Effects of entomopathogenic nematodes on evolution of pink bollworm resistance to Bacillus thuringiensis toxin Cry1Ac

The evolution of resistance by pests can reduce the efficacy of transgenic crops that produce insecticidal toxins from Bacillus thuringiensis (Bt). However, fitness costs may act to delay pest resistance to Bt toxins. Meta-analysis of results from four previous studies revealed that the entomopathogenic nematode Steinernema riobrave (Rhabditida: Steinernematidae) imposed a 20% fitness cost for larvae of pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), that were homozygous for resistance to Bt toxin Cry1Ac, but no significant fitness cost was detected for heterozygotes. We conducted greenhouse and laboratory selection experiments to determine whether S. riobrave would delay the evolution of pink bollworm resistance to Cry1Ac. We mimicked the high dose/refuge scenario in the greenhouse with Bt cotton (Gossypium hirsutum L.) plants and refuges of non-Bt cotton plants, and in the laboratory with diet containing Cry1Ac and refuges of untreated diet. In both experiments, half of the replicates were exposed to S. riobrave and half were not. In the greenhouse, S. riobrave did not delay resistance. In the laboratory, S. riobrave delayed resistance after two generations but not after four generations. Simulation modeling showed that an initial resistance allele frequency \u3e0.015 and population bottlenecks can diminish or eliminate the resistance-delaying effects of fitness costs. We hypothesize that these factors may have reduced the resistance-delaying effects of S. riobrave in the selection experiments. The experimental and modeling results suggest that entomopathogenic nematodes could slow the evolution of pest resistance to Bt crops, but only under some conditions

Effects of four nematode species on fitness costs of pink bollworm resistance to Bacillus thuringiensis toxin Cry1Ac

Evolution of resistance by pests can reduce the efficacy of transgenic crops that produce insecticidal toxins from the bacterium Bacillus thuringiensis Berliner (Bt). In conjunction with refuges of non-Bt host plants, fitness costs can delay the evolution of resistance. Furthermore, fitness costs often vary with ecological conditions, suggesting that agricultural landscapes can be manipulated to magnify fitness costs and thereby prolong the efficacy of Bt crops. In the current study, we tested the effects of four species of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) on the magnitude and dominance of fitness costs of resistance to Bt toxin CrylAc in pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae). For more than a decade, field populations of pink bollworm in the United States have remained susceptible to Bt cotton Gossypium hirsutum L. producing CrylAc; however, we used laboratory strains that had a mixture of susceptible and resistant individuals. In laboratory experiments, dominant fitness costs were imposed by the nematodeSteinernema riobrave Cabanillas, Poinar, and Raulston but no fitness costs were imposed bySteinernema carpocapsae Weiser, Steinernema sp. (ML18 strain), or Heterorhabditis sonorensis Stock, Rivera-Orduño, and Flores-Lara. In computer simulations, evolution of resistance to CrylAc by pink bollworm was substantially delayed by treating some non-Bt cotton refuge fields with nematodes that imposed a dominant fitness cost, similar to the cost observed in laboratory experiments with S. riobrave. Based on the results here and in related studies, we conclude that entomopathogenic nematodes could bolster insect resistance management, but the success of this approach will depend on selecting the appropriate species of nematode and environment, as fitness costs were magnified by only two of five species evaluated and also depended on environmental factors

Effects of Pink Bollworm Resistance to Bacillus thuringiensis on Phenoloxidase Activity and Susceptibility to Entomopathogenic Nematodes

Widespread planting of crops genetically engineered to produce insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) imposes selection on many key agricultural pests to evolve resistance to Bt. Fitness costs can slow the evolution of Bt resistance. We examined effects of entomopathogenic nematodes on fitness costs of Bt resistance in the pink bollworm,Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), a major pest of cotton,Gossypium hirsutum L., in the southwestern United States that is currently controlled by transgenic cotton that produces Bt toxin Cry1Ac. We tested whether the entomopathogenic nematodes Steinernema riobrave Cabanillas, Poinar, and Raulston (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae) affected fitness costs of resistance to Cry1Ac in two laboratory-selected hybrid strains of pink bollworm reared on non-Bt cotton bolls. The nematode S. riobrave imposed a recessive fitness cost for one strain, and H. bacteriophora imposed a fitness cost affecting heterozygous resistant individuals for the other strain. Activity of phenoloxidase, an important component of insects\u27 immune response, did not differ between Bt-resistant and Bt-susceptible families. This suggests phenoloxidase does not affect susceptibility to entomopathogenic nematodes in Bt-resistant pink bollworm. Additionally, phenoloxidase activity does not contribute to Bt resistance, as has been found in some species. We conclude that other mechanisms cause higher nematode-imposed mortality for pink bollworm with Bt resistance genes. Incorporation of nematode-imposed fitness costs into a spatially explicit simulation model suggests that entomopathogenic nematodes in non-Bt refuges could delay resistance by pink bollworm to Bt cotton

GSK3-mediated raptor phosphorylation supports amino acid-dependent Q2 mTORC1-directed signalling

The mammalian or mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) is a ubiquitously expressed multimeric protein kinase complex that integrates nutrient and growth factor signals for the co-ordinated regulation of cellular metabolism and cell growth. Herein, we demonstrate that suppressing the cellular activity of glycogen synthase kinase-3 (GSK3), by use of pharmacological inhibitors or shRNA-mediated gene silencing, results in substantial reduction in amino acid (AA)-regulated mTORC1-directed signalling, as assessed by phosphorylation of multiple downstream mTORC1 targets. We show that GSK3 regulates mTORC1 activity through its ability to phosphorylate the mTOR-associated scaffold protein raptor (regulatory-associated protein of mTOR) on Ser(859). We further demonstrate that either GSK3 inhibition or expression of a S859A mutated raptor leads to reduced interaction between mTOR and raptor and under these circumstances, irrespective of AA availability, there is a consequential loss in phosphorylation of mTOR substrates, such as p70S6K1 (ribosomal S6 kinase 1) and uncoordinated-51-like kinase (ULK1), which results in increased autophagic flux and reduced cellular proliferation

Large-scale magnetic field in the Monoceros OB 1 east molecular cloud

Context. The role of large-scale magnetic fields in the evolution of star-forming regions remains elusive. Its investigation requires the observational characterization of well-constrained molecular clouds. The Monoceros OB 1 molecular cloud is a large complex containing several structures that have been shown to be engaged in an active interaction and to have a rich star formation history. However, the magnetic fields in this region have only been studied on small scales. Aims. We study the large-scale magnetic field structure and its interplay with the gas dynamics in the Monoceros OB 1 east molecular cloud. Methods. We combined observations of dust polarized emission from the Planck telescope and CO molecular line emission observations from the Taeduk Radio Astronomy Observatory 14-metre telescope. We calculated the strength of the plane-of-sky magnetic field using a modified Chandrasekhar-Fermi method and estimated the mass-over-flux ratios in different regions of the cloud. We used the comparison of the velocity and intensity gradients of the molecular line observations with the polarimetric observations to trace dynamically active regions. Results. The molecular complex shows an ordered large-scale plane-of-sky magnetic field structure. In the northern part, it is mostly orientated along the filamentary structures, while the southern part shows at least two regions with distinct magnetic field orientations. Our analysis reveals a shock region in the northern part right between two filamentary clouds that, in previous studies, were suggested to be involved in a collision. The magnetic properties of the north-main and north-eastern filaments suggest that these filaments once formed a single one, and that the magnetic field evolved together with the material and did not undergo major changes during the evolution of the cloud. In the southern part, we find that either the magnetic field guides the accretion of interstellar matter towards the cloud or it is dragged by the matter falling towards the main cloud. Conclusions. The large-scale magnetic field in the Monoceros OB 1 east molecular cloud is tightly connected to the global structure of the complex. In the northern part, it seems to serve a dynamically important role by possibly providing support against gravity in the direction perpendicular to the field and to the filament. In the southern part, it is probably the most influential factor governing the morphological structure by guiding possible gas inflow. A study of the whole Monoceros OB 1 molecular complex at large scales is necessary to form a global picture of the formation and evolution of the Monoceros OB 1 east cloud and the role of the magnetic field in this process.Peer reviewe

Influence of agricultural practices and ecological environment on the diversity of the fallows of forest origin (Highlands, Madagascar)

The present study relates to the fallow (kapoka) of forest origin in western edge of the forested “corridor” Ranomafana-Andringitra on the Malagasy Highlands. The objectives of this research were to characterize the evolution of the vegetation in the fallows and to describe the cultivation parameters and those related to the ecological environment in order to determine those which could infl uence the dynamics of succession. A synchronic approach was adopted: 14 fallow fields of different ages were retained. Formal investigations and pedological studies were carried out in the fields. The results showed that: (1) the fallow vegetation can be in a progressive evolution leading to a shrubby and wooded vegetation when intensity of exploitation, characterized by the cumulated duration of the cultivated period, mode of tillage, and the age of the fallow, is weak; on the other hand when intensity of exploitation is high the regressive evolution leading to a grassy formation of pseudo-steppic type occurs; (2) vegetation diversity within each field in fallow is low (from 3 to 41 species), whereas when scale is changed, total diversity for the 14 fields is much more important (107 species)La présente étude concerne les jachères (kapoka) d'origine forestière en lisière ouest du « corridor » forestier Ranomafana-Andringitra sur les Hautes-Terres malgaches. Les objectifs de cette recherche sont de caractériser l'évolution de la végétation dans les jachères et de décrire les paramètres culturaux et liés à la station, afin de déterminer ceux qui pourraient influencer la dynamique de succession. Une approche synchronique a été adoptée: 14 parcelles d'âges différents ont été retenues. Des enquêtes formelles et des études pédologiques ont été effectuées dans les parcelles. Il ressort des résultats que: (1) la végétation des jachères peut évoluer d'une manière progressive si l'intensité d'exploitation, caractérisée par la durée cumulée de la phase culture, le mode de préparation du sol, et l'âge de la jachère, est faible, sinon, c'est l'évolution régressive aboutissant à une formation herbeuse de type pseudo-steppe qui l'emporte; (2) la diversité végétale au sein de chaque parcelle en jachère est faible (de 3 à 41 espèces), alors que si l'on change d'échelle, la diversité totale pour les 14 parcelles est beaucoup plus importante (107 espèces)

PV1 Down-Regulation via shRNA Inhibits the Growth of Pancreatic Adenocarcinoma Xenografts

PV1 is an endothelial-specific protein with structural roles in the formation of diaphragms in endothelial cells of normal vessels. PV1 is also highly expressed on endothelial cells of many solid tumours. On the basis of in vitro data, PV1 is thought to actively participate in angiogenesis. To test whether or not PV1 has a function in tumour angiogenesis and in tumour growth in vivo, we have treated pancreatic tumour-bearing mice by single-dose intratumoural delivery of lentiviruses encoding for two different shRNAs targeting murine PV1. We find that PV1 down-regulation by shRNAs inhibits the growth of established tumours derived from two different human pancreatic adenocarcinoma cell lines (AsPC-1 and BxPC-3). The effect observed is because of down-regulation of PV1 in the tumour endothelial cells of host origin, PV1 being specifically expressed in tumour vascular endothelial cells and not in cancer or other stromal cells. There are no differences in vascular density of tumours treated or not with PV1 shRNA, and gain and loss of function of PV1 in endothelial cells does not modify either their proliferation or migration, suggesting that tumour angiogenesis is not impaired. Together, our data argue that down-regulation of PV1 in tumour endothelial cells results in the inhibition of tumour growth via a mechanism different from inhibiting angiogenesis

Rheological characterization of jet-cooked Lesquerella fendleri seed gum and cornstarch solutions

Abstract Lesquerella is a potential new seed crop that contains hydroxy fatty acid triglycerides and approximately 15% seed coat gums. The polysaccharide gum of the Lesquerella fendleri seed was isolated and jet-cooked with cornstarch in a series of laboratory experiments to investigate the viscoelastic properties of gum-modified starch solutions. The Lesquerella gum was combined with cornstarch at 1, 5, and 10% levels to produce a jet-cooked and drum dried material with potential application as a thickening or suspension agent. Flow curves were determined for 1% solutions of jet-cooked starch and gum combinations. All materials investigated were biodegradable and exhibited positive thixotropic behavior

GEM DETECTORS FOR THE TRANSVERSE PROFILE MEASUREMENT OF LOW ENERGY ANTIPROTONS AND HIGH ENERGY HADRONS

Abstract Gas Electron Multipliers (GEM) are finding more and more applications in beam instrumentation

Cross-sectional and prospective correlates of recovery expectancies in the rehabilitation of whiplash injury

Investigations have shown that expectancies are significant prognostic indicators of recovery outcomes following whiplash injury. However, little is currently known about the determinants of recovery expectancies following whiplash injury. The purpose of the present study was to examine the cross-sectional and prospective correlates of recovery expectancies in individuals admitted to a rehabilitation program for whiplash injury.Participants (N=96) completed measures of recovery expectancies, psychosocial variables, symptom severity, symptom duration, and disability at time 0 (admission) and time 1 (discharge).Consistent with previous research, more positive recovery expectancies at time 0 were related to reductions in pain at time 1 (r=-0.33,