Winning and Losing

There will always be winners and losers, but the focus should be on the process and not the outcome. Posting about character development through sports from In All Things - an online hub committed to the claim that the life, death, and resurrection of Jesus Christ has implications for the entire world. http://inallthings.org/winning-and-losing

What Is It Like Being a Dad?

One size parenting does not fit all children. Posting about one father\u27s experience raising kids from In All Things - an online hub committed to the claim that the life, death, and resurrection of Jesus Christ has implications for the entire world. http://inallthings.org/what-is-it-like-being-a-dad

Reformed and Biblical Counseling

What is the difference between Biblical counseling and Christian counseling? In Reformed circles, is Biblical Counseling the norm or are people encouraged to seek professional counseling help? Posting about reformed and Biblical counseling from In All Things - an online hub committed to the claim that the life, death, and resurrection of Jesus Christ has implications for the entire world. http://inallthings.org/answering-your-question-reformed-and-biblical-counseling

God, Human Nature, and Psychology

God created humans to think, act, and feel and to be in relationship with other humans. Posting about exploring the many realms of human expression­­­­­­­­ from In All Things - an online hub committed to the claim that the life, death, and resurrection of Jesus Christ has implications for the entire world. http://inallthings.org/god-human-nature-and-psychology

Mower Sharpness and Creeping Bentgrass Quality of Cut

Regular mower maintenance is essential to achieve the best possible quality of cut. Dull mowers tear leaf tissue, severely wounding the plant and resulting in formation of frayed and necrotic leaf tips. Severe wounding may limit growth and development of grasses and increase susceptibility to stresses such as drought, pathogens, insects, and traffic. The objective of this study was to quantify mower sharpness and mowing injury over time. This information will be used to develop general mower maintenance guidelines and to determine how frequently reel-type mowers should be sharpened to achieve the best possible quality of cut

Shining a Light on Mental Health

Each profession faces unique situations when it comes to serving those needing support with mental health, and it is important as Christians that we come together with a holistic approach. Posting about care for individuals with mental health crises in the various professions from In All Things - an online journal for critical reflection on faith, culture, art, and every ordinary-yet-graced square inch of God’s creation. https://inallthings.org/shining-a-light-on-mental-health

Diversification of the Caenorhabditis heat shock response by Helitron transposable elements.

Heat Shock Factor 1 (HSF-1) is a key regulator of the heat shock response (HSR). Upon heat shock, HSF-1 binds well-conserved motifs, called Heat Shock Elements (HSEs), and drives expression of genes important for cellular protection during this stress. Remarkably, we found that substantial numbers of HSEs in multiple Caenorhabditis species reside within Helitrons, a type of DNA transposon. Consistent with Helitron-embedded HSEs being functional, upon heat shock they display increased HSF-1 and RNA polymerase II occupancy and up-regulation of nearby genes in C. elegans. Interestingly, we found that different genes appear to be incorporated into the HSR by species-specific Helitron insertions in C. elegans and C. briggsae and by strain-specific insertions among different wild isolates of C. elegans. Our studies uncover previously unidentified targets of HSF-1 and show that Helitron insertions are responsible for rewiring and diversifying the Caenorhabditis HSR

Metabolomics of Oxidative Stress in Recent Studies of Endogenous and Exogenously Administered Intermediate Metabolites

Aerobic metabolism occurs in a background of oxygen radicals and reactive oxygen species (ROS) that originate from the incomplete reduction of molecular oxygen in electron transfer reactions. The essential role of aerobic metabolism, the generation and consumption of ATP and other high energy phosphates, sustains a balance of approximately 3000 essential human metabolites that serve not only as nutrients, but also as antioxidants, neurotransmitters, osmolytes, and participants in ligand-based and other cellular signaling. In hypoxia, ischemia, and oxidative stress, where pathological circumstances cause oxygen radicals to form at a rate greater than is possible for their consumption, changes in the composition of metabolite ensembles, or metabolomes, can be associated with physiological changes. Metabolomics and metabonomics are a scientific disciplines that focuse on quantifying dynamic metabolome responses, using multivariate analytical approaches derived from methods within genomics, a discipline that consolidated innovative analysis techniques for situations where the number of biomarkers (metabolites in our case) greatly exceeds the number of subjects. This review focuses on the behavior of cytosolic, mitochondrial, and redox metabolites in ameliorating or exacerbating oxidative stress. After reviewing work regarding a small number of metabolites—pyruvate, ethyl pyruvate, and fructose-1,6-bisphosphate—whose exogenous administration was found to ameliorate oxidative stress, a subsequent section reviews basic multivariate statistical methods common in metabolomics research, and their application in human and preclinical studies emphasizing oxidative stress. Particular attention is paid to new NMR spectroscopy methods in metabolomics and metabonomics. Because complex relationships connect oxidative stress to so many physiological processes, studies from different disciplines were reviewed. All, however, shared the common goal of ultimately developing “omics”-based, diagnostic tests to help influence therapies