626 research outputs found
Overexpression of a Medicago truncatula stress-associated protein gene (MtSAP1) leads to nitric oxide accumulation and confers osmotic and salt stress tolerance in transgenic tobacco
The impact of Medicago truncatula stress-associated protein gene (MtSAP1) overexpression has been investigated in Nicotiana tabacum transgenic seedlings. Under optimal conditions, transgenic lines overexpressing MtSAP1 revealed better plant development and higher chlorophyll content as compared to wild type seedlings. Interestingly, transgenic lines showed a stronger accumulation of nitric oxide (NO), a signaling molecule involved in growth and development processes. This NO production seemed to be partially nitrate reductase dependent. Due to the fact that NO has been also reported to play a role in tolerance acquisition of plants to abiotic stresses, the responses of MtSAP1 overexpressors to osmotic and salt stress have been studied. Compared to the wild type, transgenic lines were less affected in their growth and development. Moreover, NO content in MtSAP1 overexpressors was always higher than that detected in wild seedlings under stress conditions. It seems that this better tolerance induced by MtSAP1 overexpression could be associated with this higher NO production that would enable seedlings to reach a high protection level to prepare them to cope with abiotic stresses
Symmetry of Magnetically Ordered Quasicrystals
The notion of magnetic symmetry is reexamined in light of the recent
observation of long range magnetic order in icosahedral quasicrystals [Charrier
et al., Phys. Rev. Lett. 78, 4637 (1997)]. The relation between the symmetry of
a magnetically-ordered (periodic or quasiperiodic) crystal, given in terms of a
``spin space group,'' and its neutron diffraction diagram is established. In
doing so, an outline of a symmetry classification scheme for magnetically
ordered quasiperiodic crystals is provided. Predictions are given for the
expected diffraction patterns of magnetically ordered icosahedral crystals,
provided their symmetry is well described by icosahedral spin space groups.Comment: 5 pages. Accepted for publication in Phys. Rev. Letter
A hierarchy of models related to nanoflows and surface diffusion
In last years a great interest was brought to molecular transport problems at
nanoscales, such as surface diffusion or molecular flows in nano or
sub-nano-channels. In a series of papers V. D. Borman, S. Y. Krylov, A. V.
Prosyanov and J. J. M. Beenakker proposed to use kinetic theory in order to
analyze the mechanisms that determine mobility of molecules in nanoscale
channels. This approach proved to be remarkably useful to give new insight on
these issues, such as density dependence of the diffusion coefficient. In this
paper we revisit these works to derive the kinetic and diffusion models
introduced by V. D. Borman, S. Y. Krylov, A. V. Prosyanov and J. J. M.
Beenakker by using classical tools of kinetic theory such as scaling and
systematic asymptotic analysis. Some results are extended to less restrictive
hypothesis
Electrically Driven Light Emission from Individual CdSe Nanowires
We report electroluminescence (EL) measurements carried out on three-terminal
devices incorporating individual n-type CdSe nanowires. Simultaneous optical
and electrical measurements reveal that EL occurs near the contact between the
nanowire and a positively biased electrode or drain. The surface potential
profile, obtained by using Kelvin probe microscopy, shows an abrupt potential
drop near the position of the EL spot, while the band profile obtained from
scanning photocurrent microscopy indicates the existence of an n-type Schottky
barrier at the interface. These observations indicate that light emission
occurs through a hole leakage or an inelastic scattering induced by the rapid
potential drop at the nanowire-electrode interface.Comment: 12 pages, 4 figure
Radio emission of extensive air shower at CODALEMA: Polarization of the radio emission along the v*B vector
Cosmic rays extensive air showers (EAS) are associated with transient radio
emission, which could provide an efficient new detection method of high energy
cosmic rays, combining a calorimetric measurement with a high duty cycle. The
CODALEMA experiment, installed at the Radio Observatory in Nancay, France, is
investigating this phenomenon in the 10^17 eV region. One challenging point is
the understanding of the radio emission mechanism. A first observation
indicating a linear relation between the electric field produced and the cross
product of the shower axis with the geomagnetic field direction has been
presented (B. Revenu, this conference). We will present here other strong
evidences for this linear relationship, and some hints on its physical origin.Comment: Contribution to the 31st International Cosmic Ray Conference, Lodz,
Poland, July 2009. 4 pages, 8 figures. v2: Typo fixed, arxiv references adde
A random cell motility gradient downstream of FGF controls elongation of amniote embryos
Vertebrate embryos are characterized by an elongated antero-posterior (AP) body axis, which forms by progressive cell deposition from a posterior growth zone in the embryo. Here, we used tissue ablation in the chicken embryo to demonstrate that the caudal presomitic mesoderm (PSM) has a key role in axis elongation. Using time-lapse microscopy, we analysed the movements of fluorescently labelled cells in the PSM during embryo elongation, which revealed a clear posterior-to-anterior gradient of cell motility and directionality in the PSM. We tracked the movement of the PSM extracellular matrix in parallel with the labelled cells and subtracted the extracellular matrix movement from the global motion of cells. After subtraction, cell motility remained graded but lacked directionality, indicating that the posterior cell movements associated with axis elongation in the PSM are not intrinsic but reflect tissue deformation. The gradient of cell motion along the PSM parallels the fibroblast growth factor (FGF)/mitogen-activated protein kinase (MAPK) gradient1, which has been implicated in the control of cell motility in this tissue2. Both FGF signalling gain- and loss-of-function experiments lead to disruption of the motility gradient and a slowing down of axis elongation. Furthermore, embryos treated with cell movement inhibitors (blebbistatin or RhoK inhibitor), but not cell cycle inhibitors, show a slower axis elongation rate. We propose that the gradient of random cell motility downstream of FGF signalling in the PSM controls posterior elongation in the amniote embryo. Our data indicate that tissue elongation is an emergent property that arises from the collective regulation of graded, random cell motion rather than by the regulation of directionality of individual cellular movements
Expression and trans-specific polymorphism of self-incompatibility RNases in Coffea (Rubiaceae)
Self-incompatibility (SI) is widespread in the angiosperms, but identifying the biochemical components of SI mechanisms has proven to be difficult in most lineages. Coffea (coffee; Rubiaceae) is a genus of old-world tropical understory trees in which the vast majority of diploid species utilize a mechanism of gametophytic self-incompatibility (GSI). The S-RNase GSI system was one of the first SI mechanisms to be biochemically characterized, and likely represents the ancestral Eudicot condition as evidenced by its functional characterization in both asterid (Solanaceae, Plantaginaceae) and rosid (Rosaceae) lineages. The S-RNase GSI mechanism employs the activity of class III RNase T2 proteins to terminate the growth of "self" pollen tubes. Here, we investigate the mechanism of Coffea GSI and specifically examine the potential for homology to S-RNase GSI by sequencing class III RNase T2 genes in populations of 14 African and Madagascan Coffea species and the closely related self-compatible species Psilanthus ebracteolatus. Phylogenetic analyses of these sequences aligned to a diverse sample of plant RNase T2 genes show that the Coffea genome contains at least three class III RNase T2 genes. Patterns of tissue-specific gene expression identify one of these RNase T2 genes as the putative Coffea S-RNase gene. We show that populations of SI Coffea are remarkably polymorphic for putative S-RNase alleles, and exhibit a persistent pattern of trans-specific polymorphism characteristic of all S-RNase genes previously isolated from GSI Eudicot lineages. We thus conclude that Coffea GSI is most likely homologous to the classic Eudicot S-RNase system, which was retained since the divergence of the Rubiaceae lineage from an ancient SI Eudicot ancestor, nearly 90 million years ago.United States National Science Foundation [0849186]; Society of Systematic Biologists; American Society of Plant Taxonomists; Duke University Graduate Schoolinfo:eu-repo/semantics/publishedVersio
CRMP5 Regulates Generation and Survival of Newborn Neurons in Olfactory and Hippocampal Neurogenic Areas of the Adult Mouse Brain
The Collapsin Response Mediator Proteins (CRMPs) are highly expressed in the developing brain, and in adult brain areas that retain neurogenesis, ie: the olfactory bulb (OB) and the dentate gyrus (DG). During brain development, CRMPs are essentially involved in signaling of axon guidance and neurite outgrowth, but their functions in the adult brain remain largely unknown. CRMP5 has been initially identified as the target of auto-antibodies involved in paraneoplasic neurological diseases and further implicated in a neurite outgrowth inhibition mediated by tubulin binding. Interestingly, CRMP5 is also highly expressed in adult brain neurogenic areas where its functions have not yet been elucidated. Here we observed in both neurogenic areas of the adult mouse brain that CRMP5 was present in proliferating and post-mitotic neuroblasts, while they migrate and differentiate into mature neurons. In CRMP5−/− mice, the lack of CRMP5 resulted in a significant increase of proliferation and neurogenesis, but also in an excess of apoptotic death of granule cells in the OB and DG. These findings provide the first evidence that CRMP5 is involved in the generation and survival of newly generated neurons in areas of the adult brain with a high level of activity-dependent neuronal plasticity
Perception of Male Caller Identity in Koalas (Phascolarctos cinereus): Acoustic Analysis and Playback Experiments
The ability to signal individual identity using vocal signals and distinguish between conspecifics based on vocal cues is important in several mammal species. Furthermore, it can be important for receivers to differentiate between callers in reproductive contexts. In this study, we used acoustic analyses to determine whether male koala bellows are individually distinctive and to investigate the relative importance of different acoustic features for coding individuality. We then used a habituation-discrimination paradigm to investigate whether koalas discriminate between the bellow vocalisations of different male callers. Our results show that male koala bellows are highly individualized, and indicate that cues related to vocal tract filtering contribute the most to vocal identity. In addition, we found that male and female koalas habituated to the bellows of a specific male showed a significant dishabituation when they were presented with bellows from a novel male. The significant reduction in behavioural response to a final rehabituation playback shows this was not a chance rebound in response levels. Our findings indicate that male koala bellows are highly individually distinctive and that the identity of male callers is functionally relevant to male and female koalas during the breeding season. We go on to discuss the biological relevance of signalling identity in this species' sexual communication and the potential practical implications of our findings for acoustic monitoring of male population levels
Geomagnetic origin of the radio emission from cosmic ray induced air showers observed by CODALEMA
The new setup of the CODALEMA experiment installed at the Radio Observatory
in Nancay, France, is described. It includes broadband active dipole antennas
and an extended and upgraded particle detector array. The latter gives access
to the air shower energy, allowing us to compute the efficiency of the radio
array as a function of energy. We also observe a large asymmetry in counting
rates between showers coming from the North and the South in spite of the
symmetry of the detector. The observed asymmetry can be interpreted as a
signature of the geomagnetic origin of the air shower radio emission. A simple
linear dependence of the electric field with respect to vxB is used which
reproduces the angular dependencies of the number of radio events and their
electric polarity.Comment: 9 pages, 15 figures, 1 tabl
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