Continuous laser hardening with induction pre-heating

A novel way of continuous surface hardening of steel bodies by a laser beam is modeled. This heat treatment is supplemented with pre-heating of the hardened parts by a classic inductor in order to reduce the temperature gradients and subsequent mechanical stresses in the processed material. The mathematical model of the process is solved numerically in 3D and the solution respects all important nonlinearities (a saturation curve of the hardened steel and temperature dependences of its physical properties). The methodology is illustrated with a typical example, whose results are presented and discussed

Slowly rotating voids in cosmology

We consider a spacetime consisting of an empty void separated from an almost Friedmann-Lema\^\i tre-Robertson-Walker (FLRW) dust universe by a spherically symmetric, slowly rotating shell which is comoving with the cosmic dust. We treat in a unified manner all types of the FLRW universes. The metric is expressed in terms of a constant characterizing the angular momentum of the shell, and parametrized by the comoving radius of the shell. Treating the rotation as a first order perturbation, we compute the dragging of inertial frames as well as the apparent motion of distant stars within the void. Finally, we discuss, in terms of in principle measurable quantities, 'Machian' features of the model.Comment: 21 pages, 5 figures, REVTex, accepted for publication in Class.Quant.Gravit

Environmental risk assessment of genetically modified plants - concepts and controversies

Background and purpose: In Europe, the EU Directive 2001/18/EC lays out the main provisions of environmental risk assessment (ERA) of genetically modified (GM) organisms that are interpreted very differently by different stakeholders. The purpose of this paper is to: (a) describe the current implementation of ERA of GM plants in the EU and its scientific shortcomings, (b) present an improved ERA concept through the integration of a previously developed selection procedure for identification of non-target testing organisms into the ERA framework as laid out in the EU Directive 2001/18/EC and its supplement material (Commission Decision 2002/623/EC), (c) describe the activities to be carried out in each component of the ERA and (d) propose a hierarchical testing scheme. Lastly, we illustrate the outcomes for three different crop case examples. Main features: Implementation of the current ERA concept of GM crops in the EU is based on an interpretation of the EU regulations that focuses almost exclusively on the isolated bacteria-produced novel proteins with little consideration of the whole plant. Therefore, testing procedures for the effect assessment of GM plants on non-target organisms largely follow the ecotoxicological testing strategy developed for pesticides. This presumes that any potential adverse effect of the whole GM plant and the plant-produced novel compound can be extrapolated from testing of the isolated bacteriaproduced novel compound or can be detected in agronomic field trials. This has led to persisting scientific criticism. Results: Based on the EU ERA framework, we present an improved ERA concept that is system oriented with the GM plant at the centre and integrates a procedure for selection of testing organisms that do occur in the receiving environment. We also propose a hierarchical testing scheme from laboratory studies to field trials and we illustrate the outcomes for three different crop case examples. Conclusions and recommendations: Our proposed concept can alleviate a number of deficits identified in the current approach to ERA of GM plants. It allows the ERA to be tailored to the GM plant case and the receiving environment

Genomic Constitution of \u3cem\u3eFestulolium\u3c/em\u3e Varieties

Hybrids between species of ryegrass (Lolium) and fescue (Festuca) combine useful agronomical characteristics such as rapid establishment from seed and fodder quality from ryegrass and tolerance against abiotic and biotic stressses from fescue. The superior potential of hybrids has stimulated breeding programs generating so called Festulolium varieties. While the varieties have been evaluated extensively for their agronomic characteristics, little information is publicly available on their genomic constitution. The aim of our study was to analyse genomic constitution of a representative set of commercially available European Festulolium cultivars. To do this, we have employed genomic in situ hybridization (GISH)

Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. `Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).Peer reviewe

A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

Citation: Chapman, J. A., Mascher, M., Buluç, A., Barry, K., Georganas, E., Session, A., . . . Rokhsar, D. S. (2015). A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. Genome Biology, 16(1). doi:10.1186/s13059-015-0582-8Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population. © 2015 Chapman et al. licensee BioMed Central.Additional Authors: Muehlbauer, G. J.;Stein, N.;Rokhsar, D. S

Prehabilitation during neoadjuvant therapy in patients with cancer of the upper gastrointestinal tract and rectum—the study protocol

2025 Chmelova, Pastucha, Hudecek, Guran, Ciecotkova, Mart\uednek, Zubikova, Matlova, Dolezel, Salounova and Chmelo. Aims: The concept of prehabilitation, defined as interventions aimed at enhancing a patient\u27s functional capacity prior to an impending physiological stressor, may contribute to reduced postoperative morbidity and mortality. The study\u27s goal is to verify or refute the feasibility of a prehabilitation programme for two diagnostic patient groups during neoadjuvant chemo(radio)therapy, which is prescribed before the scheduled surgical procedure. This is a single group study design, with all patients enrolled receiving the intervention. Methods: This is an interventional feasibility study of a prehabilitation programme in the form of physical training conducted at home. The training consists of progressively dosed walking and strength exercises for selected muscle groups. Data will be monitored telemetrically and also through telephone contact with participants. Primary outcomes include: the percentage of patients interested in participating in the study out of all patients indicated for neoadjuvant therapy at University Hospital Ostrava during the observed period, the percentage of patients who complete the prehabilitation programme until the date of surgery and individual patient compliance. Secondary outcomes include physical fitness parameters obtained from cardiopulmonary exercise testing, grip strength measured by a dynamometer, changes in body composition, EORTC QLQ-C30 quality of life questionnaire, and a questionnaire on the subjective perception of the exercise programme. Both primary and secondary outcomes will be compared between study arms (two diagnostic groups). The study is registered at ClinicalTrials.gov, ID: NCT05646043. Conclusion: The results of this study can serve as a foundation for larger, multicentre, controlled studies in the future

Agency Staffing and Hospital Financial Performance: Insights and Implications

Rohit Pradhan,1 Bradley Beauvais,1 Zo Ramamonjiarivelo,1 Diane Dolezel,2 Dan Wood,3 Ramalingam Shanmugam1 1School of Health Administration, Texas State University, San Marcos, TX, 78666, USA; 2Department of Health Informatics and Information Management, Texas State University, Round Rock, TX, 78665, USA; 3Army-Baylor University MHA/MBA Program, U.S. Army Medical Center of Excellence, San Antonio, TX, 78234, USACorrespondence: Rohit Pradhan, ENC 264, School of Health Administration, Texas State University, 601 University Dr, San Marcos, TX, 78666, USA, Tel +1 (512) 245-6529, Email [email protected]: Staffing is critical to hospital performance. However, in recent years, hospitals have struggled with severe staffing shortages, forcing them to rely on expensive agency staff to meet urgent patient care needs. This substitution of agency staff for permanent employees has raised concerns over its potential impact on financial stability. This study investigated the association of agency labor with hospital financial performance.Methods: Utilizing tenets from agency theory and transaction cost theory, data for the calendar year 2022 for active short-term acute care hospitals (n=2771) in the United States were analyzed using multivariable linear regression analysis. Hospital financial performance was assessed using three variables: net patient revenue, operating revenue per bed, and operating expense per bed. The independent variable was agency labor cost, representing the total expenditure on agency labor. Additionally, organizational and market-level control variables that may independently affect hospital financial performance were included.Results: Our regression findings indicated that agency labor cost was significantly associated with all three dependent variables: net patient revenue (β = 0.224, p < 0.001), operating revenue per bed (β = 0.042, p < 0.001), and operating expense per bed (β = 0.032, p < 0.001).Discussion: The results indicated that increased agency labor was associated with higher revenues, but it also corresponded with increased expenses. Therefore, hospitals should strategically use agency staffing to meet immediate operational needs while remaining cognizant of its financial implications. The judicious use of agency labor can help hospitals balance the benefits of increased revenue against higher costs, while ensuring that they still meet immediate patient needs.Keywords: agency staff, contract labor, hospital financial performance, healthcare staffin