Frontier Fields: High-Redshift Predictions and Early Results

The Frontier Fields program is obtaining deep Hubble and Spitzer Space Telescope images of new "blank" fields and nearby fields gravitationally lensed by massive galaxy clusters. The Hubble images of the lensed fields are revealing nJy sources (AB mag > 31), the faintest galaxies yet observed. In this paper, we present high-redshift (z > 6) number count predictions for the full program and candidates in three of the first Hubble Frontier Fields images. The full program will transform our understanding of galaxy evolution in the first 600 million years (z > 9). Where previous programs yielded perhaps a dozen z > 9 candidates, the Frontier Fields may yield ~70 (~6 per field). We base this estimate on an extrapolation of luminosity functions observed between 4 < z < 8 and gravitational lensing models submitted by the community. However, in the first two deep infrared Hubble images obtained to date, we find z ~ 8 candidates but no strong candidates at z > 9. This might suggest a deficit of faint z > 9 galaxies as also reported in the Ultra Deep Field (even while excesses of brighter z > 9 galaxies were reported in shallower fields). At these redshifts, cosmic variance (field-to-field variation) is expected to be significant (greater than +/-50%) and include clustering of early galaxies formed in overdensities. The full Frontier Fields program will significantly mitigate this uncertainty by observing six independent sightlines each with a lensing cluster and nearby blank field.Comment: Submitted for publication in the Astrophysical Journal. 15 pages, 17 figure

CLASH: Discovery of a Bright z~6.2 Dwarf Galaxy Quadruply Lensed by MACS J0329.6-0211

We report the discovery of a z_{phot}=6.18^{+0.05}_{-0.07} (95% confidence level) dwarf galaxy, lensed into four images by the galaxy cluster MACS J0329.6-0211 (z_{l}=0.45). The galaxy is observed as a high-redshift dropout in HST/ACS/WFC3 CLASH and Spitzer/IRAC imaging. Its redshift is securely determined due to a clear detection of the Lyman-break in the 18-band photometry, making this galaxy one of the highest-redshift multiply-lensed objects known to date with an observed magnitude of F125W=24.00\pm0.04 AB mag for its highest-magnified image. We also present the first strong-lensing analysis of this cluster uncovering 15 additional multiply-imaged candidates of five lower-redshift sources spanning the range z_{s}~2-4. The mass model independently supports the high photometric redshift and reveals magnifications of 11.6^{+8.9}_{-4.1}, 17.6^{+6.2}_{-3.9}, 3.9^{+3.0}_{-1.7}, and 3.7^{+1.3}_{-0.2}, respectively, for the four images of the high-redshift galaxy. With this we construct a source image with a physical resolution of ~200 pc when the universe was ~0.9 Gyr old, where the z~6.2 galaxy occupies a source-plane area of approximately 2.2 kpc^{2}. Modeling the observed spectral energy distribution using population synthesis models, we find a demagnified stellar mass of ~10^{9} {M}_{sun}, subsolar metallicity (Z/Z_{sun}~0.5), low dust content (A_{V}~0.1 mag), a demagnified star formation rate (SFR) of ~3.2 {M}_{sun} yr^{-1}, and a specific SFR of ~3.4 Gyr^{-1}, all consistent with the properties of local dwarf galaxies.Comment: 6 pages, 1 table, 4 figures, accepted for publication in ApJ Letters; v3: minor changes including shortened text and figure

SeeGH – A software tool for visualization of whole genome array comparative genomic hybridization data

BACKGROUND: Array comparative genomic hybridization (CGH) is a technique which detects copy number differences in DNA segments. Complete sequencing of the human genome and the development of an array representing a tiling set of tens of thousands of DNA segments spanning the entire human genome has made high resolution copy number analysis throughout the genome possible. Since array CGH provides signal ratio for each DNA segment, visualization would require the reassembly of individual data points into chromosome profiles. RESULTS: We have developed a visualization tool for displaying whole genome array CGH data in the context of chromosomal location. SeeGH is an application that translates spot signal ratio data from array CGH experiments to displays of high resolution chromosome profiles. Data is imported from a simple tab delimited text file obtained from standard microarray image analysis software. SeeGH processes the signal ratio data and graphically displays it in a conventional CGH karyotype diagram with the added features of magnification and DNA segment annotation. In this process, SeeGH imports the data into a database, calculates the average ratio and standard deviation for each replicate spot, and links them to chromosome regions for graphical display. Once the data is displayed, users have the option of hiding or flagging DNA segments based on user defined criteria, and retrieve annotation information such as clone name, NCBI sequence accession number, ratio, base pair position on the chromosome, and standard deviation. CONCLUSIONS: SeeGH represents a novel software tool used to view and analyze array CGH data. The software gives users the ability to view the data in an overall genomic view as well as magnify specific chromosomal regions facilitating the precise localization of genetic alterations. SeeGH is easily installed and runs on Microsoft Windows 2000 or later environments

The Highest Resolution Mass Map of Galaxy Cluster Substructure To Date Without Assuming Light Traces Mass: LensPerfect Analysis of Abell 1689

We present a strong lensing mass model of Abell 1689 which resolves substructures ~25 kpc across (including about ten individual galaxy subhalos) within the central ~400 kpc diameter. We achieve this resolution by perfectly reproducing the observed (strongly lensed) input positions of 168 multiple images of 55 knots residing within 135 images of 42 galaxies. Our model makes no assumptions about light tracing mass, yet we reproduce the brightest visible structures with some slight deviations. A1689 remains one of the strongest known lenses on the sky, with an Einstein radius of RE = 47.0" +/- 1.2" (143 +3/-4 kpc) for a lensed source at zs = 2. We find a single NFW or Sersic prole yields a good fit simultaneously (with only slight tension) to both our strong lensing (SL) mass model and published weak lensing (WL) measurements at larger radius (out to the virial radius). According to this NFW fit, A1689 has a mass of Mvir = 2.0 +0.5/-0.3 x 10^15 Msun / h70 (M200 = 1.8 +0.4/-0.3 x 10^15 Msun / h70) within the virial radius rvir = 3.0 +/- 0.2 Mpc / h70 (r200 = 2.4 +0.1/-0.2 Mpc / h70), and a central concentration cvir = 11.5 +1.5/-1.4 (c200 = 9.2 +/- 1.2). Our SL model prefers slightly higher concentrations than previous SL models, bringing our SL+WL constraints in line with other recent derivations. Our results support those of previous studies which find A1689 has either an anomalously large concentration or significant extra mass along the line of sight (perhaps in part due to triaxiality). If clusters are generally found to have higher concentrations than realized in simulations, this could indicate they formed earlier, perhaps as a result of early dark energy.Comment: 27 pages, 17 figures, submitted to ApJ. See http://www.its.caltech.edu/~coe/LPA1689/ for complete set of color multiple images (observed and delensed) and more. Comments welcome at http://scirate.com/who.php?id=1005.xxxx&what=comments (insert arXiv number at xxxx; free & easy registration

Not In Our Backyard: Spectroscopic Support for the CLASH z=11 Candidate MACS0647-JD

We report on our first set of spectroscopic Hubble Space Telescope observations of the z~11 candidate galaxy strongly lensed by the MACSJ0647.7+7015 galaxy cluster. The three lensed images are faint and we show that these early slitless grism observations are of sufficient depth to investigate whether this high-redshift candidate, identified by its strong photometric break at ~1.5 micron, could possibly be an emission line galaxy at a much lower redshift. While such an interloper would imply the existence of a rather peculiar object, we show here that such strong emission lines would clearly have been detected. Comparing realistic, two-dimensional simulations to these new observations we would expect the necessary emission lines to be detected at >5 sigma while we see no evidence for such lines in the dispersed data of any of the three lensed images. We therefore exclude that this object could be a low redshift emission line interloper, which significantly increases the likelihood of this candidate being a bona fide z~11 galaxy.Comment: 14 Pages. 6 Figures. 2nd revised version. Accepted. To appear in ApJ. Please contact [email protected] for comments on this pape

The bright-end galaxy candidates at z ~ 9 from 79 independent HST fields

We present a full data analysis of the pure-parallel Hubble Space Telescope (HST) imaging observations in the Brightest of Reionizing Galaxies Survey (BoRG[z9]) in Cycle 22. The medium-deep exposures with five HST/WFC3IR+UVIS filter bands from 79 independent sightlines (~370 arcmin^2) provide the least biased determination of number density for z>9 bright galaxies against cosmic variance. After a strict two-step selection for candidate galaxies, including dropout color and photometric redshift analyses, and revision of previous BoRG candidates, we identify one source at z~10 and two sources at z~9. The z~10 candidate shows evidence of line-of-sight lens magnification (mu~1.5), yet it appears surprisingly luminous (MUV ~ -22.6\pm0.3 mag), making it one of the brightest candidates at z > 8 known (~ 0.3 mag brighter than the z = 8.68 galaxy EGSY8p7, spectroscopically confirmed by Zitrin and collaborators). For z ~ 9 candidates, we include previous data points at fainter magnitudes and find that the data are well fitted by a Schechter luminosity function with alpha ~ -2.1, MUV ~ -21.5 mag, and log phi ~ -4.5 Mpc^-3mag^-1, for the first time without fixing any parameters. The inferred cosmic star formation rate density is consistent with unaccelerated evolution from lower redshift.Comment: 18pages, 7figures, 6tables. accepted to the Astrophysical Journa

Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues

BACKGROUND: Formalin-fixed paraffin-embedded (FFPE) tissues represent the largest source of archival biological material available for genomic studies of human cancer. Therefore, it is desirable to develop methods that enable whole genome amplification (WGA) using DNA extracted from FFPE tissues. Multiple-strand Displacement Amplification (MDA) is an isothermal method for WGA that uses the large fragment of Bst DNA polymerase. To date, MDA has been feasible only for genomic DNA isolated from fresh or snap-frozen tissue, and yields a representational distortion of less than threefold. RESULTS: We amplified genomic DNA of five FFPE samples of normal human lung tissue with the large fragment of Bst DNA polymerase. Using quantitative PCR, the copy number of 7 genes was evaluated in both amplified and original DNA samples. Four neuroblastoma xenograft samples derived from cell lines with known N-myc gene copy number were also evaluated, as were 7 samples of non-small cell lung cancer (NSCLC) tumors with known Skp2 gene amplification. In addition, we compared the array comparative genomic hybridization (CGH)-based genome profiles of two NSCLC samples before and after Bst MDA. A median 990-fold amplification of DNA was achieved. The DNA amplification products had a very high molecular weight (> 23 Kb). When the gene content of the amplified samples was compared to that of the original samples, the representational distortion was limited to threefold. Array CGH genome profiles of amplified and non-amplified FFPE DNA were similar. CONCLUSION: Large fragment Bst DNA polymerase is suitable for WGA of DNA extracted from FFPE tissues, with an expected maximal representational distortion of threefold. Amplified DNA may be used for the detection of gene copy number changes by quantitative realtime PCR and genome profiling by array CGH

The Bright End of the z~9 and z~10 UV Luminosity Functions using all five CANDELS Fields

The deep, wide-area (~800-900 arcmin**2) near-infrared/WFC3/IR + Spitzer/IRAC observations over the CANDELS fields have been a remarkable resource for constraining the bright end of high redshift UV luminosity functions (LFs). However, the lack of HST 1.05-micron observations over the CANDELS fields has made it difficult to identify z~9-10 sources robustly, since such data are needed to confirm the presence of an abrupt Lyman break at 1.2 microns. We report here on the successful identification of many such z~9-10 sources from a new HST program (z9-CANDELS) that targets the highest-probability z~9-10 galaxy candidates with observations at 1.05 microns, to search for a robust Lyman-break at 1.2 microns. The potential z~9-10 candidates are preselected from the full HST, Spitzer/IRAC S-CANDELS observations, and the deepest-available ground-based optical+near-infrared observations. We identified 15 credible z~9-10 galaxies over the CANDELS fields. Nine of these galaxies lie at z~9 and 5 are new identifications. Our targeted follow-up strategy has proven to be very efficient in making use of scarce HST time to secure a reliable sample of z~9-10 galaxies. Through extensive simulations, we replicate the selection process for our sample (both the preselection and follow-up) and use it to improve current estimates for the volume density of bright z~9 and z~10 galaxies. The volume densities we find are 5(-2)(+3)x and 8(-3)(+9)x lower, respectively, than found at z~8. When compared with the best-fit evolution (i.e., dlog_{10} rho(UV)/dz=-0.29+/-0.02) in the UV luminosities densities from z~8 to z~4 integrated to 0.3L*(z=3) (-20 mag), these luminosity densities are 2.6(-0.9)(+1.5)x and 2.2(-1.1)(+2.0)x lower, respectively, than the extrapolated trends. Our new results are broadly consistent with the "accelerated evolution" scenario at z>8, as seen in many theoretical models.Comment: 23 pages, 15 figures, 7 tables, updated to match the version in press, including some minor textual corrections identified at the proof stag