Hematite (U-Th)/He Thermochronometry Detects Asperity Flash Heating During Laboratory Earthquakes

Evidence for coseismic temperature rise that induces dynamic weakening is challenging to directly observe and quantify in natural and experimental fault rocks. Hematite (U-Th)/He (hematite He) thermochronometry may serve as a fault-slip thermometer, sensitive to transient high temperatures associated with earthquakes. We test this hypothesis with hematite deformation experiments at seismic slip rates, using a rotary-shear geometry with an annular ring of silicon carbide (SiC) sliding against a specular hematite slab. Hematite is characterized before and after sliding via textural and hematite He analyses to quantify He loss over variable experimental conditions. Experiments yield slip surfaces localized in an ∼5–30-µm-thick layer of hematite gouge with71% ± 1% (1σ) and 18% ± 3% He loss, respectively. Documented He loss requires short-duration, high temperatures during slip. The spatial heterogeneity and enhanced He loss from FM zones are consistent with asperity flash heating (AFH). Asperities \u3e200–300 µm in diameter, producing temperatures \u3e900 °C for ∼1 ms, can explain observed He loss. Results provide new empirical evidence describing AFH and the role of coseismic temperature rise in FM formation. Hematite He thermochronometry can detect AFH and thus seismicity on natural FMs and other thin slip surfaces in the upper seismogenic zone of Earth’s crust

Testing single aliquot regenerative dose (SAR) protocols for violet stimulated luminescence

Basic assumptions of the single aliquot regenerative dose (SAR) protocol are tested using the violet stimulated luminescence (VSL) signal from quartz. The VSL signal is shown to be reduced to a sufficiently low background level between SAR steps, and the SAR protocol appears to adequately correct for sensitivity changes during measurement. The VSL SAR protocol can recover a large (405 Gy) laboratory beta dose within uncertainties, however the mean value for the dose recovery ratio is commonly 0.8 or less. This poor behaviour is echoed in the measurements of equivalent dose (De) for a sample with an expected De of ∼354 Gy, which underestimates De by 50–70%. Further investigations are required to understand the mechanisms underlying these underestimations in VSL SAR De values

Design of (ω-N-(O-acyl)hydroxy amid) aminodicarboxylic acid pyrrolidides as potent inhibitors of proline-specific peptidases

AbstractA novel class of competitive, acylating inhibitors for the proline-specific peptidases: dipeptidyl peptidase IV, dipeptidyl peptidase II and prolyl endopeptidase, has been developed. The inhibitor molecules combine the efficacy of aminoacyl pyrrolidides and the potential transacylating capability of diacyl hydroxyl amines. The N-terminal deblocked inhibitors are potent reversible inhibitors of porcine kidney dipeptidyl peptidase IV, human placenta dipeptidyl peptidase II exhibiting K1 values in the μM range. Boc-protected (ω-N-hydroxy acyl amid) aminodiacarboxylic acid pyrrolidides inhibit substrate hydrolysis by prolyl endopeptidases from different sources competitively reaching K, values of 30 nM to 60 μM. Additionally, α-N-BOC-(ω-N-hydroxy acetyl) glutaminyl pyrrolidide modifies human placenta prolyl endopeptidase in a time-dependent reaction

Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells

Peptidoglycans provide bacterial cell walls with mechanical strength. The spatial organization of peptidoglycan has previously been difficult to study. Here, atomic force microscopy, together with cells carrying mutations in cell-wall polysaccharides, has allowed an in-depth study of these molecules

Interventions Targeting Child Undernutrition in Developing Countries May Be Undermined by Dietary Exposure to Aflatoxin

Child undernutrition, a form of malnutrition, is a major public health burden in developing countries. Supplementation interventions targeting the major micronutrient deficiencies have only reduced the burden of child undernutrition to a certain extent, indicating that there are other underlying determinants that need to be addressed. Aflatoxin exposure, which is also highly prevalent in developing countries, may be considered an aggravating factor for child undernutrition. Increasing evidence suggests that aflatoxin exposure can occur in any stage of life, including in utero through a trans-placental pathway and in early childhood (through contaminated weaning food and family food). Early life exposure to aflatoxin is associated with adverse effects on low birth weight, stunting, immune suppression, and the liver function damage. The mechanisms underlying impaired growth and aflatoxin exposure are still unclear but intestinal function damage, reduced immune function, and alteration in the insulin-like growth factor axis caused by the liver damage are the suggested hypotheses. Given the fact that both aflatoxin and child undernutrition are common in sub-Saharan Africa, effective interventions aimed at reducing undernutrition cannot be satisfactorily achieved until the interactive relationship between aflatoxin and child undernutrition is clearly understood, and an aflatoxin mitigation strategy takes effect in those vulnerable mothers and children

The chronostratigraphy of the Haua Fteah cave (Cyrenaica, northeast Libya) — optical dating of early human occupation during Marine Isotope Stages 4, 5 and 6

The paper presents the results of optical dating of potassium-rich feldspar grains obtained from the Haua Fteah cave in Cyrenaica, northeast Libya, focussing on the chronology of the Deep Sounding excavated by Charles McBurney in the 1950s and reexcavated recently. Samples were also collected from a 1.25 m-deep trench (Trench S) excavated during the present project below the basal level of the Deep Sounding. Optically stimulated luminescence (OSL) data sets for multi-grain, single aliquots of quartz for samples from the Middle Trench were previously published. Re-analyses of these OSL data confirm significant variation in the dose saturation levels of the quartz signal, but allow the most robust OSL ages to be determined for comparison with previous age estimates and with those obtained in this study for potassium-rich feldspars from the Deep Sounding. The latter indicate that humans may have started to visit the cave as early as ~150 ka ago, but that major use of the cave occurred during MIS 5, with the accumulation of the Deep Sounding sediments. Correlations between optical ages and episodes of “Pre-Aurignacian” artefact discard indicate that human use of the cave during MIS 5 was highly intermittent. The earliest phases of human activity appear to have occurred during interstadial conditions (5e and 5c), with a later phase of lithic discard associated with more stadial conditions, possibly MIS 5b. We argue that the “Pre-Aurignacian” assemblage can probably be linked with modern humans, like the succeeding “Levalloiso-Mousterian” assemblage; two modern human mandibles associated with the latter are associated with a modelled age of 73–65 ka. If this attribution is correct, then the new chronology implies that modern humans using “Pre-Aurignacian” technologies were in Cyrenaica as early as modern humans equipped with “Aterian” technologies were in the Maghreb, raising new questions about variability among lithic technologies during the initial phases of modern human dispersals into North Africa

Synthetic Mimic of Antimicrobial Peptide with Nonmembrane-Disrupting Antibacterial Properties

Proteolysis in dairy lactic acid bacteria has been studied in great detail by genetic, biochemical and ultrastructural methods. From these studies the picture emerges that the proteolytic systems of lactococci and lactobacilli are remarkably similar in their components and mode of action. The proteolytic system consists of an extracellularly located serine-proteinase, transport systems specific for di-tripeptides and oligopeptides (> 3 residues), and a multitude of intracellular peptidases. This review describes the properties and regulation of individual components as well as studies that have led to identification of their cellular localization. Targeted mutational techniques developed in recent years have made it possible to investigate the role of individual and combinations of enzymes in vivo. Based on these results as well as in vitro studies of the enzymes and transporters, a model for the proteolytic pathway is proposed. The main features are: (i) proteinases have a broad specificity and are capable of releasing a large number of different oligopeptides, of which a large fraction falls in the range of 4 to 8 amino acid residues; (ii) oligopeptide transport is the main route for nitrogen entry into the cell; (iii) all peptidases are located intracellularly and concerted action of peptidases is required for complete degradation of accumulated peptides.