Disruption of exocytosis in sympathoadrenal chromaffin cells from mouse models of neurodegenerative diseases

Synaptic disruption and altered neurotransmitter release occurs in the brains of patients and in murine models of neurodegenerative diseases (NDDs). During the last few years, evidence has accumulated suggesting that the sympathoadrenal axis is also affected as disease progresses. Here, we review a few studies done in adrenal medullary chromaffin cells (CCs), that are considered as the amplifying arm of the sympathetic nervous system; the sudden fast exocytotic release of their catecholamines—stored in noradrenergic and adrenergic cells—plays a fundamental role in the stress fight-or-flight response. Bulk exocytosis and the fine kinetics of single-vesicle exocytotic events have been studied in mouse models carrying a mutation linked to NDDs. For instance, in R6/1 mouse models of Huntington’s disease (HD), mutated huntingtin is overexpressed in CCs; this causes decreased quantal secretion, smaller quantal size and faster kinetics of the exocytotic fusion pore, pore expansion, and closure. This was accompanied by decreased sodium current, decreased acetylcholine-evoked action potentials, and attenuated [Ca2+]c transients with faster Ca2+ clearance. In the SOD1G93A mouse model of amyotrophic lateral sclerosis (ALS), CCs exhibited secretory single-vesicle spikes with a slower release rate but higher exocytosis. Finally, in the APP/PS1 mouse model of Alzheimer’s disease (AD), the stabilization, expansion, and closure of the fusion pore was faster, but the secretion was attenuated. Additionally, α-synuclein that is associated with Parkinson’s disease (PD) decreases exocytosis and promotes fusion pore dilation in adrenal CCs. Furthermore, Huntington-associated protein 1 (HAP1) interacts with the huntingtin that, when mutated, causes Huntington’s disease (HD); HAP1 reduces full fusion exocytosis by affecting vesicle docking and controlling fusion pore stabilization. The alterations described here are consistent with the hypothesis that central alterations undergone in various NDDs are also manifested at the peripheral sympathoadrenal axis to impair the stress fight-or-flight response in patients suffering from those diseases. Such alterations may occur: (i) primarily by the expression of mutated disease proteins in CCs; (ii) secondarily to stress adaptation imposed by disease progression and the limitations of patient autonomy.Spanish Ministry of Science, Technology and Innovation SAF2016-78892R; EU Horizon 2020 Research and Innovation Programme under Marie Slodowska-Curie Grant Agreement N. 766124. We acknowledge the continuous support of Fundación Teófilo Hernando

Structure and genetic diversity in wild and cultivated populations of Zapote mamey (Pouteria sapota, Sapotaceae) from southeastern Mexico: its putative domestication center

Tropical fruit trees are an important component of the human diet; however, little is known about their genetic diversity levels. Zapote mamey (Pouteria sapota) is a tree native to southeastern Mexico and Central America, and Mexico is the leading producer in the world. Studies of the genetic diversity of Zapote mamey have been based on cultivated materials using morphological and biochemical characterization or dominant molecular markers. To gain a deeper understanding about the conservation status of Zapote mamey in its center of origin and domestication, we collected 188 individuals from eight wild and five cultivated populations in southeastern Mexico and characterized them using eight microsatellite loci. STRUCTURE, 3D-PCoA, and neighbor-joining analyses showed three groups in the wild gene pool and one group in the cultivated gene pool. FST values were significant between wild and cultivated gene pools, among the four groups observed and among the 13 populations collected (0.13, 0.25, and 0.36, respectively). Overall, we found low levels of genetic diversity (A = 2.77, HO = 0.29, HE = 0.39), permutation tests did not show significant differences between wild and cultivated gene pools. The Garza–Williamson index showed low values in both gene pools (wild = 0.16, cultivated = 0.11) and the Bottleneck program indicated a decrease in genetic diversity in both gene pools (wild, P = 0.027; cultivated, P = 0.054); both analyses suggest a potential genetic bottleneck within this species. This study can help to generate adequate sampling techniques and to develop effective management strategies for Zapote mamey of southeastern Mexico

Real-Time and Low-Cost Sensing Technique Based on Photonic Bandgap Structures

This paper was published in OPTICS LETTERS and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OL.36.002707. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law[EN] A technique for the development of low-cost and high-sensitivity photonic biosensing devices is proposed and experimentally demonstrated. In this technique, a photonic bandgap structure is used as transducer, but its readout is performed by simply using a broadband source, an optical filter, and a power meter, without the need of obtaining the transmission spectrum of the structure; thus, a really low-cost system and real-time results are achieved. Experimental results show that it is possible to detect very low refractive index variations, achieving a detection limit below 2 x 10(-6) refractive index units using this low-cost measuring technique. (C) 2011 Optical Society of America[This work was funded by the Spanish Ministerio de Ciencia e Innovacion (MICINN) under contracts TEC2008-06333, JCI-009-5805, and TEC2008-05490. Support by the Universidad Politecnica de Valencia through program PAID-06-09 and the Conselleria d'Educacio through program GV-2010-031 is acknowledged.García Castelló, J.; Toccafondo, V.; Pérez Millán, P.; Sánchez Losilla, N.; Cruz, JL.; Andres, MV.; García-Rupérez, J. (2011). Real-Time and Low-Cost Sensing Technique Based on Photonic Bandgap Structures. Optics Letters. 36(14):2707-2709. https://doi.org/10.1364/OL.36.002707S270727093614Fan, X., White, I. M., Shopova, S. I., Zhu, H., Suter, J. D., & Sun, Y. (2008). Sensitive optical biosensors for unlabeled targets: A review. Analytica Chimica Acta, 620(1-2), 8-26. doi:10.1016/j.aca.2008.05.022Homola, J., Yee, S. S., & Gauglitz, G. (1999). Surface plasmon resonance sensors: review. Sensors and Actuators B: Chemical, 54(1-2), 3-15. doi:10.1016/s0925-4005(98)00321-9Kersey, A. D., Davis, M. A., Patrick, H. J., LeBlanc, M., Koo, K. P., Askins, C. G., … Friebele, E. J. (1997). Fiber grating sensors. Journal of Lightwave Technology, 15(8), 1442-1463. doi:10.1109/50.618377De Vos, K., Bartolozzi, I., Schacht, E., Bienstman, P., & Baets, R. (2007). Silicon-on-Insulator microring resonator for sensitive and label-free biosensing. Optics Express, 15(12), 7610. doi:10.1364/oe.15.007610Iqbal, M., Gleeson, M. A., Spaugh, B., Tybor, F., Gunn, W. G., Hochberg, M., … Gunn, L. C. (2010). Label-Free Biosensor Arrays Based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation. IEEE Journal of Selected Topics in Quantum Electronics, 16(3), 654-661. doi:10.1109/jstqe.2009.2032510Xu, D.-X., Vachon, M., Densmore, A., Ma, R., Delâge, A., Janz, S., … Schmid, J. H. (2010). Label-free biosensor array based on silicon-on-insulator ring resonators addressed using a WDM approach. Optics Letters, 35(16), 2771. doi:10.1364/ol.35.002771Skivesen, N., Têtu, A., Kristensen, M., Kjems, J., Frandsen, L. H., & Borel, P. I. (2007). Photonic-crystal waveguide biosensor. Optics Express, 15(6), 3169. doi:10.1364/oe.15.003169Lee, M. R., & Fauchet, P. M. (2007). Nanoscale microcavity sensor for single particle detection. Optics Letters, 32(22), 3284. doi:10.1364/ol.32.003284García-Rupérez, J., Toccafondo, V., Bañuls, M. J., Castelló, J. G., Griol, A., Peransi-Llopis, S., & Maquieira, Á. (2010). Label-free antibody detection using band edge fringes in SOI planar photonic crystal waveguides in the slow-light regime. Optics Express, 18(23), 24276. doi:10.1364/oe.18.024276Toccafondo, V., García-Rupérez, J., Bañuls, M. J., Griol, A., Castelló, J. G., Peransi-Llopis, S., & Maquieira, A. (2010). Single-strand DNA detection using a planar photonic-crystal-waveguide-based sensor. Optics Letters, 35(21), 3673. doi:10.1364/ol.35.003673Luff, B. J., Wilson, R., Schiffrin, D. J., Harris, R. D., & Wilkinson, J. S. (1996). Integrated-optical directional coupler biosensor. Optics Letters, 21(8), 618. doi:10.1364/ol.21.000618Sepúlveda, B., Río, J. S. del, Moreno, M., Blanco, F. J., Mayora, K., Domínguez, C., & Lechuga, L. M. (2006). Optical biosensor microsystems based on the integration of highly sensitive Mach–Zehnder interferometer devices. Journal of Optics A: Pure and Applied Optics, 8(7), S561-S566. doi:10.1088/1464-4258/8/7/s41Densmore, A., Vachon, M., Xu, D.-X., Janz, S., Ma, R., Li, Y.-H., … Schmid, J. H. (2009). Silicon photonic wire biosensor array for multiplexed real-time and label-free molecular detection. Optics Letters, 34(23), 3598. doi:10.1364/ol.34.003598Povinelli, M. L., Johnson, S. G., & Joannopoulos, J. D. (2005). Slow-light, band-edge waveguides for tunable time delays. Optics Express, 13(18), 7145. doi:10.1364/opex.13.007145Garcia, J., Sanchis, P., Martinez, A., & Marti, J. (2008). 1D periodic structures for slow-wave induced non-linearity enhancement. Optics Express, 16(5), 3146. doi:10.1364/oe.16.003146Pérez-Millán, P., Torres-Peiró, S., Cruz, J. L., & Andrés, M. V. (2008). Fabrication of chirped fiber Bragg gratings by simple combination of stretching movements. Optical Fiber Technology, 14(1), 49-53. doi:10.1016/j.yofte.2007.07.00

Multiscale understanding of tricalcium silicate hydration reactions

Tricalcium silicate, the main constituent of Portland cement, hydrates to produce crystalline calcium hydroxide and calcium-silicate-hydrates (C-S-H) nanocrystalline gel. This hydration reaction is poorly understood at the nanoscale. The understanding of atomic arrangement in nanocrystalline phases is intrinsically complicated and this challenge is exacerbated by the presence of additional crystalline phase(s). Here, we use calorimetry and synchrotron X-ray powder diffraction to quantitatively follow tricalcium silicate hydration process: i) its dissolution, ii) portlandite crystallization and iii) C-S-H gel precipitation. Chiefly, synchrotron pair distribution function (PDF) allows to identify a defective clinotobermorite, Ca11Si9O28(OH)2.8.5H2O, as the nanocrystalline component of C-S-H. Furthermore, PDF analysis also indicates that C-S-H gel contains monolayer calcium hydroxide which is stretched as recently predicted by first principles calculations. These outcomes, plus additional laboratory characterization, yielded a multiscale picture for C-S-H nanocomposite gel which explains the observed densities and Ca/Si atomic ratios at the nano- and meso- scales.This work has been supported by Spanish MINECO through BIA2014-57658-C2-2-R, which is co-funded by FEDER, BIA2014-57658-C2-1-R and I3 (IEDI-2016-0079) grants. We also thank CELLS-ALBA (Barcelona, Spain) for providing synchrotron beam time at BL04-MSPD beamline

Preventive conservation plan for library buildings in La Plata, Argentina

This summary shows the advances of a project called “Development of building environmental management and continuous monitoring for preventive conservation in UNLP libraries” which is now taking place in the libraries of the National University of La Plata. It is essential to know the hygrothermal condition inside the book deposits, as one of the first preventive measures to become aware of the state of conservation of the books. The aim of the study is to propose a systematic environmental monitoring method to verify compatibility between measured parameters and optimal preservation conditions. Annual monitoring campaign was performed in twelve libraries with diverse results according to the characteristics of the envelope and HVAC systems. It is the first time that a formal monitoring campaign has taken place in these library buildings. We met staff well disposed and eager to achieve better results. We provide a standard report model suitable for the institutions as a decision-making tool, with simultaneous analysis of temperature and relative humidity, according to the local climate.Facultad de Arquitectura y Urbanism

Intersymbol and Intercarrier Interference in OFDM Systems: Unified Formulation and Analysis

A unified matrix formulation is presented for the analysis of intersymbol and intercarrier interference in orthogonal frequency-division multiplexing (OFDM) systems. The proposed formulation relies on six parameters and allows studying various schemes, including those with windowing in the transmitter and/or in the receiver (called windowed OFDM systems), which may add cyclic suffix and/or cyclic prefix (CP), besides the conventional CP-OFDM. The proposed framework encompasses seven different OFDM systems. It considers the overlap-and-add procedure performed in the transmitter of windowed OFDM systems, being jointly formulated with the channel convolution. The intersymbol and intercarrier interference, caused when the order of the channel impulse response is higher than the number of CP samples, is characterized. A new equivalent channel matrix that is useful for calculating both the received signal and the interference power is defined and characterized. Unlike previous works, this new channel matrix has no restrictions on the length of the channel impulse response, which means that the study is not constrained to the particular case of two or three data blocks interfering in the received signal. Theoretical expressions for the powers of three different kinds of interference are derived. These expressions allow calculating the signal-to-interference-plus-noise ratio, useful for computing the data rate of each OFDM system. The proposed formulation is applied to realistic examples, showing its effectiveness through comparisons based on numerical performance assessments of the considered OFDM systems

Aspectos a considerar en los cálculos de efectividad de una cobertura de valor razonable en donde el swap de tasa de interés intercambia una tasa flotante por otra tasa flotante

Key words: Bulletin C-10, effectiveness testing, hedge accounting, swapsAbstract. This article analyzes the hedge accounting of fair value hedge on a variable-tovariable swap. Following the effectiveness valuation procedure established in Bulletin C-10 “Derivative Financial Instruments and Hedge Activities” under Mexican GAAP will generate distorted effectiveness outcome even though the critical terms between the hedge item and the hedging instrument are the same in all aspects. To show this effect we present ahypothetic example for a fair value hedge relationship LIBOR-TIIE. Our conclusion is that this distortion is due to a valuation effect known as “pull to par” especially when the coupon reset dates of the hedge item and the hedging instrument does not match with the valuation date. Finally, it propones alternative ways to book this kind of transactions that eliminate thetemporal distortion that this pull to par effect generates.Palabras claves: Boletín C-10, contabilidad de coberturas, evaluación de efectividad, swapsResumen. En este artículo se analiza que la contabilización de una cobertura de valor razonable de un swap de tasa de interés con intercambios de variable por variable, siguiendo el procedimiento de evaluación de efectividad establecido en el Boletín C-10 “Instrumentos Financieros Derivados y Operaciones de Cobertura” de las Normas de Información Financieras, generará resultados de efectividad distorsionados aún y cuando los términos críticos entre la Posición Primaria sujeta a cobertura y el Instrumento Financiero Derivado (IFD) utilizado sean iguales. Para demostrar este efecto se desarrolla un ejemplo hipotéticode una cobertura de valor razonable en donde se intercambian tasas LIBOR por TIIE. Se comenta que esta distorsión se genera debido a un efecto conocido como “pull to par” especialmente cuando la fecha de valuación no coincide con la fecha de corte de cupón de

Fabrication and Characterization of Anionic Exchange Membranes Based on Chitosan/Poly (Vinyl) Alcohol and Chitosan/Chondroitin Doped with an Amino Cumulene Derivative and Carbon Nanotubes

Anionic exchange membranes mainly based on mixed matrix membranes (MMM) via the crosslinking of either chitosan (CHT) with poly (vinyl alcohol) (CHT : PVA) or CHT with chondroitin sulfate (CHT : CS) in a 50 : 50 wt% were synthesized. The doping of CHT, CHT : PVA and CHT : CS membranes with either the dispersant agent aminocumulene derivative (named AC, coming from the polycondensation of hexamethylenetetramine in an anhydrous acid medium) or with the carbonaceous inch made of multi-walled carbon nanotubes (CNT) dispersed by using AC to perform (AC/CNT) were characterized by the exploration of the topology by scanning electron microscopy, the determination of water uptake, and the analysis of X-ray diffraction spectroscopy together with the measurement of the anionic conductivity by using electrochemical impedance spectroscopy. Results revealed that the modification of the MMMs with the above inorganic carbonaceous fillers, i.e. AC or AC/CNT, improved remarkably the OH– conductivity for all membranes. The modified CHT:CS blend membranes exhibited the highest ionic conductivities of the series. Water uptake increased for the modified CHT and CHT : PVA membranes, whereas a remarkable reduction of water uptake for the CHT : CS based membranes took place. The best membranes in terms of anionic conductivity were correlated with crystallinity and water uptake.The research was carried out at the expense of a grant from the Russian Science Foundation (project No. 15-13-10038). AG acknowledges the Presidential fellowship programme of the Ministry of Education and Science of the Russian Federation. Also LGC and JI are grateful to the Spanish MINECO through the project CTQ2016-76231-C2-2-R

All Static Circularly Symmetric Perfect Fluid Solutions of 2+1 Gravity

Via a straightforward integration of the Einstein equations with cosmological constant, all static circularly symmetric perfect fluid 2+1 solutions are derived. The structural functions of the metric depend on the energy density, which remains in general arbitrary. Spacetimes for fluids fulfilling linear and polytropic state equations are explicitly derived; they describe, among others, stiff matter, monatomic and diatomic ideal gases, nonrelativistic degenerate fermions, incoherent and pure radiation. As a by--product, we demonstrate the uniqueness of the constant energy density perfect fluid within the studied class of metrics. A full similarity of the perfect fluid solutions with constant energy density of the 2+1 and 3+1 gravities is established.Comment: revtex4, 8 page

Resistance Evolution to EPSPS Inhibiting Herbicides in False Barley (Hordeum murinum) Harvested in Southern Spain

A failure of the EPSPS-inhibiting herbicide glyphosate to control several populations of Hordeum murinum subsp. leporinum (or H. murinum) occurred in southern Spain after more than fifteen applications in both crop (olive, orchards, and citrus) and non-crop (dry areas, roadsides and ditches) areas. Eight out of 18 populations studied were resistant (R) to glyphosate with R factors higher than four based on GR50. These populations also had the highest values of LD50 and the lowest levels of shikimic acid accumulation. Two adjuvants tested increased glyphosate efficacy in both susceptible (S) and R populations thanks to better spray foliar retention. Moreover, PS I-, PS II-, and ACCase-inhibiting herbicides, in pre- or post-emergence, proved to be the best chemical alternatives with different sites of action (SoA) to control both S and glyphosate-R populations. This study represents the first report worldwide of glyphosate resistance in H. murinum found in very different crop and non-crop areas from southern Spain. To design chemical strategies to implement integrated weed management programs for glyphosate-R H. murinum, both adjuvants and herbicides with alternative SoA as well as application timings should be considered.This research has also been supported by the Spanish Government, through project AGL2017-83325-C4-2-R (AEI/FEDER/UE), Asociación de Agroquímicos y Medioambiente and project ref PO 4513358935 by Bayer CropScience