32 research outputs found
Papaya recombinant inbred lines selection by image-based phenotyping
The selection of superior Carica papaya (L) genotypes depends on the availability of genetic variability and on the favorable and simultaneous response of the genotypes to those traits of most interest. However, manual phenotyping (MP) demands intensive labor, is time-consuming and expensive. The aim of the current study is to access the efficiency of image-based phenotyping (IBP) in estimating genetic parameters and in selecting F4 recombinant inbred lines. The genetic parameters and values were estimated in accordance with the REML/BLUB procedure and combined selection using the selection index based on standardized genetic values. The majority of traits accessed through IBP showed experimental coefficients of variation similar to those found through MP. Both methodologies showed genetic parameters of similar magnitude, indicating expressive genetic variability between lines in the traits accessed in this study. The same superior lines were indicated in both methodologies and expressive genetic gains obtained through the lines were selected for all traits. IBP performance was similar to that of MP with respect to the estimates of breeding-relevant traits such as commercial fruits and yield. Thus, IBP showed efficient phenotypic assessment, as well as selective accuracy in accessing genetic variability and genetic gains, when it was compared to MP. Since IBP is far less dependent on labor, it is expected to be incorporated into the routine of papaya breeding programs as a way of increasing the number of accessed lines and, consequently, increasing genetic gains
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4
While the increasing availability of global databases on ecological communities has advanced our knowledge
of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In
the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of
Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus
crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced
environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian
Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by
2050. This means that unless we take immediate action, we will not be able to establish their current status,
much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio
Diretrizes Brasileiras de Medidas da Pressão Arterial Dentro e Fora do Consultório – 2023
Hypertension is one of the primary modifiable risk factors for morbidity and mortality worldwide, being a major risk factor for coronary artery disease, stroke, and kidney failure. Furthermore, it is highly prevalent, affecting more than one-third of the global population.
Blood pressure measurement is a MANDATORY procedure in any medical care setting and is carried out by various healthcare professionals. However, it is still commonly performed without the necessary technical care. Since the diagnosis relies on blood pressure measurement, it is clear how important it is to handle the techniques, methods, and equipment used in its execution with care.
It should be emphasized that once the diagnosis is made, all short-term, medium-term, and long-term investigations and treatments are based on the results of blood pressure measurement. Therefore, improper techniques and/or equipment can lead to incorrect diagnoses, either underestimating or overestimating values, resulting in inappropriate actions and significant health and economic losses for individuals and nations.
Once the correct diagnosis is made, as knowledge of the importance of proper treatment advances, with the adoption of more detailed normal values and careful treatment objectives towards achieving stricter blood pressure goals, the importance of precision in blood pressure measurement is also reinforced.
Blood pressure measurement (described below) is usually performed using the traditional method, the so-called casual or office measurement. Over time, alternatives have been added to it, through the use of semi-automatic or automatic devices by the patients themselves, in waiting rooms or outside the office, in their own homes, or in public spaces. A step further was taken with the use of semi-automatic devices equipped with memory that allow sequential measurements outside the office (ABPM; or HBPM) and other automatic devices that allow programmed measurements over longer periods (HBPM).
Some aspects of blood pressure measurement can interfere with obtaining reliable results and, consequently, cause harm in decision-making. These include the importance of using average values, the variation in blood pressure during the day, and short-term variability. These aspects have encouraged the performance of a greater number of measurements in various situations, and different guidelines have advocated the use of equipment that promotes these actions. Devices that perform HBPM or ABPM, which, in addition to allowing greater precision, when used together, detect white coat hypertension (WCH), masked hypertension (MH), sleep blood pressure alterations, and resistant hypertension (RHT) (defined in Chapter 2 of this guideline), are gaining more and more importance.
Taking these details into account, we must emphasize that information related to diagnosis, classification, and goal setting is still based on office blood pressure measurement, and for this reason, all attention must be given to the proper execution of this procedure.La hipertensión arterial (HTA) es uno de los principales factores de riesgo modificables para la morbilidad y mortalidad en todo el mundo, siendo uno de los mayores factores de riesgo para la enfermedad de las arterias coronarias, el accidente cerebrovascular (ACV) y la insuficiencia renal. Además, es altamente prevalente y afecta a más de un tercio de la población mundial.
La mediciĂłn de la presiĂłn arterial (PA) es un procedimiento OBLIGATORIO en cualquier atenciĂłn mĂ©dica o realizado por diferentes profesionales de la salud. Sin embargo, todavĂa se realiza comĂşnmente sin los cuidados tĂ©cnicos necesarios. Dado que el diagnĂłstico se basa en la mediciĂłn de la PA, es claro el cuidado que debe haber con las tĂ©cnicas, los mĂ©todos y los equipos utilizados en su realizaciĂłn.
Debemos enfatizar que una vez realizado el diagnĂłstico, todas las investigaciones y tratamientos a corto, mediano y largo plazo se basan en los resultados de la mediciĂłn de la PA. Por lo tanto, las tĂ©cnicas y/o equipos inadecuados pueden llevar a diagnĂłsticos incorrectos, subestimando o sobreestimando valores y resultando en conductas inadecuadas y pĂ©rdidas significativas para la salud y la economĂa de las personas y las naciones.
Una vez realizado el diagnóstico correcto, a medida que avanza el conocimiento sobre la importancia del tratamiento adecuado, con la adopción de valores de normalidad más detallados y objetivos de tratamiento más cuidadosos hacia metas de PA más estrictas, también se refuerza la importancia de la precisión en la medición de la PA.
La mediciĂłn de la PA (descrita a continuaciĂłn) generalmente se realiza mediante el mĂ©todo tradicional, la llamada mediciĂłn casual o de consultorio. Con el tiempo, se han agregado alternativas a travĂ©s del uso de dispositivos semiautomáticos o automáticos por parte del propio paciente, en salas de espera o fuera del consultorio, en su propia residencia o en espacios pĂşblicos. Se dio un paso más con el uso de dispositivos semiautomáticos equipados con memoria que permiten mediciones secuenciales fuera del consultorio (AMPA; o MRPA) y otros automáticos que permiten mediciones programadas durante perĂodos más largos (MAPA).
Algunos aspectos en la mediciĂłn de la PA pueden interferir en la obtenciĂłn de resultados confiables y, en consecuencia, causar daños en las decisiones a tomar. Estos incluyen la importancia de usar valores promedio, la variaciĂłn de la PA durante el dĂa y la variabilidad a corto plazo. Estos aspectos han alentado la realizaciĂłn de un mayor nĂşmero de mediciones en diversas situaciones, y diferentes pautas han abogado por el uso de equipos que promuevan estas acciones. Los dispositivos que realizan MRPA o MAPA, que además de permitir una mayor precisiĂłn, cuando se usan juntos, detectan la hipertensiĂłn de bata blanca (HBB), la hipertensiĂłn enmascarada (HM), las alteraciones de la PA durante el sueño y la hipertensiĂłn resistente (HR) (definida en el CapĂtulo 2 de esta guĂa), están ganando cada vez más importancia.
Teniendo en cuenta estos detalles, debemos enfatizar que la informaciĂłn relacionada con el diagnĂłstico, la clasificaciĂłn y el establecimiento de objetivos todavĂa se basa en la mediciĂłn de la presiĂłn arterial en el consultorio, y por esta razĂłn, se debe prestar toda la atenciĂłn a la ejecuciĂłn adecuada de este procedimiento.A hipertensĂŁo arterial (HA) Ă© um dos principais fatores de risco modificáveis para morbidade e mortalidade em todo o mundo, sendo um dos maiores fatores de risco para doença arterial coronária, acidente vascular cerebral (AVC) e insuficiĂŞncia renal. AlĂ©m disso, Ă© altamente prevalente e atinge mais de um terço da população mundial.
A medida da PA é procedimento OBRIGATÓRIO em qualquer atendimento médico ou realizado por diferentes profissionais de saúde. Contudo, ainda é comumente realizada sem os cuidados técnicos necessários. Como o diagnóstico se baseia na medida da PA, fica claro o cuidado que deve haver com as técnicas, os métodos e os equipamentos utilizados na sua realização.
Deve-se reforçar que, feito o diagnĂłstico, toda a investigação e os tratamentos de curto, mĂ©dio e longo prazos sĂŁo feitos com base nos resultados da medida da PA. Assim, tĂ©cnicas e/ou equipamentos inadequados podem levar a diagnĂłsticos incorretos, tanto subestimando quanto superestimando valores e levando a condutas inadequadas e grandes prejuĂzos Ă saĂşde e Ă economia das pessoas e das nações.
Uma vez feito o diagnóstico correto, na medida em que avança o conhecimento da importância do tratamento adequado, com a adoção de valores de normalidade mais detalhados e com objetivos de tratamento mais cuidadosos no sentido do alcance de metas de PA mais rigorosas, fica também reforçada a importância da precisão na medida da PA.
A medida da PA (descrita a seguir) Ă© habitualmente feita pelo mĂ©todo tradicional, a assim chamada medida casual ou de consultĂłrio. Ao longo do tempo, foram agregadas alternativas a ela, mediante o uso de equipamentos semiautomáticos ou automáticos pelo prĂłprio paciente, nas salas de espera ou fora do consultĂłrio, em sua prĂłpria residĂŞncia ou em espaços pĂşblicos. Um passo adiante foi dado com o uso de equipamentos semiautomáticos providos de memĂłria que permitem medidas sequenciais fora do consultĂłrio (AMPA; ou MRPA) e outros automáticos que permitem medidas programadas por perĂodos mais prolongados (MAPA).
Alguns aspectos na medida da PA podem interferir na obtenção de resultados fidedignos e, consequentemente, causar prejuĂzo nas condutas a serem tomadas. Entre eles, estĂŁo: a importância de serem utilizados valores mĂ©dios, a variação da PA durante o dia e a variabilidade a curto prazo. Esses aspectos tĂŞm estimulado a realização de maior nĂşmero de medidas em diversas situações, e as diferentes diretrizes tĂŞm preconizado o uso de equipamentos que favoreçam essas ações. Ganham cada vez mais espaço os equipamentos que realizam MRPA ou MAPA, que, alĂ©m de permitirem maior precisĂŁo, se empregados em conjunto, detectam a HA do avental branco (HAB), HA mascarada (HM), alterações da PA no sono e HA resistente (HAR) (definidos no CapĂtulo 2 desta diretriz).
Resguardados esses detalhes, devemos ressaltar que as informações relacionadas a diagnóstico, classificação e estabelecimento de metas ainda são baseadas na medida da PA de consultório e, por esse motivo, toda a atenção deve ser dada à realização desse procedimento
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost
Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study
Summary
Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally.
Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies
have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of
the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income
countries globally, and identified factors associated with mortality.
Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to
hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis,
exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a
minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical
status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary
intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause,
in-hospital mortality for all conditions combined and each condition individually, stratified by country income status.
We did a complete case analysis.
Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital
diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal
malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome
countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male.
Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3).
Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income
countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups).
Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome
countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries;
p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients
combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11],
p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20
[1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention
(ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety
checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed
(ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of
parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65
[0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality.
Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome,
middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will
be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger
than 5 years by 2030
An approach to mitigate denial of service attacks in ieee 802.11 networks
Wireless networks are widely deployed and have many uses, for example in critical embedded systems. The applications of this kind of network meets the common needs of most embedded systems and addressing the particularities of each scenario, such as limitations of computing resources and energy supply. Problems such as denial of service attacks are common place and cause great inconvenience. Thus, this study presents simulations of denial of service attacks on 802.11 wireless networks using the network simulator OMNeT++. Furthermore, we present an approach to mitigate such attack, obtaining significant results for improving wireless networks
Use of Balanced Scorecard (BSC) Performance Indicators for Small-Scale Hydropower Project Attractiveness Analysis
Small-scale hydropower plants (SHP) have been losing competitiveness compared to other renewable sources in the global electricity matrix due to technical, environmental, regulatory, and economic issues. In this context, and to contribute to the development and strengthening of SHP’s integration into the country’s electricity matrix, this study proposes the Balanced Scorecard (BSC) as a strategic evaluation tool for SHP projects from the prospecting phase to the pre-implementation phase, taking into account financial and non-financial performance indicators. This study aims to structure a strategic map of the SHP source composed of economic, socio-environmental, institutional, and technical perspectives, adapted to the four perspectives of the BSC, with the required performance indicators for the strategic evaluation of SHP. The development of a computer program allowed data visualization from eight case studies focusing on indicators or perspectives that jeopardize the project’s strategic viability. The results showed that (i) the adaptation of the BSC methodology for the strategic evaluation of SHP feasibility proved effective and (ii) the developed computer program demonstrated robustness and effectiveness in analyzing SHP, even in different stages of development and with different technical and environmental characteristics. Finally, the strategic evaluation of SHP feasibility is an effective tool in assisting decision makers, indicating the positive aspects and weaknesses of the project under evaluation and providing a better understanding of the asset and the areas of development that deserve greater attention to improve its performance