assessment in the High Agri Valley (Southern Italy)

The High Agri Valley (southern Italy) is one of the largest intermontane basin of the southern Apennines affected by intensive agricultural and industrial activities. The study of groundwater chemical features provides much important information useful in water resource management. In this study, hydrogeochemical investigations coupled with multivariate statistics, saturation indices, and stable isotope composition (δD and δ18O) were conducted in the High Agri Valley to determine the chemical composition of groundwater and to define the geogenic and anthropogenic influences on groundwater quality. Twenty-four sampling point ( including well and spring waters) have been examined. The isotopic data revealed that groundwater has a meteoric origin. Well waters, located on recent alluvial-lacustrine deposits in shallow porous aquifers at the valley floor, are influenced by seasonal rainfall events and show shallow circuits; conversely, spring waters from fissured and/or karstified aquifers are probably associated to deeper and longer hydrogeological circuits. The R -mode factor analysis shows that three factors explain 94% of the total variance, and F1 represents the combined effect of dolomite and silicate dissolution to explain most water chemistry. In addition, very low contents of trace elements were detected, and their distribution was principally related to natural input. Only two well waters, used for irrigation use, show critical issue for NO3- concentrations, whose values are linked to agricultural activities. Groundwater quality strongly affects the management of water resources, as well as their suitability for domestic, agricultural, and industrial uses. Overall, our results were considered fulfilling the requirements for the inorganic component of the Water Framework Directive and Italian legislation for drinking purposes. The water quality for irrigation is from "good to permissible" to "excellent to good" although salinity and relatively high content of Mg2+ can occasionally be critical

SiPM and front-end electronics development for Cherenkov light detection

The Italian Institute of Nuclear Physics (INFN) is involved in the development of a demonstrator for a SiPM-based camera for the Cherenkov Telescope Array (CTA) experiment, with a pixel size of 6$\times$6 mm$^2$. The camera houses about two thousands electronics channels and is both light and compact. In this framework, a R&D program for the development of SiPMs suitable for Cherenkov light detection (so called NUV SiPMs) is ongoing. Different photosensors have been produced at Fondazione Bruno Kessler (FBK), with different micro-cell dimensions and fill factors, in different geometrical arrangements. At the same time, INFN is developing front-end electronics based on the waveform sampling technique optimized for the new NUV SiPM. Measurements on 1$\times$1 mm$^2$, 3$\times$3 mm$^2$, and 6$\times$6 mm$^2$ NUV SiPMs coupled to the front-end electronics are presentedComment: In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

Economics of One Health: Costs and benefits of integrated West Nile virus surveillance in Emilia-Romagna

Since 2013 in Emilia-Romagna, Italy, surveillance information generated in the public health and in the animal health sectors has been shared and used to guide public health interventions to mitigate the risk of West Nile virus (WNV) transmission via blood transfusion. The objective of the current study was to identify and estimate the costs and benefits associated with this One Health surveillance approach, and to compare it to an approach that does not integrate animal health information in blood donations safety policy (uni-sectoral scenario). Costs of human, animal, and entomological surveillance, sharing of information, and triggered interventions were estimated. Benefits were quantified as the averted costs of potential human cases of WNV neuroinvasive disease associated to infected blood transfusion. In the 2009–2015 period, the One Health approach was estimated to represent a cost saving of €160,921 compared to the uni-sectoral scenario. Blood donation screening was the main cost for both scenarios. The One Health approach further allowed savings of €1.21 million in terms of avoided tests on blood units. Benefits of the One Health approach due to short-term costs of hospitalization and compensation for transfusion-associated disease potentially avoided, were estimated to range from €0 to €2.98 million according to the probability of developing WNV neuroinvasive disease after receiving an infected blood transfusion

Criticality measurements for control and accountability of fissile materials on critical assemblies

Operational critical assemblies are subject to the same standards of materials control and accountability (MC and A) as other fissile materials. Typically these assemblies are not easily dismantled and the fuel components cannot be inventoried by traditional methods of destructive or non-destructive materials assay. Because of the large mass or unusual geometry of the fuel components these parts are often categorized as difficult-to-measure (DTM) items. The use of criticality to infer fissile inventory, in conjunction with physical security and two-person control procedures, provides accurate and cost-effective MC and A for these large critical assembly components. This method is being implemented at the Los Alamos Critical Experiments Facility (LACEF) and at several national nuclear research centers in Russia

Management of sagittal craniosynostosis morphological comparison of 8 surgical techniques

The aim of this study was to carry out a retrospective multicenter study comparing the morphological outcome of 8 techniques used for the management of sagittal synostosis versus a large cohort of control patients. Computed tomography (CT) images were obtained from children CT-scanned for non-craniosynostosis related events (n=241) and SS patients at pre-operative and post-operative follow-up stages (n=101). No significant difference in morphological outcomes was observed between the techniques considered in this study. However, the majority of techniques showed a tendency for relapse. Further, the more invasive procedures at older ages seem to lead to larger intracranial volume compared to less invasive techniques at younger ages. This study can be a first step towards future multicenter studies, comparing surgical results and offering a possibility for objective benchmarking of outcomes between methods and centers

Definition of an indicator assessing the impact of a dam on the downstream river landscape

The increasing number of water withdrawals in Alpine regions represents a significant threat to aquatic ecosystems and river landscape (riverscape). To assess their sustainability, the impacts on river ecological status and landscape features need to be quantified with appropriate indicators. However, assessment of landscape attributes is a complex challenge, due to the lack of standardized methods. Moreover, few metrics quantifying the impacts of water withdrawal on downstream riverscape perception are available in the scientific literature. In this paper, a new indicator, named Landscape Protection Level (LPL), aimed at assessing the effects of water withdrawals on the river landscape, is presented. The indicator has been developed in Aosta Valley (NW Italian Alps), where the river network is heavily exploited by hundreds of withdrawals for hydropower production and irrigation, and it has been included in a multi-criteria analysis (MCA) procedure to assess the sustainability of water withdrawal licenses in relation to different flow release scenarios. The LPL indicator is based on three parameters, Constraint Factor, Release Factor, and Visual Elements Factor, quantifying the presence of landscape protection constraints, the ratio of flow released downstream of the dam to the available river discharge, and the impact on the visual perception of the bypassed stretch, respectively. Its application in four real case studies of existing hydropower plants is presented and discussed in the paper, demonstrating the indicator applicability to assess both specific release values and flow release scenarios varying over the year. Results are analyzed by highlighting the main strengths and weaknesses of the indicator and proposing some suggestions for future improvements. In particular, the reactiveness of the indicator, the representativeness of the stakeholders’ interests, the transparency of the indicator calculation procedure, and the time required for data collection and processing are discussed. Finally, future activities aimed at further improving the indicator applicability and transferability to different river contexts are proposed