Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina ; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina ; ArgentinaFil: Cayrol, Maria Florencia. Pontificia Universidad Católica Argentina ; ArgentinaFil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina ; ArgentinaFil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Colombo, L.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Pontificia Universidad Católica Argentina ; ArgentinaFil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentin

Fungal home-made enzymatic cocktails for apple juice clarification

Sugarcane bagasse and cassava bagasse are lignocellulosic industrialresidues that can be used as cheap substrates for organisms? growth. Theselignocellulosic residues are also suitable for inducing enzyme secretion thatcan be applied in different bioprocesses such as juices clarification. The goalof our work was to use Aspergillus niger LBM 134 to produce xylanases,characterize them and employ these enzymes in the clarification of applejuice and pulp. A. niger LBM 134 was isolated from a natural environmentand grown on sugarcane bagasse and cassava bagasse. The highestendoxylanase and β-xylosidase activity were 144 ± 5,65 and 0,74 ± 0,05 UmL-1, respectively (p<0.05). The optimum activity and high stability atacidic pH values make these enzymes suitable for biotechnologicalapplications in juice industries. The enzymatic cocktails produced by thefungus grown on cassava bagasse reached the major clarification of applejuice (36,66 ± 4,01%) and pulp (60,15 ± 5,63%) (p<0.05). Theseclarification percentages were due to the hydrolysis of hemicellulosicmaterial carried out by the xylanases from A. niger LBM 134.Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Coniglio, Romina Olga. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Bordaquievich, Mayra Florencia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Villalba, L. L.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

Frequência de comportamentos associados ao plágio estudantil em uma universidade em Porto Rico

Behaviors associated with plagiarism committed by students in universities in different countries have been reported in plentiful research over time. This article presents the results of the second cross-sectional survey conducted at a public university in Puerto Rico to pin down the frequency of behaviors associated with academic dishonesty and, in particular, plagiarism among students in undergraduate programs. Three hundred and sixty students answered the Academic Honesty Questionnaire-Revised. As a result, the frequency and percentage of plagiarism-related behaviors admitted and observed in the institutions' students were obtained. These are the three most frequently admitted behaviors: (a) paraphrasing or using ideas or words of a person or source without indicating it; (b) inserting in the bibliography references that were not consulted; (c) verbatim copying information from any printed material without citing its author or indicating it in a footnote or elsewhere in a text. A higher percentage of the students observed behaviors associated with plagiarism: (a) copying quotes, words, phrases, or paragraphs from printed works, without indicating the source; (b) copying and pasting into a written work, phrases, sentences, parts of documents or pages copied from the Internet, without indicating the source; and (c) produce a written work using parts copied verbatim from Internet pages or documents. Like other studies, the results indicate that they attribute a higher frequency to acts of plagiarism observed in their peers. In addition, they resort to both printed and Internet sources to copy information without indicating the source and self-plagiarism of previous works.Los comportamientos asociados con el plagio que comete el estudiantado en universidades en distintos países se han reportado múltiples investigaciones a lo largo del tiempo. Este artículo presenta los resultados de la segunda encuesta transversal realizada en una universidad pública en Puerto Rico para determinar la frecuencia de comportamientos asociados con la deshonestidad académica y, en particular, los de plagio de estudiantes en programas subgraduados. Trescientos sesenta estudiantes contestaron el Cuestionario de Honestidad Académica-Revisado. Se obtuvo la frecuencia y el porcentaje de los comportamientos asociados al plagio admitidos y observados en el estudiantado de la institución. Los tres con mayor frecuencia de admisión fueron: (a) parafrasear o usar ideas o palabras de una persona o fuente, sin indicarla; (b) rellenar una bibliografía con referencias que no fueron consultadas; y (c) copiar literalmente información de algún material impreso, sin citar su autor o autora o indicarlo en una nota al calce u otro lugar del trabajo. Un porcentaje mayor del grupo de estudiantes observó comportamientos asociados al plagio: (a) copiar citas, palabras, frases o párrafos de trabajos impresos, sin indicar la fuente; (b) copiar y pegar en un trabajo escrito frases, oraciones, partes de documentos o páginas copiadas de la Internet, sin indicar la fuente; y (c) elaborar un trabajo escrito usando partes copiadas literalmente de páginas o documentos de la Internet. Al igual que otros estudios, los resultados apuntan a que atribuyen mayor frecuencia a los actos de plagio observados en sus pares; además, recurren tanto a fuentes impresas y de Internet para copiar información, sin indicar la fuente y al autoplagio de trabajos previos.Os comportamentos associados ao plágio estudantil em universidades de diferentes países têm sido relatados em múltiplos estudos de pesquisa ao longo do tempo. Este artigo apresenta os resultados da segunda pesquisa transversal realizada em uma universidade pública em Porto Rico para determinar a frequência de comportamentos associados à desonestidade acadêmica e, em particular, ao plágio estudantil em programas de pré-graduação. Trezentos e sessenta estudantes completaram o Questionário de Honestidade Acadêmica-Revisado. Obteve-se a frequência e o percentual de comportamentos associados ao plágio admitidos e observados no corpo discente da instituição. As três mais frequentemente admitidas foram: (a) parafrasear ou usar ideias ou palavras de uma pessoa ou fonte, sem indicá-la; (b) preencher uma bibliografia com referências que não foram consultadas; e (c) copiar literalmente informações de algum material impresso, sem citar seu autor ou autora ou indicá-lo em uma nota de rodapé ou em outro lugar do trabalho. Em uma porcentagem maior do grupo de estudantes foi observado comportamentos associados ao plágio: (a) copiar citações, palavras, frases ou parágrafos de trabalhos impressos sem indicar a fonte; (b) copiar e colar frases, sentenças, partes de documentos ou páginas copiadas da Internet, sem indicar a fonte; e (c) produzir um trabalho escrito usando partes copiadas literalmente de páginas ou documentos da Internet. Semelhante a outros estudos, os resultados sugerem que eles atribuem maior frequência aos atos de plágio observados em seus pares; além disso, eles utilizam fontes impressas e da Internet para copiar informações, sem indicar a fonte, e o autoplágio dos trabalhos prévios

Claves para una lectura contemporánea de los derechos humanos en el contexto del actual orden político mundial

This article looks forward to promote critical thinking about controversial themes such as violence, war, terrorism and strategic ways of resistance of victims. These are terms of actual political world order, powerful countries have build their kingdoms on, in a culture where violent procedures are considered successful. Hints are given throughout the reading, about how to understand human rights, specifically the right of resistance in the ethical and political judgement over violence. This is why, victims and their experience are the starting point of all critics related to Totality as current system, because it has become “absolutely intolerable”.En este artículo se lleva a cabo una reflexión crítica sobre la concepción que ha construido el pensamiento único de las potencias hegemónicas acerca de cuestiones tan controvertidas e inciertas como la violencia, la guerra, el terrorismo y las formas estratégicas de resistencia de las víctimas en el ejercicio del poder en una cultura de los procedimientos violentos como procedimientos triunfantes en el contexto del actual orden político mundial. En el artículo se enuncian las claves para una lectura y una mirada nueva sobre el papel que cumplen los Derechos Humanos, específicamente el derecho de resistencia en la tensión inconciliable entre ética y política respecto del juicio sobre la violencia. Por ello, la víctima y su experiencia es el punto de partida para llevar a cabo la crítica de la Totalidad existente como sistema vigente, debido a que “ha llegado a ser absolutamente intolerable”

Frequência de comportamentos associados ao plágio estudantil em uma universidade em Porto Rico

Behaviors associated with plagiarism committed by students in universities in different countries have been reported in plentiful research over time. This article presents the results of the second cross-sectional survey conducted at a public university in Puerto Rico to pin down the frequency of behaviors associated with academic dishonesty and, in particular, plagiarism among students in undergraduate programs. Three hundred and sixty students answered the Academic Honesty Questionnaire-Revised. As a result, the frequency and percentage of plagiarism-related behaviors admitted and observed in the institutions' students were obtained. These are the three most frequently admitted behaviors: (a) paraphrasing or using ideas or words of a person or source without indicating it; (b) inserting in the bibliography references that were not consulted; (c) verbatim copying information from any printed material without citing its author or indicating it in a footnote or elsewhere in a text. A higher percentage of the students observed behaviors associated with plagiarism: (a) copying quotes, words, phrases, or paragraphs from printed works, without indicating the source; (b) copying and pasting into a written work, phrases, sentences, parts of documents or pages copied from the Internet, without indicating the source; and (c) produce a written work using parts copied verbatim from Internet pages or documents. Like other studies, the results indicate that they attribute a higher frequency to acts of plagiarism observed in their peers. In addition, they resort to both printed and Internet sources to copy information without indicating the source and self-plagiarism of previous works.Los comportamientos asociados con el plagio que comete el estudiantado en universidades en distintos países se han reportado múltiples investigaciones a lo largo del tiempo. Este artículo presenta los resultados de la segunda encuesta transversal realizada en una universidad pública en Puerto Rico para determinar la frecuencia de comportamientos asociados con la deshonestidad académica y, en particular, los de plagio de estudiantes en programas subgraduados. Trescientos sesenta estudiantes contestaron el Cuestionario de Honestidad Académica-Revisado. Se obtuvo la frecuencia y el porcentaje de los comportamientos asociados al plagio admitidos y observados en el estudiantado de la institución. Los tres con mayor frecuencia de admisión fueron: (a) parafrasear o usar ideas o palabras de una persona o fuente, sin indicarla; (b) rellenar una bibliografía con referencias que no fueron consultadas; y (c) copiar literalmente información de algún material impreso, sin citar su autor o autora o indicarlo en una nota al calce u otro lugar del trabajo. Un porcentaje mayor del grupo de estudiantes observó comportamientos asociados al plagio: (a) copiar citas, palabras, frases o párrafos de trabajos impresos, sin indicar la fuente; (b) copiar y pegar en un trabajo escrito frases, oraciones, partes de documentos o páginas copiadas de la Internet, sin indicar la fuente; y (c) elaborar un trabajo escrito usando partes copiadas literalmente de páginas o documentos de la Internet. Al igual que otros estudios, los resultados apuntan a que atribuyen mayor frecuencia a los actos de plagio observados en sus pares; además, recurren tanto a fuentes impresas y de Internet para copiar información, sin indicar la fuente y al autoplagio de trabajos previos.Os comportamentos associados ao plágio estudantil em universidades de diferentes países têm sido relatados em múltiplos estudos de pesquisa ao longo do tempo. Este artigo apresenta os resultados da segunda pesquisa transversal realizada em uma universidade pública em Porto Rico para determinar a frequência de comportamentos associados à desonestidade acadêmica e, em particular, ao plágio estudantil em programas de pré-graduação. Trezentos e sessenta estudantes completaram o Questionário de Honestidade Acadêmica-Revisado. Obteve-se a frequência e o percentual de comportamentos associados ao plágio admitidos e observados no corpo discente da instituição. As três mais frequentemente admitidas foram: (a) parafrasear ou usar ideias ou palavras de uma pessoa ou fonte, sem indicá-la; (b) preencher uma bibliografia com referências que não foram consultadas; e (c) copiar literalmente informações de algum material impresso, sem citar seu autor ou autora ou indicá-lo em uma nota de rodapé ou em outro lugar do trabalho. Em uma porcentagem maior do grupo de estudantes foi observado comportamentos associados ao plágio: (a) copiar citações, palavras, frases ou parágrafos de trabalhos impressos sem indicar a fonte; (b) copiar e colar frases, sentenças, partes de documentos ou páginas copiadas da Internet, sem indicar a fonte; e (c) produzir um trabalho escrito usando partes copiadas literalmente de páginas ou documentos da Internet. Semelhante a outros estudos, os resultados sugerem que eles atribuem maior frequência aos atos de plágio observados em seus pares; além disso, eles utilizam fontes impressas e da Internet para copiar informações, sem indicar a fonte, e o autoplágio dos trabalhos prévios

Acinetobacter Lwofii, an unusual cause of infectious pericarditis complicated with cardiac tamponade: a case report

Bacterial pericarditis can be considered a rare pathology, usually associated with cardiac procedures and, to a lesser extent, with immunosuppression and chronic diseases. The importance of its knowledge lies in the fact that mortality can reach up to 100% in untreated patients. Once diagnosed, pericardiocentesis and administration of intravenous antimicrobial therapy are mandatory for the prevention of its complications, which include cardiac tamponade and sepsis. Here we present an exceptional case of infectious pericarditis due to Acinetobacter Lwoffii in an older adult, which was complicated by pericardial effusion and cardiac tamponade

Use of time-of-flight mass spectrometry for large screening of organic pollutants in surface waters and soils from a rice production area in Colombia

The irrigate district of Usosaldaña, an important agricultural area in Colombia mainly devoted to rice crop production, is subjected to an intensive use of pesticides. Monitoring these compounds is necessary to know the impact of phytosanitary products in the different environmental compartments. In this work, surface water and soil samples from different sites of this area have been analyzed by applying an analytical methodology for large screening based on the use of time-of-flight mass spectrometry (TOF MS) hyphenated to liquid chromatography (LC) and gas chromatography (GC). Several pesticides were detected and unequivocally identified, such as the herbicides atrazine, diuron or clomazone. Some of their main metabolites and/or transformation products (TPs) like deethylatrazine (DEA), deisopropylatrazine (DIA) and 3,4-dichloroaniline were also identified in the samples. Among fungicides, carbendazim, azoxystrobin, propiconazole and epoxiconazole were the most frequently detected. Insecticides such as thiacloprid, or p,p′-DDT metabolites (p,p′-DDD and p,p′-DDE) were also found. Thanks to the accurate-mass full-spectrum acquisition in TOF MS it was feasible to widen the number of compounds to be investigated to other families of contaminants. This allowed the detection of emerging contaminants, such as the antioxidant 3,5-di-tertbutyl-4-hydroxy-toluene (BHT), its metabolite 3,5-di-tert-butyl-4-hydroxy-benzaldehyde (BHT-CHO), or the solar filter benzophenone, among other

Assessment of static posturography and pedobarography for the detection of unilateral forelimb lameness in ponies

Background: Static posturography and pedobarography are based on the detection of postural imbalance and, consequently, the pressure redistribution between limbs in lame subjects. These techniques have proven to be useful for the detection of lameness in humans and dogs. The main objective of this study was to test the suitability of static posturography and pedobarography in diagnosing lameness in ponies. A pressure platform was used to obtain postural data (statokinesiograms, mean X and Y, length, LFS ratio, and mean velocity) from 10 sound ponies and 7 ponies with unilateral forelimb lameness. Static pedobarographic data (pressure distribution, mean pressure, and peak pressure) were also collected and compared with force plate data (peak vertical force and vertical impulse) obtained from the same animals at the walk. Results: Significant differences were seen between lame and sound ponies for almost all evaluated parameters. With this sample size, differences between lame and sound limbs/groups were detected with a statistical power of 90%, except for mean X and Y. Conclusions: Static posturography and pedobarography provide a complementary approach for lameness detection in equids

Epigenetic variability in the genetically uniform forest tree species Pinus pinea L

There is an increasing interest in understanding the role of epigenetic variability in forest species and how it may contribute to their rapid adaptation to changing environments. In this study we have conducted a genome-wide analysis of cytosine methylation pattern in Pinus pinea, a species characterized by very low levels of genetic variation and a remarkable degree of phenotypic plasticity. DNA methylation profiles of different vegetatively propagated trees from representative natural Spanish populations of P. pinea were analyzed with the Methylation Sensitive Amplified Polymorphism (MSAP) technique. A high degree of cytosine methylation was detected (64.36% of all scored DNA fragments). Furthermore, high levels of epigenetic variation were observed among the studied individuals. This high epigenetic variation found in P. pinea contrasted with the lack of genetic variation based on Amplified Fragment Length Polymorphism (AFLP) data. In this manner, variable epigenetic markers clearly discriminate individuals and differentiates two well represented populations while the lack of genetic variation revealed with the AFLP markers fail to differentiate at both, individual or population levels. In addition, the use of different replicated trees allowed identifying common polymorphic methylation sensitive MSAP markers among replicates of a given propagated tree. This set of MSAPs allowed discrimination of the 70% of the analyzed trees

Aplicación de índices de vegetación para evaluar la falta de producción de pastos y montaneras en dehesas.

El ganado porcino ibérico aprovecha los recursos naturales de la dehesa mediante montanera, principalmente la bellota y los pastos existentes. La línea 133 de los seguros agrarios españoles recoge el seguro de compensación por pérdida de pastos, solo para bovino reproductor y de lidia, ovino, caprino y equino, no incluyen los cerdos en montanera. Emplea un Índice de Vegetación de la Diferencia Normalizada (NDVI) medido por satélite sobre pastos desarbolados. El objetivo es comprobar si se puede utilizar un índice de vegetación para estimar la producción de pasto y bellota. Se han tomado datos del aforo de montaneras desde 1999 al 2005, y del pasto en dehesas de Salamanca (Vitigudino), Cáceres (Trujillo) y Córdoba (Pozoblanco) durante 2010 al 2012. Con los datos de 2010 y 2011 se estableció una función de producción del pasto fresco en función del NDVI, mostrando un coeficiente de correlación de 0,975, altamente significativa. Los datos obtenidos en 2012 se utilizaron para validar la función de producción de pasto fresco. La comparación entre los valores observados y simulados para 2012 ha mostrado un coeficiente de correlación de 0,734. Como conclusión, el NDVI puede ser un buen estimador de la cantidad de pasto fresco en dehesas españolas