Developing Tailor-Made Microbial Consortium for Effluent Remediation

The work describes a biofilm-based soluble sulphate reduction system, which can treat up to 1600 ppm of soluble sulphate within 3.5 hours of incubation to discharge level under ambient condition using a well-characterized sulphate-reducing bacterial (SRB) consortium. This system ensures the treatment of 1509 litres of sulphate solution in 24 hours using a 220-litre bioreactor. Performance of the system during series operation was compromised, indicating the presence of inhibitor in solution at a toxic level. A single unit bioreactor would be the ideal configuration for this consortium. Modified designs of bioreactors were tested for optimization of the process using response surface methodology (RSM), where the system could function optimally at an initial sulphate concentration of 1250 ppm with a flow rate of 1.8 litre/hour. The time course of sulphate reduction yielded a parabolic profile (with coefficient of determination r 2 = 0.99 and p value < 0.05). The rate of sulphate reduction was found to be independent of seasonal variation as well as the specific design characteristic

High spin band structures in doubly-odd 194^{194}Tl

The high-spin states in odd-odd $^{194}$Tl nucleus have been studied by populating them using the $^{185,187}$Re($^{13}$C, xn) reactions at 75 MeV of beam energy. $\gamma-\gamma$ coincidence measurement has been performed using the INGA array with a digital data acquisition system to record the time stamped data. Definite spin-parity assignment of the levels was made from the DCO ratio and the IPDCO ratio measurements. The level scheme of $^{194}$Tl has been extended up to 4.1 MeV in excitation energy including 19 new gamma ray transitions. The $\pi h_{9/2} \otimes \nu i_{13/2}$ band, in the neighboring odd-odd Tl isotopes show very similar properties in both experimental observables and calculated shapes. Two new band structures, with 6-quasiparticle configuration, have been observed for the first time in $^{194}$Tl. One of these bands has the characteristics of a magnetic rotational band. The cranked shell model calculations, using a deformed Woods-Saxon potential, have been performed to obtain the total Routhian surfaces in order to study the shapes of the bands and the band crossing in $^{194}$Tl. The semiclassical formalism has been used to describe the magnetic rotational band.Comment: Accepted for publication in Physical Review

Correction of WOW and Flutter in Audio Signals using Least Mean Square Algorithm

This paper describes a system which is used to remove Wow and Flutter from audio signal using Least Mean Square Algorithm. It is impossible to completely remove Wow and Flutter from audio signal but its effect can be reduced significantly. It occurs during the process of sound reproduction. It is the group of tones created by the irregularities in turntables or tape drive speed during reproduction, duplication or recording. Wow occurs due to irregularities at low frequency whereas at high frequency irregularities, Flutter occurs. Least Mean Square Algorithm uses Adaptive Filter which adjusts their coefficient in order to minimize the required wobble effects in audio signal. Results show that LMS has comprehensively diminished the effects of wow and flutter

Mechanism of apoptosis induction in human breast cancer MCF-7 cell by Ruviprase, a small peptide from Daboia russelii russelii venom

Ruviprase, a 4.4 kDa peptide isolated from Daboia russelii russelii venom demonstrated antiproliferative activity against EMT6/AR1, U-87MG, HeLa and MCF-7 cancer cells with an IC50 value of 23.0, 8.8, 5.8 and 4.0 mu g ml(-1), respectively. However, it was nontoxic to non-cancerous human embryonic kidney cell and human peripheral blood lymphocytes. Flow-cytometric analysis confirmed the apoptosis induction in MCF-7 cells by Ruviprase where it induced DNA condensation but did not cause mitotic blockage or chromosomal aberration in treated-cells. Immunofluorescence microscopic analysis indicated Ruviprase induced apoptosis in MCF-7 cells through p53 and p21-mediated pathways. Ruviprase generated reactive oxygen species (ROS), altered the mitochondrial transmembrane potential, and significantly decreased the cellular glutathione (GSH) content of MCF-7 cells. Immunoblotting and quantitative real-time PCR (qRT-PCR) analyses suggested that Ruviprase down-regulated the expression of anti-apoptotic protein Bcl-2, increased cleavage of poly (ADP-ribose) polymerase (PARP) protein, and up-regulated the expression of pro-apoptotic protein Bax, as well as executer protein caspase-7 to induced apoptosis in MCF-7 cells via intrinsic pathway. This is the first report on the characterization of the anticancer potential of a small, non-toxic and anticoagulant peptide purified from Russell's viper venom. (C) 2016 Elsevier Ireland Ltd. All rights reserved

Localized extension in megathrust hanging wall following great earthquakes in western Nepal

International audienceThe largest (M8+) known earthquakes in the Himalaya have ruptured the upper locked section of the Main Himalayan Thrust zone, offsetting the ground surface along the Main Frontal Thrust at the range front. However, out-of-sequence active structures have received less attention. One of the most impressive examples of such faults is the active fault that generally follows the surface trace of the Main Boundary Thrust (MBT). This fault has generated a clear geomorphological signature of recent deformation in eastern and western Nepal, as well as further west in India. We focus on western Nepal, between the municipalities of Surkhet and Gorahi where this fault is well expressed. Although the fault system as a whole is accommodating contraction, across most of its length, this particular fault appears geomorphologically as a normal fault, indicating crustal extension in the hanging wall of the MHT. We focus this study on the reactivation of the MBT along the Surkhet-Gorahi segment of the surface trace of the newly named Reactivated Boundary Fault, which is ~ 120 km long. We first generate a high-resolution Digital Elevation Model from triplets of high-resolution Pleiades images and use this to map the fault scarp and its geomorphological lateral variation. For most of its length, normal motion slip is observed with a dip varying between 20° and 60° and a maximum cumulative vertical offset of 27 m. We then present evidence for recent normal faulting in a trench located in the village of Sukhetal. Radiocarbon dating of detrital charcoals sampled in the hanging wall of the fault, including the main colluvial wedge and overlying sedimentary layers, suggest that the last event occurred in the early sixteenth century. This period saw the devastating 1505 earthquake, which produced ~ 23 m of slip on the Main Frontal Thrust. Linked or not, the ruptures on the MFT and MBT happened within a short time period compared to the centuries of quiescence of the faults that followed. We suggest that episodic normal-sense activity of the MBT could be related to large earthquakes rupturing the MFT, given its proximity, the sense of motion, and the large distance that separates the MBT from the downdip end of the locked fault zone of the MHT fault system. We discuss these results and their implications for the frontal Himalayan thrust system