124 research outputs found
Revisiting tectonic corrections applied to Pleistocene sea-level highstands
Tectonic displacement contaminates estimates of peak eustatic sea level (and, equivalently, minimum continental ice volumes) determined from the elevation of Quaternary interglacial highstand markers. For sites at which a stratigraphic or geomorphic marker of peak Marine Isotope Stage (MIS) 5e sea level exists, the standard approach for estimating local tectonic uplift (or subsidence) rates takes the difference between the elevation of the local highstand marker and a reference MIS 5e eustatic value, commonly chosen as +6 m, and divides by the age of the marker. The resulting rate is then applied to correct the elevation of all other local observed sea-level markers for tectonic displacement, including peak highstands of different ages (e.g., MIS 5a, MIS 5c and MIS 11), under the assumption that the tectonic rate remained constant over those periods. This approach introduces two potentially significant errors. First, the peak eustatic value adopted for MIS 5e in most previous studies (i.e., +6 m) is likely incorrect. Second, local peak sea level during MIS 5e is characterized by significant departures from eustasy due to glacial isostatic adjustment in response to both successive glacial–interglacial cycles and excess polar ice-sheet melt relative to present day values. We use numerical models of glacial isostatic adjustment that incorporate both of these effects to quantify the plausible range of the combined error and show that, even at sites far from melting ice sheets, local peak sea level during MIS 5e may depart from eustasy by 2–4 m, or more. We also demonstrate that the associated error in the estimated tectonic rates can significantly alter previous estimates of peak eustatic sea level during Quaternary highstands, notably those associated with earlier interglacials (e.g., MIS 11)
Biochemical Characterization of a Filtered Synaptoneurosome Preparation from Guinea Pig Cerebral Cortex: Cyclic Adenosine 3’:5’-Monophosphate-generating Systems, Receptors, and Enzymes
A particulate preparation was obtained by low speed centrifugation of guinea pig cerebral cortical homogenates prepared with a Krebs-Henseleit buffer. Light microscopic examination, using a reflected light differential interference contrast system, reveals the presence of intact neurons, axonal fragments, glial cells, and erythrocytes along with an abundance of small spherical entities (diameter about 1.1 μm) and snowman-shaped entities (diameter of larger sphere about 1.1 μm, diameter of attached smaller sphere about 0.6 μm). Many unattached smaller spherical entities are also present (diameter about 0.6 μm). Pressure filtration through 5 or l0-μm Millipore filters, followed by low speed centrifugation and resuspension, removes most of the larger entities to afford a suspension composed mainly of the small spherical and snowman-shaped entities. Electron microscopic examination reveals the presence of many synaptosomes with attached resealed postsynaptic entities. It is proposed that these correspond to the snowman-shaped entities to be termed synaptoneurosomes. Accumulations of cyclic AMP elicited by 2-chloroadenosine and histamine, and by combinations of 2-chloroadenosine, histamine, norepinephrine, and forskolin, are lower in filtered than in unfiltered preparations, whereas accumulations elicited by forskolin are unchanged. Levels of adenylate cyclase are reduced by filtration, whereas levels of phosphodiesterase are unchanged. Filtration reduces levels of markers for whole cells and endothelial cells, whereas neuronal markers such as acetylcholinesterase activity and norepinephrine uptake are increased. Levels of S-100 protein, a marker for glial cells, are not significantly decreased. There is no apparent change in the density of many receptors or ion channels. Levels of A1-adenosine and H1-histamine receptors are increased, whereas levels of so-called peripheral benzodiazepine-binding sites are decreased
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Phosphorus sources for phosphatic Cambrian carbonates
The fossilization of organic remains and shell material by calcium phosphate minerals provides an illuminating, but time-bounded, window into Ediacaran–Cambrian animal evolution. For reasons that remain unknown, phosphatic fossil preservation declined significantly through Cambrian Series 2. Here, we investigate the phosphorus (P) sources for phosphatic Cambrian carbonates, presenting sedimentological, petrographic, and geochemical data from the Cambrian Series 2–3 Thorntonia Limestone, Australia, some of the youngest Cambrian strata to display exceptional phosphatic preservation of small shelly fossils. We find that within Thorntonia sediments, phosphate was remobilized by organic decay and bacterial iron reduction, with subsequent reprecipitation largely as apatite within the interiors of small shelly fossils. We discuss the merits of bioclastic-derived, organic matter–bound, or iron-bound P as potential sources to these strata. Petrographic observations suggest that the dissolution of phosphatic skeletal material did not provide the P for fossil preservation. In contrast, high organic carbon contents imply significant organic fluxes of P to Thorntonia sediments. Sedimentology and iron-speciation data indicate that phosphorus enrichment occurred during times of expanded anoxic, ferruginous conditions in subsurface water masses, suggesting that phosphorus adsorption to iron minerals precipitating from the water column provided a second significant P source to Thorntonia sediments. Simple stoichiometric models suggest that, by themselves, neither organic carbon burial nor an iron shuttle can account for the observed phosphorus enrichment. Thus, we infer that both processes were necessary for the observed phosphorus enrichment and subsequent fossil preservation in the Thorntonia Limestone.Earth and Planetary Science
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Snowball Earth climate dynamics and Cryogenian geology-geobiology
Geological evidence indicates that grounded ice sheets reached sea level at all latitudes during two long-lived Cryogenian (58 and ≥5 My) glaciations. Combined uranium-lead and rhenium-osmium dating suggests that the older (Sturtian) glacial onset and both terminations were globally synchronous. Geochemical data imply that CO2 was 102 PAL (present atmospheric level) at the younger termination, consistent with a global ice cover. Sturtian glaciation followed breakup of a tropical supercontinent, and its onset coincided with the equatorial emplacement of a large igneous province. Modeling shows that the small thermal inertia of a globally frozen surface reverses the annual mean tropical atmospheric circulation, producing an equatorial desert and net snow and frost accumulation elsewhere. Oceanic ice thickens, forming a sea glacier that flows gravitationally toward the equator, sustained by the hydrologic cycle and by basal freezing and melting. Tropical ice sheets flow faster as CO2 rises but lose mass and become sensitive to orbital changes. Equatorial dust accumulation engenders supraglacial oligotrophic meltwater ecosystems, favorable for cyanobacteria and certain eukaryotes. Meltwater flushing through cracks enables organic burial and submarine deposition of airborne volcanic ash. The subglacial ocean is turbulent and well mixed, in response to geothermal heating and heat loss through the ice cover, increasing with latitude. Terminal carbonate deposits, unique to Cryogenian glaciations, are products of intense weathering and ocean stratification. Whole-ocean warming and collapsing peripheral bulges allow marine coastal flooding to continue long after ice-sheet disappearance. The evolutionary legacy of Snowball Earth is perceptible in fossils and living organisms
Geobiology of a lower Cambrian carbonate platform, Pedroche Formation, Ossa Morena Zone, Spain
The Cambrian Pedroche Formation comprises a mixed siliciclastic-carbonate succession recording subtidal deposition on a marine platform. Carbonate carbon isotope chemostratigraphy confirms previous biostratigraphic assignment of the Pedroche Formation to the Atdabanian regional stage of Siberia, correlative to Cambrian Series 2. At the outcrop scale, thrombolitic facies comprise ~. 60% of carbonate-normalized stratigraphy and coated-grains another ~. 10%. Petrographic point counts reveal that skeletons contribute at most 20% to thrombolitic inter-reef and reef-flank lithologies; on average, archaeocyath clasts make up 68% of skeletal materials. In contrast, petrographic point counts show that skeletons comprise a negligible volume of biohermal and biostromal thrombolite, associated nodular carbonate facies, and ooid, oncoid and peloid grainstone facies. As such, archaeocyathan reefal bioconstructions represent a specific and limited locus of skeletal carbonate production and deposition. Consistent with data from coeval, globally dispersed lower Cambrian successions, our analysis of the Pedroche Formation supports the view that lower Cambrian carbonates have more in common with earlier, Neoproterozoic deposits than with younger carbonates dominated by skeletal production and accumulation. © 2013 Elsevier B.V.Jessica R. Creveling, David Fernández-Remolar, Marta Rodríguez-Martínez, Silvia Menéndez, Kristin D. Bergmann, Benjamin C. Gill, John Abelson, Ricardo Amils, Bethany L. Ehlmann, Diego C. García-Bellido, John P. Grotzinger, Christian Hallmann, Kathryn M. Stack, Andrew H. Knol
Stimulated Formation of Adenosine 3',5'-Cyclic Phosphate in Cerebral Cortex: Synergism between Electrical Activity and Biogenic Amines
Catechol-O-methyltransferase: Factors Relating to the Carcinogenic Potential of Catecholestrogens
An Enzyme Cascade for Selective Modification of Tyrosine Residues in Structurally Diverse Peptides and Proteins.
Bioorthogonal chemistry enables a
specific moiety in a complex
biomolecule to be selectively modified in the presence of many reactive
functional groups and other cellular entities. Such selectivity has
become indispensable in biology, enabling biomolecules to be derivatized,
conjugated, labeled, or immobilized for imaging, biochemical assays,
or therapeutic applications. Methyltransferase enzymes (MTase) that
accept analogues of the cofactor <i>S</i>-adenosyl methionine
have been widely deployed for alkyl-diversification and bioorthogonal
labeling. However, MTases typically possess tight substrate specificity.
Here we introduce a more flexible methodology for selective derivatization
of phenolic moieties in complex biomolecules. Our approach relies
on the tandem enzymatic reaction of a fungal tyrosinase and the mammalian
catechol-<i>O</i>-methyltransferase (COMT), which can effect
the sequential hydroxylation of the phenolic group to give an intermediate
catechol moiety that is subsequently <i>O</i>-alkylated.
When used in this combination, the alkoxylation is highly selective
for tyrosine residues in peptides and proteins, yet remarkably tolerant
to changes in the peptide sequence. Tyrosinase–COMT are shown
to provide highly versatile and regioselective modification of a diverse
range of substrates including peptide antitumor agents, hormones,
cyclic peptide antibiotics, and model proteins
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