Dissecting a vegetable landrace: Components of variation in Spanish ?Moruno? tomatoes as a case studio

The variability of landraces is dissected and related to farmers' practices through surveys. Spanish 'Moruno' tomatoes, recognised for their excellent flavour, were selected as a case study. For this purpose, 30 populations were characterised in a four-year program. Higher intra-population than inter-population variability was found. Variability is generally reduced in the traits used by farmers as selection criteria. Farmers rarely used flavour as a selection criterion, but it is one of the main characteristics used by them to define the landrace. Seed exchange and growing different landraces simultaneously are commonplace, and outcrossing might occur, thus justifying in part the seed degeneration problems experienced by some farmers. At the same time, farmers select seeds for the next year on a per-fruit basis rather than a per-plant basis, justifying the maintenance of a high level of variability. In such cases, high pressure would be applied to key morphological traits but not to flavour. Accordingly, the sugars, acids and volatiles profiles related to flavour in the landraces' populations are highly variable, though the environment also exercises a high effect. It is necessary to make population selections to offer farmers materials combining the best organoleptic perception and a high stable yield. This would reduce the gap between the price premium received by farmers and the expected fair price. It would also be necessary to develop consumer information campaigns to exploit their willingness to pay for the extra value offered by landraces. Only then long-term on-farm conservation would be economically feasible. Although achieving a trade-off between yield and flavour is difficult, it is possible to identify populations that reach a compromise between them. In germplasm banks, it is impossible to evaluate all the materials in the same year. The use of hybrid controls, with no genetic variation, is helpful in considering the environmental effects. Still, genotype x environment interactions are evident, and using selected control populations of landraces is necessary to evaluate possible performances closer to the type of materials being evaluated

Checklist de Festuca L. (Poaceae) en la Península Ibérica

Se ha elaborado una checklist actualizada del género Festuca en la Pe¬nínsula Ibérica. El catálogo incluye 73 especies, que suman 98 taxones si se consideran las subespecies y variedades, de los que casi el 40% son endémicos del territorio. Para cada taxón se recoge el nombre válido y las sinonimias más importantes, la indicación locotípica, el número cromosómico, las características ecológicas, la distribución general y en el territorio, y la información bibliográfica de interés. Además, se recogen en varios apéndices los nombres sin asignación, los híbridos interespecíficos detectados en el territorio, los híbridos intergenéricos conocidos, los taxones excluidos provisionamente y aquellos cuya presencia es probable en el territorio.Checklist of Festuca L. (Poaceae) in the Iberian Peninsula. We present an updated checklist of the genus Festuca in the Iberian Peninsula. The catalogue includes 73 species, suming up 98 taxa considering infraspecific taxa (subspecies and varieties). Of them, approximately 40% are endemic to the region. Information about the valid name and the most important synonyms, the locus classicus reference, the chromosome number, the ecology, the global and regional geographical distributions, and the most relevant bibliographic references is provided for each taxon. Additional information on the taxa without clear taxonomic assignation, the interspecific hybrids detected in the territory, the described intergeneric hybrids, and the taxa provisionally excluded from the Iberian checklist and those that could be present in the Iberian Peninsula is indicated in the appendices

Body awareness and mindfulness: Validation of the Spanish version of the Scale of Body Connection

BACKGROUND: To assess the psychometric properties and the factor structure of the Spanish version of the Scale of Body Connection (SBC) in a community population of meditators and non-meditators and to investigate the relationships among mindfulness, body awareness and body dissociation. METHODS: Design. Validation study. Sampling. An internet-based commercial system was used to recruit the sample. Instruments. In addition to the SBC, the Five Facet Mindfulness Questionnaire (FFMQ) and the Depression, Anxiety, and Stress Scale (DASS-21) were administered. RESULTS: A sample of 578 subjects responded to all of the items in the protocol. A total of 55.2% of respondents had some previous experience with meditation. A Scree plot showed a two-factor solution involving the Body Awareness (BA) and Body Dissociation (BD) subscales. This study differed from the original validation study in the lack of independence of the subscales; they were correlated in the present study (r=-.11). Internal consistency for BA was α: .86, and for BD, the α was .62. Test-retest reliability was assessed in a subsample (N=67) and was r=.679 for BA and r=.765 for BD. Hierarchical multiple regression analyses showed that mindfulness practice and the FFMQ factors of Observing and Describing were positive predictors of BA. Describing, Acting with awareness and Non-judging negatively predicted BD, and Observing positively predicted BD. CONCLUSION: The study confirms the adequacy of the psychometric properties of the Spanish version of the SBC for use in community samples. The relationship between SBC and mindfulness is discussed in light of previous research

Relationship between meditative practice and self-reported mindfulness: The MINDSENS composite index

Mindfulness has been described as an inherent human capability that can be learned and trained, and its improvement has been associated with better health outcomes in both medicine and psychology. Although the role of practice is central to most mindfulness programs, practice-related improvements in mindfulness skills is not consistently reported and little is known about how the characteristics of meditative practice affect different components of mindfulness. The present study explores the role of practice parameters on self-reported mindfulness skills. A total of 670 voluntary participants with and without previous meditation experience (n = 384 and n = 286, respectively) responded to an internet-based survey on various aspects of their meditative practice (type of meditation, length of session, frequency, and lifetime practice). Participants also completed the Five Facets Mindfulness Questionnaire (FFMQ), and the Experiences Questionnaire (EQ). The group with meditation experience obtained significantly higher scores on all facets of FFMQ and EQ questionnaires compared to the group without experience. However different effect sizes were observed, with stronger effects for the Observing and Non-Reactivity facets of the FFMQ, moderate effects for Decentering in EQ, and a weak effect for Non-judging, Describing, and Acting with awareness on the FFMQ. Our results indicate that not all practice variables are equally relevant in terms of developing mindfulness skills. Frequency and lifetime practice - but not session length or meditation type - were associated with higher mindfulness skills. Given that these 6 mindfulness aspects show variable sensitivity to practice, we created a composite index (MINDSENS) consisting of those items from FFMQ and EQ that showed the strongest response to practice. The MINDSENS index was able to correctly discriminate daily meditators from non-meditators in 82.3% of cases. These findings may contribute to the understanding of the development of mindfulness skills and support trainers and researchers in improving mindfulness-oriented practices and programs

Energy shortage failure prediction in photovoltaic standalone installations by using machine learning techniques

The use of energy storage systems in standalone photovoltaic installations is essential to supply energy demands, independently of solar generation. Accurate prediction of the battery state is critical for the safe, durable, and reliable operation of systems in this type of installations. In this study, an installation located in the area of Aragon (Spain) has been considered. Two methods, based on different types of Recurrent Neural Networks (RNN), are proposed to predict the battery voltage of the installation two days ahead. Specifically, the Nonlinear Auto Regressive with Exogenous Input (NARX) network and the Long Short-Term Memory (LSTM) network are studied and compared. The implemented algorithms process battery voltage, temperature and current waveforms; and rely on the selection of different future scenarios based on weather forecasting to estimate the future voltage of the battery. The proposed methodology is capable of predicting the voltage with a Root Mean Squared Error (RMSE) error of 1.2 V for batteries of 48 V, in critical situations where the installation is running out of energy. The study contributes to the ongoing research of developing preventive control systems that help reduce costs and improve the performance of remote energy storage systems based on renewable energies with a positive outcome

Analysis of Li-ion battery degradation using self-organizing maps

This paper proposes a new methodology to identify the different degradation processes of Li-Ion battery cells. The goal of this study is to determine if different degradation factors can be separated by waveform analysis from aged cells with similar remaining capacity. In contrast to other works, the proposed method identifies the past operating conditions in the cell, regardless of the actual State of Health. The methodology is based on a data-driven approach by using a SOM (Self-organizing map), an unsupervised neural network. To verify the hypothesis a SOM has been trained with laboratory data from whole data cycles, to classify cells concerning their degradation path and according to their discharge voltage patterns. Additionally, this new methodology based on the SOM allows discriminating groups of cells with different cycling conditions (based on depth of discharge, ambient temperature and discharge current). This research line is very promising for classification of used cells, not only depending on their current static parameters (capacity, impedance), but also the battery use in their past life. This will allow making predictions of the Remaining Useful Life (RUL) of a battery with greater precision

Genetic relationships within and among Iberian fescues (Festuca L.) based on PCR-amplified markers

The genus Festuca comprises approximately 450 species and is widely distributed around the world. The Iberian Penninsula, with more than 100 taxa colonizing very diverse habitats, is one of its main centers of diversification. This study was conducted to assess molecular genetic variation and genetic relatedness among 91 populations of 31 taxa of Iberian fescues, based on several molecular markers (random amplified polymorphic DNA, amplified fragment length polymorphisms, and trnL sequences). The analyses showed the paraphyletic origin of the broad-leaved (subgenus Festuca, sections Scariosae and Subbulbosae, and subgenus Schedonorus) and the fine-leaved fescues (subgenus Festuca, sections Aulaxyper, Eskia, and Festuca). Schedonorus showed a weak relationship with Lolium rigidum and appeared to be the most recent of the broad-leaved clade. Section Eskia was the most ancient and Festuca the most recent of the fine-leaved clade. Festuca and Aulaxyper were the most related sections, in concordance with their taxonomic affinities. All taxa grouped into their sections, except F. ampla and F. capillifolia (section Festuca), which appeared to be more closely related to Aulaxyper and to a new independent section, respectively. Most populations clustered at the species level, but some subspecies and varieties mixed their populations. This study demonstrated the value in combining different molecular markers to uncover hidden genetic relationships between populations of Festuca

Grafting Snake Melon [Cucumis melo L. subsp. melo Var. flexuosus (L.) Naudin] in Organic Farming: Effects on Agronomic Performance; Resistance to Pathogens; Sugar, Acid, and VOC Profiles; and Consumer Acceptance

The performance of snake melon [Cucumis melo var. flexuosus (L.)] in organic farming was studied under high biotic and salt stress conditions. Soilborne diseases (mainly caused by Macrophomina phaseolina and Neocosmospora falciformis), combined with virus incidence [Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV), and Tomato leaf curl New Delhi virus (ToLCNDV)] and Podosphaera xanthii attacks, reduced yield by more than 50%. Snake melon susceptibility to M. phaseolina and Monosporascus cannonballus was proved in pathogenicity tests, while it showed some degree of resistance to Neocosmospora keratoplastica and N. falciformis. On the contrary, salt stress had a minor impact, although a synergic effect was detected: yield losses caused by biotic stress increased dramatically when combined with salt stress. Under biotic stress, grafting onto the melon F1Pat81 and wild Cucumis rootstocks consistently reduced plant mortality in different agroecological conditions, with a better performance compared to classic Cucurbita commercial hybrids. Yield was even improved under saline conditions in grafted plants. A negative effect was detected, though, on consumer acceptability, especially with the use of Cucurbita rootstocks. Cucumis F1Pat81 rootstock minimized this side effect, which was probably related to changes in the profile of sugars, acids, and volatiles. Grafting affected sugars and organic acid contents, with this effect being more accentuated with the use of Cucurbita rootstocks than with Cucumis. In fact, the latter had a higher impact on the volatile organic compound profile than on sugar and acid profile, which may have resulted in a lower effect on consumer perception. The use of Cucumis rootstocks seems to be a strategy to enable organic farming production of snake melon targeted to high-quality markets in order to promote the cultivation of this neglected crop.

Toward the Assessment of Food Toxicity for Celiac Patients: Characterization of Monoclonal Antibodies to a Main Immunogenic Gluten Peptide

13 pages, 8 figures.-- PMID: 18509534 [PubMed].-- PMCID: PMC2386552.[Background and Aims] Celiac disease is a permanent intolerance to gluten prolamins from wheat, barley, rye and, in some patients, oats. Partially digested gluten peptides produced in the digestive tract cause inflammation of the small intestine. High throughput, immune-based assays using monoclonal antibodies specific for these immunotoxic peptides would facilitate their detection in food and enable monitoring of their enzymatic detoxification. Two monoclonal antibodies, G12 and A1, were developed against a highly immunotoxic 33-mer peptide. The potential of each antibody for quantifying food toxicity for celiac patients was studied.[Methods] Epitope preferences of G12 and A1 antibodies were determined by ELISA with gluten-derived peptide variants of recombinant, synthetic or enzymatic origin.[Results] The recognition sequences of G12 and A1 antibodies were hexameric and heptameric epitopes, respectively. Although G12 affinity for the 33-mer was superior to A1, the sensitivity for gluten detection was higher for A1. This observation correlated to the higher number of A1 epitopes found in prolamins than G12 epitopes. Activation of T cell from gluten digested by glutenases decreased equivalently to the detection of intact peptides by A1 antibody. Peptide recognition of A1 included gliadin peptides involved in the both the adaptive and innate immunological response in celiac disease.[Conclusions] The sensitivity and epitope preferences of the A1 antibody resulted to be useful to detect gluten relevant peptides to infer the potential toxicity of food for celiac patients as well as to monitor peptide modifications by transglutaminase 2 or glutenases.This work was supported by the Asociación de Celiacos de Madrid (to Carolina Sousa), by the CTA (Corporación Tecnológica de Andalucía) and IDEA (Agencia de Innovación y Desarrollo de Andalucía) (to Angel Cebolla) and by grants BFU2007-64999 from Plan Nacional de Investigación científica, Desarrollo e Innovación tecnológica (Miniterio de Educación y Ciencia) and RICET-RD06/0021-0014, Spain (to Manuel C. López). Belén Morón was supported by a fellowship from Consejo Andaluz de Colegios Oficiales de Farmacéuticos.Peer reviewe