Attitudes towards the maned wolf amongst adolescents in the southeast of Brazil

The relationships between people and carnivores are a worldwide concern for the conservation of species and habitats. The maned wolf is an endangered, endemic canid inhabiting the southeast of Brazil - highly populated and rich in biodiversity and endemism. Strategies to conserve this key stone species may benefit the also declining Cerrado biome. The attitudes of teenagers towards wild carnivores are also of worldwide interest as future citizens and future decision makers. The present study investigates the attitudes of two age groups (12-13 and 16-17) towards the maned wolf. Questionnaires aimed to identify selected attitudes, beliefs and knowledge in relation to the maned wolf in urban areas of three locations in the São Paulo state. Responses were analysed according to age groups, gender, location and experiences concerning the maned wolf. Results suggest that positive attitudes declined with age; gender have very little effect on attitudes; zoos, as well as seeing the maned wolf in nature may increase support for the conservation of the species amongst the younger group; while identification with some maned wolf attributes amongst older teens may foster intentions to help the species. Conservation strategies and environmental education can help to provide early positive experiences of contact with the maned wolf and the Cerrado and be tailored to address attitudes in different age groups. This study can inform the planning of effective environmental educational strategies to foster long term support for the conservation of the maned wolf and the Cerrado

Implications of teenagers' attitudes toward maned wolf conservation in Brazil

The relationships between people and wild canids are a widespread concern for the conservation of species and habitats. The maned wolf Chrysocyon brachyurus is a Near Threatened species inhabiting South America. Strategies to conserve this keystone species may benefit the also-declining Cerrado biome. The attitudes of teenagers toward wild carnivores are also of worldwide interest as these youth are the future decision makers. We investigated selected attitudes, beliefs and knowledge in relation to the maned wolf of two age groups (12-13 and 16-17), using questionnaires delivered in three urban areas of the São Paulo state, a region highly populated and rich in biodiversity and endemism. Responses were analysed according to age groups, gender, location and experiences concerning the maned wolf. Results suggest that positive attitudes declined with age; gender has very little effect on attitudes; seeing the maned wolf in nature and zoos may increase support for the conservation and may facilitate learning about the species, while identification with some maned wolf attributes amongst older teens in the most urbanised areas may foster sympathy toward the species. Conservation strategies and environmental education can help to provide early positive experiences of contact with the maned wolf and the Cerrado, especially if they are tailored to address the variation we found in attitudes between different age groups. Research report Implications of teenagers' attitudes toward maned wolf conservation in Brazil. Available from

Contemporary outcome measures in acute stroke research: choice of primary outcome measure

BACKGROUND AND PURPOSE: The diversity of available outcome measures for acute stroke trials is challenging and implies that the scales may be imperfect. To assist researchers planning trials and to aid interpretation, this article reviews and makes recommendations on the available choices of scales. The aim is to identify an approach that will be universally accepted and that should be included in most acute trials, without seeking to restrict options for special circumstances. METHODS: The article considers outcome measures that have been widely used or are currently advised. It examines desirable properties for outcome measures such as validity, relevance, responsiveness, statistical properties, availability of training, cultural and language issues, resistance to comorbidity, as well as potential weaknesses. Tracking and agreement among outcomes are covered. RESULTS: Typical ranges of scores for the common scales are described, along with their statistical properties, which in turn influence optimal analytic techniques. The timing of recovery on scores and usual practice in trial design are considered. CONCLUSIONS: The preferred outcome measure for acute trials is the modified Rankin Scale, assessed at 3 months after stroke onset or later. The interview should be conducted by a certified rater and should involve both the patient and any relevant caregiver. Incremental benefits at any level of the modified Rankin Scale may be acceptable. The modified Rankin Scale is imperfect but should be retained in its present form for comparability with existing treatment comparisons. No second measure should be required, but correlations with supporting scales may be used to confirm consistency in direction of effects on other measures

PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens.

Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy analysis option. While a few bioinformatic tools have been developed for this purpose, their utility is still hindered either due to limited functionality or the requirement of bioinformatic expertise. To make sequencing data analysis of CRISPR/Cas9 screens more accessible to a wide range of scientists, we developed a Platform-independent Analysis of Pooled Screens using Python (PinAPL-Py), which is operated as an intuitive web-service. PinAPL-Py implements state-of-the-art tools and statistical models, assembled in a comprehensive workflow covering sequence quality control, automated sgRNA sequence extraction, alignment, sgRNA enrichment/depletion analysis and gene ranking. The workflow is set up to use a variety of popular sgRNA libraries as well as custom libraries that can be easily uploaded. Various analysis options are offered, suitable to analyze a large variety of CRISPR/Cas9 screening experiments. Analysis output includes ranked lists of sgRNAs and genes, and publication-ready plots. PinAPL-Py helps to advance genome-wide screening efforts by combining comprehensive functionality with user-friendly implementation. PinAPL-Py is freely accessible at http://pinapl-py.ucsd.edu with instructions and test datasets

The Stationary Phase Method for a Wave Packet in a Semiconductor Layered System. The applicability of the method

Using the formal analysis made by Bohm in his book, {\em "Quantum theory"}, Dover Publications Inc. New York (1979), to calculate approximately the phase time for a transmitted and the reflected wave packets through a potential barrier, we calculate the phase time for a semiconductor system formed by different mesoscopic layers. The transmitted and the reflected wave packets are analyzed and the applicability of this procedure, based on the stationary phase of a wave packet, is considered in different conditions. For the applicability of the stationary phase method an expression is obtained in the case of the transmitted wave depending only on the derivatives of the phase, up to third order. This condition indicates whether the parameters of the system allow to define the wave packet by its leading term. The case of a multiple barrier systems is shown as an illustration of the results. This formalism includes the use of the Transfer Matrix to describe the central stratum, whether it is formed by one layer (the single barrier case), or two barriers and an inner well (the DBRT system), but one can assume that this stratum can be comprise of any number or any kind of semiconductor layers.Comment: 15 pages, 4 figures although figure 4 has 5 graph

Unravelling the geographical palimpsest through fieldwork: discovering a sense of place

Fieldwork enables students to gain a greater understanding of the people and places that they encounter. Urban areas are popular destinations for fieldwork because they present a landscape that is in a continual state of change. Yet, as this article indicates, the past is ever present in the urban landscape as each place can be regarded as a palimpsest, where layers of history, geography, culture and politics co-exist. Through active participation in fieldwork, students have the potential to unravel this palimpsest and discover a deeper sense of that place. The example used here reflects upon urban fieldwork in Barcelona as experienced by a group of undergraduate students who, as they carry out and reflect upon their field work, benefit from gaining a sense of place informed by a range of geographical processes and meanings. The article concludes with a series of suggestions for those embarking on fieldwork and wishing to unravel the geographical palimpsest

Gaining a “sense of place”: students’ affective experiences of place leading to transformative learning on international fieldwork

This paper reveals the extent to which undergraduate students demonstrate transformative learning whilst on international fieldwork in Barcelona, Spain. Groups of students create a series of discrete active learning situations that allow them and their peers to engage more fully with their locale and in turn experience a deeper understanding of “place”. Reflective field trip notebooks are used to capture the experiences of students. Results show that through the use of the affective domain (Krathwohl’s taxonomy) and applying cyclic experiential learning (Kolb) combined with critical reflection (Mezirow), students demonstrate progression and, in some cases, regression along Krathwohl’s taxonomy

Further studies on the iodine concentration of conventional, organic and UHT semi-skimmed milk at retail in the UK

Milk is the largest source of iodine in UK diets and earlier studies showed organic summer and winter milk to be significantly lower in iodine than conventional milk. One study also showed UHT milk to have lower iodine concentration. The study on winter and UHT milk was small and accordingly a new study is reported here involving conventional, organic and UHT semi-skimmed milk from four supermarkets over a six-month period in summer and winter in two regions of the UK. The results showed organic milk to be 44% lower in iodine than conventional milk (427 vs. 241 mg/L, P < 0.001) and UHT milk was 27% lower in iodine than conventional milk (427 vs. 314 mg/L, P < 0.001) although the differences tended to be less in the summer. The results indicate that replacement of conventional milk by organic or UHT milk will increase the risk of sub-optimal iodine status especially for pregnant/lactating women

Further studies on the iodine concentration of conventional, organic and UHT semi-skimmed milk at retail in the UK

Milk is the largest source of iodine in UK diets and earlier studies showed organic summer and winter milk to be significantly lower in iodine than conventional milk. One study also showed UHT milk to have lower iodine concentration. The study on winter and UHT milk was small and accordingly a new study is reported here involving conventional, organic and UHT semi-skimmed milk from four supermarkets over a six-month period in summer and winter in two regions of the UK. The results showed organic milk to be 44% lower in iodine than conventional milk (427 vs. 241 mg/L, P < 0.001) and UHT milk was 27% lower in iodine than conventional milk (427 vs. 314 mg/L, P < 0.001) although the differences tended to be less in the summer. The results indicate that replacement of conventional milk by organic or UHT milk will increase the risk of sub-optimal iodine status especially for pregnant/lactating women

Are fluoride levels in drinking water associated with hypothyroidism prevalence in England? A large observational study of GP practice data and fluoride levels in drinking water

Background While previous research has suggested that there is an association between fluoride ingestion and the incidence of hypothyroidism, few population level studies have been undertaken. In England, approximately 10% of the population live in areas with community fluoridation schemes and hypothyroidism prevalence can be assessed from general practice data. This observational study examines the association between levels of fluoride in water supplies with practice level hypothyroidism prevalence. Methods We used a cross-sectional study design using secondary data to develop binary logistic regression models of predictive factors for hypothyroidism prevalence at practice level using 2012 data on fluoride levels in drinking water, 2012/2013 Quality and Outcomes Framework (QOF) diagnosed hypothyroidism prevalence data, 2013 General Practitioner registered patient numbers and 2012 practice level Index of Multiple Deprivation scores. Findings We found that higher levels of fluoride in drinking water provide a useful contribution for predicting prevalence of hypothyroidism. We found that practices located in the West Midlands (a wholly fluoridated area) are nearly twice as likely to report high hypothyroidism prevalence in comparison to Greater Manchester (non-fluoridated area). Interpretation In many areas of the world, hypothyroidism is a major health concern and in addition to other factors—such as iodine deficiency—fluoride exposure should be considered as a contributing factor. The findings of the study raise particular concerns about the validity of community fluoridation as a safe public health measure