Hydrophobic double walled carbon nanotubes interaction with phopholipidic model membranes: 1H-, 2H-, 31P NMR and ESR study

The interactions of carbon nanotubes synthesized by catalytic chemical vapour deposition with phospholipidic bilayers, mimicking biological membranes, have been investigated using solid state 31P- and 2H NMR, 1H- and 31P NMR in liquids and ESR studies. It was found that carbon nanotubes can integrate the bilayer, depending on the overall cohesion of the membrane used. Whereas no direct interaction can be observed in small unilamellar vesicles or directly in the presence of short-chained phospholipids, carbon nanotubes incorporate into the membrane of multibilayers. The result is a significant 2–3 K lowering of the transition temperature in multibilayers of dimyristoyl lecithins, which is more markedly associated with increased fluidity in the most superficial part of the membrane below the transition temperature (292–300 K range). However, no ionophoric property was found on large unilamellar vesicles

IPR, Technology Transfer & Open Science: Challenges and Opportunities

The adoption of Open Science principles is necessary in order to ensure the best use and greatest impact of the investments put into research and innovation in Europe. This “IPR, Technology Transfer & Open Science” workshop was a one day meeting, gathering stakeholders research and innovation ecosystems to ask whether frictions between the IPR laws regulating the freedom of movement of knowledge and the Open Science principles could challenge the progression of Open Science. The workshop aimed to bring together a wide range of expertise to answer the following questions: ● How do you strike the right balance between IPR protection and Open Science? ● How do you achieve the proper balance between the need to freely access data and the need for copyright protections? ● What is the best governance structure and copyright model for the future European Open Science Cloud to be launched in the next 18 months? These three questions were addressed in three separate sessions: Session 1 – The Interplay between Open Science Policy and IPR Session 2 – The Impact of IPR on Open Data Session 3 – The Impact of IPR and Privacy Rules on Research Data Infrastructures andJRC.I.4-Intellectual Property and Technology Transfe

Carbon nanotubes induce inflammation but decrease the production of reactive oxygen species in lung

With the rapid spread of carbon nanotubes (CNTs) applications, the respiratory toxicity of these compounds has attracted the attention of many scientists. Several studies have reported that after lung administration, CNTs could induce granuloma, fibrosis, or inflammation. By comparison with the mechanisms involved with other toxic particles such as asbestos, this effect could be attributed to an increase of oxidative stress. The aim of the present work was to test this hypothesis in vivo. Mice were intranasally instilled with 1.5 mg/kg of double walled carbon nanotubes (DWCNTs). Six, 24, or 48 h after administration, inflammation and localisation of DWCNTs in lungs were microscopically observed. Local oxidative perturbations were investigated using ESR spin trapping experiments, and systemic inflammation was assessed by measuring the plasma concentration of cytokines TNF-α, IL-1α, IL-1β, IL-6, IGF-1, Leptin, G-CSF, and VEGF. Examination of lungs and the elevation of proinflammatory cytokines in the plasma (Leptin and IL-6 at 6 h) confirmed the induction of an inflammatory reaction. This inflammatory reaction was accompanied by a decrease in the local oxidative stress. This effect could be attributed to the scavenger capability of pure CNTs

Cell Patterning with Mucin Biopolymers

The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells, fibroblasts, and myoblasts. These mucin coatings can be patterned with micrometer precision using a microfluidic device, and are stable enough to support myoblast differentiation over seven days. Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Last, we show that a critical surface density of mucins, which is required to achieve cell-repulsion, is efficiently obtained on hydrophobic surfaces, but not on hydrophilic glass surfaces. However, this limitation can be overcome by coating glass with hydrophobic fluorosilane. We conclude that mucin biopolymers are attractive candidates to control cell adhesion on surfaces.European Commission (Marie Curie International Outgoing Fellowship for Career Development, “BIOMUC”)National Institutes of Health (U.S.) (NIH Grant 1R01GM100473)National Science Foundation (U.S.) (award number DMR-819762)National Science Foundation (U.S.) (NSF Grant OCE-0744641-CAREER)National Science Foundation (U.S.) (Award DMR-0819762)Samsung Scholarship FoundationMassachusetts Institute of Technology (Startup funds)Massachusetts Institute of Technology (Junior Faculty award

Biological effects of double-walled carbon nanotubes on the innate immune system: An in vitro study on THP-1 human monocytes

DWCNTs have numerous industrial and biomedical applications and several studies reported that they could act as immunomodulator systems. The immune system is the first line of defence of the human body when exposed to particulate matter. In order to investigate DWCNTs’ role on innate immunity, we used THP-1 monocytic cells for the purpose of this study. We showed that DWCNTs were not cytotoxic until 6 h, 24 h, 48 h and 72 h of incubation with THP-1 monocytic cells (concentrations tested from 10 to 50 μg/mL). From 6 h to 72 h of incubation of THP-1 cells with DWCNTs, we measured a significant increase of the baseline cell index using xCELLigence® technology showing cell adhesion. After 24 h of exposure, DWCNTs agglomerates were localized in THP-1 monocyte cytoplasm and cell adhesion was observed simultaneously with a significant increase in the expression of CD11b and CD14 cell surface proteins. Pro-inflammatory cytokine secretion (IL-1β, IL-6, IL-8, TNF-α and IL-10) was also measured in supernatants after 6 h or 24 h of exposure to DWCNTs. This pro-inflammatory response was increased in THP-1 monocytic cells pre-treated with LPS. Altogether, our data indicate that DWCNTs induce an increased pro-inflammatory response of THP-1 monocytes and seem to modulate cell surface protein expression confirming that DWCNTs could act as stimulators of innate immunity

An experimental testbed for NEAT to demonstrate micro-pixel accuracy

NEAT is an astrometric mission proposed to ESA with the objectives of detecting Earth-like exoplanets in the habitable zone of nearby solar-type stars. In NEAT, one fundamental aspect is the capability to measure stellar centroids at the precision of 5e-6 pixel. Current state-of-the-art methods for centroid estimation have reached a precision of about 4e-5 pixel at Nyquist sampling. Simulations showed that a precision of 2 micro-pixels can be reached, if intra and inter pixel quantum efficiency variations are calibrated and corrected for by a metrology system. The European part of the NEAT consortium is designing and building a testbed in vacuum in order to achieve 5e-6 pixel precision for the centroid estimation. The goal is to provide a proof of concept for the precision requirement of the NEAT spacecraft. In this paper we give the basic relations and trade-offs that come into play for the design of a centroid testbed and its metrology system. We detail the different conditions necessary to reach the targeted precision, present the characteristics of our current design and describe the present status of the demonstration.Comment: SPIE proceeding

Evolution comparée de la consommation de médicaments dans les 5 pays européens entre 2000 et 2004 : analyse de 7 classes pharmaco-thérapeutiques

The drug consumption level in France is often considered as being one of the most important in Europe. This study aims at confirming this assertion over the period 2000-2004 by comparing drug consumption in Germany, Spain, France, Italy and the United-Kingdom. The results show that in 2004 drug consumption in France was no more the biggest for all the seven studied classes. Moreover, the consumption levels of the five countries have converged between 2000 and 2004, as well as the consumption structures.Defined Daily Dose (DDD); Drug Consumption; European Comparative Analysis

Myoconductive and osteoinductive free-standing polysaccharide membranes

Free-standing (FS) membranes have increasing applications in the biomedical field as drug delivery systems for wound healing and tissue engineering. Here, we studied the potential of free-standing membranes made by the layer-by-layer assembly of chitosan and alginate to be used as a simple biomimetic system of the periosteum. The design of a periosteum-like membrane implies the elaboration of a thick membrane suitable for both muscle and bone formation. Our aim was to produce well-defined ∼50 μm thick polysaccharide membranes that could be easily manipulated, were mechanically resistant, and would enable both myogenesis and osteogenesis in vitro and in vivo. The membranes were chemically crosslinked to improve their mechanical properties. Crosslinking chemistry was followed via Fourier transform infrared spectroscopy and the mechanical properties of the membranes were assessed using dynamic mechanical analysis. The loading and release of the potent osteoinductive growth factor bone morphogenetic protein 2 (BMP-2) inside and outside of the FS membrane was followed by fluorescence spectroscopy in a physiological buffer over 1 month. The myogenic and osteogenic potentials of the membranes in vitro were assessed using BMP-2-responsive skeletal myoblasts. Finally, their osteoinductive properties in vivo were studied in a preliminary experiment using a mouse ectopic model. Our results showed that the more crosslinked FS membranes enabled a more efficient myoblast differentiation in myotubes. In addition, we showed that a tunable amount of BMP-2 can be loaded into and subsequently released from the membranes, depending on the crosslinking degree and the initial BMP-2 concentration in solution. Only the more crosslinked membranes were found to be osteoinductive in vivo. These polysaccharide-based membranes have strong potential as a periosteum-mimetic scaffold for bone tissue regeneration.This work was financially supported by the Foundation for Science and Technology (FCT) through the scholarship SFRH/BPD/96797/2013, Fundo Social Europeu (FSE), and Programa Diferencial de Potencial Human (POPH) granted to Sofia G. Caridade. C.M. is indebted to the Association Francaise contre les Myopathies for financial support via a post-doctoral fellowship (AFM project 16673). J.A. acknowledges the Whitaker International Fellows and Scholars Program for support via a post-doctoral fellowship. This work was supported by the European Commission (FP7 program) via a European Research Council starting grant (BIOMIM, GA 259370 to C.P.) and by the AFM (grant Microtiss, 16530). We thank Isabelle Paintrand for her technical help with the confocal apparatus

First experimental results of very high accuracy centroiding measurements for the neat astrometric mission

NEAT is an astrometric mission proposed to ESA with the objectives of detecting Earth-like exoplanets in the habitable zone of nearby solar-type stars. NEAT requires the capability to measure stellar centroids at the precision of 5e-6 pixel. Current state-of-the-art methods for centroid estimation have reached a precision of about 2e-5 pixel at two times Nyquist sampling, this was shown at the JPL by the VESTA experiment. A metrology system was used to calibrate intra and inter pixel quantum efficiency variations in order to correct pixelation errors. The European part of the NEAT consortium is building a testbed in vacuum in order to achieve 5e-6 pixel precision for the centroid estimation. The goal is to provide a proof of concept for the precision requirement of the NEAT spacecraft. In this paper we present the metrology and the pseudo stellar sources sub-systems, we present a performance model and an error budget of the experiment and we report the present status of the demonstration. Finally we also present our first results: the experiment had its first light in July 2013 and a first set of data was taken in air. The analysis of this first set of data showed that we can already measure the pixel positions with an accuracy of about 1e-4 pixel.Comment: SPIE conference proceeding

A detector interferometric calibration experiment for high precision astrometry

Context: Exoplanet science has made staggering progress in the last two decades, due to the relentless exploration of new detection methods and refinement of existing ones. Yet astrometry offers a unique and untapped potential of discovery of habitable-zone low-mass planets around all the solar-like stars of the solar neighborhood. To fulfill this goal, astrometry must be paired with high precision calibration of the detector. Aims: We present a way to calibrate a detector for high accuracy astrometry. An experimental testbed combining an astrometric simulator and an interferometric calibration system is used to validate both the hardware needed for the calibration and the signal processing methods. The objective is an accuracy of 5e-6 pixel on the location of a Nyquist sampled polychromatic point spread function. Methods: The interferometric calibration system produced modulated Young fringes on the detector. The Young fringes were parametrized as products of time and space dependent functions, based on various pixel parameters. The minimization of func- tion parameters was done iteratively, until convergence was obtained, revealing the pixel information needed for the calibration of astrometric measurements. Results: The calibration system yielded the pixel positions to an accuracy estimated at 4e-4 pixel. After including the pixel position information, an astrometric accuracy of 6e-5 pixel was obtained, for a PSF motion over more than five pixels. In the static mode (small jitter motion of less than 1e-3 pixel), a photon noise limited precision of 3e-5 pixel was reached