Aislamiento y caracterización de proteínas implicadas en la meiosis de arroz

Comunicaciones a congreso

Unravelling the proteomic profile of rice meiocytes during early meiosis

Transfer of genetic traits from wild or related species into cultivated rice is nowadays an important aim in rice breeding. Breeders use genetic crosses to introduce desirable genes from exotic germplasms into cultivated rice varieties. However, in many hybrids there is only a low level of pairing (if existing) and recombination at early meiosis between cultivated rice and wild relative chromosomes. With the objective of getting deeper into the knowledge of the proteins involved in early meiosis, when chromosomes associate correctly in pairs and recombine, the proteome of isolated rice meiocytes has been characterized by nLC-MS/MS at every stage of early meiosis (prophase I). Up to 1316 different proteins have been identified in rice isolated meiocytes in early meiosis, being 422 exclusively identified in early prophase I (leptotene, zygotene, or pachytene). The classification of proteins in functional groups showed that 167 were related to chromatin structure and remodeling, nucleic acid binding, cell-cycle regulation, and cytoskeleton. Moreover, the putative roles of 16 proteins which have not been previously associated to meiosis or were not identified in rice before, are also discussed namely: seven proteins involved in chromosome structure and remodeling, five regulatory proteins [such as SKP1 (OSK), a putative CDK2 like effector], a protein with RNA recognition motifs, a neddylation-related protein, and two microtubule-related proteins. Revealing the proteins involved in early meiotic processes could provide a valuable tool kit to manipulate chromosome associations during meiosis in rice breeding programs. The data have been deposited to the ProteomeXchange with the PXD001058 identifier.This research was supported by grants ERC-StG-243118 from the FP7 and The European Regional Development Fund (FEDER) from the European Union.Peer Reviewe

Employability skills: a system of recognition at Universitat Jaume I

Proceedings 10th International Conference of Education, Research and Innovation (ICERI2017)Following CEDEFOP [1] the employability is “the combination of factors which enable individuals to progress towards or get into employment, to stay in employment and to progress during their career.” Also, theories of employability have become increasingly complex and multi-dimensional in the last decades. [2]. Employability depends on the knowledge, skills and attitudes, how the graduate use those assets, and how the graduates present them to employers. Moreover, there is an amount of work developed by Careers Services and faculties enhancing employabiity of our students through diferent initiatives and programmes (placements, workshops, career guidance, traning for job search, and so forth). Also University Jaume I has developed an educational model, in which their 10 principles we find “promotion of employability and the smart entrepreneurial spirit”. However, from the vicerectorate for Students, Employment and Educational Innovation we want to go a step forward to enhance employability of our graduates launching a formal document that will be an edge in their curriculum. The main aim of this system is to show a potential employer evidences of high impact employability soft and hard competences, achieved by graduates and undergraduates during their studies. This competences have been developed by the students through active participation in both extracurricular and curricular training, increasing of sensitivity and experience (skills acquired by practice) activities organised both by the University or other organisations, and recognised by an expert committee. The aim of this communication is to explain the features of the system: background, requeriments for each activity, administrative procedure, and results. Also we will comment on progress and future challenges of the system

A pleiotropic variant in DNAJB4 is associated with multiple myeloma risk

This study was partially supported by intramural funds of University of Pisa and DKFZ. Open Access Funding provided by Universita degli Studi di Pisa within the CRUI-CARE Agreement.Pleiotropy, which consists of a single gene or allelic variant affecting multiple unrelated traits, is common across cancers, with evidence for genome-wide significant loci shared across cancer and noncancer traits. This feature is particularly relevant in multiple myeloma (MM) because several susceptibility loci that have been identified to date are pleiotropic. Therefore, the aim of this study was to identify novel pleiotropic variants involved in MM risk using 28 684 independent single nucleotide polymorphisms (SNPs) from GWAS Catalog that reached a significant association (P < 5 x 10(-8)) with their respective trait. The selected SNPs were analyzed in 2434 MM cases and 3446 controls from the International Lymphoma Epidemiology Consortium (InterLymph). The 10 SNPs showing the strongest associations with MM risk in InterLymph were selected for replication in an independent set of 1955 MM cases and 1549 controls from the International Multiple Myeloma rESEarch (IMMEnSE) consortium and 418 MM cases and 147 282 controls from the FinnGen project. The combined analysis of the three studies identified an association between DNAJB4-rs34517439-A and an increased risk of developing MM (OR = 1.22, 95%CI 1.13-1.32, P = 4.81 x 10(-7)). rs34517439-A is associated with a modified expression of the FUBP1 gene, which encodes a multifunctional DNA and RNA-binding protein that it was observed to influence the regulation of various genes involved in cell cycle regulation, among which various oncogenes and oncosuppressors. In conclusion, with a pleiotropic scan approach we identified DNAJB4-rs34517439 as a potentially novel MM risk locus.University of PisaDKFZUniversita degli Studi di Pisa within the CRUI-CARE Agreemen

La inactividad de la administración en el ejercicio de la potestad de restablecimiento del orden urbanístico infringido por las edificaciones ilegales

La hipotesis de la tesis se basa en la indolencia de la administracion publica y su falta de aplicacion en el cumplimiento del deber de proteccion de la legalidad urbanistica frente a una edificacion ilegal. la proposicion que se mantiene es que el establecimiento de configuraciones singularizadas y de diferentes plazos para ejercitar la accion de restauracion de la legalidad urbanistica, por los poderes legislativos de las ccaa, contraviene el principio constitucional de igualdad en el ejercicio del derecho de propiedad privada del suelo y que la falta de actuacion del poder de proteccion de la legalidad ya no puede constituir, desde la entrada en vigor de la ley del suelo de 2007, un hecho juridico en virtud del cual el propietario del suelo pudiera patrimonializar el valor urbanistico de una edificacion ilegal. porque solo los criterios legales de valoracion determinan el alcance patrimonial de las facultades que pueden llegar a formar parte del contenido del derecho de propiedad del suelo. a traves del trabajo se ha podido deducir que, pese al reconocimiento legal del concepto estatutario del derecho de propiedad del suelo, se ha seguido manteniendo una concepcion civilista del derecho, que, unida a la falta de control de la inactividad administrativa, ha derivado, cuando la administracion ya no podia ejercitar la accion de restauracion de esa legalidad por el transcurso de los plazos, en la equiparacion de la edificacion ilegal a las edificaciones legales, o en su asimilacion a las edificaciones en situacion de fuera de ordenacion. los diferentes plazos de caducidad o de prescripcion de la citada accion han creado importantes disfunciones del principio establecido en el articulo 149.1.1 ce, por lo que los objetivos de la investigacion ha sido acreditar la necesidad de una regulacion igualitaria para todo el estado de la actuacion de la administracion frente a la ilegalidad edificatoria y que el ejercicio de la potestad de restablecimiento de la legalidad no se sujete a plazo

Procesos por delitos fiscales: problemática actual

Tesis original leida en: Universidad Autónoma de Madrid, Facultad de Derecho, Departamento de Derecho Privado, 199

Functional Capacity of Tai Chi-Practicing Elderly People

[EN] Research shows that ageing is modifiable or modulable, attending to external modifications and lifestyle factors: physical activity has a unique contribution to functional health and energy balance. Extensive research shows Tai Chi (TC) produced a major physical condition. To determine the impact of lifestyle on functional capacity, comparing the impact of continued long-life practice. 113 individuals (±71.53 years old): (a) PTC (n = 27); senior competitors, life-long training; (b) TC (n = 27); ±4 years; (c) Keep-Fit (KF n = 36); ±4 years; and the control group (d) sedentary individuals (SI n = 23). Five tests from the Senior Fitness Test (SFT) were used to assess the physical condition. The TC group showed significantly better results than the KF group: 30-s chair stand (23.22 ± 3.08 * rep vs. 17.17 ± 2.96 rep), chair sit-and-reach (2.19 ± 4.85 * cm vs. −1.93 ± 5.46 cm) and back scratch (1.02 ± 4.46 * cm vs. −2.43 ± 5.78 cm). The TCP group showed better results than the TC group: 30-s chair stand (27.70 ± 4.98 * rep vs. 23.22 ± 3.08 rep), 30-s arm curl (30.22 ± 4.36 * rep vs. 23.48 ± 3.42 rep), chair sit-and-reach (13.07 ± 4.00 * cm vs. 2.19 ± 4.85 cm) and back scratch (5.48 ± 3.51 * cm vs. 1.02 ± 4.46 cm). Among the different activities analysed, TC showed better results in SFT tests; in particular considering the long-life training of this martial art.S

Selection for Unequal Densities of σ(70) Promoter-Like Signals in Different Regions of Large Bacterial Genomes

The evolutionary processes operating in the DNA regions that participate in the regulation of gene expression are poorly understood. In Escherichia coli, we have established a sequence pattern that distinguishes regulatory from nonregulatory regions. The density of promoter-like sequences, that could be recognizable by RNA polymerase and may function as potential promoters, is high within regulatory regions, in contrast to coding regions and regions located between convergently transcribed genes. Moreover, functional promoter sites identified experimentally are often found in the subregions of highest density of promoter-like signals, even when individual sites with higher binding affinity for RNA polymerase exist elsewhere within the regulatory region. In order to see the generality of this pattern, we have analyzed 43 additional genomes belonging to most established bacterial phyla. Differential densities between regulatory and nonregulatory regions are detectable in most of the analyzed genomes, with the exception of those that have evolved toward extreme genome reduction. Thus, presence of this pattern follows that of genes and other genomic features that require weak selection to be effective in order to persist. On this basis, we suggest that the loss of differential densities in the reduced genomes of host-restricted pathogens and symbionts is an outcome of the process of genome degradation resulting from the decreased efficiency of purifying selection in highly structured small populations. This implies that the differential distribution of promoter-like signals between regulatory and nonregulatory regions detected in large bacterial genomes confers a significant, although small, fitness advantage. This study paves the way for further identification of the specific types of selective constraints that affect the organization of regulatory regions and the overall distribution of promoter-like signals through more detailed comparative analyses among closely related bacterial genomes

Trabajo fin de máster

Memoria sobre dos actividades realizadas durante el Máster de Profesorado