Growth and Reproduction of Southern Flounder (\u3ci\u3eParalichthys lethostigma\u3c/i\u3e) in the North-Central Gulf of Mexico

Southern Flounder Paralichthys lethostigma is the most commonly harvested flatfish in the north-central Gulf of Mexico (GOM) and supports a major inshore recreational fishery, yet knowledge of the species’ life history is greatly limited. The objective of this research was to describe the growth and reproduction of Southern Flounder in the Mississippi stock. Fish were collected during September 2014 to March 2016 using primarily recreational fishing techniques. Otoliths (n = 313) were sectioned to estimate age, and multiple length-at-age models were fit to total length (TL, mm) and age estimate (y) data. Gonadal tissue samples (n = 221) were preserved for histological analysis and used to classify reproductive phases. Length-at-age model fit was evaluated using Akaike information criteria, revealing that the three-parameter von Bertalanffy growth function best described the female-specific data (L∞ = 509 mm, k = 0.70 y-1, t0= -0.46 y). By fitting a logistic model to binomial maturity data, the mean length-at-50% maturity was estimated as 303 mm TL and mean age-at-50% maturity was estimated as one year for females. Histological indicators and gonadosomatic index (GSI) data were used to estimate that the spawning season lasts from November to January, and to classify Southern Flounder as batch spawners. These results will inform future stock assessments and management decisions for the GOM Flounder fishery

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Applying the ARRIVE Guidelines to an In Vivo Database.

The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were developed to address the lack of reproducibility in biomedical animal studies and improve the communication of research findings. While intended to guide the preparation of peer-reviewed manuscripts, the principles of transparent reporting are also fundamental for in vivo databases. Here, we describe the benefits and challenges of applying the guidelines for the International Mouse Phenotyping Consortium (IMPC), whose goal is to produce and phenotype 20,000 knockout mouse strains in a reproducible manner across ten research centres. In addition to ensuring the transparency and reproducibility of the IMPC, the solutions to the challenges of applying the ARRIVE guidelines in the context of IMPC will provide a resource to help guide similar initiatives in the future

Applying the ARRIVE Guidelines to an In Vivo Database.

The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were developed to address the lack of reproducibility in biomedical animal studies and improve the communication of research findings. While intended to guide the preparation of peer-reviewed manuscripts, the principles of transparent reporting are also fundamental for in vivo databases. Here, we describe the benefits and challenges of applying the guidelines for the International Mouse Phenotyping Consortium (IMPC), whose goal is to produce and phenotype 20,000 knockout mouse strains in a reproducible manner across ten research centres. In addition to ensuring the transparency and reproducibility of the IMPC, the solutions to the challenges of applying the ARRIVE guidelines in the context of IMPC will provide a resource to help guide similar initiatives in the future

A summary of the areas encompassed by the ARRIVE guidelines.

<p>The ARRIVE guidelines are organised into twenty sections, which cover all areas of a typical research manuscript. <i>Image credit</i>: NC3Rs: <a href="https://www.nc3rs.org.uk/arrive-guidelines" target="_blank">https://www.nc3rs.org.uk/arrive-guidelines</a>.</p

Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Although next-generation sequencing has revolutionized the ability to associate variants with human diseases, diagnostic rates and development of new therapies are still limited by a lack of knowledge of the functions and pathobiological mechanisms of most genes. To address this challenge, the International Mouse Phenotyping Consortium is creating a genome- and phenome-wide catalog of gene function by characterizing new knockout-mouse strains across diverse biological systems through a broad set of standardized phenotyping tests. All mice will be readily available to the biomedical community. Analyzing the first 3,328 genes identified models for 360 diseases, including the first models, to our knowledge, for type C Bernard-Soulier, Bardet-Biedl-5 and Gordon Holmes syndromes. 90% of our phenotype annotations were novel, providing functional evidence for 1,092 genes and candidates in genetically uncharacterized diseases including arrhythmogenic right ventricular dysplasia 3. Finally, we describe our role in variant functional validation with The 100,000 Genomes Project and others