Direct Democracy and Minority Rights: Direct and Indirect Effects on Religious Minorities in Switzerland

Most of the research on the effects of direct democracy on minority rights is empirically limited to the direct effects of direct democracy. This article takes the issue a step further and examines both direct and indirect effects by investigating the rights of religious minorities in Switzerland. The analysis provides two main insights: all direct effects are negative and can be observed when the rights of out-groups like Islamic minorities are at stake. Second, indirect effects on the parliamentary process can be observed, too: parliaments make laws more restrictive toward Islamic minorities if they fear a popular vote. However, they develop strategies to enforce their liberal interests, as shown by the fact that extensions of the rights of religious minorities are passed in total revision

Is there a bias towards elected actors in media coverage of policy-making in European metropolitan areas?

Metropolitan areas are a typical and increasingly discussed example for fragmented governance lacking clear accountability structures. While most of the literature has focused on the legitimacy of institutions and actors so far, we add a communicational dimension to democratic accountability. In this regard we argue that public accountability/ or accountability through the mass media has become a democratic standard that can be measured/ analyzed. Comparing two types of metropolitan governance in four countries, we test a theoretical and an empirical hypothesis regarding media’s ability to be a forum for public accountability. First, we argue that the media points out who is responsible for policy decisions in a way that mirrors governance structures. By pointing out who is responsible for policy making processes, public accountability reduces the complexity of network governance and actors can be held accountable. Second, however, we expect that the media are biased in focusing more on elected actors because due to their institutional/ political accountability they are more visible and thus easier to be held accountable. Analysing newspaper content data, we come to a mixed conclusion. While both elected and non-elected policy-actors are visible in the media, elected actors are more often attributed with responsibility and blamed than they are actually responsible. Unelected actors are not only outside of the chain of delegation in governance structures, they are also less in the focus of public control. Hence, although non-elected actors are less in the focus of public control, the mass media compensate their institutional lack of accountability by holding them accountable in public

The communicational dimension of democratic accountability in metropolitan governance: Media reporting and perceived legitimacy in four European cities

As cities grow and expand, complex network governance (advocated by the so-called ‘new regionalism’) is increasingly important for policy-making in metropolitan areas. These arrangements have often been criticised as a threat to legitimacy, as they involve a wide array of policy-actors and blurrs and dilute electoral accountability. This paper focuses on the communicational dimension of democratic accountability in metropolitan governance, by exploring the role of the media. We use data from a standardized content analysis of newspaper coverage on metropolitan policy-making in four European mega- and metacities (Paris, London, Berlin and Zurich) and examine their relationship to legitimacy perceptions at the individual level on the basis of survey data. We find that institutional differences in metropolitan governance are quite adequately reflected in media reports. The results also show that media content indeed is correlated with citizen perceptions of legitimacy, i.e. trust in government as well as satisfaction with democracy. We therefore interpret the media as an additional - communicational – channel of democratic accountability in metropolitan governance

Direct democracy and minority rights: direct and indirect effects on religious minorities in Switzerland

Most of the research on the effects of direct democracy on minority rights is empirically limited to the direct effects of direct democracy. This article takes the issue a step further and examines both direct and indirect effects by investigating the rights of religious minorities in Switzerland. The analysis provides two main insights: all direct effects are negative and can be observed when the rights of out-groups like Islamic minorities are at stake. Second, indirect effects on the parliamentary process can be observed, too: parliaments make laws more restrictive toward Islamic minorities if they fear a popular vote. However, they develop strategies to enforce their liberal interests, as shown by the fact that extensions of the rights of religious minorities are passed in total revisions

Direct Democracy and Minority Rights: Direct and Indirect Effects on Religious Minorities in Switzerland

Most of the research on the effects of direct democracy on minority rights is empirically limited to the direct effects of direct democracy. This article takes the issue a step further and examines both direct and indirect effects by investigating the rights of religious minorities in Switzerland. The analysis provides two main insights: all direct effects are negative and can be observed when the rights of out-groups like Islamic minorities are at stake. Second, indirect effects on the parliamentary process can be observed, too: parliaments make laws more restrictive toward Islamic minorities if they fear a popular vote. However, they develop strategies to enforce their liberal interests, as shown by the fact that extensions of the rights of religious minorities are passed in total revisions

Binding pocket stabilization by high-throughput screening of yeast display libraries

Protein dynamics have a great influence on the binding pockets of some therapeutic targets. Flexible protein binding sites can result in transient binding pocket formation which might have a negative impact on drug screening efforts. Here, we describe a protein engineering strategy with FK506-binding protein 51 (FKBP51) as a model protein, which is a promising target for stress-related disorders. High-throughput screening of yeast display libraries of FKBP51 resulted in the identification of variants exhibiting higher affinity binding of conformation-specific FKBP51 selective inhibitors. The gene libraries of a random mutagenesis and site saturation mutagenesis of the FK1 domain of FKBP51 encoding sequence were used to create a yeast surface display library. Fluorescence-activated cell sorting for FKBP51 variants that bind conformation-specific fluorescently labeled ligands with high affinity allowed for the identification of 15 different protein variants with improved binding to either, or both FKBP51-specific ligands used in the screening, with improved affinities up to 34-fold compared to the wild type. These variants will pave the way to a better understanding of the conformational flexibility of the FKBP51 binding pocket and may enable the isolation of new selective ligands that preferably and selectively bind the active site of the protein in its open conformation state

Does abscisic acid affect strigolactone biosynthesis?

Strigolactones are considered a novel class of plant hormones that, in addition to their endogenous signalling function, are exuded into the rhizosphere acting as a signal to stimulate hyphal branching of arbuscular mycorrhizal (AM) fungi and germination of root parasitic plant seeds. Considering the importance of the strigolactones and their biosynthetic origin (from carotenoids), we investigated the relationship with the plant hormone abscisic acid (ABA). Strigolactone production and ABA content in the presence of specific inhibitors of oxidative carotenoid cleavage enzymes and in several tomato ABA-deficient mutants were analysed by LC-MS/MS. In addition, the expression of two genes involved in strigolactone biosynthesis was studied. * • The carotenoid cleavage dioxygenase (CCD) inhibitor D2 reduced strigolactone but not ABA content of roots. However, in abamineSG-treated plants, an inhibitor of 9-cis-epoxycarotenoid dioxygenase (NCED), and the ABA mutants notabilis, sitiens and flacca, ABA and strigolactones were greatly reduced. The reduction in strigolactone production correlated with the downregulation of LeCCD7 and LeCCD8 genes in all three mutants. * • The results show a correlation between ABA levels and strigolactone production, and suggest a role for ABA in the regulation of strigolactone biosynthesis

Self-decorating cells via surface-initiated enzymatic controlled radical polymerization

Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering

A New PqsR Inverse Agonist Potentiates Tobramycin Efficacy to Eradicate Pseudomonas aeruginosa Biofilms

Pseudomonas aeruginosa (PA) infections can be notoriously difficult to treat and are often accompanied by the development of antimicrobial resistance (AMR). Quorum sensing inhibitors (QSI) acting on PqsR (MvfR) – a crucial transcriptional regulator serving major functions in PA virulence – can enhance antibiotic efficacy and eventually prevent the AMR. An integrated drug discovery campaign including design, medicinal chemistry-driven hit-to-lead optimization and in-depth biological profiling of a new QSI generation is reported. The QSI possess excellent activity in inhibiting pyocyanin production and PqsR reporter-gene with IC50 values as low as 200 and 11 × 10−9 m, respectively. Drug metabolism and pharmacokinetics (DMPK) as well as safety pharmacology studies especially highlight the promising translational properties of the lead QSI for pulmonary applications. Moreover, target engagement of the lead QSI is shown in a PA mucoid lung infection mouse model. Beyond that, a significant synergistic effect of a QSI-tobramycin (Tob) combination against PA biofilms using a tailor-made squalene-derived nanoparticle (NP) formulation, which enhance the minimum biofilm eradicating concentration (MBEC) of Tob more than 32-fold is demonstrated. The novel lead QSI and the accompanying NP formulation highlight the potential of adjunctive pathoblocker-mediated therapy against PA infections opening up avenues for preclinical development

Introduction of SARS in France, March–April, 2003

We describe severe acute respiratory syndrome (SARS) in France. Patients meeting the World Health Organization definition of a suspected case underwent a clinical, radiologic, and biologic assessment at the closest university-affiliated infectious disease ward. Suspected cases were immediately reported to the Institut de Veille Sanitaire. Probable case-patients were isolated, their contacts quarantined at home, and were followed for 10 days after exposure. Five probable cases occurred from March through April 2003; four were confirmed as SARS coronavirus by reverse transcription–polymerase chain reaction, serologic testing, or both. The index case-patient (patient A), who had worked in the French hospital of Hanoi, Vietnam, was the most probable source of transmission for the three other confirmed cases; two had been exposed to patient A while on the Hanoi-Paris flight of March 22–23. Timely detection, isolation of probable cases, and quarantine of their contacts appear to have been effective in preventing the secondary spread of SARS in France