16 research outputs found
Interaction between the calcium and adenylate cyclase messenger systems in dispersed chief cells from guinea pig stomach. Possible cellular mechanism for potentiation of pepsinogen secretion.
No full textDNA Metabolism During Infection of Anacystis Nidulans By Cyanophage AS-1. VII. UV-Induced Alterations of the AS-1/a. Nidulans Lytic Cycle
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The Groove between the α- and β-Subunits of Hormones with Lutropin (LH) Activity Appears to Contact the LH Receptor, and Its Conformation Is Changed during Hormone Binding
No full textFunctional homodimeric glycoprotein hormones: implications for hormone action and evolution
No full textIdentification and selective destruction of shared epitopes in human chorionic gonadotropin beta subunit
No full textEffect of modification of all loop regions in the α- and β-subunits of human choriogonadotropin on its signal transduction activity
No full textMapping the receptor binding regions of human chorionic gonadotropin (hCG) using disulfide peptides of its β-subunit: possible involvement of the disulfide bonds Cys 9
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A Defined Epitope on the Human Choriogonadotropin α-Subunit Interacts with the Second Extracellular Loop of the Transmembrane Domain of the Lutropidchoriogonadotropin Receptor
No full textInternational audienceThe monoclonal antibody, HT13 recognizes human choriogonadotropin (CG) bound to the extracellular domain of its receptor, but not to the full-length receptor. The HT13 epitope is located in the regions of residues 15-17 and 73-75 of the human CG alpha-subunit. Only one synthetic peptide, lutropin (LH)/CG-receptor-(481-497)-peptide (EL2 peptide), which spans the second putative extracellular loop of the LH/CG-receptor endodomain, prevents recognition of human CG by HT13 mAb. EL2 peptide decreases hormone-induced cAMP production, but not high-affinity binding. An anti-EL2 serum also displays the capacity to inhibit human CG-stimulated cAMP production. These results suggest that the second extracellular loop of the receptor is in contact with the HT13 epitope of human CG alpha-subunit and is involved in signal transduction. A relative orientation of the hormone versus the endodomain is proposed
