Clinical Assessment of Nifedipine-Induced Gingival Overgrowth in a Group of Brazilian Patients

Although it has been established that nifedipine is associated with gingival overgrowth (GO), there is little information on the prevalence and severity of this condition in the Brazilian population. The aim of this study was to assess the occurrence of nifedipine-induced GO in Brazilian patients and the risk factors associated using a Clinical Index for Drug Induced Gingival Overgrowth (Clinical Index DIGO). The study was carried out on 35 patients under treatment with nifedipine (test group) and 35 patients without treatment (control group). Variables such as demographic (age, gender), pharmacological (dose, time of use), periodontal (plaque index, gingival index, probing depth, clinical insertion level, and bleeding on probing), and GO were assessed. Statistical analysis showed no association between GO and demographic or pharmacological variables. However, there was an association between GO and periodontal variables, except for plaque index. According to our study, the Clinical Index DIGO can be used as a parameter to evaluate GO. Therefore, we conclude that the presence of gingival inflammation was the main risk factor for the occurrence of nifedipine-induced GO

Efeitos da ciclosporina A sobre a sintese de proteinas de matriz extracelular de fibroblastos de gengiva normal e de portadores de fibromatose gengival hereditaria

Orientador: Sergio Roberto Peres LineTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: A fibromatose gengival pode ser hereditária ou induzida por drogas. O padrão da doença sugere que os fibroblastos gengivais podem produzir grandes volumes de substância extracelular. Um dos efeitos colaterais da ciclosporina A (CsA) é a indução de fibromatose gengival. Os efeitos de 3 diferentes concentrações de CsA sobre a morfologia, síntese de colagenase, de fibroneotina e atividade colagenolítioa, foram estudados "in vitro" em 6 linhagens de fibroblastos, 3 de gengivas normais e 3 de fibromatose gengival hereditária. Os fibroblastos foram cultivados com meio contendo concentrações de 250,500 e 1.000 ng/ml de CsA, por 24,48 e 72 h. O meio foi coletado e submetido a ensaios enzimográfioos e de atividade colagenolítica. A síntese de fibronectina foi avaliada através de "dot blot" e 4e proteína total pela análise eletroforética do sobrenadante. As diferentes concentrações de CsA não alteraram a viabilidade celular. Após 72 h. com 1.000 ng/mI de CsA todas as linhagens apresentaram alterações morfológicas. A determinação da densidade celular em câmara de Neubauer não revelou diferenças significativas na contagem celular das linhagens, quando incubadas oom diferentes concentrações de CsA...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digitalAbstract: Gingival fibromatosis may be hereditary or drug-induced. The disease features suggests that gingival fibroblasts produce high levels of extracellular matrix. The activity of the fibroblasts of gingival fibromatosis cyclosporin A-induced may be influenced by direct action of the drug on cell function. The effects of 3 different cyclosporin A (CsA) concentrations on morphology, collagenase and fibronectin synthesis and collagenolytic activity were studied in vitro using 6 fibroblasts strains, 3 of normal gingiva and 3 of hereditary gingival fibromatosis. Cell were cultured with medium containing CsA (250, 500 and 1.000 nglml) during 24, 48 and 12 h. The medium was collected and submitted to enzymographic and collagenolytic activity assays. The fibronectin synthesis was studied by dot blot analysis. Total protein synthesis was studied through electrophoretic analysis. CeUular viability was not changed by the different concentrations of CsA. After 72 h. with 1.000 ng/mI of CsA, all strains showed morphologic changes. Cellular density was determined by Neubauer chamber and no significative differences on proliferation were found between the strains when inoubated with different concentration of CsA ...Note: The complete abstract is available with the full electronic digital thesis or dissertationsDoutoradoBiologia e Patologia Buco-DentalDoutor em Ciência

Efeitos da sialoadenectomia na carcinogenese bucal de ratos provocada pelo oxido de nitroquinolina (4NQO)

Orientador : Oslei Paes de AlmeidaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Ratos normais e sialoadenectomizados receberam aplicações tópicas de óxido de nitroquinolina (4NQO) a 0,5Y. em propilenoglicol no palato, quatro vezes por semana, durante um, dois, três e seis meses. Macroscopicamente o palato apresentou esbranquiçamento, perda de definição das rugosidades, aspecto papilomatoso e ulcerações deformantes com mobilidade dentária. Na língua, pode-se verificar atrofia de papilas, 'papilomas e ulcerações. Microscopicamente foram observadas alterações na queratinização, presença de disqueratose focal acantolítica, e carcinomas superficial e profundamente invasivos. Tanto no palato como na língua, as alterações foram mais precoces e mais intensas nos sialoadenectomizados; indicando que a secreção das glândulas salivares maiores tem efeito protetor no desenvolvimento da carcinogênese bucal provocada pelo 4NQOAbstract: Normal and sialoadenectomi2ed Wistar rats treated on the palate with 4-n it roquinol ine oxide 0,SY. in propyleneglicol four times weekl during 1, 2, 3 and 6 months. Macroscopicallythe palate showed whitish aspect, loss of rugosities definition, papillomatous projections an ulcerations with dental mobility. The dorso of the tongue presented papil1ae atrophy, papillomas and ulcerations. The main microscopical.features were hiperqueratosis, focal acantolytic dyskeratosis and differentiated squamous cell carcinoma. Either on the palate and the tongue the alterations were more pronounced and numerous on sialoadenectomi2ed rats indicating that secretion of the major salivary glands are effective to protect the oral mucosa of the rat of the effects of the cprcinogen 4-nitroquinoline-oxideMestradoMestre em Biologia e Patologia Buco-Denta

Hallazgos clínicos, imagenológicos y anatomopatológicos en pacientes fallecidos con diagnóstico presuntivo de tromboembolismo pulmonar

Introducción: el tromboembolismo pulmonar es frecuente, potencialmente letal y puede tener dificultades diagnósticas.Objetivo: caracterizar clínica, imagenológica y anatopatológicamente los pacientes fallecidos con diagnóstico presuntivo de tromboembolismo pulmonar.Material y método: se realizó un estudio descriptivo transversal de los fallecidos con diagnóstico presuntivo de tromboembolismo pulmonar desde octubre de 2018 a octubre de 2019, en el Hospital Provincial Saturnino Lora. La muestra quedó conformada por 120 fallecidos. Se describieron variables como edad, sexo, factores predisponentes,  principal síntoma o signo clínico,  radiográfico y ecocardiográfico sugestivo de tromboembolismo así como el diagnóstico anatomopatológico confirmado o no de tromboembolismo.Resultados: predominó los pacientes mayores de 60 años y el sexo femenino. La taquicardia (25,0%) y la disnea (33,3%) fueron los síntomas y signos más frecuentes en los casos confirmados. Según diagnóstico patológico predominó el infarto pulmonar en el 79,2% y el D-Dímero resultó positivo en todos los casos. Prevalecieron las aéreas radiológicas opacas (78,9%), la taquicardia sinusal (37,5%) y la dilatación y/o hipoquinesia del ventrículo derecho (45,5%)Conclusión: El diagnóstico presuntivo de tromboembolismo y el diagnóstico anatomopatológico no fue coincidente en un número considerable de fallecidos

Effects of Exogenous Enzymes and Salix babylonica L. Extract on Cellular Immune Response and its Correlation with Average Daily Weight Gain in Growing Lambs

The aim of this study was to determine the effects of exogenous enzyme (EZ) and Salix babylonica L. (SB) extract on cellular immune response, and its correlation with average daily weight gain (ADG). Twenty Suffolk lambs, 6-8 months of age and average live weight of 24±0.3 kg, were used in a trial which lasted 60 days. The lambs were distributed into 4 groups of 5 lambs each and housed in individual 1.5x1.5 m cages in a completely randomized design. The treatments were: (i) Control; lambs consuming basal diet (BD) only; (ii) EZ; lambs consuming BD plus 10g of EZ (ZADO®); (iii) SB; lambs consuming BD plus 30 mL of SB, and (iv) EZSB; lambs consuming BD plus 10g EZ and 30 mL of SB. Blood samples were collected on days 0, 15, 30, 45 and 60 and analysed for helper T lymphocytes, cytotoxic T limphocytes, granulocytes and monocytes by flow cytometry. Treatments had no effect on parameters measured, but day of sampling had linear and cubic effects on helper T lymphocytes, granulocytes and monocytes (P<0.01) and cubic effects on cytotoxic T lymphocytes (P<0.01). The results suggest that EZ and SB have immunosuppressant effects in the first 15 days, after this effect were immunosuppressive on cytotoxic T lymphocytes and granulocytes, for monocytes the effect was immunostimulant. No there were correlation between ADG and cellular immune response in this experiment

Comparison of manual, digital and lateral CBCT cephalometric analyses

Objective: The aim of this study was to compare the reliability of three different methods of cephalometric analysis. Material and Methods: Conventional pretreatment lateral cephalograms and cone beam computed tomography (CBCT) scans from 50 subjects from a radiological clinic were selected in order to test the three methods: manual tracings (MT), digitized lateral cephalograms (DLC), and lateral cephalograms from CBCT (LC-CBCT). The lateral cephalograms were manually analyzed through the Dolphin Imaging 11.0™ software. Twenty measurements were performed under the same conditions, and retraced after a 30-day period. Paired t tests and the Dahlberg formula were used to evaluate the intra-examiner errors. The Pearson's correlation coefficient and one-way analysis of variance (ANOVA) tests were used to compare the differences between the methods. Results: Intra-examiner reliability occurred for all methods for most of the measurements. Only six measurements were different between the methods and an agreement was observed in the analyses among the 3 methods. Conclusions: The results demonstrated that all evaluated methodologies are reliable and valid for scientific research, however, the method used in the lateral cephalograms from the CBCT proved the most reliable

New diagnoses of human immunodeficiency virus infection in the Spanish pediatric HIV Cohort (CoRISpe) from 2004 to 2013

Vertical human immunodeficiency virus (HIV) infection has decreased in industrialized countries in recent decades, but there are no studies on the mechanisms of HIV transmission among infected children in Spain. Our aim was to study the characteristics and trends of diagnoses of vertically HIV-infected children in Spain from 2004 to 2013. Vertically HIV-infected children were selected if they were diagnosed from 2004 to 2013, were aged 0 to 18 years old, and were included in the Cohort of the Spanish Pediatric HIV Network (CoRISpe). Demographic, clinical, immunological, and virological data at diagnosis were obtained. The rate of diagnoses of vertically HIV-infected children was calculated as the number of cases per 100,000 inhabitants. Obstetric data of mothers of Spanish children and prophylaxis at childbirth and postpartum were obtained. A total of 218 HIV-infected children were included in the study. Of this sample, 182 children (83.5%) were perinatally HIV infected, and 125 out of those 182 children (68.7%) were born in Spain. The vertically HIV-infected Spanish children were diagnosed earlier and were in better clinical and immunological condition at diagnosis than were foreign children. The rate of vertically HIV-infected children declined from 0.09 in 2004 to 0.03 in 2013 due to the decrease in the rate of children born in Spain (0.08 in 2004 vs 0.01 in 2013). A total of 60 out of 107 mothers (56.1%) of Spanish children were diagnosed at or after childbirth. However, this number declined between 2004 and 2013. The rate of new HIV diagnoses of vertically HIV-infected children decreased significantly between 2004 and 2013 from 0.09 to 0.03 per 100,000 inhabitant

New diagnoses of human immunodeficiency virus infection in the Spanish pediatric HIV Cohort (CoRISpe) from 2004 to 2013

Vertical human immunodeficiency virus (HIV) infection has decreased in industrialized countries in recent decades, but there are no studies on the mechanisms of HIV transmission among infected children in Spain. Our aim was to study the characteristics and trends of diagnoses of vertically HIV-infected children in Spain from 2004 to 2013. Vertically HIV-infected children were selected if they were diagnosed from 2004 to 2013, were aged 0 to 18 years old, and were included in the Cohort of the Spanish Pediatric HIV Network (CoRISpe). Demographic, clinical, immunological, and virological data at diagnosis were obtained. The rate of diagnoses of vertically HIV-infected children was calculated as the number of cases per 100,000 inhabitants. Obstetric data of mothers of Spanish children and prophylaxis at childbirth and postpartum were obtained. A total of 218 HIV-infected children were included in the study. Of this sample, 182 children (83.5%) were perinatally HIV infected, and 125 out of those 182 children (68.7%) were born in Spain. The vertically HIV-infected Spanish children were diagnosed earlier and were in better clinical and immunological condition at diagnosis than were foreign children. The rate of vertically HIV-infected children declined from 0.09 in 2004 to 0.03 in 2013 due to the decrease in the rate of children born in Spain (0.08 in 2004 vs 0.01 in 2013). A total of 60 out of 107 mothers (56.1%) of Spanish children were diagnosed at or after childbirth. However, this number declined between 2004 and 2013. The rate of new HIV diagnoses of vertically HIV-infected children decreased significantly between 2004 and 2013 from 0.09 to 0.03 per 100,000 inhabitants

A new tool for the paediatric HIV research:general data from the Cohort of the SpanishPaediatric HIV Network (CoRISpe)

There are approximately from 1,100 to 1,200 HIV-infected children in a follow-up in Spain. In 2008 an open, multicentral, retrospective and prospective Cohort of the Spanish Paediatric HIV Network (CoRISpe) was founded. The CoRISpe is divided into the node 1 and node 2 representing geographically almost the whole territory of Spain. Since 2008 seventy-five hospitals have been participating in the CoRISpe. All the retrospective data of the HIV-infected children have been kept in the CoRISpe since 1995 and prospective data since 2008. In this article we are going to present the notion of CoRISpe, its role, the structure, how the CoRISpe works and the process how a child is transferred from Paediatric to Adults Units. The main objective of the CoRISpe is to contribute to furthering scientific knowledge on paediatric HIV infection by providing demographic, sociopsychological, clinical and laboratory data from HIV-infected paediatric patients. Its aim is to enable high-quality research studies on HIV-infected children

CARACTERIZAÇÃO SOCIODEMOGRÁFICA DAS NOTIFICAÇÕES DE VIOLÊNCIA INTERPESSOAL EM MULHERES EM IDADE FÉRTIL EM UM HOSPITAL DE ENSINO NO PARANÁ

Objetivo: O objetivo do estudo foi descrever características sociodemográficas das violências interpessoais em mulheres em idade fértil, notificadas em um hospital de ensino público no período de 2014 a 2018. Método: estudo documental, retrospectivo, descritivo, com análise quantitativa, com base em dados do Sistema de Informação de Agravos de Notificação (SINAN). Resultados: Ocorreram 883 (100%) violências contra mulheres, destas 314 (35,5%) para mulheres em idade fértil (10 a 49 anos), sendo 130 (41,4%) na faixa etária de 10 a 19 anos, ou seja, na adolescência . Do total 197 (62,7%) eram brancas, 302 (96,1%) residentes em região urbana, 302 (96,1%) com ensino fundamental incompleto e 125 (39,8%) solteiras. A autoria da agressão teve predominância para sexo masculino 197 (62,7%), e o local de ocorrência foi a residência da vítima 185 (64,9%). Conclusão: A prevenção da violência contra mulher é possível por meio da eliminação de condições que propiciem o ato, como a superação da desigualdade social em que a mulher está culturalmente inserida e elaboração de políticas públicas fundamentais com a inclusão de pautas que discutam a vulnerabilidade do gênero feminino, a parir de estudos que subsidiem a elaboração e a avaliação dessas políticas