Graphite Electrode Modified with a New Phenothiazine Derivative and with Carbon Nanotubes for NADH Electrocatalytic Oxidation

The electrochemical behavior of a modified electrode obtained by immobilization of single-walled carbon nanotubes onto a graphite electrode modified with a new phenothiazine derivative, bis-phenothiazin-3-yl methane (BPhM), G/BPhM-CNT, has been evaluated and compared with BPhM adsorbed on graphite electrode (G/BPhM). The G/BPhM-CNT electrode presents improved performances for NADH electrocatalytic oxidation in comparison with G/BPhM electrode, expressed by: (i) a significant increase of electrocatalytic rate constant (kobs,[NADH] 0) for NADH oxidation (856.32 L mol–1 s–1 for G/BPhM-CNT and 51.63 L mol–1 s–1 for G/BPhM, in phosphate buffer, pH 7); (ii) the obtained amperometric sensors for NADH detection present increase sensitivity (S = 6.9 mA L mol–1 for G/BPhM-CNT and S = 0.55 mA L mol–1 for G/BPhM, pH 7)

The ciliary marginal zone of the zebrafish retina: clonal and time-lapse analysis of a continuously growing tissue.

Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues (Simons and Clevers, 2011). Such an analysis, however, has not yet been achieved for continuously growing adult tissues, but is essential if we wish to understand the architecture of adult organs. The retinas of lower vertebrates grow throughout life from retinal stem cells (RSCs) and retinal progenitor cells (RPCs) at the rim of the retina, called the ciliary marginal zone (CMZ). Here, we show that RSCs reside in a niche at the extreme periphery of the CMZ and divide asymmetrically along a radial (peripheral to central) axis, leaving one daughter in the peripheral RSC niche and the other more central where it becomes an RPC. We also show that RPCs of the CMZ have clonal sizes and compositions that are statistically similar to progenitor cells of the embryonic retina and fit the same stochastic model of proliferation. These results link embryonic and postembryonic cell behaviour, and help to explain the constancy of tissue architecture that has been generated over a lifetime.This work was supported by a Wellcome Trust Senior Investigator Awards [100329/Z/12/Z to W.A.H.] and [098357/Z/12/Z to B.D.S].This is the final version of the article. It first appeared from The Company of Biologists via https://doi.org/10.1242/dev.13331

Alemtuzumab-induced halo nevus-like hypopigmentation – New insights into secondary skin autoimmunity in response to an immune cell-depleting antibody

We describe a 33‐year‐old male patient who developed hypopigmentation around his melanocytic nevi with disappearance of the nevi in August 2018. In June 2016 he had been diagnosed with highly active RRMS and treated with alemtuzumab in September 2016 for the first time. In September 2017 another cycle of alemtuzumab was administered. The patient’s family history for vitiligo was unremarkable and he had no autoimmune thyroid disease

[61] Harmonica Alley

This exhibition explores Miyakobashi Yokocho in drawings, photographs and text. It reveals previously concealed glimpses into Japan’s changing urban culture. Constructed in 1964, just in time for Tokyo’s Summer Olympics, Miyakobashi Shopping Centre transformed a formerly cluttered black market area into an orderly urban marketplace. Over several decades, courageous women converted Miyakobashi into the vibrant entertainment block it is today. Miyakobashi Yokocho is now home to sixty-one lift-sized spaces: ‘ sunakku ’ (snack bars), frequented by business men, local residents and the occasional tourist alike