Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates

One of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic raw material and waste towards biogas by highly diverse microbial consortia. In this context, the microbial systems ecology of thermophilic industrial-scale biogas plants is poorly understood

Genomics and prevalence of bacterial and archaeal isolates from biogas-producing microbiomes

Background: To elucidate biogas microbial communities and processes, the application of high-throughput DNA analysis approaches is becoming increasingly important. Unfortunately, generated data can only partialy be interpreted rudimentary since databases lack reference sequences. Results: Novel cellulolytic, hydrolytic, and acidogenic/acetogenic Bacteria as well as methanogenic Archaea originating from different anaerobic digestion communities were analyzed on the genomic level to assess their role in biomass decomposition and biogas production. Some of the analyzed bacterial strains were recently described as new species and even genera, namely Herbinix hemicellulosilytica T3/55T, Herbinix luporum SD1DT, Clostridium bornimense M2/40T, Proteiniphilum saccharofermentans M3/6T, Fermentimonas caenicola ING2-E5BT, and Petrimonas mucosa ING2-E5AT. High-throughput genome sequencing of 22 anaerobic digestion isolates enabled functional genome interpretation, metabolic reconstruction, and prediction of microbial traits regarding their abilities to utilize complex bio-polymers and to perform specific fermentation pathways. To determine the prevalence of the isolates included in this study in different biogas systems, corresponding metagenome fragment mappings were done. Methanoculleus bourgensis was found to be abundant in three mesophilic biogas plants studied and slightly less abundant in a thermophilic biogas plant, whereas Defluviitoga tunisiensis was only prominent in the thermophilic system. Moreover, several of the analyzed species were clearly detectable in the mesophilic biogas plants, but appeared to be only moderately abundant. Among the species for which genome sequence information was publicly available prior to this study, only the species Amphibacillus xylanus, Clostridium clariflavum, and Lactobacillus acidophilus are of importance for the biogas microbiomes analyzed, but did not reach the level of abundance as determined for M. bourgensis and D. tunisiensis. Conclusions: Isolation of key anaerobic digestion microorganisms and their functional interpretation was achieved by application of elaborated cultivation techniques and subsequent genome analyses. New isolates and their genome information extend the repository covering anaerobic digestion community members. © 2017 The Author(s)

Personal factors and their relevance for the assessment and allocation of benefits in social medicine and rehabilitation

A broad and common understanding of the nature of Personal Factors contributes and initiates a dialogue between professionals involved in health care to gain a comprehensive perspective regarding an individual’s health condition and accurately allocate social benefits and medical interventions. Personal Factors play an essential role in the International Classification of Functioning, Disability and Health (ICF) and the underlying Bio-Psycho-Social Disease Model. To date, the World Health Organisation (WHO) has not classified Personal Factors for global use despite their impact on the functioning of individuals as facilitators or barriers. This editorial wants to outline the importance of Personal Factors, and stimulate a discussion about the need to fulfil this component by creating a commonly accepted list of item

PERSONAL FACTORS AND THEIR PERSPECTIVE IN SOCIAL MEDICINE AND REHABILITATION

Motivation / Background: A broad and common understanding of the nature of Personal Factors is deemed to be necessary to gain and assess a comprehensive perspective regarding an individual’s health condition and accurately allocate social and medical benefits and interventions. Personal Factors have an impact on the functioning of individuals as facilitators or barriers. They play an essential role in the International Classification of Functioning, Disability and Health (ICF). To date, the World Health Organisation has not classified Personal Factors for global use. Method: A consensus group representing a broad spectrum of medical and social security professionals, research societies together with representatives of self-help organisations developed and published a proposal to classify relevant Personal Factors. Results: To face ongoing discussions misunderstandings are addressed, critics and suggestions balanced and positions created with pointed explanations and supplemented literature. Core messages summarise each position in a condensed form. Conclusions: Personal Factors may exert a powerful influence on the goal definition, goal achievement and type, complexity and length of necessary medical, social or rehabilitation benefits. Transparency, explicitness, fairness (standardisation) and the possibility for well-founded claims comprise additional arguments for reporting Personal Factors. They may be crucial to reach the goal of the highest degree of participation considering individual resources

Personal Factors Classification Revisited : A Proposal in the Light of the Biopsychosocial Model of the World Health Organization (WHO)

In 2010, we proposed a personal factor classification which was published in this journal. Since then, the International Classification of Functioning, Disability and Health (ICF) of the World Health Organization (WHO) and the biopsychosocial model were increasingly incorporated into the German Social Law Code for participation and rehabilitation, implying that personal factors are indispensable for individual assessments. For the present study, we aimed to come up with an updated version of the personal factors classification based on current research. To achieve this goal, we employed a qualitative approach to re-examine the basic structure, consistency, and selection of categories in the classification from our 2010 study, to amend and supplement the categories to reflect best practice personal factor classifications. Our findings indicate that the basic structure remained largely unchanged, with relatively minor changes, including the deletion of 5 categories from our 2010 classification, 10 categories revised in format or content, and 13 new categories. We believe our revised classification to be useful for supporting users in systematically, comprehensively, and transparently reporting influences on specific aspects of individuals’ life and living background on their functioning and participation, thus facilitating an equitable allocation of disability benefits

Aster asa-grayi Makino

原著和名: イソノギク科名: キク科 = Compositae採集地: 沖縄県 沖縄本島 国頭郡 恩納村 万座毛付近 (琉球 沖縄本島 国頭郡 恩納村 万座毛付近)採集日: 1986/2/23採集者: 萩庭丈壽整理番号: JH009722国立科学博物館整理番号: TNS-VS-95972

Příspěvek k výskytu a genesi tonsteinů v uhelných pánvích

Additional file 2. Genomic loci encoding enzymatic functions participating in the propionic acid, ethanol, formic acid, butyric acid and lactic acid fermentation for each strain analyzed