Software Development and Testing: Approach and Challenges in a distributed HEP Collaboration

In developing the ATLAS [1] Trigger and Data Acquisition (TDAQ) software, the team is applying the iterative waterfall model, evolutionary process management, formal software inspection, and lightweight review techniques. The long preparation phase, with a geographically widespread development team required that the standard techniques be adapted to this HEP environment. The testing process is receiving special attention. Unit tests and check targets in nightly project builds form the basis for the subsequent software project release testing. The integrated software is then being run on computing farms that give further opportunites for gaining experience, fault finding, and acquiring ideas for improvement. Dedicated tests on a farm of up to 1000 nodes address the large-scale aspect of the project. Integration test activities on the experimental site include the special purpose-built event readout hardware. Deployment in detector commissioning starts the countdown towards running the final ATLAS experiment. These activities aim at both understanding and completing the complex system, and help in forming a team whose members have a variety of expertise, working cultures, and professional backgrounds

Status and Duties of Voluntary Fire Services in the Integrated Rescue System

Príspevok je pohľadom na dobrovoľné hasičské jednotky v zriaďovateľskej kompetencii samosprávy. Obsahovo je ho možné rozdeliť na dve základné časti. Prvú predstavuje zaradenie týchto jednotiek do integrovaného záchranného systému. V druhej sa rozoberajú základné požiadavky na akcieschopnosť uvedených jednotiek a ich perspektívu.The paper provides an introduction of voluntary fire units established by the local self-government authority. In content regard, it was divided into two basic parts. The first part includes the categorisation of these fire services in the integrated rescue system. In the next part, the basic requirements for the fighting power of voluntary fire services above and their perspectives are discussed

Bucket elevator

Tato bakalářská práce se zabývá návrhem svislého korečkového elevátoru a vypracováním jeho technické zprávy. Dopravovaným materiálem je obilí. Práce je rozdělená na několik kapitol, z nichž první se zabývá obecným popisem elevátoru, jeho konstrukčním řešením a pohonem. Na tuto teoretickou část dále navazují kapacitní výpočty. Na závěr je návrh ověřen kontrolou hřídele na ohyb a krut.This bechalor thesis deals with design of vertical bucket elevator and formulate its technical report. Transported material is grain. The thesis is divided into several chapters, the first of which deals with a general description of the elevator, its structural design and drive. This theoretical part also build capacity calculations. In conclusion, the proposal is verified by checking the shaft to bend and twist.

Analysis of Steam Parameters for ZEVO

Import 02/11/2016Tato diplomová práce se zabývá analýzou vlivu parametrů páry na ZEVO a její následné porovnání s hnědouhelnou elektrárnou. Práce je rozdělená na několik kapitol, z nichž první popisuje SAKO Brno a její princip. Na tuto teoretickou část dále navazují nezbytné výpočty. Na závěr jsou přiloženy schematické výkresy pro ZEVO, aby zařízení vyhovovalo změněným parametrům páry.The diploma thesis focuses on the analysis of steam for ZEVO and its following comparison with brown coal power plant. The work is divided into several chapters, the first describes SAKO Brno and its principle. This theoretical part continues with the necessary calculations. In conclusion are attached schematic drawings for ZEVO to suit the changed vapor parameters.361 - Katedra energetikydobř

Implementation of type traning in FTO

Cílem této bakalářské práce je podrobně rozebrat postup pro získání statusu TRTO. Jsou uvedeny jednotlivé kroky od poţadavků nutných k získání Osvědčení a udrţení aţ po okolnosti za kterých zaniká. Dále je popsán samotný výcvik pro letoun Super King Air B200 a jsou sestaveny osnovy typového výcviku.The aim of this bachelor’s thesis is to cover in detail the procedure for getting TRTO status. There are given certain steps from necessary requirements for obtaining the Certificate and keeping it, to circumstances that can cause its expiration. The course itself is described for Super King Air B200 aircraft and type rating plans are made.

Hyponatraemia despite isotonic maintenance fluid therapy: a time series intervention study

OBJECTIVE: To examine the prevalence of dysnatraemias among children admitted for paediatric surgery before and after a change from hypotonic to isotonic intravenous maintenance fluid therapy. DESIGN: Retrospective consecutive time series intervention study. SETTING: Paediatric surgery ward at the Children's Hospital in Lund, during a 7-year period, 2010-2017. PATIENTS: All children with a blood sodium concentration measurement during the study period were included. Hypotonic maintenance fluid (40 mmol/L NaCl and 20 mmol/L KCl) was used during the first 3 years of the study (646 patients), and isotonic solution (140 mmol/L NaCl and 20 mmol/L KCl) was used during the following period (807 patients). MAIN OUTCOME MEASURES: Primary outcomes were sodium concentration and occurrence of hyponatraemia (145 mmol/L). RESULTS: Overall, the change from hypotonic to isotonic intravenous maintenance fluid therapy was associated with a decreased prevalence of hyponatraemia from 29% to 22% (adjusted OR 0.65 (0.51-0.82)) without a significantly increased odds for hypernatraemia (from 3.4% to 4.3%, adjusted OR 1.2 (0.71-2.1)). Hyponatraemia <130 mmol/L decreased from 6.2% to 2.6%, and hyponatraemia <125 mmol/L decreased from 2.0% to 0.5%. CONCLUSIONS: Routine use of intravenous isotonic maintenance fluids was associated with lower prevalence of hyponatraemia, although hyponatraemia still occurred in over 20% of patients. We propose that the composition and the volume of administered fluid need to be addressed

A Novel Mechanism of Bacterial Toxin Transfer within Host Blood Cell-Derived Microvesicles.

Shiga toxin (Stx) is the main virulence factor of enterohemorrhagic Escherichia coli, which are non-invasive strains that can lead to hemolytic uremic syndrome (HUS), associated with renal failure and death. Although bacteremia does not occur, bacterial virulence factors gain access to the circulation and are thereafter presumed to cause target organ damage. Stx was previously shown to circulate bound to blood cells but the mechanism by which it would potentially transfer to target organ cells has not been elucidated. Here we show that blood cell-derived microvesicles, shed during HUS, contain Stx and are found within patient renal cortical cells. The finding was reproduced in mice infected with Stx-producing Escherichia coli exhibiting Stx-containing blood cell-derived microvesicles in the circulation that reached the kidney where they were transferred into glomerular and peritubular capillary endothelial cells and further through their basement membranes followed by podocytes and tubular epithelial cells, respectively. In vitro studies demonstrated that blood cell-derived microvesicles containing Stx undergo endocytosis in glomerular endothelial cells leading to cell death secondary to inhibited protein synthesis. This study demonstrates a novel virulence mechanism whereby bacterial toxin is transferred within host blood cell-derived microvesicles in which it may evade the host immune system

Vitamin D Induction of the Human Antimicrobial Peptide Cathelicidin in the Urinary Bladder

The urinary tract is frequently being exposed to potential pathogens and rapid defence mechanisms are therefore needed. Cathelicidin, a human antimicrobial peptide is expressed and secreted by bladder epithelial cells and protects the urinary tract from infection. Here we show that vitamin D can induce cathelicidin in the urinary bladder. We analyzed bladder tissue from postmenopausal women for expression of cathelicidin, before and after a three-month period of supplementation with 25-hydroxyvitamin D3 (25D3). Cell culture experiments were performed to elucidate the mechanisms for cathelicidin induction. We observed that, vitamin D per se did not up-regulate cathelicidin in serum or in bladder tissue of the women in this study. However, when the bladder biopsies were infected with uropathogenic E. coli (UPEC), a significant increase in cathelicidin expression was observed after 25D3 supplementation. This observation was confirmed in human bladder cell lines, even though here, cathelicidin induction occurred irrespectively of infection. Vitamin D treated bladder cells exerted an increased antibacterial effect against UPEC and colocalization to cathelicidin indicated the relevance of this peptide. In the light of the rapidly growing problem of resistance to common urinary tract antibiotics, we suggest that vitamin D may be a potential complement in the prevention of UTI

Peptides and Peptidomimetics for Antimicrobial Drug Design

The purpose of this paper is to introduce and highlight a few classes of traditional antimicrobial peptides with a focus on structure-activity relationship studies. After first dissecting the important physiochemical properties that influence the antimicrobial and toxic properties of antimicrobial peptides, the contributions of individual amino acids with respect to the peptides antibacterial properties are presented. A brief discussion of the mechanisms of action of different antimicrobials as well as the development of bacterial resistance towards antimicrobial peptides follows. Finally, current efforts on novel design strategies and peptidomimetics are introduced to illustrate the importance of antimicrobial peptide research in the development of future antibiotics