a microanalytical case study

UIDB/00693/2020 UIDP/00693/2020The literature reports the benefits of multimodal interaction with the maternal voice for preterm dyads in kangaroo care. Little is known about multimodal interaction and vocal modulation between preterm mother–twin dyads. This study aims to deepen the knowledge about multimodal interaction (maternal touch, mother’s and infants’ vocalizations and infants’ gaze) between a mother and her twin preterm infants (twin 1 [female] and twin 2 [male]) during speech and humming in kangaroo care. A microanalytical case study was carried out using ELAN, PRAAT, and MAXQDA software. Descriptive and comparative analysis was performed using SPSS software. We observed: (1) significantly longer humming phrases to twin 2 than to twin 1 (p = 0.002), (2) significantly longer instances of maternal touch in humming than in speech to twin 1 (p = 0.000), (3) a significant increase in the pitch of maternal speech after twin 2 gazed (p = 0.002), and (4) a significant increase of pitch in humming after twin 1 vocalized (p = 0.026). This exploratory study contributes to questioning the role of maternal touch during humming in kangaroo care, as well as the mediating role of the infant’s gender and visual and vocal behavior in the tonal change of humming or speech.publishersversionpublishe

Pseudomonas spp. em répteis de companhia : um estudo retrospetivo

Dissertação de Mestrado Integrado em Medicina VeterináriaA manutenção de répteis como animais de companhia tem aumentado nos últimos anos na Europa. Estes animais podem ser reservatórios de microrganismos zoonóticos, incluindo bactérias do género Pseudomonas, cujos mecanismos de resistência aos antimicrobianos em constante evolução tornam as opções terapêuticas disponíveis atualmente cada vez mais ineficazes, pelo que a monitorização da relação entre este género bacteriano e a classe Reptilia é de grande importância. Este estudo retrospetivo teve como objetivos o cálculo da frequência de isolamento de Pseudomonas em répteis detidos como animais de companhia ou de coleção, apresentados a consulta no Hospital Escolar Veterinário (HEV) entre Abril de 2006 e Fevereiro de 2020; a identificação de fatores que podem predispor para infeção por Pseudomonas spp. em répteis; e a caracterização do perfil de resistência a antibióticos, através do método de difusão em disco (Kirby-Bauer), dos isolados. Para tal, procedeu-se à verificação dos relatórios de análises bacteriológicas do Laboratório de Microbiologia e Imunologia (LMI) da FMV-ULisboa e procedeu-se à pesquisa e recolha das fichas clínicas dos répteis através da plataforma Guruvet ®, tendo sido possível ter acesso às fichas de 24 animais, cujos dados foram utilizados para análise estatística. Verificou-se uma frequência de isolamento para Pseudomonas spp. de 35%. Não foi observada uma associação estatisticamente significativa entre o isolamento deste género bacteriano e a idade, género ou espécie dos répteis, nem com o tipo de doença apresentado pelos animais. No entanto, os perfis de multirresistência obtidos são preocupantes, com 4 isolados classificados como multirresistentes, 4 classificados como extensivamente resistentes e 1 classificado como pan-resistente. Estes resultados alertam para a imprescindibilidade da criação de uma política de administração de antibióticos em répteis, com uma hierarquização dos agentes, de forma a garantir uma prescrição segura e universal. A eficácia do tratamento de uma infeção cutânea grave causada por Pseudomonas sp., não suscetível a todos os antibióticos utilizados, através da aplicação de um antisséptico tópico, pode sugerir que esta abordagem seja válida noutros casos clínicos e uma opção adequada para tratamento empírico enquanto se aguarda pelos resultados do teste de sensibilidade aos antibióticos ou do cálculo da concentração mínima inibitória.ABSTRACT - PSEUDOMONAS SPP. IN COMPANION REPTILES: A RETROSPECTIVE STUDY - The maintenance of reptiles as pets has increased in recent years in Europe. These animals can be reservoirs of zoonotic microorganisms, including bacteria of the genus Pseudomonas, whose resistance mechanisms to antimicrobials in constant evolution make the therapeutic options available today increasingly ineffective, so monitoring the relationship between this bacterial genus and the Reptilia class is of great importance. This retrospective study aimed to calculate the frequency of isolation of Pseudomonas in reptiles kept as pets, presented for consultation at the Veterinary Teaching Hospital (HEV) between April 2006 and February 2020; the identification of factors that may predispose to infection by Pseudomonas spp. in reptiles; and the characterization of the antibiotic resistance profile, using the disk diffusion method (Kirby-Bauer), of the isolates. To achieve this, we proceeded to the verification of the bacteriological analysis reports of the Microbiology and Immunology Laboratory (LMI) of FMV-ULisboa and proceeded to the research and collection of the clinical records of the reptiles through the Guruvet ® platform, having been able to access to the records of 24 animals, whose data were used for statistical analysis. An isolation frequency for Pseudomonas spp. of 35% was obtained. There was no statistically significant association between the isolation of this bacterial genus and the age, genus or species of the reptiles, nor with the type of disease presented by the animals. However, the multi-resistance profiles obtained are worrying, with 4 isolates classified as multidrug-resistant, 4 classified as extensively drug resistant and 1 classified as pandrugresistant. These results alert to the necessity of creating a policy of antibiotics’ administration in reptiles, with a hierarchy of agents, in order to guarantee a safe and universal prescription. The effectiveness of the treatment of a severe skin infection caused by Pseudomonas sp., not susceptible to all antibiotics used, through the application of a topical antiseptic may suggest that this approach is valid in other clinical cases and an appropriate option for empirical treatment pending the results of the antibiotic sensitivity test or the calculation of the minimum inhibitory concentration.N/

Phytochemical Composition of Extractives in the Inner Cork Layer of Cork Oaks with Low and Moderate Coraebus undatus Attack

The beetle Coraebus undatus, during its larval stage feeds, and excavates galleries on the cork-generating layer of Quercus suber L. trees, seriously affecting the cork quality with significant economic losses for the cork industry. This work compared the composition of the extracts present in the innermost cork layers (the belly) of cork planks from Q. suber trees with low and moderate C. undatus attack in one stand. The total extractives in the inner cork layer from trees with moderate and low C. undatus attacks were similar (on average 22% of the cork mass) with a high proportion of polar compounds (91% of the total extractives). The chemical composition of the inner cork lipophilic extractives was the same in trees infested and free of larvae, with triterpenes as the most abundant family accounting for 77% of all the compounds, predominantly friedeline. The hydrophilic extractives differed on the levels of phenolic compounds, with higher levels in the inner cork extracts of samples from trees with low attack (90.0 mg GAE g1 vs. 59.0 mg GAE g1 of inner cork mass) The potential toxic activity of phenolic compounds may have a role in decreasing the larval feedinginfo:eu-repo/semantics/publishedVersio

Diferenças de género

UID/EAT/00693/2019authorsversionpublishe

Blood flow of bubbles moving in microchannels with bifurcations

The gas embolism is a well-known phenomenon. Previous studies have been performed to understand the formation, the behavior and the influence of air bubbles in microcirculation. This study aims to investigate the flow of bubbles in a microchannel network with bifurcations. For that purpose, a microchannel network was fabricated by soft lithography. The working fluids used were composed by sheep red blood cells (RBCs) suspended in dextran 40 and two different hematocrits were studied, 5% and 10%. The in vitro blood flow was analyzed for a flow rate of lO ~-tllmin, by using an inverted microscope and a high-speed camera. It was possible to visualize the formation of the bubbles and their behavior along the network. The results show that the passage of air bubbles influences the cells local concentration, since a higher concentration of cells was seen upstream to the bubble and lower concentrations downstream to the bubble.This work was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal, under the strategic grants UID/EMS/04077/2019, UID/EEA/04436/2019 and UID/EMS/00532/2019. The authors are also grateful for the partial funding of FCT through the projects POCI-01-0145-FEDER-016861 (ref: PTDC/QEQ-FIT/4287/2014), NORTE-01-0145- FEDER-029394, NORTE-01-0145-FEDER-030171, funded by COMPETE2020, NOR1E2020, PORTUGAL2020, and FEDER. D. Bento acknowledges the PhD scholarship SFRH/BD/911921 2012 granted by FCT.info:eu-repo/semantics/publishedVersio

Paraneoplastic necrotizing myopathy in a woman with breast cancer: a case report

<p>Abstract</p> <p>Introduction</p> <p>Paraneoplastic necrotizing myopathy is a rare disorder, described as a proximal, symmetrical, and rapidly progressing myopathy that is manifested as a paraneoplastic syndrome. Diagnosis is established via histological examination of the muscle biopsy.</p> <p>Case presentation</p> <p>We present the case of a 53-year-old woman, born in Guinea-Bissau, with a history of locally advanced breast cancer, diagnosed ten months previously. The patient had experienced a progressively proximal muscle weakness of the lower extremities, which led to a total inability to walk. Upon neurological examination, the patient showed muscle weakness and atrophy in both proximal lower extremities without myalgia. Muscle strength was graded according to the Medical Research Council Scale as 2 out of 5 in the bilateral iliopsoas muscle, and 4 out of 5 in the bilateral quadriceps femoris. The deep-tendon reflexes were hypoactive. The laboratory examination showed increased values of serum creatinine kinase and myoglobin. An electromyogram showed an incomplete interference pattern during voluntary contraction in the iliopsoas and quadriceps femoris. The motor nerve conduction was 44.1 m/s and 44.3 m/s in the right and left tibial nerves, respectively, and 46.5 m/s and 46.1 m/s in the right and left peroneal nerves, respectively. The sensory motor nerve conductions and the compound motor action potential amplitudes were normal. These findings, despite not being specific, suggested a myopathy. Consequently, a muscle biopsy was performed. A biopsy specimen showed myopathic changes that were characteristic of a necrotizing myopathy.</p> <p>Conclusion</p> <p>Treatment for this syndrome consists of controlling the tumor, and providing corticoid therapy. This led to the partial remission of the neurological manifestations.</p

Quality of life of elderly people attended by the Family Health Strategy

Objective: To assess the quality of life of elderly people assisted by the Family Health Strategy. Method: Cross-sectional, descriptive-exploratory and quantitative study conducted at the homes of elderly people in the urban area of Floriano - PI. The sample was composed of 671 elderly people. Data were collected by using WHOQOL-Bref and a questionnaire for social-demographic information. The data were processed with the Chi-square test and analysis of hierarchy grouping. Results: The majority of the study was composed of females (65,3%). Most of the elderly people surveyed lived without a spouse, with monthly income ranging between 1 and 3 minimum salaries and had very good quality of life (66,2%). Most of the latter were males (69,5%), with significant difference between genres (p=0.021). Conclusion: The majority of the elderly people surveyed had good quality of life according to scores from the dominions evaluated by WHOQOL-Bref, showing higher scores in the dominion of social relationships and lower scores in the dominion of environment

Sound-Music Experiences and Maternal Sensitivity Following Preterm Birth: Contributions for the Model of Family-Centered Care.

Introduction: The model of Family-Centered Care strengthens the parents’ active role at the caring for the newborn baby. Prenatal maternal sound-music sensitivity increases at the third trimester of pregnancy and shows to be a good predictor of prenatal maternal attachment. Method: preterm mothers (N = 36) were interviewed about clinical and sociodemographic features as well as about their sound-music experiences after and before preterm delivery. Correlations between sociodemographic, clinical and sound-music experiences were performed. Results: sound-music experiences after preterm birth were associated with memories of sound-music experiences during pregnancy and seem to be influenced by previous abortions as well as by babies’ variables (gestational age at birth, weight at birth and chronological age at observation). Conclusion: The assessment of maternal sound-music experiences following preterm birth may be useful in an innovative way to strength the model of Family-Centered Care underlining the mothers’ active role at the attention directed to the vulnerable baby

Psychosocial care center: convergence between mental and collective health

This paper aimed to discuss the existence of converging points between mental and collective health practices from the perspective of the professionals of a Psychosocial Care Center (CAPS) in Campinas, SP. The study conducted was both descriptive and qualitative and the data obtained were analyzed according to the theoretical basis coming from phenomenology and discourse analysis. The results showed the existence of factors that hinder the structuring of the health care network, such as: lack of physical resources, inadequate physical space and lack of knowledge regarding CAPS's role as well as the development of a work based on users embracement, on the development of individualized therapeutic projects, on matrix support and team meetings, enhancing a sense of collective and dialogical work.Este artículo tuvo como propuesta discutir la existencia de puntos de convergencia entre el trabajo de salud mental y la salud pública, desde la perspectiva de los profesionales que trabajan en un Centro de Atención Psicosocial (CAPS) en Campinas, SP. Este estudio es cualitativo y descriptivo, y la base teórica de la fenomenología y el análisis de contenido del discurso motivado el análisis de los datos obtenidos. Los resultados mostraron tanto factores que dificultan la articulación de la red de atención como la escasez de recursos materiales, espacio físico inadecuado y la falta de papel de CAPS, como también el desarrollo de un trabajo dirigido a la recepción, a la construcción de proyectos de tratamiento singulares, al apoyo matricial y a las reuniones de equipo, evidenciando acciones que potencian el trabajo colectivo y dialógico.Este trabalho teve por objetivo discorrer sobre a existência de pontos de convergência entre o trabalho de saúde mental e o de saúde coletiva, a partir da percepção de profissionais que atuam em um Centro de Atenção Psicossocial (CAPS) do município de Campinas, SP. Trata-se de estudo de natureza qualitativa e descritiva em que os referenciais teóricos relativos à fenomenologia e à análise do conteúdo do discurso fundamentaram a análise dos dados obtidos. Os resultados mostraram tanto fatores que dificultam a articulação da rede de cuidados - como escassez de recursos materiais, espaço físico inadequado e desconhecimento do papel do CAPS, quanto fatores que dificultam o desenvolvimento de um trabalho dirigido ao acolhimento, à construção de projetos terapêuticos singulares, ao matriciamento e às reuniões de equipe, evidenciando ações que potencializam o trabalho coletivo e dialógico.60361

USO DE OLEATO DE MONOETANOLAMINA PARA ESCLEROSE QUÍMICA EM PACIENTE COM HEMANGIOMA INTRA-ORAL: RELATO DE CASO

O hemangioma é considerado uma neoplasia vascular benigna em que a principal característica consiste na proliferação de vasos sanguíneos. Paciente de 58 anos, sexo feminino, leucoderma, encaminhada para a disciplina de Estomatologia da UFPR com queixa de “mancha no lábio inferior após mordiscamento”. Durante o exame intraoral observou-se uma mácula, de coloração arroxeada, sem sintomatologia dolorosa e com limites bem definidos, medindo cerca de 5x3 milímetros. O exame de diascopia positivo (vitropressão) evidenciou um esmaecimento da coloração arroxeada e estabeleceu-se o diagnóstico clínico de hemangioma. A localização, tamanho e condição sistêmica da paciente determinaram a indicação e conduta terapêutica com esclerose química da lesão. O tratamento consistiu na injeção de 0,05mL de agente esclerosante- Ethamolin® (oleato de monoetanolamina) em 4 sessões. No decorrer das aplicações, a região tratada apresentou-se fibrosa e com coloração mais próxima àquela da mucosa normal adjacente. Na última sessão foi aplicado apenas 0,01 mL para conclusão do tratamento. O presente relato reforça a indicação da literatura de que o tratamento esclerosante é de fácil execução, rápido e dispensa manobras invasivas. A semiotécnica de vitropressão contribui para o diagnóstico clínico e a aplicação de agente esclerosante resulta em um prognóstico favorável, com redução considerável da coloração original.