Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis

Background The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B. mallei is a recently emerged, host restricted clone of B. pseudomallei. Results Using bacteriophage-mediated immunoscreening we identified genes expressed in vivo during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated Burkholderia Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a B. mallei expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a B. pseudomallei expression library screened with serum from 21 patients with culture-proven melioidosis. Conclusion Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both B. mallei and B. pseudomallei contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to B. mallei and their potential as diagnostics for glanders requires further study

Quantum Chaos in Open versus Closed Quantum Dots: Signatures of Interacting Particles

This paper reviews recent studies of mesoscopic fluctuations in transport through ballistic quantum dots, emphasizing differences between conduction through open dots and tunneling through nearly isolated dots. Both the open dots and the tunnel-contacted dots show random, repeatable conductance fluctuations with universal statistical proper-ties that are accurately characterized by a variety of theoretical models including random matrix theory, semiclassical methods and nonlinear sigma model calculations. We apply these results in open dots to extract the dephasing rate of electrons within the dot. In the tunneling regime, electron interaction dominates transport since the tunneling of a single electron onto a small dot may be sufficiently energetically costly (due to the small capacitance) that conduction is suppressed altogether. How interactions combine with quantum interference are best seen in this regime.Comment: 15 pages, 11 figures, PDF 2.1 format, to appear in "Chaos, Solitons & Fractals

Spectra of Coherent Smith-Purcell Radiation Observed from Short Electron Bunches: Numerical and Experimental Studies

MOPWA056 - ISBN978-3-95450-122-9International audienceThere is a significant interest in the development of compact particle accelerators within research areas including X-ray and THz (T-ray) sources of radiation, particle physics and medical sciences. To support the progress in these areas, non-invasive, electron beam diagnostics that are capable of measuring a single femtosecond electron bunch are required. At the current stage such beam diagnostics for femtosecond-long electron bunches are still not available. The goal of the work presented is to understand the spectral characteristics of coherent Smith-Purcell radiation to enable its quick and reliable interpretation including the longitudinal profile reconstruction of electron bunches. The research presented comprises results from numerical modelling and experimental studies. Using the numerical data, we discuss the radiated spectra dependence on the electron bunch profile and analyse the results. We also discuss the experimental data and compare it with theoretical predictions

Dynamics of tournaments: the soccer case

A random walk-like model is considered to discuss statistical aspects of tournaments. The model is applied to soccer leagues with emphasis on the scores. This competitive system was computationally simulated and the results are compared with empirical data from the English, the German and the Spanish leagues and showed a good agreement with them. The present approach enabled us to characterize a diffusion where the scores are not normally distributed, having a short and asymmetric tail extending towards more positive values. We argue that this non-Gaussian behavior is related with the difference between the teams and with the asymmetry of the scores system. In addition, we compared two tournament systems: the all-play-all and the elimination tournaments.Comment: To appear in EPJ

Detector for imaging and dosimetry of laser-driven epithermal neutrons by alpha conversion

An epithermal neutron imager based on detecting alpha particles created by boron neutron capture mechanism is discussed. The diagnostic mainly consists of a mm thick Boron Nitride (BN) sheet (as an alpha converter) in contact with a non-borated cellulose nitride film (LR115 type-II) detector. While the BN absorbs the neutrons below 0.1 eV, the fast neutrons register insignificantly in the detector due to their low neutron capture and recoil cross-sections. The use of solid-state nuclear track detectors (SSNTD), unlike image plates, micro-channel plates and scintillators, provide safeguard from the x-rays, gamma-rays and electrons. The diagnostic was tested on a proof-of-principle basis, in front of a laser driven source of moderated neutrons, which suggests the potential of using this diagnostic (BN+SSNTD) for dosimetry and imaging applications

Numerical study of neutron beam divergence in a beam-fusion scenario employing laser driven ions

The most established route to create a laser-based neutron source is by employing laser accelerated, low atomic number ions in fusion reactions. In addition to benefiting from the high reaction cross-sections at moderate energies of the projectile ions, the anisotropy in the neutron emission is another important feature of beam-fusion reactions. Using a simple numerical model based on neutron generation in a pitcher-catcher scenario, anisotropy in the neutron emission was studied for the deuterium-deuterium fusion reaction. Simulation results are consistent with the narrow divergence ( 70 full width at half maximum) neutron beam recently obtained from an experiment employing multi-MeV deuteron beams of narrow divergence (upto 30 FWHM depending on the ion energy) accelerated by a subpetawatt laser pulse from thin deuterated plastic foils via the Target Normal Sheath Acceleration mechanism. By varying the input ion beam parameters, simulations show that a further improvement in the neutron beam directionality (i.e. reduction in the beam divergence) can be obtained by increasing the projectile ion beam temperature and cut-off energy, as expected from the interactions with higher power lasers at upcoming facilities

Software engineering techniques for the development of systems of systems

This paper investigates how existing software engineering techniques can be employed, adapted and integrated for the development of systems of systems. Starting from existing system-of-systems (SoS) studies, we identify computing paradigms and techniques that have the potential to help address the challenges associated with SoS development, and propose an SoS development framework that combines these techniques in a novel way. This framework addresses the development of a class of IT systems of systems characterised by high variability in the types of interactions between their component systems, and by relatively small numbers of such interactions. We describe how the framework supports the dynamic, automated generation of the system interfaces required to achieve these interactions, and present a case study illustrating the development of a data-centre SoS using the new framework

Initiation of the West Antarctic Ice Sheet and estimates of total Antarctic ice volume in the earliest Oligocene

Reconstructions of Antarctic paleotopography for the late Eocene suggest that glacial erosion and thermal subsidence have lowered West Antarctic elevations considerably since then, with Antarctic land area having decreased ~20%. A new climate-ice sheet model based on these reconstructions shows that the West Antarctic Ice Sheet first formed at the Eocene-Oligocene transition (33.8–33.5 Ma, E-O) in concert with the continental-scale expansion of the East Antarctica Ice Sheet and that the total volume of East and West Antarctic ice (33.4–35.9 × 106 km3) was >1.4 times greater than previously assumed. This larger modeled ice volume is consistent with a modest cooling of 1–2°C in the deep ocean during the E-O transition, lower than other estimates of ~3°C cooling, and suggests the possibility of substantial ice in the Antarctic interior before the Eocene-Oligocene boundary